LOCUS       sequence-310709-        2038 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of c-luciferase from sp. Maristella sp. SVU (Belize).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Hensley NM, Ellis EA, Leung NY, Coupart J, Mikhailovsky A, Taketa 
            DA, Tessler M, Gruber DF, De Tomaso AW, Mitani Y, Rivers TJ, Gerrish
            GA, Torres E, Oakley TH
  TITLE     Selection, drift, and constraint in cypridinid luciferases and the 
            diversification of bioluminescent signals in sea fireflies.
  JOURNAL   Mol Ecol. 2020 Oct 8. doi: 10.1111/mec.15673.
  PUBMED    33031624
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ecol. 
            2020 Oct 8. doi: 10.1111/mec.15673."
COMMENT     Sequence Label: pPICZ-aC-MSVU-BZ
FEATURES             Location/Qualifiers
     source          1..2038
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1..57,58..255)
                     /codon_start=1
                     /gene="MF-alpha-1"
                     /product="N-terminal secretion signal from S. cerevisiae 
                     alpha-factor"
                     /label=alpha-factor secretion signal
                     /note="Cleavage by the Kex2 protease occurs after the 
                     dibasic KR sequence."
                     /translation="MRFPSIFTAVLFAASSALAAPVNTTTEDETAQIPAEAVIGYSDLE
                     GDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVSLEKR"
     CDS             1921..1950
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1966..1983
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgagatttc cttcaatttt tactgctgtt ttattcgcag catcctccgc attagctgct
       61 ccagtcaaca ctacaacaga agatgaaacg gcacaaattc cggctgaagc tgtcatcggt
      121 tactcagatt tagaagggga tttcgatgtt gctgttttgc cattttccaa cagcacaaat
      181 aacgggttat tgtttataaa tactactatt gccagcattg ctgctaaaga agaaggggta
      241 tctctcgaga agagagaggc tcaagattgt tatgaattca cactcgagaa gagagaggct
      301 caagattgtt atgaattcac atgggcttcg aatgattttc catcttcatg tgaggctata
      361 aacggaagat gtgtagactc tgcgtgcggt agctgtgacg aagttctgtt tggcgatgga
      421 ttgtgtgaaa atgccggagg agcatctccc aagtgctgta gagattgccc agaaatcgtc
      481 aggtgcagag cttcagcagc tggatttttt cacacgttct atgggcagag attcaatctc
      541 caggtaccag ggacctatct gctgagtgaa gactgcgtgg gaggactatg gtctctatat
      601 gttaacctgg cgaatataga aggagagaag ggagccgttt tagattccgt taagatggtt
      661 gttggagacg taactctaga tattaagcag aaagttggat ctattacggt aaatggaggt
      721 tctgttgaaa tcgacagcaa tccattctct atcggtgacg tcaccatcgc cgtcgtatac
      781 actccttact ttagcgtctc cgtcatcgag tttcttaagc ttgttacatt tgatatctta
      841 caaggcagag ctttccgact tgctccagac ttcttatacg ctgataggac ctgtggctta
      901 tgtggattga tgtccgatga accttctgat tttattgacg atccagatca acttgcaatc
      961 caagacataa tgaatcaaga tgtagaaggc tgcccactat ctggaaatcc ttctgacgcc
     1021 gagtattgct tgaataagat gcagccatat aaggacggct gtattaacaa gaacgatgtt
     1081 cacttcggta cctacctgta cgcatgcgca ctggcctact gcatgggcgg cgacgataga
     1141 gtagaggatg ttatttttga atacgccgaa gcctgcgttg aaccaatcgg tagagcaacc
     1201 tgcgttatga atggacacac atactatgac acattcgaca aatcaagtta tcagtttcag
     1261 gcaccttgta aggttctatt cgcgaaagac tgtacagatg atgaatggga agtaactatt
     1321 actcataaag aagctggaga atatactgaa gtagaaaagg tcaccgtccg gtatttccaa
     1381 accttgattg atctggtagc agagaataag aaagtttttg ttaatggaac tgaagttagc
     1441 gtcccttaca actatggaga tacatctata tacatgtatg ataacttagt aactacagca
     1501 gttctaccag gagcagtggt tgtcaaatac aacttcgagc agatgttggc tctacacatt
     1561 agagatccat atgaagcaga cagctgtggt ttatgtggta tttgggattt ggataaatct
     1621 aatgatggtc ctgatgctca gcatgcagat tgtgaaccaa ctcccaaccc tcctacatgc
     1681 actgctgaca aagaggctga agctagagaa ctctgccaga atatgtttcc tgcttctatc
     1741 gacgacaaat gtaacatctg ctacaaggct gaccgtgttg agcgctgtat gtatgagtac
     1801 tgtcttggtg gtctagaagc attctgtcaa catgctggta ccgtcatcga tgaatgcttc
     1861 gtgaggcacg gagacgacct acaatatccc cctcagtgta aagcggccgc cagctttcta
     1921 gaacaaaaac tcatctcaga agaggatctg aatagcgccg tcgaccatca tcatcatcat
     1981 cattgagttt gtagccttag acatgactgt tcctcagttc aagttgggca cttacgag
//
