LOCUS       sequence-310751-        5379 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry clone for Golden Gate assembly. Golden gate compatible N. 
            benthamiana ZAR1 without STOP codon .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5379)
  AUTHORS   Harant A, Pai H, Sakai T, Kamoun S, Adachi H
  TITLE     A vector system for fast-forward studies of the HOPZ-ACTIVATED 
            RESISTANCE1 (ZAR1) resistosome in the model plant Nicotiana 
            benthamiana.
  JOURNAL   Plant Physiol. 2022 Jan 20;188(1):70-80. doi: 
            10.1093/plphys/kiab471.
  PUBMED    34633454
REFERENCE   2  (bases 1 to 5379)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Plant Physiol"; date: "2022-01-20- 20"; volume: "188"; issue: "1"; 
            pages: "70-80"
COMMENT     Sequence Label: pZA13
FEATURES             Location/Qualifiers
     source          1..5379
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1592..1603
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(3258..3357)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3374..3393)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3375..3393)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3398..3414)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3398..3414)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3845..4636
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4729..5317
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5218..5237
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc gaattcgccc ttaatggtct ctaatggtgg atgcggtggt cactgtattc
      721 ttggagaaac ttctgcatgt tctcacagag gagagtaggt ttcttactaa atacagacag
      781 cagtttgaaa aactgaagaa cgagctgcta ttcatgcaaa gctttctcaa agatgcagag
      841 aggctcaaga ggaaaaacaa cacccttaaa ggagtcatgt cctgtttgcg agacttaatc
      901 tttgaagctg aagagatact tgaggactgc cagaatcaat ctgcagatag cgatcgggct
      961 actacatgct tccatcctaa aaggctctct cttcgccatc aaactgggaa gtgccttgct
     1021 aaaatcaatg acagaatctc ggagataaag caaaacattt caacatatct tggagtacca
     1081 cttctcgaag aaggaagtat ggaggcacac aataatctaa tgtcaagatg gacttcttcc
     1141 ctttatgacc acactcaggt agttggtttg gaaggtgaca ctgagaagat aaaggattgg
     1201 ctatttgaag caagagatgg tttacttacc attgcatttg tgggtatggg aggacttggt
     1261 aaaaccactc ttgctcagaa agttttcaat gacaaaaggg tggaggatca cttggagagg
     1321 aggatttggg tgtctgtttc tcaaacattt actgaggaac aagtcatgag aagcatattg
     1381 aggagtttag gagatgcatg tgttggtgat gaccagtgtg aactgttaag aaaaataaac
     1441 cagtaccttt tgggaaagag gtttttgatt gttatggatg atgtttggag ctgggacaat
     1501 gcctggtggc aaaaaatcta tactggactg cccaaaggaa atgggagcac tgttattgta
     1561 acaacgagaa atgagctagt cgctcgcaag atgggagtta cagaagcaag aatacattgg
     1621 ccaaaattcc tcaatgagca ctacagttgg ttactattcc ggaagattgc atttgcagga
     1681 agtgcaggtg aatgccattt ccccgagttg gaggacgtgg gaaaggagat tgtggaaaaa
     1741 tgcaagggac ttcctttagc aatcaaagca gtaggaggag tgatgctttg caagccatct
     1801 tactatcacg aatggaggcg tatttcaaat catttccgtg atgaattgaa agaaaatgat
     1861 gactcagtga tggcctcact acagttgagc tatgatgaac tccctcctta cttgaaatcc
     1921 tgtttcctct gtttttcact ctttcctgag gattgtgtca taccaaaaga ccaactgatc
     1981 cgttggtgga ttggagaagg tttcatacct ctgagaagtg gtaggttatc gactgaagtg
     2041 ggagaagatt gtttctcaca actatccaat cggtgtttga tagaagttgt tgataaggct
     2101 tacaatggtg tgatccacac atgcaaaatg catgacatgg ttcgtgactt ggtgatcaaa
     2161 ttagccgagg atgatgcatt tttcacccca gctgatgcaa cttgtcggca tttgggtatt
     2221 aagagtgaga tgaattggaa gcaactattg agcaatcaaa aattacgagc actgctaaca
     2281 accaccaaga gtggtgaagt taacaaaatc cattccgaca ttgccaagaa gctgtgcaag
     2341 agtcgacacc tccaagtatt ggatctgtca aaatcaattt tcgatgtgcc tctttcaagt
     2401 ttgttggaag gaattggatc tgctaagcag ctcacttatc tcagtttaag caatacacat
     2461 ccaatgattg gagttccagc ttccatatcc aagcttgaaa aattacagat tttggatttc
     2521 agctattgcc aaaatatgaa aatgctcccc tcttgcgttc tgacatttga ggaacttgct
     2581 gttttggatg tgaacaactg tggttccctt gagtaccttc caaaaggatt aagtaggctt
     2641 tccaatcttc aagtattgct tgggtttaag cctgcaaaat taagccaacc aggaggttgt
     2701 cgtattgctg agctcagaag cctgactcga ctcagaactc tcagtttaag actaaccgaa
     2761 aatgaggaga ttggagacga tgaggggaac gcactggtag atctccaaga acttcaattt
     2821 ttaacaataa gttgctttgg cagtcaagat aatggacttg cgacaaaact tggtagactt
     2881 tatcctccac gacaactcca cgagctgatt ctcaaatttt atccaagtaa gacaagtcct
     2941 gaatggctta accccaatct ctcccccatg ttgcgatatt tatcaatcat ctctggtgat
     3001 attacacaaa tgcacgagaa cttctggggt gatggtagta ctgcttggaa aattgagggc
     3061 ttaatgttgg aatctttatc ggatttgaga ttggaatggt cagcaatgca tcaagtcatg
     3121 ccttcgttaa gaatactcaa ggttagctgg tgccctgagt tggagtcatt tccaattgaa
     3181 gatgcagggt ttagaggggg tctatggaag aaggaagaac ataggaacgg ttcgagagac
     3241 cattaagggc gaattcgacc cagctttctt gtacaaagtt ggcattataa aaaataattg
     3301 ctcatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca
     3361 tccagctgat atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct
     3421 ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg
     3481 cctcctctag accagccagg acagaaatgc ctcgacttcg ctgctaccca aggttgccgg
     3541 gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag tggacataag cctgttcggt
     3601 tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga
     3661 acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact gtttttttgg
     3721 ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt
     3781 gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttaaa
     3841 cattatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt
     3901 catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga
     3961 tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg taaggcttga
     4021 tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga
     4081 gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg
     4141 gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc
     4201 aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag
     4261 agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga
     4321 acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg
     4381 ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac
     4441 cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca
     4501 gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt
     4561 ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg agatcaccaa
     4621 ggtagtcggc aaataaccct cgagccaccc atgaccaaaa tcccttaacg tgagttacgc
     4681 gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     4741 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     4801 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     4861 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     4921 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     4981 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     5041 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     5101 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     5161 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     5221 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     5281 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     5341 acggttcctg gccttttgct ggccttttgc tcacatgtt
//
