LOCUS       sequence-310717-         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BioBrick mammalian-codon-optimized dsRed.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Bao L, Menon PNK, Liljeruhm J, Forster AC
  TITLE     Overcoming chromoprotein limitations by engineering a red 
            fluorescent protein.
  JOURNAL   Anal Biochem. 2020 Sep 3:113936. doi: 10.1016/j.ab.2020.113936.
  PUBMED    32891596
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Anal 
            Biochem. 2020 Sep 3:113936. doi: 10.1016/j.ab.2020.113936."
COMMENT     Sequence Label: pSB1C3-J23110-B0034-dsRed
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    1..22
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        23..57
                     /label=BBa_J23110
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.33"
     RBS             66..77
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(84..86,87..761)
                     /codon_start=1
                     /product="wild-type DsRed"
                     /label=DsRed1
                     /note="mammalian codon-optimized"
                     /translation="MRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(264..284)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     482..501
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     676..699
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    790..810
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
ORIGIN
        1 gaattcgcgg ccgcttctag agtttacggc tagctcagtc ctaggtacaa tgctagctac
       61 tagagaaaga ggagaaatac tagatgcgct cctccaagaa cgtcatcaag gagttcatgc
      121 gcttcaaggt gcgcatggag ggcaccgtga acggccacga gttcgagatc gagggcgagg
      181 gcgagggccg cccctacgag ggccacaaca ccgtgaagct gaaggtgacc aagggcggcc
      241 ccctgccctt cgcctgggac atcctgtccc cccagttcca gtacggctcc aaggtgtacg
      301 tgaagcaccc cgccgacatc cccgactaca agaagctgtc cttccccgag ggcttcaagt
      361 gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc
      421 tgcaagacgg ctgcttcatc tacaaggtga agttcatcgg cgtgaacttc ccctccgacg
      481 gccccgtaat gcagaagaag actatgggct gggaggcctc caccgagcgc ctgtaccccc
      541 gcgacggcgt gctgaagggc gagatccaca aggccctgaa gctgaaggac ggcggccact
      601 acctggtgga gttcaagtcc atctacatgg ccaagaagcc cgtgcagctg cccggctact
      661 actacgtgga ctccaagctg gacatcacct cccacaacga ggactacacc atcgtggagc
      721 agtacgagcg cacggagggc cgccaccacc tgttcctgta gtaacgctga tagtgctagt
      781 gtagatcgct actagtagcg gccgctgcag
//
