LOCUS       sequence-310719-         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BioBrick mammalian-codon-optimized mRFP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Bao L, Menon PNK, Liljeruhm J, Forster AC
  TITLE     Overcoming chromoprotein limitations by engineering a red 
            fluorescent protein.
  JOURNAL   Anal Biochem. 2020 Sep 3:113936. doi: 10.1016/j.ab.2020.113936.
  PUBMED    32891596
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Anal 
            Biochem. 2020 Sep 3:113936. doi: 10.1016/j.ab.2020.113936."
COMMENT     Sequence Label: pSB1C3-J23110-B0034-mRFP1
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    1..22
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        23..57
                     /label=BBa_J23110
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.33"
     RBS             66..77
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             84..761
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             84..761
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(214..232)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     482..501
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     676..699
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    790..810
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
ORIGIN
        1 gaattcgcgg ccgcttctag agtttacggc tagctcagtc ctaggtacaa tgctagctac
       61 tagagaaaga ggagaaatac tagatggcct cctccgagga cgtcatcaag gagttcatgc
      121 gcttcaaggt gcgcatggag ggctccgtga acggccacga gttcgagatc gagggcgagg
      181 gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggcggcc
      241 ccctgccctt cgcctgggac atcctgtccc ctcagttcca gtacggctcc aaggcctacg
      301 tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt
      361 gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc
      421 tgcaagacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg
      481 gccccgtaat gcagaagaag accatgggct gggaggcctc caccgagcgg atgtaccccg
      541 aggacggcgc cctgaagggc gagatcaaga tgaggctgaa gctgaaggac ggcggccact
      601 acgacgccga ggtcaagacc acctacatgg ccaagaagcc cgtgcagctg cccggcgcct
      661 acaagaccga catcaagctg gacatcacct cccacaacga ggactacacc atcgtggaac
      721 agtacgagcg cgccgagggc cgccactcca ccggcgccta ataacgctga tagtgctagt
      781 gtagatcgct actagtagcg gccgctgcag
//
