LOCUS       sequence-310721-        8266 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CckA FRET Sensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8266)
  AUTHORS   Duvall SW, Childers WS
  TITLE     Design of a Histidine Kinase FRET Sensor to Detect Complex Signal 
            Integration within Living Bacteria.
  JOURNAL   ACS Sens. 2020 Jun 26;5(6):1589-1596. doi: 
            10.1021/acssensors.0c00008. Epub 2020 Jun 16.
  PUBMED    32495620
REFERENCE   2  (bases 1 to 8266)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssensors.0c00008"; journalName: "ACS Sens"; date: 
            "2020-06-26- 26"; volume: "5"; issue: "6"; pages: "1589-1596"
COMMENT     Sequence Label: pSWD95
FEATURES             Location/Qualifiers
     source          1..8266
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            29..138
                     /note="incP origin of transfer"
     primer_bind     290..306
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      307..353
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4034..4055
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5655..6359
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby2, with improved brightness and photostability (Bajar 
                     et al., 2016)"
                     /label=mRuby3
                     /translation="SKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTMRI
                     KVIEGGPLPFAFDILATSFMYGSRTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGGVV
                     TVTQDTSLEDGELVYNVKVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTDIA
                     LKVDGGGHLHCNFVTTYRSKKTVGNIKMPGVHAVDHRLERIEESDNETYVVQREVAVAK
                     YSNLGGGMDELYK"
     primer_bind     complement(6378..6394)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6405..6422)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6405..6421)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6414..6436)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(6619..7207)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6699..6718)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             7533..8192
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(7599..7618)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 cccccccccc accgcggtgg cggccgcccc ggccagcctc gcagagcagg attcccgttg
       61 agcaccgcca ggtgcgaata agggacagtg aagaaggaac acccgctcgc gggtgggcct
      121 acttcaccta tcctgcccgg ctgacgccgt tggatacacc aaggaaagtc tacacgaacc
      181 ctttggcaaa atcctgtata tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata
      241 atgaccccga agcagggtta tgcagcggaa aagatccgtc gacggccgct ctagaactag
      301 tggatccaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
      361 ttgtcgcatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat
      421 gccgtttgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca gtttttatat
      481 aagcttagcg ctaccggact cagatccaga tcgaaatgta ccggctgaac tggtggaagc
      541 acgttgagcc tcgtcaagga ctcatgaagc gcgtccctgc tctcagtcgc ctgctcgtta
      601 cgcctgaggt gtcaaaacac cgtctgttca tttggctaga cgctagagtt cttccggatc
      661 acaagctcca ggtcgttaca ctcgatgacg actgctcatt cggagtttta cactcaaggt
      721 tccacgaggt gtgggctctt gcagcggggt cgtggcacgg ctcaggcaat gatccgcgct
      781 acaccatctc caccaccttc gagaccttcc ccttcccgga aggcctgacg cccaacatag
      841 ccgccgtcga ctatgagggc gacccgcgcg cccaggccat cgccgcggcg gcggccgagc
      901 tgaaccggct gcgggaagcc tggctcaatc cgcccgacct ggtgcggatc gagcccgagg
      961 tcgtgcccgg ctatcccgac cgcgtgctgc ccgtcagccc cgaggcgggc gccgagctga
     1021 agaagcgcac cctgaccaac ctctacaacc agcgcccggc ctggctggac atggcccacc
     1081 agcgcctgga cgccgccgtc gccgccgcct acggctggcc cgacggcctg accgacgacg
     1141 agatcctgga gcgcctgttc gccctcaacc aggagcgcgc ggcggcgggg cggtaggtta
     1201 gacccctcca aaaccccgac ctccccggcg aaggccgggg tccagttaca tccggaacgc
     1261 ttggagtggg cccgcctacg gcctcgacca acccgcagtc ggcaaggggt tcgatctgga
     1321 ccccggcatt cgccggggag gtcggcagag aggtcagaac cgcgtcttgc agccctgctt
     1381 cagatgaacc ccgcgcagga agccgcggcg ggccaggccg gcgtcgacgg cggcgttcat
     1441 ctggcggtcg gcgggggcag cgccggtgac ttcgcgcacc aggccccgga acgggatgat
     1501 gctgttgatc agggtcttgc caccggcctc ggccagcttg ccggctcggt tttcggcgta
     1561 ctcggcgccg accttgtagt cgggccccag cgccgcgttc agcgccttca cctcattggc
     1621 cagggcctgg caggacttca gacccgcgag gctgtagggg tcgtcaaagg ccttggccag
     1681 gaccggcggc acctcgcgct tggacaggcc gacgtcgcgc accggctggg tgccgatgtc
     1741 gacggccttc ttgcccgcct cgccccagcg gccggacggt tcctcgggct tggccggcgg
     1801 cttgacctgg gcgtgggcgc tggaggccag cagcacggcg gcggctccgg ccatgatcag
     1861 cagcttgttc ggcatcaaaa gcgccctccc gacaaaacac gccacgggaa cggcaagcct
     1921 cgccgccggt tcccgcccgc aagtccctcg gcacgaactc taaggcctgc gcacgatcca
     1981 tcgccaccac aaagcgtgtc atcccggccg aagcgcagcg gagagccggg acccagggcc
     2041 acaagcacgg cgcttcgtca ccccctgggt cccggatagc gctccgcgct tccgggatga
     2101 cacgggttta agcacttggg tgagaagccc tagttcggcg cgccgcccgc ttccaggtcg
     2161 aacgcggcgt cgaaggcgtc gtcgccagga accgtccctt ccagggcggc gtcgaacagg
     2221 tcttcgagcg atgaccagat gaaggccatc tgctccatga tcgtcgggac gctggccgga
     2281 tcgaagccgg ggtagaggtc ttccaccagc cacaggcccg tgccgggatc gaaggcgaag
     2341 cggcagccga tcaggcggaa ctgggcgcgg gccatggtct cgaacagggc cgtcaggtcg
     2401 cgggcggcgt ccaggtcgtc gtggtccagc accaggcagc ggatctgcag ccagccgtgg
     2461 tcgggcagca ggtagaaggc gccctcgtcc tgatcctcgc ccgaaacctc cagcccccgg
     2521 tcgatggctt cgacgacata gccggccgcg cggcaggtgt cggtgagcgc ggccagcagg
     2581 gcggcttcct ggtcaggggt caggtcggtc atgggcaaga ggtccaggtc gtggtttgtc
     2641 ggcggcttct agcatggacc gcccgcgccc gtgaggccga ggatttcgcg ctggtcagac
     2701 aacctacttg ccgtccccac atgttagcgc taccaagtgc cgacgaacgc gcgccgccga
     2761 cggtgtcggc gcttcagacg ctcgagtttt ggggagacga ccatatggcc gacttgcagc
     2821 tccaggacaa ggtttcgacc ggcgccccgc gtcggcggtt tgatccatgg ctggtcggcg
     2881 cggcggtgtt tttcgtggct gcggcggccc tctcggcggc gccggcgctc aaggccggac
     2941 cgaccaccct ggccggcctg ctgctgcttc tgggcgtcgc gggcgtggct gtgttgggcc
     3001 ttgtcgccat tcgcggctca gcgctttccg gcggcgacgc cgaccaggct gaggggttca
     3061 tcgaggcgct ggccgagccg gccgccctgg ccgccgccga cggtcgcgtc ctggccgcca
     3121 acgggccctg gcgcgaagtc atgggcgaac agcgccgcct gcccaagggc gtggcgggct
     3181 ccagcctgtt tgcggccctg gtccaggcgc gccaggggca gatggccgag ggcatgctga
     3241 gcgctggagg aaccgactat accgccaagg tctcgcgcct tgcgggcgga cggctgatga
     3301 tccggcttgc gcccatcgtt gtcgctgagc cggttgtgga agacgcgtcg tcgaacgtta
     3361 cgcgtcaccg gtcggccacc gagatgagca agggcgagga gctgttcacc ggggtggtgc
     3421 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtc cgcggcgagg
     3481 gcgagggcga tgccaccaac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     3541 tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta cggcgtggcc tgcttcagcc
     3601 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     3661 tccaggagcg caccatctct ttcaaggacg acggtaccta caagacccgc gccgaggtga
     3721 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     3781 acggcaacat cctggggcac aagctggagt acaacttcaa cagccactac gtctatatca
     3841 cggccgacaa gcagaagaac tgcatcaagg ctaacttcaa gatccgccac aacgttgagg
     3901 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     3961 tgctgctgcc cgacaaccac tacctgagcc atcagtccaa gctgagcaaa gaccccaacg
     4021 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccctggaa agcggcggcg
     4081 aagatccgat ggtgagcccg gccccggtcg ccgagcgcgc cgccccgcca cccagctcgc
     4141 tggacgcctt cgccggagcc tcgccgttcg gcgcggccct gctggaaggc ctggagccgt
     4201 tcacctcgcg ggtgctggag accaatccgg cgctgaccac gatgaccggc gccaaggccg
     4261 gtgtgctgtt cggcgatctg atcgacgccg cctcgcgcgc cgaggccgag acgcgcctga
     4321 acgaaggccg cgccggtccc tacgaggtgc ggttggcgcg cgatccgtcg cgcatcgctc
     4381 acctttacct ctatcgcgcc gaaggccgcc tggtggccta catgatcgac gtgtccgagc
     4441 agaagcagat cgagctgcag ctgtcccagg cccagaagat gcaggccatc ggccagctgg
     4501 ccggcggcgt cgcgcacgac ttcaacaacc tcttgaccgc catccagctg cgtctggacg
     4561 agttgctgca tcgccatccc gtcggtgatc cgtcgtacga gggtctcaac gagatccgtc
     4621 agacgggcgt gcgcgccgcc gacctcgtgc gcaagctctt ggctttctcg cgcaagcaga
     4681 ccgtgcagcg cgaggtgctg gatctgggcg agctgatcag cgagttcgag gtcttgctgc
     4741 gccgcctgct gcgcgaagac gtcaagctga tcaccgacta tggccgcgac ctgccgcagg
     4801 tgcgcgccga caagagccag ctcgagacgg cggtcatgaa cctggccgtc aacgcccgcg
     4861 acgccgtgcg cgcggccaag ggcggcggcg tcgtgcgcat ccgcaccgcg cgcctgaccc
     4921 gcgacgaggc gatccagctg ggtttcccgg ccgccgacgg cgacacggcc ttcattgagg
     4981 tcagtgacga tggtccgggc attccgcccg acgtcatggg caagatcttc gacccgttct
     5041 tcaccaccaa gccggtgggc gagggtacgg gcctaggcct agccacggtc tatggcatcg
     5101 ttaagcagag cgacggctgg attcacgtcc acagccgtcc gaacgaaggc gcggccttcc
     5161 gcatcttcct gccggtctat gaagcgcccg ccggcgcggt cgccgtccag gccgtcgccg
     5221 agcccgccaa gccgcgcgcc gctcgcgacc tgtcgggcgc cggccgcatc ctgttcgtcg
     5281 aggacgagga cgccgtgcgc agcgtcgccg cccgcctgct gcgcgcccgt ggctacgagg
     5341 tgcttgaggc ggccgacggc gaagaggccc tgatcatcgc cgaggaaaac gccggcacga
     5401 tcgacctctt gatcagcgac gtgatcatgc ccggcatcga cggcccgacc ttgctgaaga
     5461 aggcgcgtgg ctatctgggg accgcgccgg tgatgttcat ctccggctat gccgaggccg
     5521 agttcagcga cctcttggaa ggcgagacgg gcgtgacctt cctccccaag ccgatcgaca
     5581 tcaagaccct ggccgagcgc gtcaagcagc agctgcaggc ggcgtcgaac gttacgcgtc
     5641 accggtcggc cacctctaag ggcgaagagc tgatcaagga aaatatgcgt atgaaggtgg
     5701 tcatggaagg ttcggtcaac ggccaccaat tcaaatgcac aggtgaagga gaaggcagac
     5761 cgtacgaggg agtgcaaacc atgaggatca aagtcatcga gggaggaccc ctgccatttg
     5821 cctttgacat tcttgccacg tcgttcatgt atggcagccg tacctttatc aagtacccgg
     5881 ccgacatccc tgatttcttt aaacagtcct ttcctgaggg ttttacttgg gaaagagtta
     5941 cgagatacga agatggtgga gtcgtcaccg tcacgcagga caccagcctt gaggatggcg
     6001 agctcgtcta caacgtcaag gtcagagggg taaactttcc ctccaatggt cccgtgatgc
     6061 agaagaagac caagggttgg gagcctaata cagagatgat gtatccagca gatggtggtc
     6121 tgagaggata cactgacatc gcactgaaag ttgatggtgg tggccatctg cactgcaact
     6181 tcgtgacaac ttacaggtca aaaaagaccg tcgggaacat caagatgccc ggtgtccatg
     6241 ccgttgatca ccgcctggaa aggatcgagg agagtgacaa tgaaacctac gtagtgcaaa
     6301 gagaagtggc agttgccaaa tacagcaacc ttggtggtgg catggacgag ctgtacaagt
     6361 aaagctgcag cccgggggat ccactagttc tagagcggcc attcactggc cgtcgtttta
     6421 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     6481 cctttcgcca gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     6541 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     6601 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     6661 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     6721 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     6781 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     6841 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     6901 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     6961 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     7021 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     7081 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     7141 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     7201 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     7261 acgttaaggg attttggtca tcgaacccca gagtcccggc tagatcacgc aactggtcca
     7321 gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg
     7381 actgtttttt tgggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt
     7441 gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa
     7501 aacaaagtta aacatcatga ggaggtaacg tcatggagaa aaaaatcact ggatatacca
     7561 ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc
     7621 aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga
     7681 aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc
     7741 atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc
     7801 cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc
     7861 acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa
     7921 acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct
     7981 gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg
     8041 ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc
     8101 aggttcatca tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac
     8161 agtactgcga tgagtggcag ggcggggcgt aacctaggca tgatgatata tttttatctt
     8221 gtgcaatgta acatcagaga ttttgagaca caacgtggct ttcccc
//
