LOCUS       sequence-310764-       10493 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  human RNF160 (LTN1, ZNF294) FLAG mammalian expression vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10493)
  AUTHORS   Li S, Wang L, Berman M, Kong YY, Dorf ME
  TITLE     Mapping a dynamic innate immunity protein interaction network 
            regulating type I interferon production.
  JOURNAL   Immunity. 2011 Sep 23;35(3):426-40. Epub 2011 Sep 8.
  PUBMED    21903422
REFERENCE   2  (bases 1 to 10493)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Immunity.";
            date: "2011-09-23"; volume: "35(3)"; pages: "426-40. Epub 2011 Sep 
            8"
COMMENT     Sequence Label: pCMV3TAG8li-hRNF160-3FLAG
FEATURES             Location/Qualifiers
     source          1..10493
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        67..370
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        371..574
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     525..545
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        620..638
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             6001..6072
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     primer_bind     complement(6125..6144)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6126..6144)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6173..6190)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6179..6386
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(6393..6848)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6530..6551)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6742..6761
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6875..6979
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6980..7048,7049..7837)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7198..7217)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        7931..8076
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             8119..9156
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(9154..9173)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9198..9246
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      9797..10385
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10286..10305
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
       61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      541 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      601 gcgattacgc caagctcgaa attaaccctc actaaaggga acaaaagctg gctcgagctg
      661 aagcttcatg gatccaaagc ggccgcgacc atgggcggga agaacaagca gcgaactaaa
      721 gggaacctga ggccttcaaa cagtggccga gctgcagaac tccttgccaa agaacaggga
      781 acagtgcctg gatttattgg ttttggaaca tctcagagtg acctaggcta tgttcctgct
      841 attcaaggag ctgaagaaat tgacagtctt gtagattctg atttccgaat ggtgctgcgg
      901 aaactttcaa agaaagatgt caccacaaaa ttaaaagcta tgcaggaatt tggaaccatg
      961 tgtacagaga gagacacaga aactgtgaaa ggagttcttc catattggcc aagaattttt
     1021 tgcaaaattt cacttgatca tgaccgtcgc gtccgagaag ccacacaaca agcttttgaa
     1081 aaacttatcc ttaaagtaaa gaaacagttg gctccctact taaaaagttt aatgggatat
     1141 tggctaatgg ctcagtgtga tacttacaca ccagctgcgt ttgcagcaaa agatgcattt
     1201 gaagcggctt ttcctccaag caagcaacct gaagccatag cattttgtaa ggatgaaatt
     1261 acaagtgtgc tgcaggatca tcttataaaa gaaacacctg atacactcag tgacccgcaa
     1321 actgttccag aggaagaaag agaagctaaa ttctaccggg ttgtaacttg ttccttattg
     1381 gcattaaaga gattactttg ccttttacct gataatgagc ttgattctct ggaggagaaa
     1441 tttaagtctc ttttatcaca gaataagttt tggaagtatg gaaaacacag tgtacctcag
     1501 attcgctcag cttattttga gttagtctct gcattgtgcc agcgcattcc acagttgatg
     1561 aaagaggaag catccaaagt gagcccatca gttctactta gcattgatga cagtgaccca
     1621 attgtctgcc cagctctctg ggaagctgta ctctatacac ttacaactat tgaggactgt
     1681 tggcttcatg taaatgcaaa aaagagtgtg tttcccaagc tatcaactgt gattcgtgaa
     1741 ggtggtcggg gtctagctac tgtcatatat ccttaccttc tgccattcat cagcaagctc
     1801 cctcagtcca tcacaaatcc aaagttggat ttcttcaaaa atttcctcac gtctctagtt
     1861 gctgggctgt caacagagag aactaaaacc agctctttag agtcctcggc agtaatatct
     1921 gctttttttg aatgcttacg ttttataatg cagcaaaact taggtgagga agagattgaa
     1981 cagatgctcg tcaatgatca gttgatccct tttattgatg cagttctcaa agacccagga
     2041 ttgcaacatg ggcagctatt taaccattta gcagaaactc taagttcctg ggaagccaaa
     2101 gcagacacgg aaaaagatga aaaaacagct cacaacttgg agaacgtact gatacatttc
     2161 tgggaaagac tgtcagagat ctgtgttgcg aaaatcagtg agccagaagc tgatgttgag
     2221 tccgttttgg gtgtatctaa cctattacag gtgcttcaga agccgaagag ctcattgaag
     2281 tcaagtaaaa aaaaaaatgg taaggttaga tttgctgatg agatacttga aagcaataaa
     2341 gagaatgaaa aatgtgtatc ttcagaagga gagaagattg aaggctggga attaacaact
     2401 gaaccttctc tcactcataa ttcttcaggc cttttgtctc ctctaaggaa aaaacctttg
     2461 gaagacttag tctgtaaact cgcagatata agtattaatt atgtcaatga acgaaagtca
     2521 gagcaacatc taaggtttct ttctactctg cttgactcct tttcttcaag ccgagtattt
     2581 aaaatgctac ttggtgatga aaaacagagt attgtccaag ccaaacctct tgaaatagcc
     2641 aagcttgtac aaaaaaatcc tgcggtgcag tttttatacc agaaactgat aggttggcta
     2701 aatgaagatc aaaggaagga ttttggtttc ctggtggaca ttttgtacag tgctctccgg
     2761 tgctgtgaca atgatatgga aagaaaaaaa gtcttggatg atctaaccaa ggtggacttg
     2821 aaatggaatt ctcttcttaa gattattgaa aaggcatgtc ctagttcaga taaacatgct
     2881 ttagtaactc cttggctcaa aggcgatatc cttggtgaga aattggtcaa cttggcagat
     2941 tgtctttgta atgaggactt ggaatccagg gtatcttcag aatctcactt ctcagaaaga
     3001 tggactcttc taagcttggt attatcccaa catgttaaaa atgattactt gattggagac
     3061 gtatatgttg aaagaatcat tgttagactt catgaaactt tattcaaaac aaagaaatta
     3121 tcagaagctg aaagcagtga ctcatcagtg tcttttatct gtgatgtggc ctataactat
     3181 ttcagctcag cgaaaggatg cttgctaatg ccatcatctg aagatttatt attaactctc
     3241 tttcagttat gtgctcagag caaagaaaaa acacatttgc cagattttct tatctgtaaa
     3301 ctgaaaaata cttggctctc tggtgtaaat ttattggttc atcaaactga cagttcatat
     3361 aaagagagta ccttcctaca tttgtctgct ctgtggctga agaaccaagt tcaggcttca
     3421 tctttggata tcaacagtct ccaagtcctc ttgtctgctg ttgatgattt gctaaataca
     3481 cttctagaga gtgaagattc ttatcttatg ggagtttata ttggaagtgt aatgccgaac
     3541 gacagtgaat gggaaaagat gaggcagtct cttcctatgc agtggttaca tagacctctt
     3601 ttagagggaa gattgagttt gaattatgaa tgtttcaaaa cagattttaa ggaacaggac
     3661 ataaagacac ttcccagcca tttgtgtact tcagcattat tgagcaaaat ggtcttaatt
     3721 gcactgagaa aggaaacagt cttagaaaat aatgagcttg agaaaataat tgcagaactg
     3781 ctttattcac tgcagtggtg tgaagaatta gataacccac ctatttttct aattggattt
     3841 tgtgaaatac ttcaaaaaat gaatattacg tatgataact tacgtgtact tggtaatacg
     3901 tcgggccttt tgcagctgtt atttaacagg tccagagaac atggcacact gtggtctctt
     3961 attattgcta agttgatcct ttcccgaagc atttcatctg atgaagtaaa accacattat
     4021 aagagaaaag aaagtttttt tccactaact gaaggcaatt tgcataccat tcaaagtcta
     4081 tgtccatttt tgtcaaaaga agaaaagaaa gaatttagtg ctcaatgtat acctgctctt
     4141 ttgggctgga ctaagaaaga tctttgcagc actaatggag gttttggaca tcttgccatt
     4201 ttcaattctt gtctgcaaac caaaagtata gatgatggag agctattaca tggaatatta
     4261 aaaatcataa tatcctggaa gaaagagcat gaagatattt ttcttttcag ttgtaatcta
     4321 tcagaagcaa gtccagaggt actgggtgta aatatagaaa taatccggtt tctttcccta
     4381 tttctgaaat actgctcatc ccctttggca gagagtgagt gggacttcat catgtgctcc
     4441 atgttggctt ggttggagac aacaagtgag aatcaggcat tgtattctat tccacttgtg
     4501 caactgtttg cctgtgtcag ctgtgatttg gcctgtgacc tcagtgcttt ctttgattcc
     4561 acaactctgg ataccattgg caatcttcct gtaaatctaa tcagtgaatg gaaagaattt
     4621 ttttcccaag gcatccacag tttgctttta cctattttgg tgactgttac aggagaaaac
     4681 aaagatgtgt ctgaaacatc ctttcagaat gcaatgctga aacccatgtg tgaaacatta
     4741 acgtatatct caaaggaaca gctattgagt cacaaacttc ctgcaagatt agttgctgac
     4801 caaaaaacaa acttaccaga atatctccag actttgttaa atacattggc cccattactc
     4861 ctcttcagag ctaggcctgt gcaaattgct gtttatcata tgctatacaa attgatgcct
     4921 gaattaccac agtatgatca ggataatcta aagtcatacg gagatgaaga agaagagcca
     4981 gccttgtcac caccagcagc actgatgtct cttcttagca ttcaagagga cttactagaa
     5041 aatgttttgg ggtgtattcc tgttggacag atagttacta ttaaaccact gagtgaagac
     5101 ttctgttatg ttctgggata ccttctcact tggaaattaa tactaacttt cttcaaagct
     5161 gcatcatcac agcttcgggc tttgtattcc atgtatcttc ggaaaacaaa gagtttgaat
     5221 aaattgctct atcacctgtt caggcttatg ccagaaaatc caacctatgc agagacagca
     5281 gttgaggtcc caaataagga ccctaaaaca ttctttactg aggagctcca gctgagtatt
     5341 agagaaacaa caatgcttcc ataccacatt ccacacttgg cttgttcagt ctatcatatg
     5401 acattaaaag acttgcctgc catggttagg ttgtggtgga atagcagtga gaagcgtgtt
     5461 ttcaatattg tggatagatt tacaagcaag tatgtcagca gtgttctttc ttttcaagaa
     5521 atatcttctg tacaaacaag tacacaacta tttaatggca tgacggttaa agctcgagct
     5581 actactcgag aggtaatggc tacttatact attgaggaca tagttattga acttataata
     5641 caactgcctt caaattatcc actgggttca ataatagtag aaagtgggaa aagagtagga
     5701 gtagctgttc agcagtggcg gaactggatg ctgcagttaa gcacttacct cacccatcag
     5761 aatggaagta ttatggaagg cttagcttta tggaaaaata acgtagacaa acgttttgag
     5821 ggtgttgaag attgcatgat ctgtttctca gtcattcacg gtttcaacta ttcccttccc
     5881 aaaaaagcct gtagaacatg caagaaaaaa ttccattcag cctgcttgta caaatggttt
     5941 acatctagca acaaatccac ttgtccactg tgtcgtgaga cgtttttcat cgatgtcgag
     6001 gattacaagg atgacgacga taaggactat aaggacgatg atgacaagga ctacaaagat
     6061 gatgacgata aatagggccc ggtaccttaa ttaattaagg taccaggtaa gtgtacccaa
     6121 ttcgccctat agtgagtcgt attacaattc actcgatcgg ctcgctgatc agcctcgact
     6181 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     6241 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     6301 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     6361 gaagacaata gcaggcatgc tggggaacgc gtaaattgta agcgttaata ttttgttaaa
     6421 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
     6481 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
     6541 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
     6601 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
     6661 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
     6721 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
     6781 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
     6841 gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     6901 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     6961 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     7021 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     7081 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     7141 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     7201 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     7261 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     7321 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     7381 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     7441 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     7501 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     7561 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     7621 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     7681 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     7741 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     7801 gatcgctgag ataggtgcct cactgattaa gcattggcct caggattagt gcttcatccc
     7861 cgtggcccgt tgctcgcgtt tgctggcggt gtccccggaa gaaatatatt tgcatgtctt
     7921 tagttctatg atgacacaaa ccccgcccag cgtcttgtca ttggcgaatt cgaacacgca
     7981 gatgcagtcg gggcggcgcg gtccgaggtc cacttcgcat attaaggtga cgcgtgtggc
     8041 ctcgaacacc gagcgaccct gcagcgaccc gcttaacagc gtcaacagcg tgccgcagat
     8101 cccggggggc aatgagatat gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt
     8161 ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct
     8221 cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc
     8281 gatggtttct acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt
     8341 ccggaagtgc ttgacattgg ggaattcagc gagagcctga cctattgcat ctcccgccgt
     8401 gcacagggtg tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctgcagccg
     8461 gtcgcggagg ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc
     8521 ccattcggac cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt
     8581 gctgatcccc atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc
     8641 gcgcaggctc tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc
     8701 gtgcacgcgg atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc
     8761 attgactgga gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc
     8821 tggaggccgt ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg
     8881 gagcttgcag gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc
     8941 tatcagagct tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac
     9001 gcaatcgtcc gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg
     9061 gccgtctgga ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc
     9121 actcgtccgg atcgggagat gggggaggct aactgaaaca cggaaggaga caataccgga
     9181 aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacgggt gttgggtcgt
     9241 ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac
     9301 cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg
     9361 gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata gccactggcc
     9421 ccgtgggtta gggacggggt cccccatggg gaatggttta tggttcgtgg gggttattat
     9481 tttgggcgtt gcgtggggtc aggtccacga ctggactgag cagacagacc catggttttt
     9541 ggatggcctg ggcatggacc gcatgtactg gcgcgacacg aacaccgggc gtctgtggct
     9601 gccaaacacc cccgaccccc aaaaaccacc gcgcggattt ctggcgccgc cggacgaact
     9661 aaacctgact acgacatctc tgccccttct tcgctggtac gaggagcgct ttgcctcagg
     9721 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     9781 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     9841 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     9901 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     9961 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
    10021 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
    10081 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
    10141 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
    10201 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
    10261 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
    10321 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
    10381 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
    10441 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcc
//
