LOCUS       sequence-310766-       22280 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Episomal expression of dSpCas9-VPR. Filamentous fungi vector with 
            AMA1 and riboB selection marker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 22280)
  AUTHORS   Roux I, Woodcraft C, Hu J, Wolters R, Gilchrist CLM, Chooi YH
  TITLE     CRISPR-mediated activation of biosynthetic gene clusters for 
            bioactive molecule discovery in filamentous fungi.
  JOURNAL   ACS Synth Biol. 2020 Jun 11. doi: 10.1021/acssynbio.0c00197.
  PUBMED    32526136
REFERENCE   2  (bases 1 to 22280)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2020 Jun 11. doi: 10.1021/acssynbio.0c00197."
COMMENT     Sequence Label: pCRI006-pYFAC-riboB-PgpdA-dSpCas9-VPR-TtrpC
FEATURES             Location/Qualifiers
     source          1..22280
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     2802..2823
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4114..4130)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4115..4131)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             join(4160..4186,4187..4189,4190..6673,6674..6679,6680..6745,
                     6746..6748,6749..8263)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=Cas9m4
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the mutations D10A, D839A, 
                     H840A, and N863A (Mali et al., 2013)"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVAAIVPQSFLKDDSIDNKVLTRSDKARGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             8276..8296
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8321..8470
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             8495..8515
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8945..9301
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     CDS             9320..9889
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     CDS             join(14364..14432,14433..15224)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(14582..14601)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      15395..15983
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15884..15903
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     16137..16154
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    16271..16292
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(16307..16312,16313..16330,16331..16337)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    16345..16361
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     16350..16372
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     16369..16385
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     16369..16385
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      16402..17744
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(17365..17412)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     17785..17803
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(17841..17863)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     17963..17982
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        18006..18221
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             18222..19025
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     primer_bind     complement(19307..19324)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(19307..19323)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(19316..19338)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(19623..19642)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      join(19664..22280,1..2632)
                     /label=AMA1
                     /note="replication origin from Aspergillus nidulans 
                     (Aleksenko and Clutterbuck, 1996)"
ORIGIN
        1 tgcagatttt gacctagtta acaaggcaca tcatgctatc acttccaaca ctcttgctga
       61 gcgcgttctg ccttgccttt ctggttctac gaagtatcca gtcccgcctg caacgccgca
      121 gaaacgccaa aaggtggggt tgtcaaccgc cagctagagc tccatcggga ttctttggca
      181 ttccgccgtt cctcggtggt cgcagataac tccctaaatt ccctgagcta actctaagtc
      241 gaccatgccg tttatggtta gcgcctccca aaaaggaatg gccgacttag agttacctct
      301 tgaccgactt tttctttcct cccccttaca tttcgttacc acaacacatt cctatatcca
      361 aactccaggt acataactag tcgaaatctc ttttaaatct agtcaagaac tagatactta
      421 accttcatac tgcttagaga tagcctttga agctcttatt tcgctctttg tctcactctc
      481 accttcctcc tcctcctcca atcctttttg ctcgggacta gtccaagact agtcaacgat
      541 tagtatgccg agcattcgcg ataacgatct ccgaaagtcc ccagagtact gtcactatct
      601 cgaggcagtt aaggacgggg agcttacgct gccggatttc aagatagtaa gccgacccga
      661 ctagctttaa gcctagttac tgactagtcc acaggacgac aatggcgtgc ctgatatcca
      721 tccatatgaa gtctactgcc gagtgaaggg atgcctcaag cgtacagtga gtctactgct
      781 atactagttt ctgactagtt attaactagt tgacaggttc cctctgccaa cagaaacata
      841 ttggtcaagc acttgaagga caagaactcc cacggcatgg agtttacatt gcacaatgga
      901 cctcccacta tgaaggaact gatggaggcc aaaggcaagt cctatctaga ttacttggtg
      961 actagtccct gactagtcaa gtagcatggt atgaaggctt gtttgaaggc actgttctcc
     1021 caaccccgac tcctaccaag aagcgcaagc gagctgcgta agtttctgtg agtctaacta
     1081 gtgtattagc taatatatag cagcaccaag tccaaggacc acaatactaa gggtgtcgag
     1141 aattcgtgag tttcttctcc catttcaact agtccttgac tagtcactaa ctactttgca
     1201 gcaacgaggg tgaagctgga aatgatcagg acaatggcga gggcccgtaa gtacagccat
     1261 tcaatgcaga ctagttgcta actaatctgt gactagttca agtggtccgt acgccgtgca
     1321 tacccctgtg actggtagga atttgagcaa gcctgtcttg ccgcgcgatg agaaaggaaa
     1381 ggcaagttac attcagcccc gtacctagga tcagtgctaa tttataacct ctagccactc
     1441 tttatgcaga tccgccgtga gggtagcaag gcagctaaat cagctggtga gaaaggaacc
     1501 ataccctgca agacctgtcg caacgcaaag ggcaaaggta agctatccaa gctagtttgg
     1561 gactagattc taactagtct cagcaccgtg tggttcaaag ccatattgcg agttttggcg
     1621 ctttttctca tcgattgacg aggcaaaggg agcgagtatg caacctcgta agtcagactc
     1681 agacaaagac caactagtta ttgaccagtc ttctagaagg ctctgttgtg gatcttgagg
     1741 ccctggagag ttcctccaac aatccggaga caagcaagtc ctcgtcggac tagtcactaa
     1801 ctagactcta actagttgca gacatggata atgcaaaaga gacaagcaat gaagaaagtg
     1861 gtaagacatt tctcctttgt ggttctggac tagtctttga ctagtcacag tcttaaacaa
     1921 ggaaaatgag catgaaaatg aggaggaaaa ggctgctgag cccgaggaag tgcagggtga
     1981 tggcagacat ggtaggttaa taccttgtta gttattgcta gtcactgact agtcaataac
     2041 tagtctctga acaccttgca atcactccgt ttgcgcagct gaacagtggt gaggataata
     2101 gtagtaagtt attctagctt cagagttata ggagactaga tactaactag tattagttgc
     2161 aactaacctg gatctcagag actttggcct caatctagaa tctatctagt tgtcaactag
     2221 actgtggtat cattgtcttt tattttccta gtcctggaac tagcttctaa ctagtctccc
     2281 taatatgtgg ctgtcttgtt tttttttttt gtttccctac ccggatatct agtccccttc
     2341 taggttctgt taacctctcg ggctctgatt tagtttaacg caaacctgag attagtttct
     2401 aactagtctc taggttttct atccaccttt aattgtaata ataaatacaa gcaacgttta
     2461 tacgtcaaaa gcatttataa acttttaccc taaagtagct tgcttgtgtg tttagtttat
     2521 aattagtctc ttattaattt gatgtaggta agcccgccac aaatatatat ttttaacaag
     2581 ataccgtgga aaaacttcgt gctatcacaa aacagtatac aaaaaataag ctggctttcc
     2641 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     2701 cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac
     2761 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     2821 tggaacaaca ctcaactcta tctcgggcta ttcttttgat ttataaggga ttttgccgat
     2881 ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     2941 aatggggtga cgatgagccg ctcttgcatc tttgtttgta ttatactgtc tttcttgtta
     3001 cacataatta ttctagaatg ccccaccgtt acatacggga cacagccatt tacatatgca
     3061 tgtggattac gagctaacga gttcattcaa atctcagaac tatcacataa tcatcattcc
     3121 cctatcgtca aagaccgtaa gacaaatccg gttcatgcac tgaacccatt cgggtagtga
     3181 gtcatttact cagcacactc gcgctgacgc tcgtcgaaca ccttcaatgc ctcctcggca
     3241 gccttgacac cactgagaac catggcaccg aaggtagggc ccatgcggtt aaagccatca
     3301 atttcagaca gctccatacc gccgattatc aagcccttag taacctcgcg ggtgttcttg
     3361 acgatggcat cctgcggccg catgcggaga gacggacgga cgcagagaga agggctgagt
     3421 aataagcgcc actgcgccag acagctctgg cggctctgag gtgcagtgga tgattattaa
     3481 tccgggaccg gccgcccctc cgccccgaag tggaaaggct ggtgtgcccc tcgttgacca
     3541 agaatctatt gcatcatcgg agaatatgga gcttcatcga atcaccggca gtaagcgaag
     3601 gagaatgtga agccaggggt gtatagccgt cggcgaaata gcatgccatt aacctaggta
     3661 cagaagtcca attgcttccg atctggtaaa agattcacga gatagtacct tctccgaagt
     3721 aggtagagcg agtacccggc gcgtaagctc cctaattggc ccatccggca tctgtagggc
     3781 gtccaaatat cgtgcctctc ctgctttgcc cggtgtatga aaccggaaag gccgctcagg
     3841 agctggccag cggcgcagac cgggaacaca agctggcagt cgacccatcc ggtgctctgc
     3901 actcgacctg ctgaggtccc tcagtccctg gtaggcagct ttgccccgtc tgtccgcccg
     3961 gtgtgtcggc ggggttgaca aggtcgttgc gtcagtccaa catttgttgc catattttcc
     4021 tgctctcccc accagctgct cttttctttt ctctttcttt tcccatcttc agtatattca
     4081 tcttcccatc caagaacctt taatcaagct tatcgatacc gtcgacctcg actctagagg
     4141 atcactcgtc gtgtcgccca tggacaagaa gtactccatt gggctcgcta tcggcacaaa
     4201 cagcgtcggc tgggccgtca ttacggacga gtacaaggtg ccgagcaaaa aattcaaagt
     4261 tctgggcaat accgatcgcc acagcataaa gaagaacctc attggcgccc tcctgttcga
     4321 ctccggggag acggccgaag ccacgcggct caaaagaaca gcacggcgca gatatacccg
     4381 cagaaagaat cggatctgct acctgcagga gatctttagt aatgagatgg ctaaggtgga
     4441 tgactctttc ttccataggc tggaggagtc ctttttggtg gaggaggata aaaagcacga
     4501 gcgccaccca atctttggca atatcgtgga cgaggtggcg taccatgaaa agtacccaac
     4561 catatatcat ctgaggaaga agcttgtaga cagtactgat aaggctgact tgcggttgat
     4621 ctatctcgcg ctggcgcata tgatcaaatt tcggggacac ttcctcatcg agggggacct
     4681 gaacccagac aacagcgatg tcgataaact ctttatccaa ctggttcaga cttacaatca
     4741 gcttttcgaa gagaacccga tcaacgcatc cggagttgac gccaaagcaa tcctgagcgc
     4801 taggctgtcc aaatcccggc ggctcgaaaa cctcatcgca cagctccctg gggagaagaa
     4861 gaacggcctg tttggtaatc ttatcgccct gtcactcggg ctgaccccca actttaaatc
     4921 taacttcgac ctggccgaag atgccaagct tcaactgagc aaagacacct acgatgatga
     4981 tctcgacaat ctgctggccc agatcggcga ccagtacgca gacctttttt tggcggcaaa
     5041 gaacctgtca gacgccattc tgctgagtga tattctgcga gtgaacacgg agatcaccaa
     5101 agctccgctg agcgctagta tgatcaagcg ctatgatgag caccaccaag acttgacttt
     5161 gctgaaggcc cttgtcagac agcaactgcc tgagaagtac aaggaaattt tcttcgatca
     5221 gtctaaaaat ggctacgccg gatacattga cggcggagca agccaggagg aattttacaa
     5281 atttattaag cccatcttgg aaaaaatgga cggcaccgag gagctgctgg taaagcttaa
     5341 cagagaagat ctgttgcgca aacagcgcac tttcgacaat ggaagcatcc cccaccagat
     5401 tcacctgggc gaactgcacg ctatcctcag gcggcaagag gatttctacc cctttttgaa
     5461 agataacagg gaaaagattg agaaaatcct cacatttcgg ataccctact atgtaggccc
     5521 cctcgcccgg ggaaattcca gattcgcgtg gatgactcgc aaatcagaag agaccatcac
     5581 tccctggaac ttcgaggaag tcgtggataa gggggcctct gcccagtcct tcatcgaaag
     5641 gatgactaac tttgataaaa atctgcctaa cgaaaaggtg cttcctaaac actctctgct
     5701 gtacgagtac ttcacagttt ataacgagct caccaaggtc aaatacgtca cagaagggat
     5761 gagaaagcca gcattcctgt ctggagagca gaagaaagct atcgtggacc tcctcttcaa
     5821 gacgaaccgg aaagttaccg tgaaacagct caaagaagac tatttcaaaa agattgaatg
     5881 tttcgactct gttgaaatca gcggagtgga ggatcgcttc aacgcatccc tgggaacgta
     5941 tcacgatctc ctgaaaatca ttaaagacaa ggacttcctg gacaatgagg agaacgagga
     6001 cattcttgag gacattgtcc tcacccttac gttgtttgaa gatagggaga tgattgaaga
     6061 acgcttgaaa acttacgctc atctcttcga cgacaaagtc atgaaacagc tcaagaggcg
     6121 ccgatataca ggatgggggc ggctgtcaag aaaactgatc aatgggatcc gagacaagca
     6181 gagtggaaag acaatcctgg attttcttaa gtccgatgga tttgccaacc ggaacttcat
     6241 gcagttgatc catgatgact ctctcacctt taaggaggac atccagaaag cacaagtttc
     6301 tggccagggg gacagtcttc acgagcacat cgctaatctt gcaggtagcc cagctatcaa
     6361 aaagggaata ctgcagaccg ttaaggtcgt ggatgaactc gtcaaagtaa tgggaaggca
     6421 taagcccgag aatatcgtta tcgagatggc ccgagagaac caaactaccc agaagggaca
     6481 gaagaacagt agggaaagga tgaagaggat tgaagagggt ataaaagaac tggggtccca
     6541 aatccttaag gaacacccag ttgaaaacac ccagcttcag aatgagaagc tctacctgta
     6601 ctacctgcag aacggcaggg acatgtacgt ggatcaggaa ctggacatca atcggctctc
     6661 cgactacgac gtggctgcta tcgtgcccca gtcttttctc aaagatgatt ctattgataa
     6721 taaagtgttg acaagatccg ataaagctag agggaagagt gataacgtcc cctcagaaga
     6781 agttgtcaag aaaatgaaaa attattggcg gcagctgctg aacgccaaac tgatcacaca
     6841 acggaagttc gataatctga ctaaggctga acgaggtggc ctgtctgagt tggataaagc
     6901 cggcttcatc aaaaggcagc ttgttgagac acgccagatc accaagcacg tggcccaaat
     6961 tctcgattca cgcatgaaca ccaagtacga tgaaaatgac aaactgattc gagaggtgaa
     7021 agttattact ctgaagtcta agctggtctc agatttcaga aaggactttc agttttataa
     7081 ggtgagagag atcaacaatt accaccatgc gcatgatgcc tacctgaatg cagtggtagg
     7141 cactgcactt atcaaaaaat atcccaagct tgaatctgaa tttgtttacg gagactataa
     7201 agtgtacgat gttaggaaaa tgatcgcaaa gtctgagcag gaaataggca aggccaccgc
     7261 taagtacttc ttttacagca atattatgaa ttttttcaag accgagatta cactggccaa
     7321 tggagagatt cggaagcgac cacttatcga aacaaacgga gaaacaggag aaatcgtgtg
     7381 ggacaagggt agggatttcg cgacagtccg gaaggtcctg tccatgccgc aggtgaacat
     7441 cgttaaaaag accgaagtac agaccggagg cttctccaag gaaagtatcc tcccgaaaag
     7501 gaacagcgac aagctgatcg cacgcaaaaa agattgggac cccaagaaat acggcggatt
     7561 cgattctcct acagtcgctt acagtgtact ggttgtggcc aaagtggaga aagggaagtc
     7621 taaaaaactc aaaagcgtca aggaactgct gggcatcaca atcatggagc gatcaagctt
     7681 cgaaaaaaac cccatcgact ttctcgaggc gaaaggatat aaagaggtca aaaaagacct
     7741 catcattaag cttcccaagt actctctctt tgagcttgaa aacggccgga aacgaatgct
     7801 cgctagtgcg ggcgagctgc agaaaggtaa cgagctggca ctgccctcta aatacgttaa
     7861 tttcttgtat ctggccagcc actatgaaaa gctcaaaggg tctcccgaag ataatgagca
     7921 gaagcagctg ttcgtggaac aacacaaaca ctaccttgat gagatcatcg agcaaataag
     7981 cgaattctcc aaaagagtga tcctcgccga cgctaacctc gataaggtgc tttctgctta
     8041 caataagcac agggataagc ccatcaggga gcaggcagaa aacattatcc acttgtttac
     8101 tctgaccaac ttgggcgcgc ctgcagcctt caagtacttc gacaccacca tagacagaaa
     8161 gcggtacacc tctacaaagg aggtcctgga cgccacactg attcatcagt caattacggg
     8221 gctctatgaa acaagaatcg acctctctca gctcggtgga gacagcaggg ctgaccccaa
     8281 gaagaagagg aaggtggagg ccagcggttc cggacgggct gacgcattgg acgattttga
     8341 tctggatatg ctgggaagtg acgccctcga tgattttgac cttgacatgc ttggttcgga
     8401 tgcccttgat gactttgacc tcgacatgct cggcagtgac gcccttgatg atttcgacct
     8461 ggacatgctg attaactcta gaagttccgg atctccgaaa aagaaacgca aagttggtag
     8521 ccagtacctg cccgacaccg acgaccggca ccggatcgag gaaaagcgga agcggaccta
     8581 cgagacattc aagagcatca tgaagaagtc ccccttcagc ggccccaccg accctagacc
     8641 tccacctaga agaatcgccg tgcccagcag atccagcgcc agcgtgccaa aacctgcccc
     8701 ccagccttac cccttcacca gcagcctgag caccatcaac tacgacgagt tccctaccat
     8761 ggtgttcccc agcggccaga tctctcaggc ctctgctctg gctccagccc ctcctcaggt
     8821 gctgcctcag gctcctgctc ctgcaccagc tccagccatg gtgtctgcac tggctcaggc
     8881 accagcaccc gtgcctgtgc tggctcctgg acctccacag gctgtggctc caccagcccc
     8941 taaacctaca caggccggcg agggcacact gtctgaagct ctgctgcagc tgcagttcga
     9001 cgacgaggat ctgggagccc tgctgggaaa cagcaccgat cctgccgtgt tcaccgacct
     9061 ggccagcgtg gacaacagcg agttccagca gctgctgaac cagggcatcc ctgtggcccc
     9121 tcacaccacc gagcccatgc tgatggaata ccccgaggcc atcacccggc tcgtgacagg
     9181 cgctcagagg cctcctgatc cagctcctgc ccctctggga gcaccaggcc tgcctaatgg
     9241 actgctgtct ggcgacgagg acttcagctc tatcgccgac atggacttct ccgcactgct
     9301 gggtagcgga tcgggatctc gggattccag ggaagggatg tttttgccga agcctgaggc
     9361 cggctccgct attagtgacg tgtttgaggg ccgcgaggtg tgccagccaa aacgaatccg
     9421 gccatttcat cctccaggaa gtccatgggc caaccgccca ctccccgcca gcctcgcacc
     9481 aacaccaacc ggtccagtac atgagccagt cgggtcactg accccggcac cagtccctca
     9541 gccactggat ccagcgcccg cagtgactcc cgaggccagt cacctgttgg aggatcccga
     9601 tgaagagacg agccaggctg tcaaagccct tcgggagatg gccgatactg tgattcccca
     9661 gaaggaagag gctgcaatct gtggccaaat ggacctttcc catccgcccc caaggggcca
     9721 tctggatgag ctgacaacca cacttgagtc catgaccgag gatctgaacc tggactcacc
     9781 cctgaccccg gaattgaacg agattctgga taccttcctg aacgacgagt gcctcttgca
     9841 tgccatgcat atcagcacag gactgtccat cttcgacaca tctctgtttt agtgggcgac
     9901 gacgacgttc gagggggggc ccggtaccgc cccgtccggt cctgcccgtc accgagatcc
     9961 acttaacgtt actgaaatca tcaaacagct tgacgaatct ggatataaga tcgttggtgt
    10021 cgatgtcagc tccggagttg agacaaatgg tgttcaggat ctcgataaga tacgttcatt
    10081 tgtccaagca gcaaagagtg ccttctagtg atttaatagc tccatgtcaa caagaataaa
    10141 acgcgtttcg ggtttacctc ttccagatac agctcatctg caatgcatgc ggccgctgga
    10201 tgactccctt tcgatgatac cctttagcag aagtgttctt aaattgcggg aagaggccgt
    10261 tcaggagtct ggctcgtttg atcacatggg attaaaatct ggtgttgagg ctgggatatt
    10321 ttatgttttg tctcttgcgc cccttaacgt gagttttcgt tccactgagc gtcagacccc
    10381 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
    10441 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
    10501 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatacctt ctagtgtagc
    10561 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
    10621 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
    10681 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
    10741 ccagcttgga gcgaacgacc tacaccgaac tgagagawct ncgataagat acgttcattt
    10801 gtccaagcag caaagagtgc cttctagtga tttaatagct ccatgtcaac aagaataaaa
    10861 cgcgtttcgg gtttacctct tccagataca gctcatctgc aatgcatgcg gccgctggat
    10921 gactcccttt cgatgatacc ctttagcaga agtgttctta aattgcggga agaggccgtt
    10981 caggagtctg gctcgtttga tcacatggga ttaaaatctg gtgttgaggc tgggatattt
    11041 tatgttttgt ctcttgcgct tcgatatcaa cagattgaca ataatgaata actatctacg
    11101 gactatgaat gcagctcgat ctactccgta tttagtcggg caatctcccg tagaagatgc
    11161 tagactgcgc tgaaagacta ggcacagtta attgccgcgg atcacgtcag gcctgacaaa
    11221 tgacaggtgg accttcatgt aatcgcacga tgagtcagcg cctcttctcc cccgccgact
    11281 tgcagctgcg gatgtcccga ggagccgaac attgaatcta tcccaagagt gactgaaata
    11341 tgattgcaat cgtcgtcttt ttcttgcaga gaatgtggtg aggtgatcaa tcgtgttttt
    11401 cttcatatat tatattctgt aatgatcata ctttgttgag atacccgtct ctaaaattgt
    11461 actttcgcct ccctcctccc tctcaagtcc gtcatcgcaa tgtcgaattt accgtcccat
    11521 gcttctccgt cttttggggc gcaaacgcct accacgccgc agcccgtcga cgagctttct
    11581 ctcaagtcga acatcacatc agcccctgcg ttacactcca gattagacga actccgagat
    11641 gagacttcca gccagatcag cagcatagac actgaccgcg gggacatgac ccctgcggtt
    11701 cccgcatcat tactctcccc atccttcact cctccagcaa cccccggggg ctcgatcaat
    11761 accgcggagc tgcttcaaca gacccaatta gctggcgcac ggcaagggtc aacacatact
    11821 aagcccccga ggttgctttc tcgtcttccc aatgtcgaat gtatcgtccg cgctcggata
    11881 cccacgacaa ctggcgcgga gatgtttctt cacctgtacc acaatgactt agacaacaaa
    11941 gagcatttgg cgattgtgtt tggaaataca attcgaagca gaagcttgga caagatccgg
    12001 cctggagaga cggagatgga tcgcatgatc cgtggcgcgt atgtcgggaa actccgaccg
    12061 ggccgtgtga gcagctggta tgacgaggag aaagctgaag aggccgccag tccggaagct
    12121 ggcggcgctg tatccagtcc gcacacactg ccgcgaagta gcctcaagga ggcacctctt
    12181 gtgagaatcc attcggagtg ctacactggt gagacggctt ggtctgcacg ctgcgactgc
    12241 ggcgagcagc tcgatgaagc agctcgcctc atgtcgctgc ctatggaaac gttggcagag
    12301 gctgcgtcac cgccggatgg ggcggtaccg tcaaatgctg ctggaggtgt aattgtgtac
    12361 ctgcgccaag aaggacgagg cattgggctg ggcgagaaac tgaaagcgta caatcttcag
    12421 gatctgggat cagacacggt ggaagccaat ctcctgcttc gccaccctgc ggatgcaaga
    12481 agttatggat tagctacggc gatcctggtg gatcttggtc tagggattga ctcgaatccg
    12541 cacggcatcc gactactcac gaataacccg gacaagattc gagcggttga gggacccaac
    12601 cgagaagtgg tggtgaaaga gcgggtgccg atggtgccat tggcatggag atcgggtgga
    12661 aagatgggaa tcaagagttc cgaggtcgaa gggtatctga gaacgaaggt atgtcgacaa
    12721 tctatctctt gcaagctagc ttattttctc atcattcttg aacaatgtct cactctttcc
    12781 tcgcagatat cgaaaatggg ccatttgctc caatgaacct atcccctttc ggttagtaac
    12841 cttgtgctgg aagtacgcct tcctgctttt ggacttagcc aagtcgacac ggtgtatgct
    12901 cgtcacactc atgtaacggt tctgcagcgc aaacgttctg gtcacgttat tcgcttttgt
    12961 ccattttgtc tttttgtgcg gtacataaac tagaggatta gcgcactttg tacatgatgt
    13021 tcccgacatg tcacattagg cctcgattgg tcaaaataat ccctctctaa cctctggttc
    13081 gcatactctt aaatgcagct ctagtctcta gacggactag ttcctcctgc agtggatgtc
    13141 tgacgagtgt ctgcaaggac cgttttccgt gtccagttag ttccaatcac ccggatatcg
    13201 gcgagacgtc ggccgattga gatcactgag tcaatggcgt tgagtggcaa ttaatatatc
    13261 tggatgccta gaatcctata aattgggaaa actagagacc ccggtctatc gtggtggcag
    13321 tgcgtcattt tttcctcttg gagcaaaagt gtagtgccag tacgagtgtt gtggaggaag
    13381 gctgcataca ttgtgcctgt cattaaacga tgagctcgtc cgtattggcc cctgtaatgc
    13441 catgttttcc gcccccaatc gtcaaggttt tccctttgtt agattcctac cagtcatcta
    13501 gcaagtgagg taagctttgc cagaaacgcc aaggctttat ctatgtagtc gataagcaaa
    13561 gtggactgat agcttaatat ggaaggtccc tcaggacaag tcgacctgtg cagaagagat
    13621 aacagcttgg catcacgcat cagtgcctcc tctcagacag aataagtcgc cataagttat
    13681 cgaccgaacc taggtagggt atatttggtg acgacaatac ctcccgacga tacctggatt
    13741 agacaccgta tgccgcgaat taacgccttc cgaccaagtc ccttttggtg atcgacactt
    13801 taggatcatc tcctgccagt cgcccagact gacggtgtta tggtggcgcg ctatggcttt
    13861 cctagccggt caagctccgt gcactagaga gcggagaagg agggggagtt gatagggaat
    13921 gatatctagg tagagaagta ctccaaccag agatatgcgg atcaatgctc cggcagttgt
    13981 tagcataccc caacctcgtt taagtatcct cttcttccat ccatctttat cattatttgt
    14041 ctatgtgcat ccctagatca cgtcaattat ttcgataaat ggatacgtgc actcgggaat
    14101 gcgtgattgg ctcgtctcgt gttctcagtt cctcattccc ccccacgtta cattgacaac
    14161 ggagtattag cctatggtcc tgttcgataa gcttgctcca tcgcctccac tacgtcaagc
    14221 ggagtatcag acatgagctc taccccagat atcgcctgct tatcgctggt tgctttgtca
    14281 tgcaagtaca gatcacatcg gcaattctga taacagatgg tgccattgtc ccgtaatcaa
    14341 ttgcccattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
    14401 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
    14461 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
    14521 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
    14581 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
    14641 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
    14701 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
    14761 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
    14821 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
    14881 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
    14941 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
    15001 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
    15061 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
    15121 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
    15181 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
    15241 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
    15301 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
    15361 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
    15421 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
    15481 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
    15541 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
    15601 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
    15661 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
    15721 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
    15781 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
    15841 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
    15901 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
    15961 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
    16021 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
    16081 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
    16141 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
    16201 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
    16261 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
    16321 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
    16381 atgaccatga ttacgccact gaacgaagca tctgtgcttc attttgtaga acaaaaatgc
    16441 aacgcgagag cgctaatttt tcaaacaaag aatctgagct gcatttttac agaacagaaa
    16501 tgcaacgcga aagcgctatt ttaccaacga agaatctgtg cttcattttt gtaaaacaaa
    16561 aatgcaacgc gagagcgcta atttttcaaa caaagaatct gagctgcatt tttacagaac
    16621 agaaatgcaa cgcgagagcg ctattttacc aacaaagaat ctatacttct tttttgttct
    16681 acaaaaatgc atcccgagag cgctattttt ctaacaaagc atcttagatt actttttttc
    16741 tcctttgtgc gctctataat gcagtctctt gataactttt tgcactgtag gtccgttaag
    16801 gttagaagaa ggctactttg gtgtctattt tctcttccat aaaaaaagcc tgactccact
    16861 tcccgcgttt actgattact agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc
    16921 ccgattatat tctataccga tgtggattgc gcatactttg tgaacagaaa gtgatagcgt
    16981 tgatgattct tcattggtca gaaaattatg aacggtttct tctattttgt ctctatatac
    17041 tacgtatagg aaatgtttac attttcgtat tgttttcgat tcactctatg aatagttctt
    17101 actacaattt ttttgtctaa agagtaatac tagagataaa cataaaaaat gtagaggtcg
    17161 agtttagatg caagttcaag gagcgaaagg tggatgggta ggttatatag ggatatagca
    17221 cagagatata tagcaaagag atacttttga gcaatgtttg tggaagcggt attcgcaata
    17281 ttttagtagc tcgttacagt ccggtgcgtt tttggttttt tgaaagtgcg tcttcagagc
    17341 gcttttggtt ttcaaaagcg ctctgaagtt cctatacttt ctagagaata ggaacttcgg
    17401 aataggaact tcaaagcgtt tccgaaaacg agcgcttccg aaaatgcaac gcgagctgcg
    17461 cacatacagc tcactgttca cgtcgcacct atatctgcgt gttgcctgta tatatatata
    17521 catgagaaga acggcatagt gcgtgtttat gcttaaatgc gtacttatat gcgtctattt
    17581 atgtaggatg aaaggtagtc tagtacctcc tgtgatatta tcccattcca tgcggggtat
    17641 cgtatgcttc cttcagcact accctttagc tgttctatat gctgccactc ctcaattgga
    17701 ttagtctcat ccttcaatgc tatcatttcc tttgatattg gatcatacta agaaaccatt
    17761 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt
    17821 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
    17881 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
    17941 tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatacc
    18001 accttttcaa ttcatcattt tttttttatt cttttttttg atttcggttt ccttgaaatt
    18061 tttttgattc ggtaatctcc gaacagaagg aagaacgaag gaaggagcac agacttagat
    18121 tggtatatat acgcatatgt agtgttgaag aaacatgaaa ttgcccagta ttcttaaccc
    18181 aactgcacag aacaaaaacc tgcaggaaac gaagataaat catgtcgaaa gctacatata
    18241 aggaacgtgc tgctactcat cctagtcctg ttgctgccaa gctatttaat atcatgcacg
    18301 aaaagcaaac aaacttgtgt gcttcattgg atgttcgtac caccaaggaa ttactggagt
    18361 tagttgaagc attaggtccc aaaatttgtt tactaaaaac acatgtggat atcttgactg
    18421 atttttccat ggagggcaca gttaagccgc taaaggcatt atccgccaag tacaattttt
    18481 tactcttcga agacagaaaa tttgctgaca ttggtaatac agtcaaattg cagtactctg
    18541 cgggtgtata cagaatagca gaatgggcag acattacgaa tgcacacggt gtggtgggcc
    18601 caggtattgt tagcggtttg aagcaggcgg cagaagaagt aacaaaggaa cctagaggcc
    18661 ttttgatgtt agcagaattg tcatgcaagg gctccctatc tactggagaa tatactaagg
    18721 gtactgttga cattgcgaag agcgacaaag attttgttat cggctttatt gctcaaagag
    18781 acatgggtgg aagagatgaa ggttacgatt ggttgattat gacacccggt gtgggtttag
    18841 atgacaaggg agacgcattg ggtcaacagt atagaaccgt ggatgatgtg gtctctacag
    18901 gatctgacat tattattgtt ggaagaggac tatttgcaaa gggaagggat gctaaggtag
    18961 agggtgaacg ttacagaaaa gcaggctggg aagcatattt gagaagatgc ggccagcaaa
    19021 actaaaaaac tgtattataa gtaaatgcat gtatactaaa ctcacaaatt agagcttcaa
    19081 tttaattata tcagttatta ccctatgcgg tgtgaaatac cgcacagatg cgtaaggaga
    19141 aaataccgca tcaggaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
    19201 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
    19261 aagaatagac cgagataggg ttgagtgggg taccgagctc gaattcactg gccgtcgttt
    19321 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
    19381 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
    19441 tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg catctgtgcg
    19501 gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag
    19561 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccttagcgcc
    19621 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccagcttat tttttgtata
    19681 ctgttttgtg atagcacgaa gtttttccac ggtatcttgt taaaaatata tatttgtggc
    19741 gggcttacct acatcaaatt aataagagac taattataaa ctaaacacac aagcaagcta
    19801 ctttagggta aaagtttata aatgcttttg acgtataaac gttgcttgta tttattatta
    19861 caattaaagg tggatagaaa acctagagac tagttagaaa ctaatctcag gtttgcgtta
    19921 aactaaatca gagcccgaga ggttaacaga acctagaagg ggactagata tccgggtagg
    19981 gaaacaaaaa aaaaaaacaa gacagccaca tattagggag actagttaga agctagttcc
    20041 aggactagga aaataaaaga caatgatacc acagtctagt tgacaactag atagattcta
    20101 gattgaggcc aaagtctctg agatccaggt tagttgcaac taatactagt tagtatctag
    20161 tctcctataa ctctgaagct agaataactt actactatta tcctcaccac tgttcagctg
    20221 cgcaaacgga gtgattgcaa ggtgttcaga gactagttat tgactagtca gtgactagca
    20281 ataactaaca aggtattaac ctaccatgtc tgccatcacc ctgcacttcc tcgggctcag
    20341 cagccttttc ctcctcattt tcatgctcat tttccttgtt taagactgtg actagtcaaa
    20401 gactagtcca gaaccacaaa ggagaaatgt cttaccactt tcttcattgc ttgtctcttt
    20461 tgcattatcc atgtctgcaa ctagttagag tctagttagt gactagtccg acgaggactt
    20521 gcttgtctcc ggattgttgg aggaactctc cagggcctca agatccacaa cagagccttc
    20581 tagaagactg gtcaataact agttggtctt tgtctgagtc tgacttacga ggttgcatac
    20641 tcgctccctt tgcctcgtca atcgatgaga aaaagcgcca aaactcgcaa tatggctttg
    20701 aaccacacgg tgctgagact agttagaatc tagtcccaaa ctagcttgga tagcttacct
    20761 ttgccctttg cgttgcgaca ggtcttgcag ggtatggttc ctttctcacc agctgattta
    20821 gctgccttgc taccctcacg gcggatctgc ataaagagtg gctagaggtt ataaattagc
    20881 actgatccta ggtacggggc tgaatgtaac ttgcctttcc tttctcatcg cgcggcaaga
    20941 caggcttgct caaattccta ccagtcacag gggtatgcac ggcgtacgga ccacttgaac
    21001 tagtcacaga ttagttagca actagtctgc attgaatggc tgtacttacg ggccctcgcc
    21061 attgtcctga tcatttccag cttcaccctc gttgctgcaa agtagttagt gactagtcaa
    21121 ggactagttg aaatgggaga agaaactcac gaattctcga cacccttagt attgtggtcc
    21181 ttggacttgg tgctgctata tattagctaa tacactagtt agactcacag aaacttacgc
    21241 agctcgcttg cgcttcttgg taggagtcgg ggttgggaga acagtgcctt caaacaagcc
    21301 ttcataccat gctacttgac tagtcaggga ctagtcacca agtaatctag ataggacttg
    21361 cctttggcct ccatcagttc cttcatagtg ggaggtccat tgtgcaatgt aaactccatg
    21421 ccgtgggagt tcttgtcctt caagtgcttg accaatatgt ttctgttggc agagggaacc
    21481 tgtcaactag ttaataacta gtcagaaact agtatagcag tagactcact gtacgcttga
    21541 ggcatccctt cactcggcag tagacttcat atggatggat atcaggcacg ccattgtcgt
    21601 cctgtggact agtcagtaac taggcttaaa gctagtcggg tcggcttact atcttgaaat
    21661 ccggcagcgt aagctccccg tccttaactg cctcgagata gtgacagtac tctggggact
    21721 ttcggagatc gttatcgcga atgctcggca tactaatcgt tgactagtct tggactagtc
    21781 ccgagcaaaa aggattggag gaggaggagg aaggtgagag tgagacaaag agcgaaataa
    21841 gagcttcaaa ggctatctct aagcagtatg aaggttaagt atctagttct tgactagatt
    21901 taaaagagat ttcgactagt tatgtacctg gagtttggat ataggaatgt gttgtggtaa
    21961 cgaaatgtaa gggggaggaa agaaaaagtc ggtcaagagg taactctaag tcggccattc
    22021 ctttttggga ggcgctaacc ataaacggca tggtcgactt agagttagct cagggaattt
    22081 agggagttat ctgcgaccac tggactctca ggctttccat tggggtacta acatagccat
    22141 caaatgccgg cccgtgttta atctcctcga gatggctggg ctcacatgcg ttctactcaa
    22201 aaactcttat acctaggccc tctcccaatc gtgtatgttt ggaccttcct ctctcacact
    22261 ttggataaca tcaagcatac
//
