LOCUS       sequence-310858-       11233 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tol2 vector for ubiquitous expression of QFGal4. Contains 
            cmlc2:mRFP-SV40pA transgenesis marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11233)
  AUTHORS   Burgess J, Burrows JT, Sadhak R, Chiang S, Weiss A, D'Amata C, 
            Molinaro AM, Zhu S, Long M, Hu C, Krause HM, Pearson BJ
  TITLE     An optimized QF-binary expression system for use in zebrafish.
  JOURNAL   Dev Biol. 2020 Jul 19. pii: S0012-1606(20)30202-5. doi: 
            10.1016/j.ydbio.2020.07.007.
  PUBMED    32697972
REFERENCE   2  (bases 1 to 11233)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Biol. 
            2020 Jul 19. pii: S0012-1606(20)30202-5. doi: 
            10.1016/j.ydbio.2020.07.007."
COMMENT     Sequence Label: pTol2CG2-ubi:QFGal4-SV40pA
FEATURES             Location/Qualifiers
     source          1..11233
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    3411..3435
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      3452..3461
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4115..4459
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     primer_bind     complement(4673..4689)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4716..4740)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4754..4861
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     4778..4794
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4828..4844)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4863..4883)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(4891..4907)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    4923..5057
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      6155..6164
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      6209..6218
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      6272..6281
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6278..6955
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             6278..6955
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(6408..6426)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6676..6695
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     6870..6893
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    join(7418..7605,7606..7617)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(7651..7667)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7704..7724)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7704..7722)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7743..7759)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7756..7778)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7767..7783
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7791..7797,7798..7815,7816..7821))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7836..7857
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7974..7991)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8145..8733)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8225..8244)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8904..9695,9696..9764))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9527..9546
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9765..9869)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10032..10053)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(10506..10517,10518..10680)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     11053..11069
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    11089..11109
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 caacagacgg caaaatgaat aaatgataac aaagcagtag gcttaaataa acctaatttt
       61 tataggctgt tctctacaac cctcaaacag tgattagttt tgtacttata aacttgccct
      121 ttcattcata tttcaagaaa attggttcag aagatctgga tattctagca gttgttcaag
      181 ctcatggagg gatcagtgac ctgattccac aatgactagg cctaatccag aaattagatg
      241 actgtcaaca taaaaaggca cagcactcac tagctgccct atatatttta ttatatttta
      301 catatattat tttatttatt tagctctgag tgctgtactt tctggttaaa gaaaactgct
      361 tacaacagct aacctgtact acctcaggct cagggaattt ggaacaggtt tgtctggttt
      421 gtttctttaa ccatgcatgc ttgttttcaa ctatggcaac acagtcacat gggacattac
      481 agaaatgatt tgtcgatgac atgcgacttt tctttaataa agcgcaaaga tcccaaaaag
      541 caaactttta acaaaaatca tataattata ttttcaatcc agctttgtag caactttgtg
      601 ctgctgttca ctcagcaaca gatagtcagt ataaggtcag tgtgtctcaa agcagtgcca
      661 tctgtttcac acattgcgtt ctatatataa gtgtgctggt tgacacgaca ctgtataagg
      721 cctaggctaa aacacaaaca atgtagaatg acactgtgtt ttttttgtaa acaaatgttg
      781 tttttggtta aacatctttg tgaaaacatc ctcctgtcat gtatttgcta tattcaaatg
      841 ttaaacccgt gcagaataga acatatacaa aaaaaaacaa cacaacacat ttttaaacat
      901 tattaaatat caagtattgc tggcagttct gtttctgttt tacagtaccc tttgccacag
      961 ttctccgctt ttcctggtcc agattccaca agtctgattc accaatagca aagcgaataa
     1021 acaaccaaag cagccaatca ctgcttgtag actgtcctgc gagaccggcc cattccagca
     1081 cattctggaa acttccttta tatgataatt ataaatacat ttaaattatt gatacaaaac
     1141 atgtaattcc tagaacataa ccatagcaat cattagtttt cagggtaatt atgtattttt
     1201 aggatttgac tgcggaaaga tctggtcatg tgacgtctca tgaacgtcac ggccctgggt
     1261 ttctataaat acagtaggac tctcgaccat cggcagattt ttcgaagaag aagatcagtt
     1321 tcaggagccg tactgttccg tttcaacgca aatattaacg gtaagagcga atttcctagt
     1381 ttgttttcat gccattcttt aaaaccatag cgtattactt taattatagt aaactttcgc
     1441 tttctttatt acaagagacg ttttgtgttg attctccgcg gacattttcg gtcagacaat
     1501 cagaaaatga ccgcggagga ccagtaactt gcattacacg taagttaaat cttcgtgtat
     1561 taaaatggtt aggttgttaa cgtcaaatag gttaccgtgt ttgcgtgtga tcaggttggt
     1621 tttgttagat ttttgtcagt atttttaatt tatttgtttt agtttattta ttttttttgc
     1681 tgaatcatag tttgtgaaca aagaacccgg atgttacata cagtacagcc gccatgttac
     1741 agagagttat aacttaatca ttttaaaaat aattttgcct tacttttagt ttgtcatgtt
     1801 gagaaatgag gaaatgttaa aatgaggaaa tatccaatta atttaatata tcaaaataat
     1861 ccatgattac aatgcactga actggagaaa attaagatgt tttctagtgt catgaaacaa
     1921 atgtaagaga tgtacattgt agatgtttta tgtcaagaat tggctagttg atgcagcata
     1981 ctggcgatac tcagttgtaa taacagtaac gttacatgtt aatagactac tgagtatgct
     2041 gttctgtcta tgtatgctct gtaagctacg agaaggactt ttttaaacag taaagggtgc
     2101 aatattttta caaattgaat taaataaagg ctgtctatta agtaatatgc ttgatatttt
     2161 tcttacttga tcggaaataa gaaaaaatat aaacgttgtt gctctaaaaa tcctagttca
     2221 gtttagccaa ccacaaatac ctttttgttc ctccaacagt ttttttttct tctctataat
     2281 atttggcagt ctatagtact ccaaatgttt ccccacagtc taactaattg gtacagccaa
     2341 aatcatgaca cttattgcaa taataatttt ggttcattgg cattgttgat agcctgtgcc
     2401 actaatatgg tcgattgatc atgcttcagg aagaaaacta tattgtttga tgtaagatta
     2461 ttaaatcttc acctgcttcc attacaaact attcccatct tattgaattc tggtatgtct
     2521 taaaggatta gttcacttcc caaatcaaaa tttacttagc cttttttcat ccatgatccc
     2581 tttttttcat cattaatgaa gaaattgttt ttgaaaaagt ttcaagattt ttttctctat
     2641 attgtggagc ttgttagttt aaaattccaa aatgcaatat gtggcttcaa atggttctaa
     2701 atgatcccag tcaaggaata acagtcttat ctaatgaaac cattagacct ttaaaaataa
     2761 aaaaaataaa agtatttatt tttaaatgac tgagtgatta agttgaattt cagcgtttcc
     2821 ttactgtgta gaagtccttc cttactggcc ccaccctttg gttctctgcc aatctgctac
     2881 ctaatgtaat gttgtggaac attattattc tttatttctt aattttttat tttttatttt
     2941 aaaacaatgt aaactgcaca gatgtgcagt ttgtttaaaa tggccaatgc tttggaaatg
     3001 catgacataa ttagatttca tgatgcacaa agccaaatct cagagcttgt gcaaaatgag
     3061 ctatcatttc actaggtaag accctaaatt ttcatatagg atcatttgga caattttgct
     3121 gcaggtaaaa tgcattctat agtccactgt cagccattgt tttggatagt atttattttt
     3181 ctctacaagt atagtcaata gttttctatt attttaaagg tttgtaacat ttaagggtga
     3241 ccaaatgcaa agtaaaattt cattttcggg tgaactatct cgtttaacat gggagaagtg
     3301 caaaacatac attattggct agaacattgt agtatttttt aaatggaaat gtgtgattgc
     3361 taatcttact ttgaatttgt ttacagggat cctttaaggg cgaattcgac ccaagtttgt
     3421 acaaaaaagc aggctcgaat tggagctcgc cgccaccatg gcacccaaga agaagaggaa
     3481 gatgccgcct aaacgcaaga cactcaatgc cgctgccgaa gccaatgccc acgctgatgg
     3541 ccatgctgat ggcaatgctg atggtcatgt cgctaacact gcagcaagca gcaacaacgc
     3601 ccgttttgcg gacttgacca acattgacac acccggcctc ggccctacca cgacgacgtt
     3661 acttgtcgag cccgctcgtt cgaaacgcca gagagtctcg agggcctgtg atcagtgtcg
     3721 agctgcacgt gaaaagtgtg atggaatcca gccggcttgc ttcccctgtg tgtcgcaggg
     3781 ccggtcgtgt acctaccagg ccagtcccaa gaagcgagga gtccagacgg gctacatccg
     3841 cactctcgaa ctggctctgg cttggatgtt cgagaacgtt gcccgcagcg aggacgccct
     3901 ccacaatctt ttggtccgtg atgctggcca gggcagcgct ctcctggtcg gcaaagactc
     3961 gcctgctgca gaacgcctgc atgcaagatg ggcgacgagt cgagtcaaca aaagcatcac
     4021 ccgtcttctc tcaggtgcta gcactagtag cgccggtcaa cagcaacagc tgcaatcatt
     4081 agtgccactg accccgtctg ctttgtttgg tggcgccaat tttaatcaaa gtgggaatat
     4141 tgctgatagc tcattgtcct tcactttcac taacagtagc aacggtccga acctcataac
     4201 aactcaaaca aattctcaag cgctttcaca accaattgcc tcctctaacg ttcatgataa
     4261 cttcatgaat aatgaaatca cggctagtaa aattgatgat ggtaataatt caaaaccact
     4321 gtcacctggt tggacggacc aaactgcgta taacgcgttt ggaatcacta cagggatgtt
     4381 taataccact acaatggatg atgtatataa ctatctattc gatgatgaag ataccccacc
     4441 aaacccaaaa aaagagtaac ccgggagcgg cccctctcga gcctctagaa ctatagtgag
     4501 tcgtattacg tagatccaga catgataaga tacattgatg agtttggaca aaccacaact
     4561 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
     4621 accattataa gctgcaataa acaagagatc tgatctagag gatcaagctt atcgataccg
     4681 tcgacctcga gggggggccc ggtacccagc ttttgaccca gctttcttgt acaaagtggg
     4741 aagatctacc ggtgagctcc accgcggtgg cggccgctct agaactagtg gatcccccgg
     4801 gctgcaggaa ttcgatatca agcttatcga taccgtcgac ctcgaggggg ggcccggtac
     4861 cgcaacttta ttatacatag ttgataattc actggccgtc gttttacggt accatcgatg
     4921 atgatccaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
     4981 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
     5041 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
     5101 aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg
     5161 atcctctaga tcagatcttg accaaagctt aaatcagttg tgttaaataa gagacattca
     5221 aaataaatgt aaatgagctc tccaaatcag cagacttaac attctttaaa atgattgatt
     5281 caatagtgat aaaaatcagg catagccagt tgtaacttta gataaattac agaaaatgtc
     5341 aaatacagag aaccgattct tttttatgat acatccaagc acacatttaa cacaatccag
     5401 gcaaaccccg aatttcacag tcacaagcac tgtttgtaca agagctttgc ctaaggacac
     5461 acagtctcta taagtccagg tcgttggttt cactcttatt ttaaacatgt gacatttttc
     5521 ctgccatcct gtcttaggct gctgtttgct tcattccatg tcacattaat ttcctcagta
     5581 gcacctttta cacacacagc caatcttttc cagaaaattc aattgctttg aagagataat
     5641 gtgtgaacaa atccatttag aaaaggaaaa ttaagaattt gtaaaatcat ctgtaaattg
     5701 ttggcattct tctgtatatg aacatcacat catttacagg taaaggtctg gtcattaatt
     5761 atatgacaat ttactggtat tattttgtga aaggggctat tttcaatgcg ttcatccatc
     5821 cttttcatcc ctcaaatctc tcattcacgt ccccctcccc atctgcacac tttatctcat
     5881 tttccaccct gctggaatct gagcacttgt gcagttatca gggctcctgt atttaggagg
     5941 ctctgggtgt ccatgtaggg gacgaacaga aacactgcag acctttatag aagaacaaat
     6001 gataagagtc ctcgtacata aagactccat tagtaacgtc agtgacccag gagcccagac
     6061 caacagcaaa gcagacagtg aaatcactag tgaattcgcg gccgcctgca ggtcgacggt
     6121 atcgattcga attcaaggcc tctcgagcct ctaagccacc atgggctgca tcaagagcaa
     6181 gcgcaaggac aacctgaacg acgacgaggc cgccatgggc tgcatcaaga gcaagcgcaa
     6241 ggacaacctg aacgacgacg aggcaccggt cgccaccatg gcctcctccg aggacgtcat
     6301 caaggagttc atgcgcttca aggtgcgcat ggagggctcc gtgaacggcc acgagttcga
     6361 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
     6421 gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tccagtacgg
     6481 ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc
     6541 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac
     6601 ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa
     6661 cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctccaccga
     6721 gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagatgaggc tgaagctgaa
     6781 ggacggcggc cactacgacg ccgaggtcaa gaccacctac atggccaaga agcccgtgca
     6841 gctgcccggc gcctacaaga ccgacatcaa gctggacatc acctcccaca acgaggacta
     6901 caccatcgtg gaacagtacg agcgcgccga gggccgccac tccaccggcg cctaatacgt
     6961 agatccagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
     7021 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     7081 ctgcaataaa caagagatct gcgaagatac ggccacgggt gctcttgatc ctgtggctga
     7141 ttttggactg tgctgctcgc agctgctgat gaatcacata cttcctccat tttcttccac
     7201 tgattgactg ttataatttc cctaatttcc aggtcaaggt gctgtgcatt gtggtaatag
     7261 atgtgacatg acgtcacttc caaaggacca atgaacatgt ctgaccaatt tcatataatg
     7321 tgaaaacgat tttcataggc agaataaata acatttaaat taaactgggc atcagcgcaa
     7381 ttcaattggt ttggtaatag caagggaaaa tagaatgaag tgatctccaa aaaataagta
     7441 ctttttgact gtaaataaaa ttgtaaggag taaaaagtac ttttttttct aaaaaaatgt
     7501 aattaagtaa aagtaaaagt attgattttt aattgtactc aagtaaagta aaaatcccca
     7561 aaaataatac ttaagtacag taatcaagta aaattactca agtactttac acctctggtt
     7621 cttgaccccc taccttcagc aagcccagca gatccactag ttctagagcg gccgccaccg
     7681 cggtggagct ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca
     7741 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     7801 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     7861 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     7921 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     7981 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     8041 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     8101 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     8161 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     8221 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     8281 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     8341 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     8401 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     8461 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     8521 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     8581 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     8641 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     8701 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     8761 tctgacgctc agtggaacga aaactcacgt taaggcattt tggtcatgag attatcaaaa
     8821 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     8881 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     8941 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     9001 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     9061 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     9121 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     9181 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     9241 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     9301 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     9361 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     9421 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     9481 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     9541 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     9601 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     9661 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     9721 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     9781 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     9841 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctaaattg
     9901 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
     9961 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
    10021 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
    10081 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
    10141 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
    10201 ttagagcttg acggggaaag gctggctaag aactcatcag cctccccggt ccatctaccc
    10261 acgtaccaat gcaccaattg gccacaatga cggctactac atggtgccat tccttcctct
    10321 ttataggaat ggagactacc tcctgtccaa caatgctctt ggatacgagt acgcctacct
    10381 gttggaccca ggtcattgca caacaccaga aatgccctct gatctgcaaa agacgtgaat
    10441 atctgttcag acacccatat ccactctgtt ccacacaggt cagaggtttg tccaggagtt
    10501 cttgacagag gtgtaaaaag tactcaaaaa ttttactcaa gtgaaagtac aagtacttag
    10561 ggaaaatttt actcaattaa aagtaaaagt atctggctag aatcttactt gagtaaaagt
    10621 aaaaaagtac tccattaaaa ttgtacttga gtattaagga agtaaaagta aaagcaagaa
    10681 agaaaactag agattcttgt ttaagctttt aatctcaaaa aacattaaat gaaatgcata
    10741 caaggtttta tcctgcttta gaactgtttg tatttaatta tcaaactata agacagacaa
    10801 tctaatgcca gtacacgcta ctcaaagttg taaaacctca gatttaactt cagtagaagc
    10861 tgattctcaa aattgttagt gtcaagccta gctcttttgg ggctgaaaag caatcctgca
    10921 gtgctgaaaa gcctctcaca ggcagccgat gcgggaagag gtgtattagt cttgatagag
    10981 aggctgcaaa tagcaggaaa cgtgagcaga gactccctgg tgtctgaaac acaggccaga
    11041 tgggccctcg agcaggaaac agctatgacc atgattacgc caagctatca actttgtata
    11101 gaaaagttgg ctccgaattc gcccttaaaa ctcgagacca gcaaagttct agaatttgtc
    11161 gaaacattta tgttatatat ttcctgaaaa aaattctgag taagttctta agtgttattg
    11221 ccagcaacat aaa
//
