LOCUS       sequence-310722-        6460 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Short Fibronectin FRET standard.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6460)
  AUTHORS   Duvall SW, Childers WS
  TITLE     Design of a Histidine Kinase FRET Sensor to Detect Complex Signal 
            Integration within Living Bacteria.
  JOURNAL   ACS Sens. 2020 Jun 26;5(6):1589-1596. doi: 
            10.1021/acssensors.0c00008. Epub 2020 Jun 16.
  PUBMED    32495620
REFERENCE   2  (bases 1 to 6460)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssensors.0c00008"; journalName: "ACS Sens"; date: 
            "2020-06-26- 26"; volume: "5"; issue: "6"; pages: "1589-1596"
COMMENT     Sequence Label: pSWD55
FEATURES             Location/Qualifiers
     source          1..6460
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            21..130
                     /note="incP origin of transfer"
     primer_bind     282..298
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      299..345
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(2887..2908)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     3495..3516
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3841..4545
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby2, with improved brightness and photostability (Bajar 
                     et al., 2016)"
                     /label=mRuby3
                     /translation="SKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTMRI
                     KVIEGGPLPFAFDILATSFMYGSRTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGGVV
                     TVTQDTSLEDGELVYNVKVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTDIA
                     LKVDGGGHLHCNFVTTYRSKKTVGNIKMPGVHAVDHRLERIEESDNETYVVQREVAVAK
                     YSNLGGGMDELYK"
     primer_bind     complement(4564..4580)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4591..4608)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4591..4607)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4600..4622)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(4805..5393)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4885..4904)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             5720..6379
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(5786..5805)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ccaccgcggt ggcggccgcc ccggccagcc tcgcagagca ggattcccgt tgagcaccgc
       61 caggtgcgaa taagggacag tgaagaagga acacccgctc gcgggtgggc ctacttcacc
      121 tatcctgccc ggctgacgcc gttggataca ccaaggaaag tctacacgaa ccctttggca
      181 aaatcctgta tatcgtgcga aaaaggatgg atataccgaa aaaatcgcta taatgacccc
      241 gaagcagggt tatgcagcgg aaaagatccg tcgacggccg ctctagaact agtggatcca
      301 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcgca
      361 ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtttg
      421 tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtttttat ataagcttag
      481 cgctaccgga ctcagatcca gatcgaaatg taccggctga actggtggaa gcacgttgag
      541 cctcgtcaag gactcatgaa gcgcgtccct gctctcagtc gcctgctcgt tacgcctgag
      601 gtgtcaaaac accgtctgtt catttggcta gacgctagag ttcttccgga tcacaagctc
      661 caggtcgtta cactcgatga cgactgctca ttcggagttt tacactcaag gttccacgag
      721 gtgtgggctc ttgcagcggg gtcgtggcac ggctcaggca atgatccgcg ctacaccatc
      781 tccaccacct tcgagacctt ccccttcccg gaaggcctga cgcccaacat agccgccgtc
      841 gactatgagg gcgacccgcg cgcccaggcc atcgccgcgg cggcggccga gctgaaccgg
      901 ctgcgggaag cctggctcaa tccgcccgac ctggtgcgga tcgagcccga ggtcgtgccc
      961 ggctatcccg accgcgtgct gcccgtcagc cccgaggcgg gcgccgagct gaagaagcgc
     1021 accctgacca acctctacaa ccagcgcccg gcctggctgg acatggccca ccagcgcctg
     1081 gacgccgccg tcgccgccgc ctacggctgg cccgacggcc tgaccgacga cgagatcctg
     1141 gagcgcctgt tcgccctcaa ccaggagcgc gcggcggcgg ggcggtaggt tagacccctc
     1201 caaaaccccg acctccccgg cgaaggccgg ggtccagtta catccggaac gcttggagtg
     1261 ggcccgccta cggcctcgac caacccgcag tcggcaaggg gttcgatctg gaccccggca
     1321 ttcgccgggg aggtcggcag agaggtcaga accgcgtctt gcagccctgc ttcagatgaa
     1381 ccccgcgcag gaagccgcgg cgggccaggc cggcgtcgac ggcggcgttc atctggcggt
     1441 cggcgggggc agcgccggtg acttcgcgca ccaggccccg gaacgggatg atgctgttga
     1501 tcagggtctt gccaccggcc tcggccagct tgccggctcg gttttcggcg tactcggcgc
     1561 cgaccttgta gtcgggcccc agcgccgcgt tcagcgcctt cacctcattg gccagggcct
     1621 ggcaggactt cagacccgcg aggctgtagg ggtcgtcaaa ggccttggcc aggaccggcg
     1681 gcacctcgcg cttggacagg ccgacgtcgc gcaccggctg ggtgccgatg tcgacggcct
     1741 tcttgcccgc ctcgccccag cggccggacg gttcctcggg cttggccggc ggcttgacct
     1801 gggcgtgggc gctggaggcc agcagcacgg cggcggctcc ggccatgatc agcagcttgt
     1861 tcggcatcaa aagcgccctc ccgacaaaac acgccacggg aacggcaagc ctcgccgccg
     1921 gttcccgccc gcaagtccct cggcacgaac tctaaggcct gcgcacgatc catcgccacc
     1981 acaaagcgtg tcatcccggc cgaagcgcag cggagagccg ggacccaggg ccacaagcac
     2041 ggcgcttcgt caccccctgg gtcccggata gcgctccgcg cttccgggat gacacgggtt
     2101 taagcacttg ggtgagaagc cctagttcgg cgcgccgccc gcttccaggt cgaacgcggc
     2161 gtcgaaggcg tcgtcgccag gaaccgtccc ttccagggcg gcgtcgaaca ggtcttcgag
     2221 cgatgaccag atgaaggcca tctgctccat gatcgtcggg acgctggccg gatcgaagcc
     2281 ggggtagagg tcttccacca gccacaggcc cgtgccggga tcgaaggcga agcggcagcc
     2341 gatcaggcgg aactgggcgc gggccatggt ctcgaacagg gccgtcaggt cgcgggcggc
     2401 gtccaggtcg tcgtggtcca gcaccaggca gcggatctgc agccagccgt ggtcgggcag
     2461 caggtagaag gcgccctcgt cctgatcctc gcccgaaacc tccagccccc ggtcgatggc
     2521 ttcgacgaca tagccggccg cgcggcaggt gtcggtgagc gcggccagca gggcggcttc
     2581 ctggtcaggg gtcaggtcgg tcatgggcaa gaggtccagg tcgtggtttg tcggcggctt
     2641 ctagcatgga ccgcccgcgc ccgtgaggcc gaggatttcg cgctggtcag acaacctact
     2701 tgccgtcccc acatgttagc gctaccaagt gccgacgaac gcgcgccgcc gacggtgtcg
     2761 gcgcttcaga cgctcgagtt ttggggagac gaccatatgc ctgcaggcgc cttaattaat
     2821 atgcatggta ccttaagatc tcgaatgagc aagggcgagg agctgttcac cggggtggtg
     2881 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt ccgcggcgag
     2941 ggcgagggcg atgccaccaa cggcaagctg accctgaagt tcatctgcac caccggcaag
     3001 ctgcccgtgc cctggcccac cctcgtgacc accttcggct acggcgtggc ctgcttcagc
     3061 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     3121 gtccaggagc gcaccatctc tttcaaggac gacggtacct acaagacccg cgccgaggtg
     3181 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     3241 gacggcaaca tcctggggca caagctggag tacaacttca acagccacta cgtctatatc
     3301 acggccgaca agcagaagaa ctgcatcaag gctaacttca agatccgcca caacgttgag
     3361 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     3421 gtgctgctgc ccgacaacca ctacctgagc catcagtcca agctgagcaa agaccccaac
     3481 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccctgga aagcggcggc
     3541 gaagatccga tggtgagcgt gtcggacgtc ccgcgcgatc tcgaagtggt cgcggcgacg
     3601 ccgacgtcgc tgctgatctc gtgggatgcc cccgccgtca ccgtgcggta ttatcgcatc
     3661 acgtatgggg aaaccggcgg caactcgccg gtgcaggagt ttaccgtgcc gggctccaag
     3721 tccaccgcga cgatctccgg gctcaagccg ggcgtggact acaccatcac cgtgtacgcc
     3781 gtgaccgggc ggggggactc ccccgcgtcc tcgaagccga tctccatcaa ctatcgcacc
     3841 tctaagggcg aagagctgat caaggaaaat atgcgtatga aggtggtcat ggaaggttcg
     3901 gtcaacggcc accaattcaa atgcacaggt gaaggagaag gcagaccgta cgagggagtg
     3961 caaaccatga ggatcaaagt catcgaggga ggacccctgc catttgcctt tgacattctt
     4021 gccacgtcgt tcatgtatgg cagccgtacc tttatcaagt acccggccga catccctgat
     4081 ttctttaaac agtcctttcc tgagggtttt acttgggaaa gagttacgag atacgaagat
     4141 ggtggagtcg tcaccgtcac gcaggacacc agccttgagg atggcgagct cgtctacaac
     4201 gtcaaggtca gaggggtaaa ctttccctcc aatggtcccg tgatgcagaa gaagaccaag
     4261 ggttgggagc ctaatacaga gatgatgtat ccagcagatg gtggtctgag aggatacact
     4321 gacatcgcac tgaaagttga tggtggtggc catctgcact gcaacttcgt gacaacttac
     4381 aggtcaaaaa agaccgtcgg gaacatcaag atgcccggtg tccatgccgt tgatcaccgc
     4441 ctggaaagga tcgaggagag tgacaatgaa acctacgtag tgcaaagaga agtggcagtt
     4501 gccaaataca gcaaccttgg tggtggcatg gacgagctgt acaagtaaag ctgcagcccg
     4561 ggggatccac tagttctaga gcggccattc actggccgtc gttttacaac gtcgtgactg
     4621 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagcgg
     4681 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     4741 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     4801 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     4861 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     4921 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     4981 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     5041 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     5101 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     5161 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     5221 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     5281 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     5341 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     5401 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     5461 tggtcatcga accccagagt cccggctaga tcacgcaact ggtccagaac cttgaccgaa
     5521 cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttggg
     5581 gtaccgtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg
     5641 atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac
     5701 atcatgagga ggtaacgtca tggagaaaaa aatcactgga tataccaccg ttgatatatc
     5761 ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa
     5821 ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa
     5881 gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaattccg
     5941 tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt
     6001 tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg
     6061 gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt
     6121 ccctaaaggg tttattgaga atatgttttt cgtctcagcc aatccctggg tgagtttcac
     6181 cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg
     6241 caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc
     6301 cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga
     6361 gtggcagggc ggggcgtaac ctaggcatga tgatatattt ttatcttgtg caatgtaaca
     6421 tcagagattt tgagacacaa cgtggctttc cccccccccc
//
