LOCUS       sequence-310777-        8092 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Hu SURF4 -flag mammalian expression Gateway vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8092)
  AUTHORS   Li S, Wang L, Berman M, Kong YY, Dorf ME
  TITLE     Mapping a dynamic innate immunity protein interaction network 
            regulating type I interferon production.
  JOURNAL   Immunity. 2011 Sep 23;35(3):426-40. Epub 2011 Sep 8.
  PUBMED    21903422
REFERENCE   2  (bases 1 to 8092)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Immunity.";
            date: "2011-09-23"; volume: "35(3)"; pages: "426-40. Epub 2011 Sep 
            8"
COMMENT     Sequence Label: pEXPqcxip-hSURF4-FLAG
FEATURES             Location/Qualifiers
     source          1..8092
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        309..512
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             513..688
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    751..950
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1151..1567
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1497..1519
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1535..1551
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        1604..1907
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1908..2111
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2108..2132
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2299..2323
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3137..3161)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3163..3186
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    join(3219..3793,3794..3796,3797..3805)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3386..3403)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3613..3632
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3825..4424
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3825..3844)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4321..4341
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4798..5223
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(5326..5348)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5484..5504)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5489..5818
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5669..5804
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5840..5859
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5940..5957)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6111..6699)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6191..6210)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6870..7661,7662..7730))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7493..7512
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7731..7835)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7903..7921
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        join(8021..8092,1..308)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc
      541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc
      601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
      661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc
      721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc
      781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta
      841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc
      901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc
      961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca
     1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct
     1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc
     1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga
     1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt
     1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg
     1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga
     1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa
     1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct
     1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct
     1561 aggcgccgga attgaagatc tgggggatcg atcctctaga gtccgttaca taacttacgg
     1621 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     1681 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     1741 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     1801 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     1861 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     1921 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     1981 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     2041 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     2101 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
     2161 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa
     2221 cggacctgca ggaattgatc cgcggccgca ccggtaggcc tcgtacgctt aattaacgga
     2281 tcggagcggc cgctcttcac aagtttgtac aaaaaagctg gcaccatggg ccagaacgac
     2341 ctgatgggca cggccgagga cttcgccgac cagttcctcc gtgtcacaaa gcagtacctg
     2401 ccccacgtgg cgcgcctctg tctgatcagc accttcctgg aggacggcat ccgtatgtgg
     2461 ttccagtgga gcgagcagcg cgactacatc gacaccacct ggaactgcgg ctacctgctg
     2521 gcctcgtcct tcgtcttcct caacttgctg ggacagctga ctggctgcgt cctggtgttg
     2581 agcaggaact tcgtgcagta cgcctgcttc gggctctttg gaatcatagc tctgcagacg
     2641 attgcctaca gcattttatg ggacttgaag tttttgatga ggaacctggc cctgggagga
     2701 ggcctgttgc tgctcctagc agaatcccgt tctgaaggga agagcatgtt tgcgggcgtc
     2761 cccaccatgc gtgagagctc ccccaaacag tacatgcagc tcggaggcag ggtcttgctg
     2821 gttctgatgt tcatgaccct ccttcacttt gacgccagct tcttttctat tgtccagaac
     2881 atcgtgggca cagctctgat gattttagtg gccattggtt ttaaaaccaa gctggctgct
     2941 ttgactcttg ttgtgtggct ctttgccatc aacgtatatt tcaacgcctt ctggaccatt
     3001 ccagtctaca agcccatgca tgacttcctg aaatacgact tcttccagac catgtcggtg
     3061 attgggggct tgctcctggt ggtggccctg ggccctgggg gtgtctccat ggatgagaag
     3121 aagaaggagt ggggcgaccc agctttcttg tacaaagtgg tggattacaa ggatgacgac
     3181 gataagtgat tcgaaggatc ctccgatccg gaattccgcc cctctccctc ccccccccct
     3241 aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt
     3301 tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg
     3361 acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc
     3421 gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt
     3481 tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta
     3541 taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg
     3601 gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag
     3661 gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag
     3721 tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa
     3781 acacgatgat aagcttgcca caacccacaa ggagacgacc ttccatgacc gagtacaagc
     3841 ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc cgtacgcacc ctcgccgccg
     3901 cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc ggaccgccac atcgagcggg
     3961 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
     4021 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
     4081 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
     4141 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
     4201 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
     4261 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
     4321 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
     4381 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgacgcccg ccccacgacc
     4441 cgcagcgccc gaccgaaagg agcgcacgac cccatggctc cgaccgaagc cacccggggc
     4501 ggccccgccg accccgcacc cgcccccgag gcccaccgac tctagtcgag ggctgcagcg
     4561 ctgcagaggc cgagtgcaga actgctccaa agggacctca aggctttccg agggacacta
     4621 ggctgactcc atcgagccag tgtagagata agcttatcga ttagtccaat ttgttaaaga
     4681 caggatatca gtggtccagg ctctagtttt gactcaacaa tatcaccagc tgaagcctat
     4741 agagtacgag ccatagataa aataaaagat tttatttagt ctccagaaaa aggggggaat
     4801 gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt gcaaggcatg
     4861 gaaaaataca taactgagaa tagagaagtt cagatcaagg tcaggaacag atggaacagg
     4921 gtcgacccta gagaaccatc agatgtttcc agggtgcccc aaggacctga aatgaccctg
     4981 tgccttattt gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc
     5041 ccgagctcaa taaaagagcc cacaacccct cactcggggc gccagtcctc cgattgactg
     5101 agtcgcccgg gtacccgtgt atccaataaa ccctcttgca gttgcatccg acttgtggtc
     5161 tcgctgttcc ttgggagggt ctcctctgag tgattgacta cccgtcagcg ggggtctttc
     5221 atttgggggc tcgtccggga tcgggagacc cctgcccagg gaccaccgac ccaccaccgg
     5281 gaggtaagct ggctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
     5341 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
     5401 gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga
     5461 tagcggagtg tagatccggc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     5521 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     5581 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     5641 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     5701 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     5761 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     5821 gcttactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg
     5881 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc
     5941 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     6001 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     6061 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     6121 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     6181 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     6241 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     6301 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     6361 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     6421 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     6481 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     6541 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     6601 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     6661 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     6721 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     6781 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     6841 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     6901 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     6961 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     7021 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     7081 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     7141 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg
     7201 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     7261 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     7321 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     7381 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     7441 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc
     7501 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     7561 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     7621 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     7681 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     7741 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     7801 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     7861 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     7921 ccctttcgtc ttcaagaatt agcttggcca ttgcatacgt tgtatccata tcataatatg
     7981 tacatttata ttggctcatg tccaacatta ccgccatgtt gacattgatt attgactagt
     8041 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gt
//
