LOCUS       sequence-310838-        2880 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  5' entry vector containing QUAS5x promoter element..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Burgess J, Burrows JT, Sadhak R, Chiang S, Weiss A, D'Amata C, 
            Molinaro AM, Zhu S, Long M, Hu C, Krause HM, Pearson BJ
  TITLE     An optimized QF-binary expression system for use in zebrafish.
  JOURNAL   Dev Biol. 2020 Jul 19. pii: S0012-1606(20)30202-5. doi: 
            10.1016/j.ydbio.2020.07.007.
  PUBMED    32697972
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Biol. 
            2020 Jul 19. pii: S0012-1606(20)30202-5. doi: 
            10.1016/j.ydbio.2020.07.007."
COMMENT     Sequence Label: p5E-QUAS5x
FEATURES             Location/Qualifiers
     source          1..2880
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(211..306)
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(346..363)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(346..362)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(355..377)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      426..512
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      604..631
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(790..807)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(961..1549)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1041..1060)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1696..2505)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2419..2438
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2497..2516
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     2618..2634
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2618..2634
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2639..2658
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2639..2657
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(2730..2853)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gtgtggagga gctcaaagtg aggctgagac gcgatgggat ccattatata ccctctagag
       61 tcgacccttg cgaggataat tgtttatcct tgaggataat tgtttatcct tgaggataat
      121 tgtttatcct tgaggataat tgtttatcct tgaggataat tgtttatcct tgcggccgcc
      181 accgcggtgg agctcggtac ccagcttttg caacttttct atacaaagtt ggcattataa
      241 aaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca
      301 ttatttggag ctccatggta gcgttaactc gggcccgagc ttaagactgg ccgtcgtttt
      361 acaacgtcgt gactgggaaa acatccatgc tagcgttaac gcgagagtag ggaactgcca
      421 ggcatcaaat aaaacgaaag gctcagtcgg aagactgggc ctttcgtttt atctgttgtt
      481 tgtcggtgaa cgctctcctg agtaggacaa atccgccggg agcggatttg aacgttgtga
      541 agcaacggcc cggagggtgg cgggcaggac gcccgccata aactgccagg catcaaacta
      601 agcagaaggc catcctgacg gatggccttt ttgcgtttct acaaactctt cctggctagc
      661 ggtacgcgta ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
      721 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
      781 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
      841 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
      901 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
      961 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     1021 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     1081 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     1141 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     1201 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     1261 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     1321 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     1381 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     1441 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     1501 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     1561 gatcttttct acggggtctg acgctcagtg gaacgacgcg taactcacgt taagggattt
     1621 tggtcatgag cttgcgccgt cccgtcaagt cagcgtaatg ctctgccagt gttacaacca
     1681 attaaccaat tctgattaga aaaactcatc gagcatcaaa tgaaactgca atttattcat
     1741 atcaggatta tcaataccat atttttgaaa aagccgtttc tgtaatgaag gagaaaactc
     1801 accgaggcag ttccatagga tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc
     1861 aacatcaata caacctatta atttcccctc gtcaaaaata aggttatcaa gtgagaaatc
     1921 accatgagtg acgactgaat ccggtgagaa tggcaaaagt ttatgcattt ctttccagac
     1981 ttgttcaaca ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt
     2041 attcattcgt gattgcgcct gagcgagacg aaatacgcga tcgctgttaa aaggacaatt
     2101 acaaacagga atcgaatgca accggcgcag gaacactgcc agcgcatcaa caatattttc
     2161 acctgaatca ggatattctt ctaatacctg gaatgctgtt tttccgggga tcgcagtggt
     2221 gagtaaccat gcatcatcag gagtacggat aaaatgcttg atggtcggaa gaggcataaa
     2281 ttccgtcagc cagtttagtc tgaccatctc atctgtaaca tcattggcaa cgctaccttt
     2341 gccatgtttc agaaacaact ctggcgcatc gggcttccca tacaagcgat agattgtcgc
     2401 acctgattgc ccgacattat cgcgagccca tttataccca tataaatcag catccatgtt
     2461 ggaatttaat cgcggcctcg acgtttcccg ttgaatatgg ctcataacac cccttgtatt
     2521 actgtttatg taagcagaca gttttattgt tcatgatgat atatttttat cttgtgcaat
     2581 gtaacatcag agattttgag acacgggcca gagctgccag gaaacagcta tgaccatgta
     2641 atacgactca ctatagggga tatcgcggcc gccctgcagc tctagagctc gaattctaca
     2701 ggtcactaat accatctaag tagttggttc atagtgactg catatgttgt gttttacagt
     2761 attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt
     2821 acgtttctcg ttcaactttt ttgtacaaac ttggacgcgt cttcgaggtc gagggaattc
//
