LOCUS       sequence-310830-       11731 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tol2 vector for skeletal-muscle expression of QFGal4. Contains 
            cmlc2:mRFP-SV40pA transgenesis marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11731)
  AUTHORS   Burgess J, Burrows JT, Sadhak R, Chiang S, Weiss A, D'Amata C, 
            Molinaro AM, Zhu S, Long M, Hu C, Krause HM, Pearson BJ
  TITLE     An optimized QF-binary expression system for use in zebrafish.
  JOURNAL   Dev Biol. 2020 Jul 19. pii: S0012-1606(20)30202-5. doi: 
            10.1016/j.ydbio.2020.07.007.
  PUBMED    32697972
REFERENCE   2  (bases 1 to 11731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Biol. 
            2020 Jul 19. pii: S0012-1606(20)30202-5. doi: 
            10.1016/j.ydbio.2020.07.007."
COMMENT     Sequence Label: pTol2CG2-acta1:QFGal4-SV40pA
FEATURES             Location/Qualifiers
     source          1..11731
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(38..62)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    64..171
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     81..97
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(131..147)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(185..205)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(213..229)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    245..379
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      1477..1486
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1531..1540
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1594..1603
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1600..2277
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             1600..2277
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(1730..1748)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1998..2017
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     2192..2215
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    join(2740..2927,2928..2939)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(2973..2989)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3026..3046)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3026..3044)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3065..3081)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3078..3100)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3089..3105
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3113..3119,3120..3137,3138..3143))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3158..3179
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3296..3313)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3467..4055)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3547..3566)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4226..5017,5018..5086))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4849..4868
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5087..5191)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5354..5375)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(5828..5839,5840..6002)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     6375..6391
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6411..6431
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    10388..10412
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     10444..10460
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      10471..10480
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             11134..11478
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     polyA_signal    11508..11642
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(join(11726..11731,1..11))
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cgtcgacctc gagggggggc ccggtaccca gcttttgacc cagctttctt gtacaaagtg
       61 ggaggtaccg ggccccccct cgaggtcgac ggtatcgata agcttgatat cgaattcctg
      121 cagcccgggg gatccactag ttctagagcg gccgccaccg cggtggagct caccggtaga
      181 tctgcaactt tattatacat agttgataat tcactggccg tcgttttacg gtaccatcga
      241 tgatgatcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
      301 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
      361 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
      421 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta
      481 tgatcctcta gatcagatct tgaccaaagc ttaaatcagt tgtgttaaat aagagacatt
      541 caaaataaat gtaaatgagc tctccaaatc agcagactta acattcttta aaatgattga
      601 ttcaatagtg ataaaaatca ggcatagcca gttgtaactt tagataaatt acagaaaatg
      661 tcaaatacag agaaccgatt cttttttatg atacatccaa gcacacattt aacacaatcc
      721 aggcaaaccc cgaatttcac agtcacaagc actgtttgta caagagcttt gcctaaggac
      781 acacagtctc tataagtcca ggtcgttggt ttcactctta ttttaaacat gtgacatttt
      841 tcctgccatc ctgtcttagg ctgctgtttg cttcattcca tgtcacatta atttcctcag
      901 tagcaccttt tacacacaca gccaatcttt tccagaaaat tcaattgctt tgaagagata
      961 atgtgtgaac aaatccattt agaaaaggaa aattaagaat ttgtaaaatc atctgtaaat
     1021 tgttggcatt cttctgtata tgaacatcac atcatttaca ggtaaaggtc tggtcattaa
     1081 ttatatgaca atttactggt attattttgt gaaaggggct attttcaatg cgttcatcca
     1141 tccttttcat ccctcaaatc tctcattcac gtccccctcc ccatctgcac actttatctc
     1201 attttccacc ctgctggaat ctgagcactt gtgcagttat cagggctcct gtatttagga
     1261 ggctctgggt gtccatgtag gggacgaaca gaaacactgc agacctttat agaagaacaa
     1321 atgataagag tcctcgtaca taaagactcc attagtaacg tcagtgaccc aggagcccag
     1381 accaacagca aagcagacag tgaaatcact agtgaattcg cggccgcctg caggtcgacg
     1441 gtatcgattc gaattcaagg cctctcgagc ctctaagcca ccatgggctg catcaagagc
     1501 aagcgcaagg acaacctgaa cgacgacgag gccgccatgg gctgcatcaa gagcaagcgc
     1561 aaggacaacc tgaacgacga cgaggcaccg gtcgccacca tggcctcctc cgaggacgtc
     1621 atcaaggagt tcatgcgctt caaggtgcgc atggagggct ccgtgaacgg ccacgagttc
     1681 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag
     1741 gtgaccaagg gcggccccct gcccttcgcc tgggacatcc tgtcccctca gttccagtac
     1801 ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc
     1861 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg
     1921 acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc
     1981 aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctccacc
     2041 gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagatgag gctgaagctg
     2101 aaggacggcg gccactacga cgccgaggtc aagaccacct acatggccaa gaagcccgtg
     2161 cagctgcccg gcgcctacaa gaccgacatc aagctggaca tcacctccca caacgaggac
     2221 tacaccatcg tggaacagta cgagcgcgcc gagggccgcc actccaccgg cgcctaatac
     2281 gtagatccag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
     2341 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     2401 agctgcaata aacaagagat ctgcgaagat acggccacgg gtgctcttga tcctgtggct
     2461 gattttggac tgtgctgctc gcagctgctg atgaatcaca tacttcctcc attttcttcc
     2521 actgattgac tgttataatt tccctaattt ccaggtcaag gtgctgtgca ttgtggtaat
     2581 agatgtgaca tgacgtcact tccaaaggac caatgaacat gtctgaccaa tttcatataa
     2641 tgtgaaaacg attttcatag gcagaataaa taacatttaa attaaactgg gcatcagcgc
     2701 aattcaattg gtttggtaat agcaagggaa aatagaatga agtgatctcc aaaaaataag
     2761 tactttttga ctgtaaataa aattgtaagg agtaaaaagt actttttttt ctaaaaaaat
     2821 gtaattaagt aaaagtaaaa gtattgattt ttaattgtac tcaagtaaag taaaaatccc
     2881 caaaaataat acttaagtac agtaatcaag taaaattact caagtacttt acacctctgg
     2941 ttcttgaccc cctaccttca gcaagcccag cagatccact agttctagag cggccgccac
     3001 cgcggtggag ctccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat
     3061 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     3121 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     3181 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     3241 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     3301 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     3361 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     3421 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     3481 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     3541 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     3601 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     3661 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     3721 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     3781 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     3841 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     3901 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     3961 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     4021 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     4081 ggtctgacgc tcagtggaac gaaaactcac gttaaggcat tttggtcatg agattatcaa
     4141 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     4201 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     4261 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     4321 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     4381 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     4441 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     4501 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     4561 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     4621 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     4681 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     4741 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     4801 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     4861 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     4921 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     4981 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     5041 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     5101 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     5161 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat
     5221 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     5281 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     5341 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     5401 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     5461 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     5521 atttagagct tgacggggaa aggctggcta agaactcatc agcctccccg gtccatctac
     5581 ccacgtacca atgcaccaat tggccacaat gacggctact acatggtgcc attccttcct
     5641 ctttatagga atggagacta cctcctgtcc aacaatgctc ttggatacga gtacgcctac
     5701 ctgttggacc caggtcattg cacaacacca gaaatgccct ctgatctgca aaagacgtga
     5761 atatctgttc agacacccat atccactctg ttccacacag gtcagaggtt tgtccaggag
     5821 ttcttgacag aggtgtaaaa agtactcaaa aattttactc aagtgaaagt acaagtactt
     5881 agggaaaatt ttactcaatt aaaagtaaaa gtatctggct agaatcttac ttgagtaaaa
     5941 gtaaaaaagt actccattaa aattgtactt gagtattaag gaagtaaaag taaaagcaag
     6001 aaagaaaact agagattctt gtttaagctt ttaatctcaa aaaacattaa atgaaatgca
     6061 tacaaggttt tatcctgctt tagaactgtt tgtatttaat tatcaaacta taagacagac
     6121 aatctaatgc cagtacacgc tactcaaagt tgtaaaacct cagatttaac ttcagtagaa
     6181 gctgattctc aaaattgtta gtgtcaagcc tagctctttt ggggctgaaa agcaatcctg
     6241 cagtgctgaa aagcctctca caggcagccg atgcgggaag aggtgtatta gtcttgatag
     6301 agaggctgca aatagcagga aacgtgagca gagactccct ggtgtctgaa acacaggcca
     6361 gatgggccct cgagcaggaa acagctatga ccatgattac gccaagctat caactttgta
     6421 tagaaaagtt ggcacgcgca gattccgtgt gggcctggta atatcttacc attttaagct
     6481 attttaagat tagggaaaaa aattaaataa aactacttta aaattacttt ccttaaaaag
     6541 caacatattt atttagtttt tgtttaactg gctcaagatt gtaatatttt acttttacat
     6601 gtttttttaa gtacctaaat gtgttcaaaa gtaaagtatg ttattgtttt ttttttttaa
     6661 tgttagctaa gctttaaatt gtaatatatt acttttaaaa gtgacttttt aaaaatgtaa
     6721 ataaaaaagt aactataaat gtgtaatgca tgttatttct gtgggttttt aattttcatc
     6781 tcaactgggc ttacaatttt aacataataa aaatcaaatc aaatcaaatc aggcccttat
     6841 ttgtcacata cactcttgta tactgaaata aactaaatgt tgagaaaaat ttaaatacac
     6901 caaaataacg catacctttt ttgtaattac ctaagtaaaa catttagttc cttttttaag
     6961 tgactcaaga ttgtaatcta ttaattgtaa aagataaaac tagtgatcaa tctgacgtgc
     7021 ttctatgcag cctgtgagat taattgtgat gtttaattat taaacaattt aaatggatca
     7081 aacatttcac atgtgcttat tgtgtccaac aaaaccagca gaagcactta aaaatattgc
     7141 atgacaacat gagtttttaa tgtaaattat atatacttcc taaacgaagt taatcatatt
     7201 tctaaaataa taatgtcatt gaaatattta aataaatgat aagctttagt tgattcaaca
     7261 taaaattgaa agcaataagg actttttttt tttacagcgg tgaagcaaaa gcatgtaata
     7321 atcatataat ataataaata aataaataaa taatggctct tggtgcaaat atgaacactt
     7381 taaataaata tagaataaat atgatcaata agtcataacg atgtgtttta tgttgattat
     7441 aatttaaaca tgtttctaac gcaagtttta aaaactgttt taaagtatta ttttaagtta
     7501 taagatcaat ttgactcaac attttgtagt gaaaatatgt tgtgtaaaat taatataata
     7561 tacactataa aaatgcaggg ttccacacaa tacattaatg ttgtcccaac acaaatcgat
     7621 taagttaaca aatatatgtg gattgaacat aaaaaattaa gttgtcccaa tgaaatctca
     7681 agaattgtgt tgtttcagct cattttaaat aagtagtttt aacaagtaaa aaataacata
     7741 tttttgagtg tgtatatata tatattacaa aatataataa tataaaatgt aaaagatata
     7801 aaccatgcta catatattta agagctattt tgcacattac ttttttgcaa gccattttag
     7861 tgtttttttt acaaatatat attagctaaa tatacaatat cttaaatata caattaatca
     7921 gacatgcaat taatgcattt aacctacata aaaatacatg atcaacatat gttttatgct
     7981 gattacaact aaatcaaacg taatgaaatt aatttataaa agcgatttaa atatgtcaat
     8041 attttaagtt aaaatcactt gaaacctact ataaatgaac ttaaattaaa aaaatctcct
     8101 ttaattccaa tgcaacagtg ttgttttttc agctgaggac ccccggtgca gatggttggc
     8161 ccccggcccg ctattctaag gaagcctttc ttaattttag ggcggcgctg catgcgctca
     8221 gatcagattc acgggtggag agggttattt tctctttttt attttttctt gcctgctcat
     8281 atttgggcct gtgtgaaccc caggctccgc ctcggccccc aggagccgct cttatttaaa
     8341 ggggatcctc gccttcagtc ttcgtcccag tggaagcatc agtcttgtga taacaacttg
     8401 gtgagtccta cagaccagcg ctcgactcta ggctttaaat gcattgattt ctctggatta
     8461 gctaccggga gagttttaac tttgcttatt agtcttgtgc tcgaactgct aggtttaaag
     8521 agtgttttct ctaatgcatg catgaagcga cagctgaaaa ccagaaatag agcgtttgtc
     8581 ctcctgttaa actctagcct ttctttggca tttgtcagtc cgagtcttca gctgccggca
     8641 ggagattggg tttcagtaac acttgctgag cacaaactac acgtgctttc ttcagattta
     8701 attcattatt aatcattcta gatttaaatg atgttcttac tcattgctga ttattttagc
     8761 cttgcggagc agattaaatg agcacgtgtt tcattaagat tgatttaaat ataattaata
     8821 tttcaccact gtagttttaa aattgccttc taagtcatgg ttgattttta ataggtatcg
     8881 gctctaatta gattactatt ttggttttta aatgttattt ctatttcatt tgtaggctcg
     8941 tttttaatgt gattttatta gattctagtt ttaaaatcat ctcttagcct taatgagcag
     9001 actgaatgaa catgtttcat taagattaac ttcattataa tgaattattt aatcattcta
     9061 gtgggttata aatcagctct atttaaatta attgtcgtgt ttatttctat ttaatttttg
     9121 attatttttt cagggtcgtt tttatgtgat tttttcccgt ttctaatttt aaactaattg
     9181 tttgattttt atatgattcg actcttttta aatgaattgt tttgcttttt ttattgagca
     9241 tatatatatt atacaatttc aatcaacaat tgttgtgctt tttaaatgtt gtttctattt
     9301 catttctagg gtcgatttta ttcgattcta gttttaaaat cgtgattttt aacttctaac
     9361 cttactgagc agactgaatg aacatgtttc atcaagatta acttcattat agttaattat
     9421 tgaatcgtgc tagtggttta taaatcagct ctatttaaat taatcatcat cattatttct
     9481 atttaatttt tattatttct ttaggattat ttttatgtga tttttttcac gttctagttt
     9541 taaactcaat gctcattact gctcaccctt gctgagcagt ctttcgttaa gattaaatta
     9601 attaataacc attctagttt taaaacagtg tcttaaggca tagctgatat tttacaagtt
     9661 tactatttaa tatatctcct ttcttttttt agggtcgatt tcatctgatt tgattaattt
     9721 ttattatctt cactaagctt tttgttctag gttttattct cagtattatt actgttatat
     9781 ttgaactgaa tttctcctca tagtttattc attttattta tttatttttt gtttattcgc
     9841 tattttgtat tttagttttt aattagaaat tttattagaa tcgttttgaa ctagttttta
     9901 tgcactctaa cattaatatt aaatcataac tattattttt attataattt atttgtgttt
     9961 atctttttgt gtagctttca tgtgttttta ttttagtttt attatgtttg cattgaattt
    10021 tttggactag tttatataac gatatacaga aaagccatga caacaaatgt ttttataata
    10081 cctattaagt ataagctaat cagatttcca ctattctgtt caaagttaac attattagcc
    10141 attctgatgg atcatttgac atttgtcata tgtctttatg aacactgctc tttcagatgc
    10201 cacttttaaa gctgtttttt acagtaaatg aacgtttatt attatgacat tatttcctaa
    10261 ggcctctaga gtacagcagg atgtggcgtg tgcattaccg tgtaattatg ctgactgtaa
    10321 atgtgcattt atagactgtc agcatgtctg actgtgcgct gttgtgattg gtcctgcaca
    10381 gaccaaggca agtttgtaca aaaaagcagg ctcgaattgg agctccaccg cggtggcggc
    10441 cgctctagaa ctagtggatc ccccggtgcc gccaccatgg cacccaagaa gaagaggaag
    10501 atgccgccta aacgcaagac actcaatgcc gctgccgaag ccaatgccca cgctgatggc
    10561 catgctgatg gcaatgctga tggtcatgtc gctaacactg cagcaagcag caacaacgcc
    10621 cgttttgcgg acttgaccaa cattgacaca cccggcctcg gccctaccac gacgacgtta
    10681 cttgtcgagc ccgctcgttc gaaacgccag agagtctcga gggcctgtga tcagtgtcga
    10741 gctgcacgtg aaaagtgtga tggaatccag ccggcttgct tcccctgtgt gtcgcagggc
    10801 cggtcgtgta cctaccaggc cagtcccaag aagcgaggag tccagacggg ctacatccgc
    10861 actctcgaac tggctctggc ttggatgttc gagaacgttg cccgcagcga ggacgccctc
    10921 cacaatcttt tggtccgtga tgctggccag ggcagcgctc tcctggtcgg caaagactcg
    10981 cctgctgcag aacgcctgca tgcaagatgg gcgacgagtc gagtcaacaa aagcatcacc
    11041 cgtcttctct caggtgctag cactagtagc gccggtcaac agcaacagct gcaatcatta
    11101 gtgccactga ccccgtctgc tttgtttggt ggcgccaatt ttaatcaaag tgggaatatt
    11161 gctgatagct cattgtcctt cactttcact aacagtagca acggtccgaa cctcataaca
    11221 actcaaacaa attctcaagc gctttcacaa ccaattgcct cctctaacgt tcatgataac
    11281 ttcatgaata atgaaatcac ggctagtaaa attgatgatg gtaataattc aaaaccactg
    11341 tcacctggtt ggacggacca aactgcgtat aacgcgtttg gaatcactac agggatgttt
    11401 aataccacta caatggatga tgtatataac tatctattcg atgatgaaga taccccacca
    11461 aacccaaaaa aagagtaaga attcactata gtgagtcgta ttacgtagat ccagacatga
    11521 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
    11581 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
    11641 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
    11701 tttaattcgc ggccatcaag cttatcgata c
//
