LOCUS       sequence-310767-        7391 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Hu STT3A -flag mammalian expression vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7391)
  AUTHORS   Li S, Wang L, Berman M, Kong YY, Dorf ME
  TITLE     Mapping a dynamic innate immunity protein interaction network 
            regulating type I interferon production.
  JOURNAL   Immunity. 2011 Sep 23;35(3):426-40. Epub 2011 Sep 8.
  PUBMED    21903422
REFERENCE   2  (bases 1 to 7391)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Immunity.";
            date: "2011-09-23"; volume: "35(3)"; pages: "426-40. Epub 2011 Sep 
            8"
COMMENT     Sequence Label: pCMV3TAG8li-hSTT3A-3FLAG
FEATURES             Location/Qualifiers
     source          1..7391
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        67..370
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        371..574
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     525..545
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        620..638
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             2899..2970
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     primer_bind     complement(3023..3042)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3024..3042)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3071..3088)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3077..3284
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(3291..3746)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3428..3449)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3640..3659
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3773..3877
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3878..3946,3947..4735)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4096..4115)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        4829..4974
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             5017..6054
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(6052..6071)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6096..6144
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6695..7283
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7184..7203
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
       61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      541 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      601 gcgattacgc caagctcgaa attaaccctc actaaaggga acaaaagctg gctcgagctg
      661 aagcttcatg gatccaaagc ggccgctgaa cggatggctg agggagcccc gcggatcgtt
      721 taggaaagcc ggccagctga tcgtcgtgtg ttgccaccca ttcatgtcaa gatgactaag
      781 tttggatttt tgcgattgtc ctatgagaag caggacacac ttttgaagct tctcattctg
      841 tcaatggctg ctgtattatc cttctccact cgtctgtttg ctgtcctgag atttgaaagt
      901 gttatccatg agtttgatcc gtactttaat tatcggacta ccaggttcct ggctgaggag
      961 gggttttata aattccataa ctggtttgat gaccgagcct ggtacccttt gggacgaatc
     1021 attggaggaa caatttaccc aggtttaatg atcacctctg ctgcaatcta ccatgtactc
     1081 cattttttcc acatcaccat cgacattcgg aatgtctgtg tgttcctggc ccctctcttc
     1141 tcctccttca ccaccatcgt cacgtaccac cttaccaaag agctcaagga tgcaggggct
     1201 gggcttcttg ctgctgccat gattgctgta gttcctggat atatctcccg atctgtggct
     1261 ggctcctatg ataatgaagg gattgccatc ttttgcatgc tactcaccta ctacatgtgg
     1321 atcaaggcag taaagactgg ttccatctgt tgggcagcta agtgtgccct tgcttatttc
     1381 tacatggtct cgtcatgggg aggttatgtg ttcctgatca acttaattcc tctccacgtc
     1441 ctcgtgctga tgctcacagg ccgtttctct caccggatct atgtggccta ctgtactgtt
     1501 tactgcctgg gcactatact ttctatgcag atctcctttg tgggtttcca gcctgtcctt
     1561 tcatcagagc acatggcagc ctttggggtc tttggtctct gccagatcca tgcctttgtg
     1621 gattacctgc gcagcaagtt gaatccacaa caatttgaag ttcttttccg gagcgtcatc
     1681 tctctggtag gctttgtcct tctcaccgtg ggagctctcc tcatgctgac aggaaaaata
     1741 tctccctgga cggggcgttt ctactcgctg ctggatccct cttatgctaa gaacaacatc
     1801 cccatcattg cttctgtgtc tgagcatcag cccacaacct ggtcctcata ctattttgac
     1861 ctgcagctcc tcgtcttcat gtttccagtt ggcctctatt actgctttag caacctgtct
     1921 gatgcccgga tttttatcat catgtatggt gtgaccagca tgtacttttc agctgtaatg
     1981 gtgcgtctaa tgctagtgtt ggcacctgtt atgtgcattc tctctggcat tggagtctcc
     2041 caggtgctgt ccacatacat gaagaatctg gacataagtc gtccagacaa gaagagcaag
     2101 aagcaacagg attccaccta ccctattaag aatgaagtgg caagtgggat gatactggtc
     2161 atggctttct ttctcatcac ctacaccttt cattcaacct gggtgaccag tgaggcctac
     2221 tcttctccgt ccattgtact atctgcccgt ggtggggatg gcagtaggat catatttgat
     2281 gacttccgag aagcatatta ttggcttcgt cataatactc cagaggatgc gaaggtcatg
     2341 tcctggtggg attatggcta tcagattaca gctatggcaa accgaacaat tttagtggac
     2401 aataacacat ggaataatac ccatatttct cgagtagggc aggcaatggc gtccacagag
     2461 gaaaaagcct atgagatcat gagggagctc gatgtcagct atgtgctggt catttttgga
     2521 ggcctcactg ggtattcctc tgatgatatc aacaagtttc tttggatggt ccggattgga
     2581 gggagcacag atacaggcaa acatatcaag gagaatgact attatactcc aactggggag
     2641 ttccgtgtgg accgtgaagg ttctccagtg ctgctcaact gcctcatgta caagatgtgt
     2701 tactatcgct ttggacaggt ttacacagaa gccaagcgtc ctccaggctt tgaccgtgtc
     2761 cgaaatgctg agattgggaa taaagacttt gagcttgatg tcctggagga agcatatacc
     2821 acagaacatt ggctggtcag gatatacaag gtaaaggacc tggataatcg aggcttgtca
     2881 aggacaatcg atgtcgagga ttacaaggat gacgacgata aggactataa ggacgatgat
     2941 gacaaggact acaaagatga tgacgataaa tagggcccgg taccttaatt aattaaggta
     3001 ccaggtaagt gtacccaatt cgccctatag tgagtcgtat tacaattcac tcgatcggct
     3061 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     3121 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     3181 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     3241 agcaaggggg aggattggga agacaatagc aggcatgctg gggaacgcgt aaattgtaag
     3301 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
     3361 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
     3421 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
     3481 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
     3541 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
     3601 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
     3661 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
     3721 gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac
     3781 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     3841 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     3901 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     3961 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     4021 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     4081 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     4141 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     4201 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     4261 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     4321 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     4381 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     4441 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     4501 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     4561 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     4621 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     4681 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggcctca
     4741 ggattagtgc ttcatccccg tggcccgttg ctcgcgtttg ctggcggtgt ccccggaaga
     4801 aatatatttg catgtcttta gttctatgat gacacaaacc ccgcccagcg tcttgtcatt
     4861 ggcgaattcg aacacgcaga tgcagtcggg gcggcgcggt ccgaggtcca cttcgcatat
     4921 taaggtgacg cgtgtggcct cgaacaccga gcgaccctgc agcgacccgc ttaacagcgt
     4981 caacagcgtg ccgcagatcc cggggggcaa tgagatatga aaaagcctga actcaccgcg
     5041 acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg tctccgacct gatgcagctc
     5101 tcggagggcg aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg
     5161 cgggtaaata gctgcgccga tggtttctac aaagatcgtt atgtttatcg gcactttgca
     5221 tcggccgcgc tcccgattcc ggaagtgctt gacattgggg aattcagcga gagcctgacc
     5281 tattgcatct cccgccgtgc acagggtgtc acgttgcaag acctgcctga aaccgaactg
     5341 cccgctgttc tgcagccggt cgcggaggcc atggatgcga tcgctgcggc cgatcttagc
     5401 cagacgagcg ggttcggccc attcggaccg caaggaatcg gtcaatacac tacatggcgt
     5461 gatttcatat gcgcgattgc tgatccccat gtgtatcact ggcaaactgt gatggacgac
     5521 accgtcagtg cgtccgtcgc gcaggctctc gatgagctga tgctttgggc cgaggactgc
     5581 cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca acaatgtcct gacggacaat
     5641 ggccgcataa cagcggtcat tgactggagc gaggcgatgt tcggggattc ccaatacgag
     5701 gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta tggagcagca gacgcgctac
     5761 ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc tccgggcgta tatgctccgc
     5821 attggtcttg accaactcta tcagagcttg gttgacggca atttcgatga tgcagcttgg
     5881 gcgcagggtc gatgcgacgc aatcgtccga tccggagccg ggactgtcgg gcgtacacaa
     5941 atcgcccgca gaagcgcggc cgtctggacc gatggctgtg tagaagtact cgccgatagt
     6001 ggaaaccgac gccccagcac tcgtccggat cgggagatgg gggaggctaa ctgaaacacg
     6061 gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac
     6121 gcacgggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg
     6181 tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac
     6241 cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc
     6301 ctgccatagc cactggcccc gtgggttagg gacggggtcc cccatgggga atggtttatg
     6361 gttcgtgggg gttattattt tgggcgttgc gtggggtcag gtccacgact ggactgagca
     6421 gacagaccca tggtttttgg atggcctggg catggaccgc atgtactggc gcgacacgaa
     6481 caccgggcgt ctgtggctgc caaacacccc cgacccccaa aaaccaccgc gcggatttct
     6541 ggcgccgccg gacgaactaa acctgactac gacatctctg ccccttcttc gctggtacga
     6601 ggagcgcttt gcctcaggtc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     6661 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     6721 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     6781 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     6841 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     6901 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     6961 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     7021 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     7081 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     7141 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     7201 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     7261 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     7321 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     7381 gtattaccgc c
//
