LOCUS       sequence-310824-        8271 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  making transgenics expressing a UAS driven eGFP and NTR 2.0.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8271)
  AUTHORS   Sharrock AV, Mulligan TS, Hall KR, Williams EM, White DT, Zhang L, 
            Emmerich K, Matthews F, Nimmagadda S, Washington S, Le KD, Meir-Levi
            D, Cox OL, Saxena MT, Calof AL, Lopez-Burks ME, Lander AD, Ding D, 
            Ji H, Ackerley DF, Mumm JS
  TITLE     NTR 2.0: a rationally engineered prodrug-converting enzyme with 
            substantially enhanced efficacy for targeted cell ablation.
  JOURNAL   Nat Methods. 2022 Feb;19(2):205-215. doi: 
            10.1038/s41592-021-01364-4. Epub 2022 Feb 7.
  PUBMED    35132245
REFERENCE   2  (bases 1 to 8271)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-021-01364-4"; journalName: "Nat Methods"; date: 
            "2022-02"; volume: "19"; issue: "2"; pages: "205-215"
COMMENT     Sequence Label: 5xUAS:GAP-EGFP-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2
FEATURES             Location/Qualifiers
     source          1..8271
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    618..739
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(629..648)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     683..702
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     protein_bind    complement(1068..1101)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      1581..1590
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2669..2686)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2675..2900
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    join(3020..3182,3183..3194)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     promoter        complement(3245..3263)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3246..3263)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3281..3297)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3281..3297)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3294..3316)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3305..3321
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3329..3335,3336..3353,3354..3359))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3374..3395
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3512..3529)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3683..4271)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3763..3782)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4442..5233,5234..5302))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5065..5084
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5303..5407)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5485..5940)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5622..5643)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5834..5853
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6066..6088
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6080..6097
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6081..6097
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6104..6123
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6104..6122
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(6135..6146,6147..6334)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     protein_bind    complement(6412..6445)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(6760..6762,6763..6765,6766..7476)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6760..6762,6763..6765,6766..7476)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7066..7085)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7413..7434
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             7498..7554
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     intron          join(8240..8271,1..541)
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
ORIGIN
        1 tttcgctatt gtaaaattca tgttatatgg agggggcaaa gttttcaggg tgttgtttag
       61 aatgggaaga tgtcccttgt atcaccatgg accctcatga taattttgtt tctttcactt
      121 tctactctgt tgacaaccat tgtctcctct tattttcttt tcattttctg taactttttc
      181 gttaaacttt agcttgcatt tgtaacgaat ttttaaattc acttttgttt atttgtcaga
      241 ttgtaagtac tttctctaat cacttttttt tcaaggcaat cagggtatat tatattgtac
      301 ttcagcacag ttttagagaa caattgttat aattaaatga taaggtagaa tatttctgca
      361 tataaattct ggctggcgtg gaaatattct tattggtaga aacaactaca ccctggtcat
      421 catcctgcct ttctctttat ggttacaatg atatacactg tttgagatga ggataaaata
      481 ctctgagtcc aaaccgggcc cctctgctaa ccatgttcat gccttcttct ctttcctaca
      541 gctcctgggc aacgtgctgg ttgttgtgct gtctcatcat tttggcaaag aattaattcc
      601 tcgacgtcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
      661 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
      721 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
      781 ggaggtgtgg gaggtttttt gtcgcgaatc tagattaaga ttgaatcctg ttgccggtct
      841 tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta
      901 atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta
      961 atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc
     1021 atctatgtta ctagatcggg aattgatccc ccctcgacag caagcttata acttcgtata
     1081 gcatacatta tacgaagtta ttcaaccact ccaggcatag ctagagccac tgatgcatct
     1141 atagagctga agtaaagata gggttgatgc aaatcgattt agaattttca atgtagaaaa
     1201 aacaaaataa aaagtctatt cacttgatgg atagattagt caaacaaaca tagagtaggc
     1261 acttcccttg actctgtaaa cttttggatt taacagattg tgcactcctt ttagttaaac
     1321 acattagtcc ctgaaaacta agaccctaac cataatccca ttgtagaccc acctactcgg
     1381 gtgaatataa agctcgcaca gatcatctca cagacatcag accttggcaa aggagcatcc
     1441 tgaacatctc tacacactga ggcaacccta gggccgccac catgctgtgt tgtatcagaa
     1501 gaacaaagcc tgtggagaag aatgaggagg ctgaccagga actgcagagc acggtaccgc
     1561 ggtccctgta tccacctgtc gccaccatgg tgagcaaggg agaggagctg ttcacaggag
     1621 tggtgcctat cctggtggag ctggatggag atgtgaatgg acacaagttc tctgtgagag
     1681 gagagggaga gggagatgct acaaatggaa agctgacact gaagttcatc tgcacaacag
     1741 gaaagctgcc tgtgccttgg cctacactgg tgacaacact gagccatgga gtgcagtgtt
     1801 ttgctagata ccctgaccat atgaagcagc atgacttctt caagtctgct atgcctgagg
     1861 gatatgtgca ggagagaaca atcttcttca aggatgatgg aacatacaag acaagagctg
     1921 aggtgaagtt tgagggagac acactggtga acagaattga actgaaggga gtggacttca
     1981 aggaggatgg aaacatcctg ggacacaagc tggagtacaa cttcaacagc cacaacatct
     2041 acattatggc tgtgaagcag aagaatggaa tcaaggtgaa cttcaagatc agacacaatg
     2101 tggaggatgg atctgtgcag ctggctgacc actaccagca gaacacacct attggagatg
     2161 gacctgtgct gctgcctgac agccactacc tgagcacaca gtctgtgctg agcaaggacc
     2221 ctaatgagaa gagagaccat atggtgctgc tggagttcag aacagctgct ggaatcacac
     2281 tgggaatgga cgagctgtac aagtaaagcg gccgcgactc tagatcataa tcagccatac
     2341 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
     2401 acataaaatg aatgcaattg ttgttgttat cgaattctgc aggaattggg tggcatccct
     2461 gtgacccctc cccagtgcct ctcctggccc tggaagttgc cactccagtg cccaccagcc
     2521 ttgtcctaat aaaattaagt tgcatcattt tgtctgacta ggtgtccttc tataatatta
     2581 tggggtggag gggggtggta tggagcaagg ggcaagttgg gaagacaacc tgtagggcac
     2641 gagggggggc ccgaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     2701 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     2761 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     2821 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcaa ggcatgctgg
     2881 ggatgcggtg ggctctatgg tagggataac agggtaatat aaaataaaat aaaataaaat
     2941 aaaataaaat aaaataaaat aaaataaaat aaagcgcgca ctagtgatat caagcttaaa
     3001 caagaatctc tagttttctt tcttgctttt acttttactt ccttaatact caagtacaat
     3061 tttaatggag tactttttta cttttactca agtaagattc tagccagata cttttacttt
     3121 taattgagta aaattttccc taagtacttg tactttcact tgagtaaaat ttttgagtac
     3181 tttttacacc tctgctcgac catatgggag agctcccaac gcgttggatg catagcttga
     3241 gtattctata gtgtcaccta aatagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     3301 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     3361 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     3421 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     3481 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     3541 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     3601 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     3661 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     3721 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     3781 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     3841 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     3901 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     3961 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     4021 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     4081 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     4141 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     4201 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     4261 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     4321 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     4381 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     4441 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     4501 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     4561 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     4621 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     4681 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     4741 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     4801 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     4861 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     4921 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     4981 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     5041 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     5101 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     5161 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     5221 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     5281 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     5341 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     5401 ttccgcgcac atttccccga aaagtgccac ctgatgcggt gtgaaatacc gcacagatgc
     5461 gtaaggagaa aataccgcat caggaaattg taagcgttaa tattttgtta aaattcgcgt
     5521 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
     5581 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
     5641 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
     5701 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
     5761 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
     5821 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
     5881 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
     5941 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     6001 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     6061 gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa ttgtaatacg actcactata
     6121 gggcgaattg ggcccagagg tgtaaagtac ttgagtaatt ttacttgatt actgtactta
     6181 agtattattt ttggggattt ttactttact tgagtacaat taaaaatcaa tacttttact
     6241 tttacttaat tacatttttt tagaaaaaaa agtacttttt actccttaca attttattta
     6301 cagtcaaaaa gtacttattt tttggagatc acttcattct attttccctt gctattacca
     6361 aaccaattga attgcgctga tgcccagttt aatttagggt aatggccggc cataacttcg
     6421 tatagcatac attatacgaa gttatccgga gtactgtcct ccgacactag aggtcggagt
     6481 actgtcctcc gacgcaaggc ggagtactgt cctccgacgc aaggtcggag tactgtcctc
     6541 cgacactaga ggtcggagta ctgtcctccg ccatacgtag cagggtagac tgtagagggt
     6601 atataatgga acccatcgcg tctcagcctc actttgagct cctccacacg atttcaccag
     6661 gccgccacca tgctgtgttg tatcagaaga acaaagcctg tggagaagaa tgaggaggct
     6721 gaccaggaac tgcagcctgc aggaagcaca gctacaatga tggtgagcaa gggcgaggag
     6781 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     6841 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     6901 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
     6961 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     7021 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     7081 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     7141 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     7201 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
     7261 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     7321 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
     7381 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     7441 gccgggatca ctctcggcat ggacgagctg tacaagtact tgtacaaggg aagcggagct
     7501 actaacttca gcctgctgaa gcaggctgga gacgtggagg agaaccccgg gcctatgact
     7561 attgttcaag ctgcccaatc ccgctactcc accaaagcct ttgatgcttc gcgcaaattg
     7621 cctgaagaga aagtcgcggc agtgaaagag ttaatccgca tgagtgcgtc cagtgtcaac
     7681 tcgcaacctt ggcattttat tgtcgcgagc agtgaagagg gaaaagcgcg catcgccaaa
     7741 gcaacacaag gtggttttgc tgctaatgag cgcaagattt tggatgcttc tcacgttgtg
     7801 gtgttctgcg ccaaaacggc gattgatgaa gcgtacctac tcgacctttt ggaaagcgaa
     7861 gacaaagatg gccgctacgc cgatgtcgaa gcaaaaaatg gcatgcacgc tggtcgttca
     7921 tttttcgtca acatgcaccg ctttgacttg aaagacgcgc atcactggat ggaaaagcaa
     7981 gtttacctca atgtggggac gctgctattg ggtgcttctg cgatggagat cgacgcggtg
     8041 ccaattgaag gcttcgatgc caaggtgctt gatgaggagt ttggtctgcg tgagaagggc
     8101 tttaccagcg tggtgattgt gccgctgggt taccatagcg aagacgattt taatgctaag
     8161 ctgccaaaat cacgttggtc agcagagact gtttttaccg aaatctaagt cgagcacgac
     8221 cgatcctgag aacttcaggg tgagtttggg gacccttgat tgttctttct t
//
