LOCUS       sequence-310859-        7863 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tol2 vector containing QUAS5x reporter element upstream of GFP-NLS. 
            Includes cmlc2:mRFP-SV40pA transgenesis marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7863)
  AUTHORS   Burgess J, Burrows JT, Sadhak R, Chiang S, Weiss A, D'Amata C, 
            Molinaro AM, Zhu S, Long M, Hu C, Krause HM, Pearson BJ
  TITLE     An optimized QF-binary expression system for use in zebrafish.
  JOURNAL   Dev Biol. 2020 Jul 19. pii: S0012-1606(20)30202-5. doi: 
            10.1016/j.ydbio.2020.07.007.
  PUBMED    32697972
REFERENCE   2  (bases 1 to 7863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Biol. 
            2020 Jul 19. pii: S0012-1606(20)30202-5. doi: 
            10.1016/j.ydbio.2020.07.007."
COMMENT     Sequence Label: pTol2CG2-QUAS5x:GFPNLS-SV40pA
FEATURES             Location/Qualifiers
     source          1..7863
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      162..171
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(168..170,171..173,174..884)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(168..170,171..173,174..884)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(213..234)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(474..493)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     821..842
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1184..1200)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1227..1251)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    1253..1360
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     1270..1286
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1320..1336)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1374..1394)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(1402..1418)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    1434..1568
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      2666..2675
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2720..2729
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2783..2792
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2789..3466
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             2789..3466
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(2919..2937)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3187..3206
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3381..3404
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    join(3929..4116,4117..4128)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(4162..4178)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4215..4235)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4215..4233)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4254..4270)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4267..4289)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4278..4294
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4302..4308,4309..4326,4327..4332))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4347..4368
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4485..4502)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4656..5244)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4736..4755)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5415..6206,6207..6275))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6038..6057
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6276..6380)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6543..6564)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(7017..7028,7029..7191)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     7564..7580
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7600..7620
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    7630..7737
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     7647..7663
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7697..7713)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7743..7767
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 taaacaatta tcctcaagga taaacaatta tcctcgcaag ggtcgactct agagggtata
       61 taatggatcc catcgcgtct cagcctcact ttgagctcct ccacacgaat tccctcgacc
      121 tcgaagacgc gtctgtttgg gatccaccgg tcactagtgc cgccaccatg gtgagcaagg
      181 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
      241 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
      301 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
      361 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
      421 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
      481 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
      541 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
      601 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
      661 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
      721 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
      781 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
      841 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagagcagg cacagaaggc
      901 atcgccagcg ctctaggagc cgcaatcgca gccgaagtcg cagcagtgaa cgaaaacgcc
      961 gtcaacggag ccgaagtcgc agcagtgaac gaagacgcta cttgtacaag taatctagaa
     1021 ctatagtgag tcgtattacg tagatccaga catgataaga tacattgatg agtttggaca
     1081 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     1141 tttatttgta accattataa gctgcaataa acaagaagct tatcgatacc gtcgacctcg
     1201 agggggggcc cggtacccag cttttgaccc agctttcttg tacaaagtgg gaggtaccgg
     1261 gccccccctc gaggtcgacg gtatcgataa gcttgatatc gaattcctgc agcccggggg
     1321 atccactagt tctagagcgg ccgccaccgc ggtggagctc accggtagat ctgcaacttt
     1381 attatacata gttgataatt cactggccgt cgttttacgg taccatcgat gatgatccag
     1441 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
     1501 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
     1561 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
     1621 aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat gatcctctag
     1681 atcagatctt gaccaaagct taaatcagtt gtgttaaata agagacattc aaaataaatg
     1741 taaatgagct ctccaaatca gcagacttaa cattctttaa aatgattgat tcaatagtga
     1801 taaaaatcag gcatagccag ttgtaacttt agataaatta cagaaaatgt caaatacaga
     1861 gaaccgattc ttttttatga tacatccaag cacacattta acacaatcca ggcaaacccc
     1921 gaatttcaca gtcacaagca ctgtttgtac aagagctttg cctaaggaca cacagtctct
     1981 ataagtccag gtcgttggtt tcactcttat tttaaacatg tgacattttt cctgccatcc
     2041 tgtcttaggc tgctgtttgc ttcattccat gtcacattaa tttcctcagt agcacctttt
     2101 acacacacag ccaatctttt ccagaaaatt caattgcttt gaagagataa tgtgtgaaca
     2161 aatccattta gaaaaggaaa attaagaatt tgtaaaatca tctgtaaatt gttggcattc
     2221 ttctgtatat gaacatcaca tcatttacag gtaaaggtct ggtcattaat tatatgacaa
     2281 tttactggta ttattttgtg aaaggggcta ttttcaatgc gttcatccat ccttttcatc
     2341 cctcaaatct ctcattcacg tccccctccc catctgcaca ctttatctca ttttccaccc
     2401 tgctggaatc tgagcacttg tgcagttatc agggctcctg tatttaggag gctctgggtg
     2461 tccatgtagg ggacgaacag aaacactgca gacctttata gaagaacaaa tgataagagt
     2521 cctcgtacat aaagactcca ttagtaacgt cagtgaccca ggagcccaga ccaacagcaa
     2581 agcagacagt gaaatcacta gtgaattcgc ggccgcctgc aggtcgacgg tatcgattcg
     2641 aattcaaggc ctctcgagcc tctaagccac catgggctgc atcaagagca agcgcaagga
     2701 caacctgaac gacgacgagg ccgccatggg ctgcatcaag agcaagcgca aggacaacct
     2761 gaacgacgac gaggcaccgg tcgccaccat ggcctcctcc gaggacgtca tcaaggagtt
     2821 catgcgcttc aaggtgcgca tggagggctc cgtgaacggc cacgagttcg agatcgaggg
     2881 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
     2941 cggccccctg cccttcgcct gggacatcct gtcccctcag ttccagtacg gctccaaggc
     3001 ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt
     3061 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc
     3121 ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc
     3181 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctccaccg agcggatgta
     3241 ccccgaggac ggcgccctga agggcgagat caagatgagg ctgaagctga aggacggcgg
     3301 ccactacgac gccgaggtca agaccaccta catggccaag aagcccgtgc agctgcccgg
     3361 cgcctacaag accgacatca agctggacat cacctcccac aacgaggact acaccatcgt
     3421 ggaacagtac gagcgcgccg agggccgcca ctccaccggc gcctaatacg tagatccaga
     3481 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
     3541 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
     3601 acaagagatc tgcgaagata cggccacggg tgctcttgat cctgtggctg attttggact
     3661 gtgctgctcg cagctgctga tgaatcacat acttcctcca ttttcttcca ctgattgact
     3721 gttataattt ccctaatttc caggtcaagg tgctgtgcat tgtggtaata gatgtgacat
     3781 gacgtcactt ccaaaggacc aatgaacatg tctgaccaat ttcatataat gtgaaaacga
     3841 ttttcatagg cagaataaat aacatttaaa ttaaactggg catcagcgca attcaattgg
     3901 tttggtaata gcaagggaaa atagaatgaa gtgatctcca aaaaataagt actttttgac
     3961 tgtaaataaa attgtaagga gtaaaaagta cttttttttc taaaaaaatg taattaagta
     4021 aaagtaaaag tattgatttt taattgtact caagtaaagt aaaaatcccc aaaaataata
     4081 cttaagtaca gtaatcaagt aaaattactc aagtacttta cacctctggt tcttgacccc
     4141 ctaccttcag caagcccagc agatccacta gttctagagc ggccgccacc gcggtggagc
     4201 tccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag
     4261 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     4321 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     4381 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     4441 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     4501 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     4561 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     4621 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     4681 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     4741 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     4801 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     4861 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     4921 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     4981 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     5041 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     5101 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     5161 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     5221 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     5281 cagtggaacg aaaactcacg ttaaggcatt ttggtcatga gattatcaaa aaggatcttc
     5341 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     5401 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     5461 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     5521 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     5581 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     5641 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     5701 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     5761 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     5821 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     5881 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     5941 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     6001 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     6061 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     6121 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     6181 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     6241 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     6301 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     6361 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta
     6421 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
     6481 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
     6541 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
     6601 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
     6661 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
     6721 gacggggaaa ggctggctaa gaactcatca gcctccccgg tccatctacc cacgtaccaa
     6781 tgcaccaatt ggccacaatg acggctacta catggtgcca ttccttcctc tttataggaa
     6841 tggagactac ctcctgtcca acaatgctct tggatacgag tacgcctacc tgttggaccc
     6901 aggtcattgc acaacaccag aaatgccctc tgatctgcaa aagacgtgaa tatctgttca
     6961 gacacccata tccactctgt tccacacagg tcagaggttt gtccaggagt tcttgacaga
     7021 ggtgtaaaaa gtactcaaaa attttactca agtgaaagta caagtactta gggaaaattt
     7081 tactcaatta aaagtaaaag tatctggcta gaatcttact tgagtaaaag taaaaaagta
     7141 ctccattaaa attgtacttg agtattaagg aagtaaaagt aaaagcaaga aagaaaacta
     7201 gagattcttg tttaagcttt taatctcaaa aaacattaaa tgaaatgcat acaaggtttt
     7261 atcctgcttt agaactgttt gtatttaatt atcaaactat aagacagaca atctaatgcc
     7321 agtacacgct actcaaagtt gtaaaacctc agatttaact tcagtagaag ctgattctca
     7381 aaattgttag tgtcaagcct agctcttttg gggctgaaaa gcaatcctgc agtgctgaaa
     7441 agcctctcac aggcagccga tgcgggaaga ggtgtattag tcttgataga gaggctgcaa
     7501 atagcaggaa acgtgagcag agactccctg gtgtctgaaa cacaggccag atgggccctc
     7561 gagcaggaaa cagctatgac catgattacg ccaagctatc aactttgtat agaaaagttg
     7621 caaaagctgg gtaccgggcc ccccctcgag gtcgacggta tcgataagct tgatatcgaa
     7681 ttcctgcagc ccgggggatc cactagttct agagcggccg ccaccgcggt ggagctccaa
     7741 ttgcaagttt gtacaaaaaa gcaggctcga attggagctc caccgcggtg gcggccgcaa
     7801 ggataaacaa ttatcctcaa ggataaacaa ttatcctcaa ggataaacaa ttatcctcaa
     7861 gga
//
