LOCUS       sequence-310833-        9294 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tol2 vector for heat-shock inducible expression of QFGal4. Contains 
            cmlc2:mRFP-SV40pA transgenesis marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Burgess J, Burrows JT, Sadhak R, Chiang S, Weiss A, D'Amata C, 
            Molinaro AM, Zhu S, Long M, Hu C, Krause HM, Pearson BJ
  TITLE     An optimized QF-binary expression system for use in zebrafish.
  JOURNAL   Dev Biol. 2020 Jul 19. pii: S0012-1606(20)30202-5. doi: 
            10.1016/j.ydbio.2020.07.007.
  PUBMED    32697972
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Biol. 
            2020 Jul 19. pii: S0012-1606(20)30202-5. doi: 
            10.1016/j.ydbio.2020.07.007."
COMMENT     Sequence Label: pTol2CG2-hsp70I:QFGal4-SV40pA
FEATURES             Location/Qualifiers
     source          1..9294
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    25..49
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     76..92
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    176..310
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(340..684)
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     regulatory      1338..1347
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1358..1374)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1406..1430)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2950..2970)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(2990..3006)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    join(3379..3541,3542..3553)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     4006..4027
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4190..4294
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4295..4363,4364..5155)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4513..4532)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5326..5914
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5815..5834
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6068..6085
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6202..6223
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6238..6243,6244..6261,6262..6268)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6276..6292
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6281..6303
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6300..6316
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6335..6355
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6337..6355
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     6392..6408
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    join(6442..6453,6454..6641)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     CDS             complement(7104..7781)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             complement(7104..7781)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(7166..7189)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(7364..7383)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     7633..7651
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      7778..7787
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      7841..7850
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      7895..7904
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    9002..9136
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     9152..9168
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9176..9196
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    join(9210..9294,1..23)
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     9234..9250
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(join(9284..9294,1..6))
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acctcgaggg ggggcccggt acctcccact ttgtacaaga aagctgggtc aaaagctggg
       61 taccgggccc cccctcgagg tcgacggtat cgataagctt gatggccgcg aattaaaaaa
      121 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
      181 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
      241 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
      301 tgtctggatc tacgtaatac gactcactat agtgaattct tactcttttt ttgggtttgg
      361 tggggtatct tcatcatcga atagatagtt atatacatca tccattgtag tggtattaaa
      421 catccctgta gtgattccaa acgcgttata cgcagtttgg tccgtccaac caggtgacag
      481 tggttttgaa ttattaccat catcaatttt actagccgtg atttcattat tcatgaagtt
      541 atcatgaacg ttagaggagg caattggttg tgaaagcgct tgagaatttg tttgagttgt
      601 tatgaggttc ggaccgttgc tactgttagt gaaagtgaag gacaatgagc tatcagcaat
      661 attcccactt tgattaaaat tggcgccacc aaacaaagca gacggggtca gtggcactaa
      721 tgattgcagc tgttgctgtt gaccggcgct actagtgcta gcacctgaga gaagacgggt
      781 gatgcttttg ttgactcgac tcgtcgccca tcttgcatgc aggcgttctg cagcaggcga
      841 gtctttgccg accaggagag cgctgccctg gccagcatca cggaccaaaa gattgtggag
      901 ggcgtcctcg ctgcgggcaa cgttctcgaa catccaagcc agagccagtt cgagagtgcg
      961 gatgtagccc gtctggactc ctcgcttctt gggactggcc tggtaggtac acgaccggcc
     1021 ctgcgacaca caggggaagc aagccggctg gattccatca cacttttcac gtgcagctcg
     1081 acactgatca caggccctcg agactctctg gcgtttcgaa cgagcgggct cgacaagtaa
     1141 cgtcgtcgtg gtagggccga ggccgggtgt gtcaatgttg gtcaagtccg caaaacgggc
     1201 gttgttgctg cttgctgcag tgttagcgac atgaccatca gcattgccat cagcatggcc
     1261 atcagcgtgg gcattggctt cggcagcggc attgagtgtc ttgcgtttag gcggcatctt
     1321 cctcttcttc ttgggtgcca tggtggcggc accgggggat ccactagttc tagagcggcc
     1381 gccaccgcgg tggagctcca attcgagcct gcttttttgt acaaacttgg caggaaaaaa
     1441 aaacaattag aattaatttt atatttatac aatttatggt gcaattgttt cattatgaaa
     1501 ggatttttct agtcaacaag tgaaattctc ttaataacca agttattatt agttgtacaa
     1561 gtaaaacgct aattttcttt tgtagacaac attaacacaa attcatttag gacatttgct
     1621 agtatttatg gttattatgc tcatataaaa taatgtcagc aacataaagt aaatgcccgt
     1681 ctgttcattg ctttttttaa aaatccttat tgtgaaaacg ggaaatataa ttttctagta
     1741 gacctaaaat aagatgaaca taggcagtcc acatgtaaaa agattacata tacaaagttg
     1801 attttttgac attaagtaca tataatctct atataattct gattagccaa tagagtacaa
     1861 tatattgaga cttaccttac agtagattct taaatagagc gaataaacac ctcgtcgggg
     1921 aaaaagtcct gtcaggctgc tcttttcttc tttgtcaagt cgcttctctt cggttgagtt
     1981 tctctgcggt ctctctttcg ccctttaaat acagcagagc gcgcacccgt tcagactcta
     2041 cactgttctg gaagcctcca gtgaattcga ccaatcaaaa cacagcaggc agccgcgtca
     2101 cttcttcagc tgtcattgag aggagccgct gagaggaatg tagtggagat ttccagagtt
     2161 ttaccgtgaa attacgcgaa acagcggtca aaaatacaaa gagatttaaa atgttttttt
     2221 tatttgagtg ttttattgta cttattgctg aaatatgtgc ggttgtgtga tgtgttgttt
     2281 ttccagttgc aatgaatgaa caaactaaaa tgataaaatg tttgaacgat tcagtaaaaa
     2341 aaaatgcaat aaaaaaatct tgctgaaatg ttttacacag tagcaaaatc gggcctgtgg
     2401 tgttttcagc tacatagaag gcaaacctgt tttatataag ctaatagctt tggtcagatt
     2461 tgtttaacgt ttcaacaaaa caaatggatg tttactgtac tatattgatt ggttaaaacc
     2521 aatactttat tctgggattg cagtcttaac tatttttgta gtgttaaaac caacttgcta
     2581 acttgagagg tcatgtttac cctcagagaa ataggcacaa gtctctttgt ttaatattgg
     2641 agatcatcct ccttgttcag tcgtggtctg tatttaattt taatgtatat gattgccgta
     2701 aaagtgttag aaagtttacc ctggtgctat gaaatcaatc tgctgctttt gacccagtta
     2761 gtgtgtcttt aatctaactt aatgagaccc cagtgtggtc aacaatcaat agaaacaaag
     2821 tgtgttgttt tataactgtt aactcctatt attttttatc ttctgtagca tatagacaaa
     2881 acatgctcgt tctaatagtt taataaaatt aatttgattt ttggaaataa ccaagcgaca
     2941 cccctgaagc aacttttcta tacaaagttg atagcttggc gtaatcatgg tcatagctgt
     3001 ttcctgctcg agggcccatc tggcctgtgt ttcagacacc agggagtctc tgctcacgtt
     3061 tcctgctatt tgcagcctct ctatcaagac taatacacct cttcccgcat cggctgcctg
     3121 tgagaggctt ttcagcactg caggattgct tttcagcccc aaaagagcta ggcttgacac
     3181 taacaatttt gagaatcagc ttctactgaa gttaaatctg aggttttaca actttgagta
     3241 gcgtgtactg gcattagatt gtctgtctta tagtttgata attaaataca aacagttcta
     3301 aagcaggata aaaccttgta tgcatttcat ttaatgtttt ttgagattaa aagcttaaac
     3361 aagaatctct agttttcttt cttgctttta cttttacttc cttaatactc aagtacaatt
     3421 ttaatggagt acttttttac ttttactcaa gtaagattct agccagatac ttttactttt
     3481 aattgagtaa aattttccct aagtacttgt actttcactt gagtaaaatt tttgagtact
     3541 ttttacacct ctgtcaagaa ctcctggaca aacctctgac ctgtgtggaa cagagtggat
     3601 atgggtgtct gaacagatat tcacgtcttt tgcagatcag agggcatttc tggtgttgtg
     3661 caatgacctg ggtccaacag gtaggcgtac tcgtatccaa gagcattgtt ggacaggagg
     3721 tagtctccat tcctataaag aggaaggaat ggcaccatgt agtagccgtc attgtggcca
     3781 attggtgcat tggtacgtgg gtagatggac cggggaggct gatgagttct tagccagcct
     3841 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     3901 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     3961 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     4021 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     4081 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     4141 acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     4201 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     4261 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     4321 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     4381 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     4441 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     4501 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     4561 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     4621 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     4681 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     4741 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     4801 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     4861 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     4921 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     4981 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     5041 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     5101 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     5161 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     5221 gatctaggtg aagatccttt ttgataatct catgaccaaa atgccttaac gtgagttttc
     5281 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     5341 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     5401 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     5461 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     5521 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     5581 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     5641 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     5701 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     5761 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     5821 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     5881 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     5941 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     6001 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     6061 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     6121 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     6181 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     6241 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     6301 aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca
     6361 aaagctggag ctccaccgcg gtggcggccg ctctagaact agtggatctg ctgggcttgc
     6421 tgaaggtagg gggtcaagaa ccagaggtgt aaagtacttg agtaatttta cttgattact
     6481 gtacttaagt attatttttg gggattttta ctttacttga gtacaattaa aaatcaatac
     6541 ttttactttt acttaattac atttttttag aaaaaaaagt actttttact ccttacaatt
     6601 ttatttacag tcaaaaagta cttatttttt ggagatcact tcattctatt ttcccttgct
     6661 attaccaaac caattgaatt gcgctgatgc ccagtttaat ttaaatgtta tttattctgc
     6721 ctatgaaaat cgttttcaca ttatatgaaa ttggtcagac atgttcattg gtcctttgga
     6781 agtgacgtca tgtcacatct attaccacaa tgcacagcac cttgacctgg aaattaggga
     6841 aattataaca gtcaatcagt ggaagaaaat ggaggaagta tgtgattcat cagcagctgc
     6901 gagcagcaca gtccaaaatc agccacagga tcaagagcac ccgtggccgt atcttcgcag
     6961 atctcttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     7021 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     7081 cttatcatgt ctggatctac gtattaggcg ccggtggagt ggcggccctc ggcgcgctcg
     7141 tactgttcca cgatggtgta gtcctcgttg tgggaggtga tgtccagctt gatgtcggtc
     7201 ttgtaggcgc cgggcagctg cacgggcttc ttggccatgt aggtggtctt gacctcggcg
     7261 tcgtagtggc cgccgtcctt cagcttcagc ctcatcttga tctcgccctt cagggcgccg
     7321 tcctcggggt acatccgctc ggtggaggcc tcccagccca tggtcttctt ctgcattacg
     7381 gggccgtcgg aggggaagtt ggtgccgcgc agcttcacct tgtagatgaa ctcgccgtcc
     7441 tgcagggagg agtcctgggt cacggtcacc acgccgccgt cctcgaagtt catcacgcgc
     7501 tcccacttga agccctcggg gaaggacagc ttcaagtagt cggggatgtc ggcggggtgc
     7561 ttcacgtagg ccttggagcc gtactggaac tgaggggaca ggatgtccca ggcgaagggc
     7621 agggggccgc ccttggtcac cttcagcttg gcggtctggg tgccctcgta ggggcggccc
     7681 tcgccctcgc cctcgatctc gaactcgtgg ccgttcacgg agccctccat gcgcaccttg
     7741 aagcgcatga actccttgat gacgtcctcg gaggaggcca tggtggcgac cggtgcctcg
     7801 tcgtcgttca ggttgtcctt gcgcttgctc ttgatgcagc ccatggcggc ctcgtcgtcg
     7861 ttcaggttgt ccttgcgctt gctcttgatg cagcccatgg tggcttagag gctcgagagg
     7921 ccttgaattc gaatcgatac cgtcgacctg caggcggccg cgaattcact agtgatttca
     7981 ctgtctgctt tgctgttggt ctgggctcct gggtcactga cgttactaat ggagtcttta
     8041 tgtacgagga ctcttatcat ttgttcttct ataaaggtct gcagtgtttc tgttcgtccc
     8101 ctacatggac acccagagcc tcctaaatac aggagccctg ataactgcac aagtgctcag
     8161 attccagcag ggtggaaaat gagataaagt gtgcagatgg ggagggggac gtgaatgaga
     8221 gatttgaggg atgaaaagga tggatgaacg cattgaaaat agcccctttc acaaaataat
     8281 accagtaaat tgtcatataa ttaatgacca gacctttacc tgtaaatgat gtgatgttca
     8341 tatacagaag aatgccaaca atttacagat gattttacaa attcttaatt ttccttttct
     8401 aaatggattt gttcacacat tatctcttca aagcaattga attttctgga aaagattggc
     8461 tgtgtgtgta aaaggtgcta ctgaggaaat taatgtgaca tggaatgaag caaacagcag
     8521 cctaagacag gatggcagga aaaatgtcac atgtttaaaa taagagtgaa accaacgacc
     8581 tggacttata gagactgtgt gtccttaggc aaagctcttg tacaaacagt gcttgtgact
     8641 gtgaaattcg gggtttgcct ggattgtgtt aaatgtgtgc ttggatgtat cataaaaaag
     8701 aatcggttct ctgtatttga cattttctgt aatttatcta aagttacaac tggctatgcc
     8761 tgatttttat cactattgaa tcaatcattt taaagaatgt taagtctgct gatttggaga
     8821 gctcatttac atttattttg aatgtctctt atttaacaca actgatttaa gctttggtca
     8881 agatctgatc tagaggatca taatcagcca taccacattt gtagaggttt tacttgcttt
     8941 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
     9001 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     9061 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     9121 ttatcatgtc tggatcatca tcgatggtac cgtaaaacga cggccagtga attatcaact
     9181 atgtataata aagttgcaga tctaccggtg agctccaccg cggtggcggc cgctctagaa
     9241 ctagtggatc ccccgggctg caggaattcg atatcaagct tatcgatacc gtcg
//
