LOCUS       sequence-31084-e         878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Kim JS, Lee C, Bonifant CL, Ressom H, Waldman T
  TITLE     Activation of p53-dependent growth suppression in human cells by 
            mutations in PTEN or PIK3CA.
  JOURNAL   Mol Cell Biol. 2007 Jan . 27(2):662-77.
  PUBMED    17060456
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2007 Jan . 27(2):662-77."
COMMENT     Sequence Label: pLP-LNCX-PIK3CA-WT
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(46..79)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             96..119
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tagaagacac cgggaccgat ccagcctccg cggccccaag cttcgataac ttcgtatagc
       61 atacattata cgaagttatc agtcgaccca ccatggatta caaggatgac gacgataagc
      121 ctccaagacc atcatcaggt gaactgtggg gcatccactt gatgccccca agaatcctag
      181 tagaatgttt actaccaaat ggaatgatag tgactttaga atgcctccgt gaggctacat
      241 taataaccat aaagcatgaa ctatttaaag aagcaagaaa ataccccctc catcaacttc
      301 ttcaagatga atcttcttac attttcgtaa gtgttactca agaagcagaa agggaagaat
      361 tttttgatga aacaagacga ctttgtgacc ttcggctttt tcaacccttt ttaaaagtaa
      421 ttgaaccagt aggcaaccgt gaagaaaaga tcctcaatcg agaaattggt tttgctatcg
      481 gcatgccagt gtgtgaattt gatatggtta aagatccaga agtacaggac ttccgaagaa
      541 atattctgaa cgtttgtaaa gaagctgtgg atcttaggga cctcaattca cctcatagta
      601 gagcaatgta tgtctatcct ccaaatgtag aatcttcacc agaattgcca aagcacatat
      661 ataataaatt agataaaggg caaataatag tggtgatctg ggtaatagtt tctccaaata
      721 atgacaagca gaagtatact ctgaaaatca accatgactg tgtaccagaa caagtaattg
      781 ctgaagcaat caggaaaaaa actcgaagta tgttgctatc ctctgaacaa ctaaaactct
      841 gtgttttaga atatcagggc aagtatattt taaaagtg
//
