LOCUS       sequence-310854-        2314 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid expresses Fzd1 under the activation of tTA 
            trans-activator along with fluorescent marker tdTomato..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Wu Y, Ma L, Qiu Q, Xu W, Misra A, Duyck K, Blanck J, Scott AR, Chen 
            S, Hassan H, Corbin TJ, Moran A, Hall K, Li H, Perera A, Yu CR
  TITLE     Molecular Control of Circuit Plasticity and the Permanence of 
            Imprinted Odor Memory
  JOURNAL   bioRxiv 2022
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2022"
COMMENT     Sequence Label: tetO-Fzd1-IRES-tdTomato (TET006)
FEATURES             Location/Qualifiers
     source          1..2314
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    join(20..38,39..61,62..80,81..103,104..122,123..145,
                     146..164,165..187,188..206,207..229,230..248,249..271,
                     272..290)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        323..361
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     358..382
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          516..745
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     728..747
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
ORIGIN
        1 gatccctcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca
       61 ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga
      121 gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt
      181 ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt
      241 gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag
      301 tcgagctcgg tacccgggtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc
      361 tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag
      421 aagacaccgg gaccgatcca gcctccgcgg ccccgaattc accacgtggc cgcatcgatt
      481 ctagaattcg ctgtctgcga gggccagctg ttggggtgag tactccctct caaaagcggg
      541 catgacttct gcgctaagat tgtcagtttc caaaaacgag gaggatttga tattcacctg
      601 gcccgcggtg atgcctttga gggtggccgc gtccatctgg tcagaaaaga caatcttttt
      661 gttgtcaagc ttgaggtgtg gcaggcttga gatctggcca tacacttgag tgacaatgac
      721 atccactttg cctttctctc cacaggtgtc cactcccagg tccaactgca gcccaagcgg
      781 atctacgcgt atggctgagg aggcggcgcc tagcgagtcc cgggccgccg gccggctgag
      841 cttggaactt tgtgccgaag cactcccggg ccggcgggag gaggtggggc acgaggacac
      901 ggccagccac cgccgccccc gggctgatcc ccggcgttgg gctagcgggc tgctgctgct
      961 gctttggttg ctggaggctc ctctgctttt gggggtccga gcgcaggcgg cgggccaggt
     1021 atccgggccg ggccagcaag ccccgccgcc gccccagccc cagcagagcg ggcagcagta
     1081 caacggcgaa cggggcatct ccatcccgga ccacggctac tgccagccca tctccatccc
     1141 gctgtgcacg gacatcgcgt acaaccagac catcatgccc aacctgctgg gccacacgaa
     1201 tcaggaggac gccggtctgg aggtgcacca gttctaccct ctggtgaagg tgcagtgctc
     1261 cgccgagctc aagttcttcc tgtgctccat gtacgcgcct gtgtgcaccg tactggagca
     1321 ggcgctaccg ccctgccgct ccctgtgcga gcgcgcacgc cagggctgcg aggcgctcat
     1381 gaacaagttc ggcttccagt ggccagacac actcaagtgc gagaagttcc cggtgcacgg
     1441 cgcaggagag ctgtgcgtgg gccagaacac gtccgacaaa ggcaccccaa ctccctcctt
     1501 gctaccagag ttctggacca gtaatccgca gcacggcggc ggtggttacc gcggcggcta
     1561 cccggggggt gccgggacgg tggagcgggg aaagttctcc tgcccgcgcg ccctcagggt
     1621 gccctcctac ctcaactacc actttctggg ggagaaggac tgcggcgcac cctgcgaacc
     1681 caccaaggtt tacgggctca tgtacttcgg gccagaggag ctgcgcttct cgcgcacctg
     1741 gataggcatc tggtccgtgc tgtgctgcgc ctccacgctc ttcacggtgc tcacgtacct
     1801 agtggacatg cggcgcttca gctacccgga acggcccatc attttcctgt ccggctgtta
     1861 cacagcggtg gcggtggcct acatcgctgg ctttctgttg gaggaccggg tggtgtgcaa
     1921 cgacaagttt gcagaggacg gggcgcgcac ggtggcgcag ggcactaaga aagaaggctg
     1981 cactatactc tttatgatgc tctacttctt cagcatggcc agctccatct ggtgggtgat
     2041 cctgtccctc acctggttcc tggcagccgg catgaagtgg ggccacgaag ccatcgaggc
     2101 caactcacag tatttccatt tagccgcctg ggctgtgcca gccatcaaaa ctataaccat
     2161 cttggcgttg ggccaggtgg atggcgacgt actgagcgga gtgtgttttg tggggctcaa
     2221 caacgtggac gcactgcgtg gctttgtgct ggcgcctctc ttcgtttatc tgttcattgg
     2281 cacttctttc ctgctggccg gtttcgtgtc actc
//
