LOCUS       sequence-310815-       10440 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Template plasmid for inserting GCaMP reporter into the Orco gene of 
            Aedes aegypti with CRISPR/Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10440)
  AUTHORS   Zhao Z, Zung JL, Hinze A, Kriete AL, Iqbal A, Younger MA, Matthews 
            BJ, Merhof D, Thiberge S, Ignell R, Strauch M, McBride CS
  TITLE     Mosquito brains encode unique features of human odour to drive host 
            seeking.
  JOURNAL   Nature. 2022 May;605(7911):706-712. doi: 10.1038/s41586-022-04675-4.
            Epub 2022 May 4.
  PUBMED    35508661
REFERENCE   2  (bases 1 to 10440)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-022-04675-4"; journalName: "Nature"; date: 
            "2022-05"; volume: "605"; issue: "7911"; pages: "706-712"
COMMENT     Sequence Label: Orco-T2A-QF2-9xQUAS-GCaMP6f-3XP3-dsRed
FEATURES             Location/Qualifiers
     source          1..10440
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(12..29)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(183..771)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(263..282)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(942..1733,1734..1802))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1565..1584
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1803..1907)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1975..1993
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2031..2053)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2153..2172
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(2189..2644)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2326..2347)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2538..2557
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2842..2864
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2856..2873
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2857..2873
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             4202..4255
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     promoter        5830..6068
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     6015..6036
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             join(6144..6152,6153..6170,6171..6173,6174..6206,6207..6209,
                     6210..6233,6234..6260,6261..6317,6318..6323,6324..6593,
                     6594..6611,6612..7046,7047..7052,7053..7496)
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6f
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
                     TGHAVRAIGRLSSLENVYIKADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGP
                     VLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE
                     LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLT
                     YGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKEEFSLFDKDGDGTITTKELGTVMRS
                     LGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKYRDTEEEIREAFGVFDKDGN
                     GYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     regulatory      6240..6249
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     6530..6551
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(6657..6678)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6918..6937)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     polyA_signal    7618..7739
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      8114..8123
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(8120..8122,8123..8125,8126..8800)
                     /codon_start=1
                     /product="wild-type DsRed"
                     /label=DsRed1
                     /note="mammalian codon-optimized"
                     /translation="MVRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV
                     KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     KALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRH
                     HLFL"
     primer_bind     complement(8303..8323)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     8521..8540
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     8715..8738
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    8921..9042
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10221..10237)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10221..10237)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10234..10256)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10245..10261
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10269..10275,10276..10293,10294..10299))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10314..10335
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
       61 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
      121 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
      181 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
      241 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
      301 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
      361 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
      421 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
      481 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
      541 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
      601 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
      661 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
      721 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
      781 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
      841 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
      901 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
      961 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     1021 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     1081 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     1141 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     1201 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     1261 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     1321 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     1381 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     1441 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     1501 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     1561 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     1621 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     1681 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     1741 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     1801 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     1861 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     1921 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     1981 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     2041 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     2101 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca
     2161 gagcagattg tactgagagt gcaccataaa attgtaaacg ttaatatttt gttaaaattc
     2221 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
     2281 ccttataaat caaaagaata gcccgagata gggttgagtg ttgttccagt ttggaacaag
     2341 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
     2401 gatggcccac tacgtgaacc atcacccaaa tcaagttttt tggggtcgag gtgccgtaaa
     2461 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
     2521 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
     2581 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
     2641 gcgtactatg gttgctttga cgtatgcggt gtgaaatacc gcacagatgc gtaaggagaa
     2701 aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
     2761 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
     2821 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc
     2881 ttaaggtgca cggcccacgt ggccactagt acttctcgag ctctgtacat gtccgcggtc
     2941 gcgacgtacg cgtatcgatg gcgccagctg caggcggccg ccatagagtt tcgcttttcc
     3001 acgcgcggtg tgtctggaat cgttattaac gaaaaaaaat attctaattc gggatttaca
     3061 attctctgtg aatatgaaaa catttcaacg agagaaacga aagttatagt gatttgaagg
     3121 ttttcggcta ttctgtaaga tattctttca aataataggt gccaaattgg tggacatgtt
     3181 ttgtaattaa cgatttcaga cacaccgtgc cacgtttggt tctctctagc aatttgattg
     3241 tgcatttttt ttctctaaca gagttcatca gtgatggagg ctgcctactc gtgccactgg
     3301 tatgacggtt ccgaggaagc caaaactttc gtgcaaatcg tttgccagca gtgtcagaaa
     3361 gcgatgacca tatccggggc caagtttttc accgtttcac tggatctgtt cgcatcggtg
     3421 agtgattttt ttttcgtttt cgattcggtt tctgggggga aattagtgtc ggatcgatcg
     3481 ataaaagcga ttgcatgggc ataaccagaa aggagacatg agtcctcctg cctccacctc
     3541 ccttttgaaa aaaaagctgg gtgaattaat tttgtgtttg tattttcaaa atcgtctata
     3601 tctacaaaat aattgcatca cgattgatat ttctgagaaa tttttgaagt tactcaaaca
     3661 ataattttcc taaaaaagat cttatcagaa attatgagaa ggttttatca acagtccgca
     3721 tttttgaatc aatataataa gtttttctga aaaaaaaaaa tacagaaata cggtgacgaa
     3781 tttctgtgga atttttttga gattcaaata tttcaaaaac atattttatg gtcgtctgca
     3841 aaaaaccgtg agacgttttt gatttcgtgg aatctatctt gaagatttct tcgatgaact
     3901 gtatgaaatt actatgtcga atcaattgaa tctgtagaaa aaatgtctca tccggatttt
     3961 tccaagcctt gtttctaatc gtccactgga ctccactttg gaaaagtgtg aattttttaa
     4021 gtcacttgcc cctccggcta ttctggctac gcccatgacc gattgtataa gaaagccacg
     4081 aaactgagtc aactataatt aatgattcgt cgttttcttt ccgttttcct ttcccccaca
     4141 ggttctggga gcggtcgtca cctacttcat ggttctcgtt caactcaagg atggatcggg
     4201 agagggccgc ggctccctgc tgacctgcgg cgacgtggag gagaaccccg gccccatgcc
     4261 acccaagcgc aaaacgctta acgctgcggc tgaggctaac gctcatgccg acggacacgc
     4321 cgacggaaac gccgacggac acgtggccaa tacggccgcg tcctcgaata atgcgaggtt
     4381 cgctgatctc actaacatcg atactccggg tctgggaccc acaactacga ccctgctcgt
     4441 ggaaccagca cgctcaaagc gtcaacgagt gtcccgcgca tgcgaccagt gccgtgcagc
     4501 ccgagagaaa tgcgacggaa tacagcctgc gtgtttcccg tgcgtttccc agggaaggtc
     4561 ctgcacttat caggcttcgc cgaaaaagag gggagttcaa accggttata ttcgtacgct
     4621 ggagctcgcc ctcgcctgga tgtttgaaaa tgtcgcgcgt tccgaagatg ccttgcataa
     4681 cctcctcgtc cgtgacgccg gacaaggatc agctctgctc gttggtaaag attcgccggc
     4741 tgccgagcga ctccatgccc gttgggctac tagccgtgtc aataagagca ttacccgcct
     4801 cctccgtcag ttggagctcc ctcctaccgc cacggctacg gcctcgataa tgccgcacgt
     4861 gatggagcag cctctcagta ccagcattaa ccccgtcaac gaccgcttca acggtattcc
     4921 caaccccact ccgtataact ccgatgcagc tctcgatgct atcactcaga ccaacgatta
     4981 tggaagcgta aatacacatg gtatcctctc tacttacccg ccaccggcta cgcaccttaa
     5041 tgaagcttcc gtcgctctcg ctcccggtgg cgcccccccc cgaccgcctc ctccgtatgt
     5101 tgacagcacg accaatcacc cgccgtacca ctcgaatctg gttccaatgg cgaactttgg
     5161 ttactcgacc gttgattacg atgccatggt tgacgatttg gctagcattg aatacacgga
     5221 cgctgtggat gtcgacccac agtttatgac caatctggga ttcgttcctg gatgtaactt
     5281 ctccgacatt aatacatacg aacagtgatg acgtctatcg ataccgtcga ctaaagccaa
     5341 atagaaaatt attcagttcc tggcttaagt ttttaaaagt gatattattt atttggttgt
     5401 aaccaaccaa aagaatgtaa ataactaata cataattatg ttagttttaa gttagcaaca
     5461 aattgatttt agctatatta gctacttggt taataaatag aatatattta tttaaagata
     5521 attgcgtttt tattgtcagg gagtgagttt gcttaaaaac tcgtttagat ccgcggccca
     5581 gttggtcctg tagccgtgca gagtggcata tgggtaatcg cttatcctcg gataaacaat
     5641 tatcctcacg ggtaatcgct tatccgctcg ggtaatcgct tatccttggg taatcgctta
     5701 tcctcggata aacaattatc ctcacgggta atcgcttatc cgctcgggta atcgcttatc
     5761 ctcgggtaat cgcttatcct taagcttgat atcgaattcc tgcagcccga gcggagactc
     5821 tagcgagcgc cggagtataa atagaggcgc ttcgtctacg gagcgacaat tcaattcaaa
     5881 caagcaaagt gaacacgtcg ctaagcgaaa gctaagcaaa taaacaagcg cagctgaaca
     5941 agctaaacaa tctgcagtaa agtgcaagtt aaagtgaatc aattaaaagt aaccagcaac
     6001 caagtaaatc aactgcaact actgaaatct gccaagaagt aattattgaa tacaagaaga
     6061 gaactctgaa tagggaattg ggaattcgtt aacagatcgg gggatcaatt cgttaacaga
     6121 tctgcggccg cggctcgagc aaaatgggtt ctcatcatca tcatcatcat ggtatggcta
     6181 gcatgactgg tggacagcaa atgggtcggg atctgtacga cgatgacgat aaggatctcg
     6241 ccaccatggt cgactcatca cgtcgtaagt ggaataagac aggtcacgca gtcagagcta
     6301 taggtcggct gagctcactc gagaacgtct atatcaaggc cgacaagcag aagaacggca
     6361 tcaaggcgaa cttcaagatc cgccacaaca tcgaggacgg cggcgtgcag ctcgcctacc
     6421 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     6481 tgagcgtgca gtccaaactt tcgaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     6541 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagggcggta
     6601 ccggagggag catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     6661 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggtgagggcg
     6721 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     6781 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
     6841 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac atccaggagc
     6901 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     6961 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     7021 tcctggggca caagctggag tacaacctgc cggaccaact gactgaagag cagatcgcag
     7081 aatttaaaga ggaattctcc ctatttgaca aggacgggga tgggacaata acaaccaagg
     7141 agctggggac ggtgatgcgg tctctggggc agaaccccac agaagcagag ctgcaggaca
     7201 tgatcaatga agtagatgcc gacggtgacg gcacaatcga cttccctgag ttcctgacaa
     7261 tgatggcaag aaaaatgaaa tacagggaca cggaagaaga aattagagaa gcgttcggtg
     7321 tgtttgataa ggatggcaat ggctacatca gtgcagcaga gcttcgccac gtgatgacaa
     7381 accttggaga gaagttaaca gatgaagagg ttgatgaaat gatcagggaa gcagacatcg
     7441 atggggatgg tcaggtaaac tacgaagagt ttgtacaaat gatgacagcg aagtgagcga
     7501 tcgcgactct agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
     7561 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
     7621 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     7681 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttac
     7741 ggcgcatctg gctgccaggt ttggccatgc ggaggcctac gcccccaact gagagaactc
     7801 aaaggttacc ccagttgggg cactactcta gatacgcgta cggcaattcg agctcgcccg
     7861 gggatctaat tcaattagag actaattcaa ttagagctaa ttcaattagg atccaagctt
     7921 atcgatttcg aaccctcgac cgccggagta taaatagagg cgcttcgtct acggagcgac
     7981 aattcaattc aaacaagcaa agtgaacacg tcgctaagcg aaagctaagc aaataaacaa
     8041 gcgcagctga acaagctaaa caatcggggt accgctagag tcgacggtac cgcgggcccg
     8101 ggatccaccg gtcgccacca tggtgcgctc ctccaagaac gtcatcaagg agttcatgcg
     8161 cttcaaggtg cgcatggagg gcaccgtgaa cggccacgag ttcgagatcg agggcgaggg
     8221 cgagggccgc ccctacgagg gccacaacac cgtgaagctg aaggtgacca agggcggccc
     8281 cctgcccttc gcctgggaca tcctgtcccc ccagttccag tacggctcca aggtgtacgt
     8341 gaagcacccc gccgacatcc ccgactacaa gaagctgtcc ttccccgagg gcttcaagtg
     8401 ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct
     8461 gcaggacggc tgcttcatct acaaggtgaa gttcatcggc gtgaacttcc cctccgacgg
     8521 ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc accgagcgcc tgtacccccg
     8581 cgacggcgtg ctgaagggcg agatccacaa ggccctgaag ctgaaggacg gcggccacta
     8641 cctggtggag ttcaagtcca tctacatggc caagaagccc gtgcagctgc ccggctacta
     8701 ctacgtggac tccaagctgg acatcacctc ccacaacgag gactacacca tcgtggagca
     8761 gtacgagcgc accgagggcc gccaccacct gttcctgtag cggccgcgac tctagatcat
     8821 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
     8881 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
     8941 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     9001 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcctagtg ttggtgcagt
     9061 tgaaataatt cgttaattat ggacaagctg aaaactatac ggtactattt ttgatactaa
     9121 tgcaactgaa cggcgataca tgacatgaca tcgtttttat atgtagattt ataaggaaga
     9181 aaaaaattat cagtacaaga acaattagac tagaccctcc gacactgagt agcggaggct
     9241 aatgatgttg atatattaaa tattttagta agaatttttg atgtagctga aatagacgct
     9301 caagatgaat gcaatgaact ctcgagaact tattcataac agtgagagaa tgccctgatt
     9361 gttcactgta attagttttc cacacgattt acttggttta tgaaaagccc ttgtagattg
     9421 agaagtcatt agtcaagata tgtcactccc gtgcaaaagt ttgggttcac cccctcaaac
     9481 atatacaaaa gtgttctgtc catatctctg tgattacacg tcaaatttaa actctttaag
     9541 ccgcattcga aaggcaaaga gttattctta cttcgtatgt atttttccaa aaacattttt
     9601 ttaaattttg tatactaaat ttttattaaa gttgtgacat ttttcaaaaa acacccttaa
     9661 aaatcatatc taatttcctc agcattgggt tgaccaaaat ttcaaatcaa agtgtcatta
     9721 gaatcgtaat cttatattcc ttgaagagac accacgaaat ttcggcggac aaatctggaa
     9781 agtattcaaa atcaatgaaa cagtcagtca agtcatcgtg caaaagtttg ggttcatctc
     9841 tcagtatgat gcaaatcgtg caaaagtttg ggttcacctg atccgcacgc acataatttt
     9901 tgtcaatatc tcggccattt ttcaaccgat tttaatagtt taaagctttt ttgagcgcaa
     9961 ataatggcgt gtacatgatt ggtctgagat ttcacagatt taagtaagtt ctaggcctat
    10021 taatattccg gagtatacgt agccggctaa cgttaacaac cggtacctct agaactatag
    10081 ctagcatgcg caaatttaaa gcgctgatat cgatcgcgcg cagatctgtc atgatgatca
    10141 ttgcaattgg atccatatat agggcccggg ttataattac ctcaggtcga cgtcccatgg
    10201 ccattcgaat tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
    10261 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
    10321 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
    10381 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
//
