LOCUS       sequence-310776-        8737 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Hu CCDC47 -flag mammalian expression Gateway vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8737)
  AUTHORS   Li S, Wang L, Berman M, Kong YY, Dorf ME
  TITLE     Mapping a dynamic innate immunity protein interaction network 
            regulating type I interferon production.
  JOURNAL   Immunity. 2011 Sep 23;35(3):426-40. Epub 2011 Sep 8.
  PUBMED    21903422
REFERENCE   2  (bases 1 to 8737)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Immunity.";
            date: "2011-09-23"; volume: "35(3)"; pages: "426-40. Epub 2011 Sep 
            8"
COMMENT     Sequence Label: pEXPqcxip-hCCDC47-FLAG
FEATURES             Location/Qualifiers
     source          1..8737
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        309..512
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             513..688
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    751..950
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1151..1567
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1497..1519
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1535..1551
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        1604..1907
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1908..2111
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2108..2132
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2299..2323
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3782..3806)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3808..3831
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    join(3864..4438,4439..4441,4442..4450)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4031..4048)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4258..4277
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4470..5069
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4470..4489)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4966..4986
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5443..5868
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(5971..5993)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6129..6149)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6134..6463
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6314..6449
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6485..6504
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6585..6602)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6756..7344)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6836..6855)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7515..8306,8307..8375))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8138..8157
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8376..8480)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8548..8566
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        join(8666..8737,1..308)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc
      541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc
      601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
      661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc
      721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc
      781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta
      841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc
      901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc
      961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca
     1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct
     1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc
     1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga
     1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt
     1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg
     1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga
     1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa
     1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct
     1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct
     1561 aggcgccgga attgaagatc tgggggatcg atcctctaga gtccgttaca taacttacgg
     1621 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     1681 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     1741 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     1801 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     1861 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     1921 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     1981 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     2041 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     2101 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
     2161 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa
     2221 cggacctgca ggaattgatc cgcggccgca ccggtaggcc tcgtacgctt aattaacgga
     2281 tcggagcggc cgctcttcac aagtttgtac aaaaaagcag gctccaccat gaaagccttc
     2341 cacactttct gtgttgtcct tctggtgttt gggagtgtct ctgaagccaa gtttgatgat
     2401 tttgaggatg aggaggacat agtagagtat gatgataatg acttcgctga atttgaggat
     2461 gtcatggaag actctgttac tgaatctcct caacgggtca taatcactga agatgatgaa
     2521 gatgagacca ctgtggagtt ggaagggcag gatgaaaacc aagaaggaga ttttgaagat
     2581 gcagataccc aggagggaga tactgagagt gaaccatatg atgatgaaga atttgaaggt
     2641 tatgaagaca aaccagatac ttcttctagc aaaaataaag acccaataac gattgttgat
     2701 gttcctgcac acctccagaa cagctgggag agttattatc tagaaatttt gatggtgact
     2761 ggtctgcttg cttatatcat gaattacatc attgggaaga ataaaaacag tcgccttgca
     2821 caggcctggt ttaacactca tagggagctt ttggagagca actttacttt agtgggggat
     2881 gatggaacta acaaagaagc cacaagcaca ggaaagttga accaggagaa tgagcacatc
     2941 tataacctgt ggtgttctgg tcgagtgtgc tgtgagggca tgcttatcca gctgaggttc
     3001 ctcaagagac aagacttact gaatgtcctg gcccggatga tgaggccagt gagtgatcaa
     3061 gtgcaaataa aagtaaccat gaatgatgaa gacatggata cctacgtatt tgctgttggc
     3121 acacggaaag ccttggtgcg actacagaaa gagatgcagg atttgagtga gttttgtagt
     3181 gataaaccta agtctggagc aaagtatgga ctgccggact ctttggccat cctgtcagag
     3241 atgggagaag tcacagacgg aatgatggat acaaagatgg ttcactttct tacacactat
     3301 gctgacaaga ttgaatctgt tcatttttca gaccagttct ctggtccaaa aattatgcaa
     3361 gaggaaggtc agcctttaaa gctacctgac actaagagga cactgttgtt tacatttaat
     3421 gtgcctggct caggtaacac ttacccaaag gatatggagg cactgctacc cctgatgaac
     3481 atggtgattt attctattga taaagccaaa aagttccgac tcaacagaga aggcaaacaa
     3541 aaagcagata agaaccgtgc ccgagtagaa gagaacttct tgaaactgac acatgtgcaa
     3601 agacaggaag cagcacagtc tcggcgggag gagaaaaaaa gagcagagaa ggagcgaatc
     3661 atgaatgagg aagatcctga gaaacagcgc aggctggagg aggctgcatt gaggcgtgag
     3721 caaaagaagt tggaaaagaa gcaaatgaaa atgaaacaaa tcaaagtgaa agccatgttg
     3781 gacccagctt tcttgtacaa agtggtggat tacaaggatg acgacgataa gtgattcgaa
     3841 ggatcctccg atccggaatt ccgcccctct ccctcccccc cccctaacgt tactggccga
     3901 agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg
     3961 tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg
     4021 ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt
     4081 cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac
     4141 cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca
     4201 aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg
     4261 ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg
     4321 ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa
     4381 cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataagct
     4441 tgccacaacc cacaaggaga cgaccttcca tgaccgagta caagcccacg gtgcgcctcg
     4501 ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc cgccgcgttc gccgactacc
     4561 ccgccacgcg ccacaccgtc gacccggacc gccacatcga gcgggtcacc gagctgcaag
     4621 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg
     4681 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga
     4741 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag
     4801 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct
     4861 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg
     4921 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct
     4981 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct
     5041 ggtgcatgac ccgcaagccc ggtgcctgac gcccgcccca cgacccgcag cgcccgaccg
     5101 aaaggagcgc acgaccccat ggctccgacc gaagccaccc ggggcggccc cgccgacccc
     5161 gcacccgccc ccgaggccca ccgactctag tcgagggctg cagcgctgca gaggccgagt
     5221 gcagaactgc tccaaaggga cctcaaggct ttccgaggga cactaggctg actccatcga
     5281 gccagtgtag agataagctt atcgattagt ccaatttgtt aaagacagga tatcagtggt
     5341 ccaggctcta gttttgactc aacaatatca ccagctgaag cctatagagt acgagccata
     5401 gataaaataa aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg
     5461 taggtttggc aagctagctt aagtaacgcc attttgcaag gcatggaaaa atacataact
     5521 gagaatagag aagttcagat caaggtcagg aacagatgga acagggtcga ccctagagaa
     5581 ccatcagatg tttccagggt gccccaagga cctgaaatga ccctgtgcct tatttgaact
     5641 aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct gctccccgag ctcaataaaa
     5701 gagcccacaa cccctcactc ggggcgccag tcctccgatt gactgagtcg cccgggtacc
     5761 cgtgtatcca ataaaccctc ttgcagttgc atccgacttg tggtctcgct gttccttggg
     5821 agggtctcct ctgagtgatt gactacccgt cagcgggggt ctttcatttg ggggctcgtc
     5881 cgggatcggg agacccctgc ccagggacca ccgacccacc accgggaggt aagctggctg
     5941 cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
     6001 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     6061 tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtagat
     6121 ccggctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     6181 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     6241 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     6301 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     6361 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     6421 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctta ctggcttaac
     6481 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
     6541 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg
     6601 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     6661 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     6721 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     6781 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     6841 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     6901 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     6961 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     7021 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     7081 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     7141 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     7201 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     7261 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     7321 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     7381 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     7441 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     7501 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     7561 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     7621 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     7681 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     7741 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     7801 aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt
     7861 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     7921 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     7981 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     8041 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     8101 cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga
     8161 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     8221 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     8281 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     8341 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     8401 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     8461 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     8521 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa
     8581 gaattagctt ggccattgca tacgttgtat ccatatcata atatgtacat ttatattggc
     8641 tcatgtccaa cattaccgcc atgttgacat tgattattga ctagttatta atagtaatca
     8701 attacggggt cattagttca tagcccatat atggagt
//
