LOCUS       sequence-310771-       15769 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fungal vector to express an LbCas12a crRNA array targeted to Pelca, 
            to be contransformed with pCRI009 containing PelcA-mCherry in a 
            dLbCas12a-VPR strain as proof-of-concept of CRISPRa..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15769)
  AUTHORS   Roux I, Woodcraft C, Hu J, Wolters R, Gilchrist CLM, Chooi YH
  TITLE     CRISPR-mediated activation of biosynthetic gene clusters for 
            bioactive molecule discovery in filamentous fungi.
  JOURNAL   ACS Synth Biol. 2020 Jun 11. doi: 10.1021/acssynbio.0c00197.
  PUBMED    32526136
REFERENCE   2  (bases 1 to 15769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2020 Jun 11. doi: 10.1021/acssynbio.0c00197."
COMMENT     Sequence Label: pCRI011-pYFAC-pyroA-PgpdA-4xcrRNAarrayelcA-TtrpC
FEATURES             Location/Qualifiers
     source          1..15769
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     2802..2823
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4109..4125)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4110..4126)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             join(7853..7921,7922..8713)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8071..8090)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8884..9472
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9373..9392
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9626..9643
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9760..9781
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9796..9801,9802..9819,9820..9826)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9834..9850
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9839..9861
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9858..9874
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9858..9874
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      9891..11233
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(10854..10901)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     11274..11292
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(11330..11352)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     11452..11471
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        11495..11710
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             11711..12514
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     primer_bind     complement(12796..12813)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(12796..12812)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12805..12827)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(13112..13131)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      join(13153..15769,1..2632)
                     /label=AMA1
                     /note="replication origin from Aspergillus nidulans 
                     (Aleksenko and Clutterbuck, 1996)"
ORIGIN
        1 tgcagatttt gacctagtta acaaggcaca tcatgctatc acttccaaca ctcttgctga
       61 gcgcgttctg ccttgccttt ctggttctac gaagtatcca gtcccgcctg caacgccgca
      121 gaaacgccaa aaggtggggt tgtcaaccgc cagctagagc tccatcggga ttctttggca
      181 ttccgccgtt cctcggtggt cgcagataac tccctaaatt ccctgagcta actctaagtc
      241 gaccatgccg tttatggtta gcgcctccca aaaaggaatg gccgacttag agttacctct
      301 tgaccgactt tttctttcct cccccttaca tttcgttacc acaacacatt cctatatcca
      361 aactccaggt acataactag tcgaaatctc ttttaaatct agtcaagaac tagatactta
      421 accttcatac tgcttagaga tagcctttga agctcttatt tcgctctttg tctcactctc
      481 accttcctcc tcctcctcca atcctttttg ctcgggacta gtccaagact agtcaacgat
      541 tagtatgccg agcattcgcg ataacgatct ccgaaagtcc ccagagtact gtcactatct
      601 cgaggcagtt aaggacgggg agcttacgct gccggatttc aagatagtaa gccgacccga
      661 ctagctttaa gcctagttac tgactagtcc acaggacgac aatggcgtgc ctgatatcca
      721 tccatatgaa gtctactgcc gagtgaaggg atgcctcaag cgtacagtga gtctactgct
      781 atactagttt ctgactagtt attaactagt tgacaggttc cctctgccaa cagaaacata
      841 ttggtcaagc acttgaagga caagaactcc cacggcatgg agtttacatt gcacaatgga
      901 cctcccacta tgaaggaact gatggaggcc aaaggcaagt cctatctaga ttacttggtg
      961 actagtccct gactagtcaa gtagcatggt atgaaggctt gtttgaaggc actgttctcc
     1021 caaccccgac tcctaccaag aagcgcaagc gagctgcgta agtttctgtg agtctaacta
     1081 gtgtattagc taatatatag cagcaccaag tccaaggacc acaatactaa gggtgtcgag
     1141 aattcgtgag tttcttctcc catttcaact agtccttgac tagtcactaa ctactttgca
     1201 gcaacgaggg tgaagctgga aatgatcagg acaatggcga gggcccgtaa gtacagccat
     1261 tcaatgcaga ctagttgcta actaatctgt gactagttca agtggtccgt acgccgtgca
     1321 tacccctgtg actggtagga atttgagcaa gcctgtcttg ccgcgcgatg agaaaggaaa
     1381 ggcaagttac attcagcccc gtacctagga tcagtgctaa tttataacct ctagccactc
     1441 tttatgcaga tccgccgtga gggtagcaag gcagctaaat cagctggtga gaaaggaacc
     1501 ataccctgca agacctgtcg caacgcaaag ggcaaaggta agctatccaa gctagtttgg
     1561 gactagattc taactagtct cagcaccgtg tggttcaaag ccatattgcg agttttggcg
     1621 ctttttctca tcgattgacg aggcaaaggg agcgagtatg caacctcgta agtcagactc
     1681 agacaaagac caactagtta ttgaccagtc ttctagaagg ctctgttgtg gatcttgagg
     1741 ccctggagag ttcctccaac aatccggaga caagcaagtc ctcgtcggac tagtcactaa
     1801 ctagactcta actagttgca gacatggata atgcaaaaga gacaagcaat gaagaaagtg
     1861 gtaagacatt tctcctttgt ggttctggac tagtctttga ctagtcacag tcttaaacaa
     1921 ggaaaatgag catgaaaatg aggaggaaaa ggctgctgag cccgaggaag tgcagggtga
     1981 tggcagacat ggtaggttaa taccttgtta gttattgcta gtcactgact agtcaataac
     2041 tagtctctga acaccttgca atcactccgt ttgcgcagct gaacagtggt gaggataata
     2101 gtagtaagtt attctagctt cagagttata ggagactaga tactaactag tattagttgc
     2161 aactaacctg gatctcagag actttggcct caatctagaa tctatctagt tgtcaactag
     2221 actgtggtat cattgtcttt tattttccta gtcctggaac tagcttctaa ctagtctccc
     2281 taatatgtgg ctgtcttgtt tttttttttt gtttccctac ccggatatct agtccccttc
     2341 taggttctgt taacctctcg ggctctgatt tagtttaacg caaacctgag attagtttct
     2401 aactagtctc taggttttct atccaccttt aattgtaata ataaatacaa gcaacgttta
     2461 tacgtcaaaa gcatttataa acttttaccc taaagtagct tgcttgtgtg tttagtttat
     2521 aattagtctc ttattaattt gatgtaggta agcccgccac aaatatatat ttttaacaag
     2581 ataccgtgga aaaacttcgt gctatcacaa aacagtatac aaaaaataag ctggctttcc
     2641 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     2701 cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac
     2761 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     2821 tggaacaaca ctcaactcta tctcgggcta ttcttttgat ttataaggga ttttgccgat
     2881 ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     2941 aatggggtga cgatgagccg ctcttgcatc tttgtttgta ttatactgtc tttcttgtta
     3001 cacataatta ttctagaatg ccccaccgtt acatacggga cacagccatt tacatatgca
     3061 tgtggattac gagctaacga gttcattcaa atctcagaac tatcacataa tcatcattcc
     3121 cctatcgtca aagaccgtaa gacaaatccg gttcatgcac tgaacccatt cgggtagtga
     3181 gtcatttact cagcacactc gcgctgacgc tcgtcgaaca ccttcaatgc ctcctcggca
     3241 gccttgacac cactgagaac catggcaccg aaggtagggc ccatgcggtt aaagccatca
     3301 atttcagaca gctccatacc gccgattatc aagcccttag taacctcgcg ggtgttcttg
     3361 acgatggcat cctgccatgc ggagagacgg acggtcgcag agagaagggc tgagtaataa
     3421 gcgccactgc gccagacagc tctggcggct ctgaggtgca gtggatgatt attaatccgg
     3481 gaccggccgc ccctccgccc cgaagtggaa aggctggtgt gcccctcgtt gaccaagaat
     3541 ctattgcatc atcggagaat atggagcttc atcgaatcac cggcagtaag cgaaggagaa
     3601 tgtgaagcca ggggtgtata gccgtcggcg aaatagcatg ccattaacct aggtacagaa
     3661 gtccaattgc ttccgatctg gtaaaagatt cacgagatag taccttctcc gaagtaggta
     3721 gagcgagtac ccggcgcgta agctccctaa ttggcccatc cggcatctgt agggcgtcca
     3781 aatatcgtgc ctctcctgct ttgcccggtg tatgaaaccg gaaaggccgc tcaggagctg
     3841 gccagcggcg cagaccggga acacaagctg gcagtcgacc catccggtgc tctgcactcg
     3901 acctgctgag gtccctcagt ccctggtagg cagctttgcc ccgtctgtcc gcccggtgtg
     3961 tcggcggggt tgacaaggtc gttgcgtcag tccaacattt gttgccatat tttcctgctc
     4021 tccccaccag ctgctctttt cttttctctt tcttttccca tcttcagtat attcatcttc
     4081 ccatccaaga acctttaatc aagcttatcg ataccgtcga cctcgactct agaggatcga
     4141 atttctacta agtgtagata ttccactcat catagtacta atttctacta agtgtagatg
     4201 atggtggtgc tagcttctca atttctacta agtgtagatc tcaacactgt actggcaaca
     4261 atttctacta agtgtagata acatccttgt gtgaatatta atttctacta agtgtagata
     4321 tcttcgaggg ggggcccggt accgccccgt ccggtcctgc ccgtcaccga gatccactta
     4381 acgttactga aatcatcaaa cagcttgacg aatctggata taagatcgtt ggtgtcgatg
     4441 tcagctccgg agttgagaca aatggtgttc aggatctcga taagatacgt tcatttgtcc
     4501 aagcagcaaa gagtgccttc tagtgattta atagctccat gtcaacaaga ataaaacgcg
     4561 tttcgggttt acctcttcca gatacagctc atctgcaatg catcccctgg tatcatggtt
     4621 gttgggtctc atcactccta ctcaacattg cacctgatag ggacatcaga tgctggatta
     4681 ctaaggtaat gtaaggtcag ttcgagacca tctgatatta ccacaaatac aatggcgaga
     4741 gagtttttcg taaaagccaa tccttggcgt ttccagctgt tcctgacggt tgtaggccca
     4801 agtccgcggg aaaccgccca caaagcggcg tttttgcaga ttggcagatt tatgctggaa
     4861 acttactggg gagatggagg ggcacaagcg ctgtgattgg ttttcaaagc cgcggccgga
     4921 tggaacgaag acataattcg gcggggacat gaaaatgtgg gtgatcgata cggaattttt
     4981 ggttcttcgg aggcgacaaa gggcgcaacg gtcgaggtta gtagttatct tgactcacac
     5041 ttacagggcc cgtcttcggt cttcttaaga actgggtttt gctgggactt cccccccacc
     5101 tctcttttct actgtgtctc gtatctattt ctatactcat tctttcactt ctcttagtac
     5161 caccattccc ttctaaatac acagaatggc ttccaacggt accaatggcg cctccgcctc
     5221 caacagcttc actgtgaagg ccggcttggc tcagatgctg aagggtggtg tgattatgga
     5281 cgtcgtcaac gcggagcagg tatgagcgat tgtcatcagg atacttccag ccctttgacg
     5341 ctaacatgac ttctacaaca ggcccgcatt gcggaggagg ccggtgccgc tgccgtgatg
     5401 gccctggaga gagtccccgc cgacatcaga gcccagggtg gcgttgcccg catgtctgac
     5461 cccagcatga tcaaggagat catggctgct gttaccattc ctgtcatggc caaagctcgt
     5521 atcggacact tcgttgagtg ccaggtaagg ctgcctttct cccgtggaaa gcctgcattg
     5581 cagctaacat gtgtaattgt tagatcctcg aagccattgg cgttgactac atcgacgagt
     5641 ccgaagtcct tacccctgcc gatgatgtct accacgtgaa gaagcacgac tacaaggttc
     5701 ctttcgtctg tggttgccgc aacctgggcg aggcccttcg tcggatcgcc gagggtgccg
     5761 ctatgatccg taccaagggt gaggccggta ccggagatgt tgttgaagcc gtcaagcaca
     5821 tgcgcacggt caactcccag atcgcccgcg cccgctccat cctccagaat tccaccgacc
     5881 ccgagattga gctgcgtgcc tacgctcgtg agcttgaggt cccttatgag cttctgcgcg
     5941 agaccgccga gaagggccgt cttcccgttg tcaacttcgc cgccggcggt gttgccactc
     6001 ccgctgatgc cgcactcatg atgcagctgg gctgcgacgg agtgttcgtc ggctctggta
     6061 ttttcaagtc tggtgatgcg aagaagcgcg ccaaggctat tgtccaggcc gtgactcact
     6121 acaaggaccc caaggtcctc gctgaagtca gcgagggtct gggtgaggcc atggttggta
     6181 tcaatgtctc tcagatgccc gaggccgacc gattggccaa gagaggatgg taattgcact
     6241 actatctcta cttgtgattc ttcttatgtt cttgtcatga tatgggcgtt ggaaaagttg
     6301 atatagcgtt ctttgatgca ttttgcattc aagactttca ggttcattct tgttagggtg
     6361 ttctgtgcat ttgtccttca ttatgtagac actcgcagga ttgaagaaga actactctat
     6421 tcaatgacga tctcttccat gaagaccaaa aactaaaaat tggtcatgtc aacgtccagt
     6481 gcacacgaca ttttaccctt ccccactaac acttgttttg gctgtcgatc atgtggatgc
     6541 tgctactggt ggtgctaatc tgcttgaaga cgcggcgagt agtatcacca agtaatatac
     6601 ttatattgcg aagataattt atatctgata tttcaattac aaattctgag accactaccc
     6661 agttgacggc actcatgctg ggtgccttag atagacgtca aggtacacat cctacggagt
     6721 tacgatcggc gacttcaaaa gaatgtcaca ccggtaagat acacacgttt atttcaaacg
     6781 ccgaacttga atacacaccc tgacagcgcc ataagacaat ttcctcttgg agcaaaagtg
     6841 tagtgccagt acgagtgttg tggaggaagg ctgcatacat tgtgcctgtc attaaacgat
     6901 gagctcgtcc gtattggccc ctgtaatgcc atgttttccg cccccaatcg tcaaggtttt
     6961 ccctttgtta gattcctacc agtcatctag caagtgaggt aagctttgcc agaaacgcca
     7021 aggctttatc tatgtagtcg ataagcaaag tggactgata gcttaatatg gaaggtccct
     7081 caggacaagt cgacctgtgc agaagagata acagcttggc atcacgcatc agtgcctcct
     7141 ctcagacaga ataagtcgcc ataagttatc gaccgaacct aggtagggta tatttggtga
     7201 cgacaatacc tcccgacgat acctggatta gacaccgtat gccgcgaatt aacgccttcc
     7261 gaccaagtcc cttttggtga tcgacacttt aggatcatct cctgccagtc gcccagactg
     7321 acggtgttat ggtggcgcgc tatggctttc ctagccggtc aagctccgtg cactagagag
     7381 cggagaagga gggggagttg atagggaatg atatctaggt agagaagtac tccaaccaga
     7441 gatatgcgga tcaatgctcc ggcagttgtt agcatacccc aacctcgttt aagtatcctc
     7501 ttcttccatc catctttatc attatttgtc tatgtgcatc cctagatcac gtcaattatt
     7561 tcgataaatg gatacgtgca ctcgggaatg cgtgattggc tcgtctcgtg ttctcagttc
     7621 ctcattcccc cccacgttac attgacaacg gagtattagc ctatggtcct gttcgataag
     7681 cttgctccat cgcctccact acgtcaagcg gagtatcaga catgagctct accccagata
     7741 tcgcctgctt atcgctggtt gctttgtcat gcaagtacag atcacatcgg caattctgat
     7801 aacagatggt gccattgtcc cgtaatcaat tgcccattga aaaaggaaga gtatgagtat
     7861 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     7921 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     7981 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     8041 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     8101 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     8161 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     8221 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     8281 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     8341 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     8401 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     8461 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     8521 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     8581 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     8641 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     8701 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     8761 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     8821 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     8881 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     8941 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     9001 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     9061 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     9121 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     9181 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     9241 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     9301 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     9361 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     9421 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     9481 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     9541 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     9601 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     9661 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     9721 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     9781 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     9841 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccactg aacgaagcat
     9901 ctgtgcttca ttttgtagaa caaaaatgca acgcgagagc gctaattttt caaacaaaga
     9961 atctgagctg catttttaca gaacagaaat gcaacgcgaa agcgctattt taccaacgaa
    10021 gaatctgtgc ttcatttttg taaaacaaaa atgcaacgcg agagcgctaa tttttcaaac
    10081 aaagaatctg agctgcattt ttacagaaca gaaatgcaac gcgagagcgc tattttacca
    10141 acaaagaatc tatacttctt ttttgttcta caaaaatgca tcccgagagc gctatttttc
    10201 taacaaagca tcttagatta ctttttttct cctttgtgcg ctctataatg cagtctcttg
    10261 ataacttttt gcactgtagg tccgttaagg ttagaagaag gctactttgg tgtctatttt
    10321 ctcttccata aaaaaagcct gactccactt cccgcgttta ctgattacta gcgaagctgc
    10381 gggtgcattt tttcaagata aaggcatccc cgattatatt ctataccgat gtggattgcg
    10441 catactttgt gaacagaaag tgatagcgtt gatgattctt cattggtcag aaaattatga
    10501 acggtttctt ctattttgtc tctatatact acgtatagga aatgtttaca ttttcgtatt
    10561 gttttcgatt cactctatga atagttctta ctacaatttt tttgtctaaa gagtaatact
    10621 agagataaac ataaaaaatg tagaggtcga gtttagatgc aagttcaagg agcgaaaggt
    10681 ggatgggtag gttatatagg gatatagcac agagatatat agcaaagaga tacttttgag
    10741 caatgtttgt ggaagcggta ttcgcaatat tttagtagct cgttacagtc cggtgcgttt
    10801 ttggtttttt gaaagtgcgt cttcagagcg cttttggttt tcaaaagcgc tctgaagttc
    10861 ctatactttc tagagaatag gaacttcgga ataggaactt caaagcgttt ccgaaaacga
    10921 gcgcttccga aaatgcaacg cgagctgcgc acatacagct cactgttcac gtcgcaccta
    10981 tatctgcgtg ttgcctgtat atatatatac atgagaagaa cggcatagtg cgtgtttatg
    11041 cttaaatgcg tacttatatg cgtctattta tgtaggatga aaggtagtct agtacctcct
    11101 gtgatattat cccattccat gcggggtatc gtatgcttcc ttcagcacta ccctttagct
    11161 gttctatatg ctgccactcc tcaattggat tagtctcatc cttcaatgct atcatttcct
    11221 ttgatattgg atcatactaa gaaaccatta ttatcatgac attaacctat aaaaataggc
    11281 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
    11341 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
    11401 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
    11461 agcagattgt actgagagtg caccatacca ccttttcaat tcatcatttt ttttttattc
    11521 ttttttttga tttcggtttc cttgaaattt ttttgattcg gtaatctccg aacagaagga
    11581 agaacgaagg aaggagcaca gacttagatt ggtatatata cgcatatgta gtgttgaaga
    11641 aacatgaaat tgcccagtat tcttaaccca actgcacaga acaaaaacct gcaggaaacg
    11701 aagataaatc atgtcgaaag ctacatataa ggaacgtgct gctactcatc ctagtcctgt
    11761 tgctgccaag ctatttaata tcatgcacga aaagcaaaca aacttgtgtg cttcattgga
    11821 tgttcgtacc accaaggaat tactggagtt agttgaagca ttaggtccca aaatttgttt
    11881 actaaaaaca catgtggata tcttgactga tttttccatg gagggcacag ttaagccgct
    11941 aaaggcatta tccgccaagt acaatttttt actcttcgaa gacagaaaat ttgctgacat
    12001 tggtaataca gtcaaattgc agtactctgc gggtgtatac agaatagcag aatgggcaga
    12061 cattacgaat gcacacggtg tggtgggccc aggtattgtt agcggtttga agcaggcggc
    12121 agaagaagta acaaaggaac ctagaggcct tttgatgtta gcagaattgt catgcaaggg
    12181 ctccctatct actggagaat atactaaggg tactgttgac attgcgaaga gcgacaaaga
    12241 ttttgttatc ggctttattg ctcaaagaga catgggtgga agagatgaag gttacgattg
    12301 gttgattatg acacccggtg tgggtttaga tgacaaggga gacgcattgg gtcaacagta
    12361 tagaaccgtg gatgatgtgg tctctacagg atctgacatt attattgttg gaagaggact
    12421 atttgcaaag ggaagggatg ctaaggtaga gggtgaacgt tacagaaaag caggctggga
    12481 agcatatttg agaagatgcg gccagcaaaa ctaaaaaact gtattataag taaatgcatg
    12541 tatactaaac tcacaaatta gagcttcaat ttaattatat cagttattac cctatgcggt
    12601 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggaaattg taagcgttaa
    12661 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
    12721 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtggggt
    12781 accgagctcg aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
    12841 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
    12901 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat
    12961 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca
    13021 tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
    13081 accgctacac ttgccagcgc cttagcgccc gctcctttcg ctttcttccc ttcctttctc
    13141 gccacgttcg ccagcttatt ttttgtatac tgttttgtga tagcacgaag tttttccacg
    13201 gtatcttgtt aaaaatatat atttgtggcg ggcttaccta catcaaatta ataagagact
    13261 aattataaac taaacacaca agcaagctac tttagggtaa aagtttataa atgcttttga
    13321 cgtataaacg ttgcttgtat ttattattac aattaaaggt ggatagaaaa cctagagact
    13381 agttagaaac taatctcagg tttgcgttaa actaaatcag agcccgagag gttaacagaa
    13441 cctagaaggg gactagatat ccgggtaggg aaacaaaaaa aaaaaacaag acagccacat
    13501 attagggaga ctagttagaa gctagttcca ggactaggaa aataaaagac aatgatacca
    13561 cagtctagtt gacaactaga tagattctag attgaggcca aagtctctga gatccaggtt
    13621 agttgcaact aatactagtt agtatctagt ctcctataac tctgaagcta gaataactta
    13681 ctactattat cctcaccact gttcagctgc gcaaacggag tgattgcaag gtgttcagag
    13741 actagttatt gactagtcag tgactagcaa taactaacaa ggtattaacc taccatgtct
    13801 gccatcaccc tgcacttcct cgggctcagc agccttttcc tcctcatttt catgctcatt
    13861 ttccttgttt aagactgtga ctagtcaaag actagtccag aaccacaaag gagaaatgtc
    13921 ttaccacttt cttcattgct tgtctctttt gcattatcca tgtctgcaac tagttagagt
    13981 ctagttagtg actagtccga cgaggacttg cttgtctccg gattgttgga ggaactctcc
    14041 agggcctcaa gatccacaac agagccttct agaagactgg tcaataacta gttggtcttt
    14101 gtctgagtct gacttacgag gttgcatact cgctcccttt gcctcgtcaa tcgatgagaa
    14161 aaagcgccaa aactcgcaat atggctttga accacacggt gctgagacta gttagaatct
    14221 agtcccaaac tagcttggat agcttacctt tgccctttgc gttgcgacag gtcttgcagg
    14281 gtatggttcc tttctcacca gctgatttag ctgccttgct accctcacgg cggatctgca
    14341 taaagagtgg ctagaggtta taaattagca ctgatcctag gtacggggct gaatgtaact
    14401 tgcctttcct ttctcatcgc gcggcaagac aggcttgctc aaattcctac cagtcacagg
    14461 ggtatgcacg gcgtacggac cacttgaact agtcacagat tagttagcaa ctagtctgca
    14521 ttgaatggct gtacttacgg gccctcgcca ttgtcctgat catttccagc ttcaccctcg
    14581 ttgctgcaaa gtagttagtg actagtcaag gactagttga aatgggagaa gaaactcacg
    14641 aattctcgac acccttagta ttgtggtcct tggacttggt gctgctatat attagctaat
    14701 acactagtta gactcacaga aacttacgca gctcgcttgc gcttcttggt aggagtcggg
    14761 gttgggagaa cagtgccttc aaacaagcct tcataccatg ctacttgact agtcagggac
    14821 tagtcaccaa gtaatctaga taggacttgc ctttggcctc catcagttcc ttcatagtgg
    14881 gaggtccatt gtgcaatgta aactccatgc cgtgggagtt cttgtccttc aagtgcttga
    14941 ccaatatgtt tctgttggca gagggaacct gtcaactagt taataactag tcagaaacta
    15001 gtatagcagt agactcactg tacgcttgag gcatcccttc actcggcagt agacttcata
    15061 tggatggata tcaggcacgc cattgtcgtc ctgtggacta gtcagtaact aggcttaaag
    15121 ctagtcgggt cggcttacta tcttgaaatc cggcagcgta agctccccgt ccttaactgc
    15181 ctcgagatag tgacagtact ctggggactt tcggagatcg ttatcgcgaa tgctcggcat
    15241 actaatcgtt gactagtctt ggactagtcc cgagcaaaaa ggattggagg aggaggagga
    15301 aggtgagagt gagacaaaga gcgaaataag agcttcaaag gctatctcta agcagtatga
    15361 aggttaagta tctagttctt gactagattt aaaagagatt tcgactagtt atgtacctgg
    15421 agtttggata taggaatgtg ttgtggtaac gaaatgtaag ggggaggaaa gaaaaagtcg
    15481 gtcaagaggt aactctaagt cggccattcc tttttgggag gcgctaacca taaacggcat
    15541 ggtcgactta gagttagctc agggaattta gggagttatc tgcgaccact ggactctcag
    15601 gctttccatt ggggtactaa catagccatc aaatgccggc ccgtgtttaa tctcctcgag
    15661 atggctgggc tcacatgcgt tctactcaaa aactcttata cctaggccct ctcccaatcg
    15721 tgtatgtttg gaccttcctc tctcacactt tggataacat caagcatac
//
