LOCUS       sequence-31072-e         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  TITLE     The Parkinson Disease-associated Leucine-rich Repeat Kinase 2 
            (LRRK2) Is a Dimer That Undergoes Intramolecular Autophosphorylation
  JOURNAL   J Biol Chem. 2008 Jun 13;283(24):16906-14.
  PUBMED    18397888
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2008-06-13"; volume: "283(24)"; pages: "16906-14"
COMMENT     Sequence Label: 2XMyc-LRRK2-3XKD/Y1699C
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        45..63
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      96..105
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             105..134
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             150..179
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    198..222
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttagtgaacc gtcagatccg cttagcgtat tacgccaagc tcgaaattaa ccctcactaa
       61 agggaacaaa agcatggagc tccaccgcgg tggcggccgc catggagcag aaactcatct
      121 ctgaagagga tctgagcccg ggcggatccg agcagaaact catctctgaa gaggatctgc
      181 tgcaggaatt cgatatcaca agtttgtaca aaaaagcagg ctccgaattc gcccttatgg
      241 ctagtggcag ctgtcagggg tgcgaagagg acgaggaaac tctgaagaag ttgatagtca
      301 ggctgaacaa tgtccaggaa ggaaaacaga tagaaacgct ggtccaaatc ctggaggatc
      361 tgctggtgtt cacgtactcc gagcacgcct ccaagttatt tcaaggcaaa aatatccatg
      421 tgcctctgtt gatcgtcttg gactcctata tgagagtcgc gagtgtgcag caggtgggtt
      481 ggtcacttct gtgcaaatta atagaagtct gtccaggtac aatgcaaagc ttaatgggac
      541 cccaggatgt tggaaatgat tgggaagtcc ttggtgttca ccaattgatt cttaaaatgc
      601 taacagttca taatgccagt gtaaacttgt cagtgattgg actgaagacc ttagatctcc
      661 tcctaacttc aggtaaaatc accttgctga tattggatga agaaagtgat attttcatgt
      721 taatttttga tgccatgcac tcatttccag ccaatgatga agtccagaaa cttggatgca
      781 aagctttaca tgtgctgttt gagagagtct cagaggagca actgactgaa tttgttgaga
      841 acaaagatta tatgatattg t
//
