LOCUS       sequence-310761-       12556 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Chromosomal integration of PgpdA-dLbCas12a-VPR-TtrpC. Contains 1kb 
            homology arms for Aspergillus nidulans and the bar gene for 
            glufosinate selection. Filamentous fungi integrative vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12556)
  AUTHORS   Roux I, Woodcraft C, Hu J, Wolters R, Gilchrist CLM, Chooi YH
  TITLE     CRISPR-mediated activation of biosynthetic gene clusters for 
            bioactive molecule discovery in filamentous fungi.
  JOURNAL   ACS Synth Biol. 2020 Jun 11. doi: 10.1021/acssynbio.0c00197.
  PUBMED    32526136
REFERENCE   2  (bases 1 to 12556)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2020 Jun 11. doi: 10.1021/acssynbio.0c00197."
COMMENT     Sequence Label: pCRI001-pGEM-LS-Bar-PgpdA-dLbCas12a-VPR-Ttrpc-LS
FEATURES             Location/Qualifiers
     source          1..12556
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1..552)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(575..934)
                     /label=trpC promoter
                     /note="promoter for Aspergillus nidulans trpC"
     CDS             1665..5348
                     /codon_start=1
                     /product="CRISPR-associated protein from Lachnospiraceae 
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /label=LbCpf1
                     /translation="MSKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAE
                     DYKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKE
                     IAKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFS
                     EEAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEG
                     EFFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVL
                     SDRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKN
                     GPAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQ
                     LQEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKD
                     LLDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYS
                     KDKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNG
                     NYEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKL
                     IDFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLV
                     EEGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKK
                     EELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKI
                     NTEVRVLLKHDDNPYVIGIARGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTD
                     YHSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSG
                     FKNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQ
                     NGFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALD
                     YKNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQ
                     QGDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRN
                     YEAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTS
                     VKH"
     CDS             5349..5396
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5403..5483
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             5490..5510
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5535..5684
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             5709..5729
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6159..6515
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     CDS             6534..7103
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     regulatory      8485..8494
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      8489..8498
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(8528..8547)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8529..8547)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8554..8571)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8554..8570)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8563..8585)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8711..9166
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8798..8817)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9008..9029
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9244..9348
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9349..9417,9418..10209)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9567..9586)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10380..10968
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10869..10888
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11122..11139
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11256..11277
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11292..11297,11298..11315,11316..11322)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11330..11346
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11335..11357
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11354..11370
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11354..11370
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        11388..11406
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     11388..11405
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 ctaaatctcg gtgacgggca ggaccggacg gggcggtacc ggcaggctga agtccagctg
       61 ccagaaaccc acgtcatgcc agttcccgtg cttgaagccg gccgcccgca gcatgccgcg
      121 gggggcatat ccgagcgcct cgtgcatgcg cacgctcggg tcgttgggca gcccgatgac
      181 agcgaccacg ctcttgaagc cctgtgcctc cagggacttc agcaggtggg tgtagagcgt
      241 ggagcccagt cccgtccgct ggtggcgggg ggagacgtac acggtcgact cggccgtcca
      301 gtcgtaggcg ttgcgtgcct tccaggggcc cgcgtaggcg atgccggcga cctcgccgtc
      361 cacctcggcg acgagccagg gatagcgctc ccgcagacgg acgaggtcgt ccgtccactc
      421 ctgcggttcc tgcggctcgg tacggaagtt gaccgtgctt gtctcgatgt agtggttgac
      481 gatggtgcag accgccggca tgtccgcctc ggtggcacgg cggatgtcgg ccgggcgtcg
      541 ttctgggctc atggttactt cctaatcgaa gctttgcttg ggtagaatag gtaagtcaga
      601 ttgaatctga aataaaggga ggaagggcga acttaagaag gtatgaccgg gtcgttcact
      661 taccttgctt gacaaacgca ccaagttatc gtgcaccaag cagcagatga taataatgtc
      721 ctcgttcctg tctgctaata agagtcacac ttcgagcgcc gccgctactg ctacaagtgg
      781 ggctgatctg accagttgcc taaatgaacc atcttgtcaa acgacacaaa ttttgtgctc
      841 accgcctgga cgactaaacc aaaataggca ttcattgttg acctccacta gctccagcca
      901 agcccaaaaa gtgctccttc aatatcatct tctgtcgacc atgcggagag acggacggac
      961 gcagagagaa gggctgagta ataagcgcca ctgcgccaga cagctctggc ggctctgagg
     1021 tgcagtggat gattattaat ccgggaccgg ccgcccctcc gccccgaagt ggaaaggctg
     1081 gtgtgcccct cgttgaccaa gaatctattg catcatcgga gaatatggag cttcatcgaa
     1141 tcaccggcag taagcgaagg agaatgtgaa gccaggggtg tatagccgtc ggcgaaatag
     1201 catgccatta acctaggtac agaagtccaa ttgcttccga tctggtaaaa gattcacgag
     1261 atagtacctt ctccgaagta ggtagagcga gtacccggcg cgtaagctcc ctaattggcc
     1321 catccggcat ctgtagggcg tccaaatatc gtgcctctcc tgctttgccc ggtgtatgaa
     1381 accggaaagg ccgctcagga gctggccagc ggcgcagacc gggaacacaa gctggcagtc
     1441 gacccatccg gtgctctgca ctcgacctgc tgaggtccct cagtccctgg taggcagctt
     1501 tgccccgtct gtccgcccgg tgtgtcggcg gggttgacaa ggtcgttgcg tcagtccaac
     1561 atttgttgcc atattttcct gctctcccca ccagctgctc ttttcttttc tctttctttt
     1621 cccatcttca gtatattcat cttcccatcc aagaaccttt aatcatgagc aagctggaga
     1681 agtttacaaa ctgctactcc ctgtctaaga ccctgaggtt caaggccatc cctgtgggca
     1741 agacccagga gaacatcgac aataagcggc tgctggtgga ggacgagaag agagccgagg
     1801 attataaggg cgtgaagaag ctgctggatc gctactatct gtcttttatc aacgacgtgc
     1861 tgcacagcat caagctgaag aatctgaaca attacatcag cctgttccgg aagaaaacca
     1921 gaaccgagaa ggagaataag gagctggaga acctggagat caatctgcgg aaggagatcg
     1981 ccaaggcctt caagggcaac gagggctaca agtccctgtt taagaaggat atcatcgaga
     2041 caatcctgcc agagttcctg gacgataagg acgagatcgc cctggtgaac agcttcaatg
     2101 gctttaccac agccttcacc ggcttctttg ataacagaga gaatatgttt tccgaggagg
     2161 ccaagagcac atccatcgcc ttcaggtgta tcaacgagaa tctgacccgc tacatctcta
     2221 atatggacat cttcgagaag gtggacgcca tctttgataa gcacgaggtg caggagatca
     2281 aggagaagat cctgaacagc gactatgatg tggaggattt ctttgagggc gagttcttta
     2341 actttgtgct gacacaggag ggcatcgacg tgtataacgc catcatcggc ggcttcgtga
     2401 ccgagagcgg cgagaagatc aagggcctga acgagtacat caacctgtat aatcagaaaa
     2461 ccaagcagaa gctgcctaag tttaagccac tgtataagca ggtgctgagc gatcgggagt
     2521 ctctgagctt ctacggcgag ggctatacat ccgatgagga ggtgctggag gtgtttagaa
     2581 acaccctgaa caagaacagc gagatcttca gctccatcaa gaagctggag aagctgttca
     2641 agaattttga cgagtactct agcgccggca tctttgtgaa gaacggcccc gccatcagca
     2701 caatctccaa ggatatcttc ggcgagtgga acgtgatccg ggacaagtgg aatgccgagt
     2761 atgacgatat ccacctgaag aagaaggccg tggtgaccga gaagtacgag gacgatcgga
     2821 gaaagtcctt caagaagatc ggctcctttt ctctggagca gctgcaggag tacgccgacg
     2881 ccgatctgtc tgtggtggag aagctgaagg agatcatcat ccagaaggtg gatgagatct
     2941 acaaggtgta tggctcctct gagaagctgt tcgacgccga ttttgtgctg gagaagagcc
     3001 tgaagaagaa cgacgccgtg gtggccatca tgaaggacct gctggattct gtgaagagct
     3061 tcgagaatta catcaaggcc ttctttggcg agggcaagga gacaaacagg gacgagtcct
     3121 tctatggcga ttttgtgctg gcctacgaca tcctgctgaa ggtggaccac atctacgatg
     3181 ccatccgcaa ttatgtgacc cagaagccct actctaagga taagttcaag ctgtattttc
     3241 agaaccctca gttcatgggc ggctgggaca aggataagga gacagactat cgggccacca
     3301 tcctgagata cggctccaag tactatctgg ccatcatgga taagaagtac gccaagtgcc
     3361 tgcagaagat cgacaaggac gatgtgaacg gcaattacga gaagatcaac tataagctgc
     3421 tgcccggccc taataagatg ctgccaaagg tgttcttttc taagaagtgg atggcctact
     3481 ataaccccag cgaggacatc cagaagatct acaagaatgg cacattcaag aagggcgata
     3541 tgtttaacct gaatgactgt cacaagctga tcgacttctt taaggatagc atctcccggt
     3601 atccaaagtg gtccaatgcc tacgatttca acttttctga gacagagaag tataaggaca
     3661 tcgccggctt ttacagagag gtggaggagc agggctataa ggtgagcttc gagtctgcca
     3721 gcaagaagga ggtggataag ctggtggagg agggcaagct gtatatgttc cagatctata
     3781 acaaggactt ttccgataag tctcacggca cacccaatct gcacaccatg tacttcaagc
     3841 tgctgtttga cgagaacaat cacggacaga tcaggctgag cggaggagca gagctgttca
     3901 tgaggcgcgc ctccctgaag aaggaggagc tggtggtgca cccagccaac tcccctatcg
     3961 ccaacaagaa tccagataat cccaagaaaa ccacaaccct gtcctacgac gtgtataagg
     4021 ataagaggtt ttctgaggac cagtacgagc tgcacatccc aatcgccatc aataagtgcc
     4081 ccaagaacat cttcaagatc aatacagagg tgcgcgtgct gctgaagcac gacgataacc
     4141 cctatgtgat cggcatcgct cgaggcgagc gcaatctgct gtatatcgtg gtggtggacg
     4201 gcaagggcaa catcgtggag cagtattccc tgaacgagat catcaacaac ttcaacggca
     4261 tcaggatcaa gacagattac cactctctgc tggacaagaa ggagaaggag aggttcgagg
     4321 cccgccagaa ctggacctcc atcgagaata tcaaggagct gaaggccggc tatatctctc
     4381 aggtggtgca caagatctgc gagctggtgg agaagtacga tgccgtgatc gccctggagg
     4441 acctgaactc tggctttaag aatagccgcg tgaaggtgga gaagcaggtg tatcagaagt
     4501 tcgagaagat gctgatcgat aagctgaact acatggtgga caagaagtct aatccttgtg
     4561 caacaggcgg cgccctgaag ggctatcaga tcaccaataa gttcgagagc tttaagtcca
     4621 tgtctaccca gaacggcttc atcttttaca tccctgcctg gctgacatcc aagatcgatc
     4681 catctaccgg ctttgtgaac ctgctgaaaa ccaagtatac cagcatcgcc gattccaaga
     4741 agttcatcag ctcctttgac aggatcatgt acgtgcccga ggaggatctg ttcgagtttg
     4801 ccctggacta taagaacttc tctcgcacag acgccgatta catcaagaag tggaagctgt
     4861 actcctacgg caaccggatc agaatcttcc ggaatcctaa gaagaacaac gtgttcgact
     4921 gggaggaggt gtgcctgacc agcgcctata aggagctgtt caacaagtac ggcatcaatt
     4981 atcagcaggg cgatatcaga gccctgctgt gcgagcagtc cgacaaggcc ttctactcta
     5041 gctttatggc cctgatgagc ctgatgctgc agatgcggaa cagcatcaca ggccgcaccg
     5101 acgtggattt tctgatcagc cctgtgaaga actccgacgg catcttctac gatagccgga
     5161 actatgaggc ccaggagaat gccatcctgc caaagaacgc cgacgccaat ggcgcctata
     5221 acatcgccag aaaggtgctg tgggccatcg gccagttcaa gaaggccgag gacgagaagc
     5281 tggataaggt gaagatcgcc atctctaaca aggagtggct ggagtacgcc cagaccagcg
     5341 tgaagcacaa aaggccggcg gccacgaaaa aggccggcca ggcaaaaaag aaaaagggat
     5401 cctacccata cgatgttcca gattacgctt atccctacga cgtgcctgat tatgcatacc
     5461 catatgatgt ccccgactat gccgctgacc ccaagaagaa gaggaaggtg gaggccagcg
     5521 gttccggacg ggctgacgca ttggacgatt ttgatctgga tatgctggga agtgacgccc
     5581 tcgatgattt tgaccttgac atgcttggtt cggatgccct tgatgacttt gacctcgaca
     5641 tgctcggcag tgacgccctt gatgatttcg acctggacat gctgattaac tctagaagtt
     5701 ccggatctcc gaaaaagaaa cgcaaagttg gtagccagta cctgcccgac accgacgacc
     5761 ggcaccggat cgaggaaaag cggaagcgga cctacgagac attcaagagc atcatgaaga
     5821 agtccccctt cagcggcccc accgacccta gacctccacc tagaagaatc gccgtgccca
     5881 gcagatccag cgccagcgtg ccaaaacctg ccccccagcc ttaccccttc accagcagcc
     5941 tgagcaccat caactacgac gagttcccta ccatggtgtt ccccagcggc cagatctctc
     6001 aggcctctgc tctggctcca gcccctcctc aggtgctgcc tcaggctcct gctcctgcac
     6061 cagctccagc catggtgtct gcactggctc aggcaccagc acccgtgcct gtgctggctc
     6121 ctggacctcc acaggctgtg gctccaccag cccctaaacc tacacaggcc ggcgagggca
     6181 cactgtctga agctctgctg cagctgcagt tcgacgacga ggatctggga gccctgctgg
     6241 gaaacagcac cgatcctgcc gtgttcaccg acctggccag cgtggacaac agcgagttcc
     6301 agcagctgct gaaccagggc atccctgtgg cccctcacac caccgagccc atgctgatgg
     6361 aataccccga ggccatcacc cggctcgtga caggcgctca gaggcctcct gatccagctc
     6421 ctgcccctct gggagcacca ggcctgccta atggactgct gtctggcgac gaggacttca
     6481 gctctatcgc cgacatggac ttctccgcac tgctgggtag cggatcggga tctcgggatt
     6541 ccagggaagg gatgtttttg ccgaagcctg aggccggctc cgctattagt gacgtgtttg
     6601 agggccgcga ggtgtgccag ccaaaacgaa tccggccatt tcatcctcca ggaagtccat
     6661 gggccaaccg cccactcccc gccagcctcg caccaacacc aaccggtcca gtacatgagc
     6721 cagtcgggtc actgaccccg gcaccagtcc ctcagccact ggatccagcg cccgcagtga
     6781 ctcccgaggc cagtcacctg ttggaggatc ccgatgaaga gacgagccag gctgtcaaag
     6841 cccttcggga gatggccgat actgtgattc cccagaagga agaggctgca atctgtggcc
     6901 aaatggacct ttcccatccg cccccaaggg gccatctgga tgagctgaca accacacttg
     6961 agtccatgac cgaggatctg aacctggact cacccctgac cccggaattg aacgagattc
     7021 tggatacctt cctgaacgac gagtgcctct tgcatgccat gcatatcagc acaggactgt
     7081 ccatcttcga cacatctctg ttttagtggg cgacgacgac gttcgagggg gggcccggta
     7141 ccgccccgtc cggtcctgcc cgtcaccgag atccacttaa cgttactgaa atcatcaaac
     7201 agcttgacga atctggatat aagatcgttg gtgtcgatgt cagctccgga gttgagacaa
     7261 atggtgttca ggatctcgat aagatacgtt catttgtcca agcagcaaag agtgccttct
     7321 agtgatttaa tagctccatg tcaacaagaa taaaacgcgt ttcgggttta cctcttccag
     7381 atacagctca tctgcaatgc atggacgtaa actcctcttc agaccaaagg ttgggtcgtc
     7441 ggtaggggaa tatccttggt tggggccctg gtcataaatg ccgtattacc gcagtcagat
     7501 gttgggctgt cagtgggcta gaagcaggga tgctctggtc ccagtcccag gtataaccag
     7561 ctttcttagt gtatatatcg aaagcagcag ctatcttacg ccttgtctgg gtttggctgg
     7621 cttagttcac aatgtcctca actcatctgg tgactacgta ttgttctcac tttggaggcg
     7681 gttcatcgca cgtaagagcc tttggatcga aagtcaattc gcaaatcagc caaaataagc
     7741 atcacgctaa caaccggcca gtgaatgctc ctgggcaatg acgagctcat ataagaccgg
     7801 tagtccattt cagtccaatg acgagtcatc gcaatacttg acccgcacat ccacatttca
     7861 caaggatgta agggagccct aagatagtca ccgaacgggc tagttgctgt gaggatcctg
     7921 gtgaagttca agttaatgac ataggaaaat catctttatt ccagggagaa gaataggcca
     7981 atcgtttacc ctttgctcgt tctttctgat ggcctgtcta tctaccaaaa agtttgtcta
     8041 ggatataatc taggcaaatg aagcaagaat caataccatc ctttctgccc tatggtgtag
     8101 acttagaaag tggatgcact gtctgccaca gttatgcctt ggtcaggcgc tgtgacgtaa
     8161 tggctcgcag ccacaatttt gaatcgacag ccaattccag ttcaagcccc gataccctgt
     8221 ttatgtttac caagacaagc atctattatc agatactacc aggaaattct aagagcgcga
     8281 ccggcccctg atcggaggtt ggttgtgtgt ttttgctgaa ctgggggact cgcacaccaa
     8341 ccggacgaat tctgcacact gataccacac tcgcttcatg caagtttcaa gccatccctt
     8401 aacgcgtcag tgcatacaaa tgagtgtcta tcgacccatt aacgcgctgt ccaatgctgg
     8461 gcgacgacga cgtgaattcc cgcggccgcc atggcggccg ggagcatgcg acgtcgggcc
     8521 caattcgccc tatagtgagt cgtattacaa ttcactggcc gtcgttttac aacgtcgtga
     8581 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     8641 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     8701 tggcgaatgg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     8761 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     8821 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     8881 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     8941 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     9001 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     9061 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     9121 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttcctg atgcggtatt
     9181 ttctccttac gcatctgtgc ggtatttcac accgcatcag gtggcacttt tcggggaaat
     9241 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     9301 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     9361 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     9421 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     9481 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     9541 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     9601 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     9661 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     9721 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     9781 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     9841 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     9901 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     9961 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
    10021 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
    10081 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
    10141 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
    10201 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
    10261 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
    10321 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
    10381 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
    10441 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
    10501 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
    10561 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
    10621 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
    10681 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
    10741 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
    10801 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
    10861 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
    10921 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
    10981 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
    11041 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
    11101 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
    11161 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
    11221 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
    11281 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
    11341 taacaatttc acacaggaaa cagctatgac catgattacg ccaagctatt taggtgacac
    11401 tatagaatac tcaagctatg catccaacgc gttgggagct ctcccatatg gtcgacctgc
    11461 aggcggccgc gaattcctcg tcgtgtcgcc caagtgaggt aatgattagg attgctggaa
    11521 agttacatag atataattat atatctctgc aatcttatgt tgcaatggtt tggtgtctgc
    11581 ctctgccagc gaggcattta cgttgtccat gaccgtatta aatataatac agcgcgccta
    11641 gcatgacttt tgatacttta aggatctctt ttaatgtctt cctgctgttg cctttttctg
    11701 gcctatgccc agaatgcgaa accgtcggca aatagatgaa actagctaag tgattggtca
    11761 tgtcactgtt aatgtttaga aaatggtatt gtacctggca tctcatcagc ctgttaacca
    11821 ggtaaggata ccgataatat gagaaggtat taataggaca agcatagtta gactaatgca
    11881 acgccaaatc ttgggtatcc tgtaagagac gttttcgggc aagcatctct cagcactttg
    11941 atcagatccc attctcgacc tgcctggatc gcaccgataa aactggcttg cggttataga
    12001 tgctctagtc aaaccatata gtcctaaata tacctgttga aatataacat aacaacgaaa
    12061 aatgccatga cagagcgtat ctagaaaggt atataccgtc ataatacagt caacagtcgc
    12121 ggatgcaggt tccgcgtact acttactacc acagcaaacc aatgccttca ttgcataccg
    12181 agcctgaacc attttataac ggacaggagc agtaacgcgc cggaacaggc ttggccaaga
    12241 gacgcaggaa ctcattgaga agcccaagtt ctcctagttt ggtcagacag tgtcctggca
    12301 cagcacgatt aattccccac ttacaaacga tagtctcggt atttcagacg tatatggatt
    12361 agttattttt tgtttgattt tcatggtcca gtaaatagcg atgaacgttg tctgagttct
    12421 acaaaaagtg cagttcacga tcagcaacgg caaacgacgc aatgcttcga ggtcctgtat
    12481 tgggtacgtg tcatcgtagc ttactaaatg acagcaggta gatcaaggtg ggacagggtt
    12541 attgtctcat gagcgg
//
