LOCUS       sequence-310861-       10451 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tol2 vector containing Cre-inducible QFGal4-switch.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10451)
  AUTHORS   Burgess J, Burrows JT, Sadhak R, Chiang S, Weiss A, D'Amata C, 
            Molinaro AM, Zhu S, Long M, Hu C, Krause HM, Pearson BJ
  TITLE     An optimized QF-binary expression system for use in zebrafish.
  JOURNAL   Dev Biol. 2020 Jul 19. pii: S0012-1606(20)30202-5. doi: 
            10.1016/j.ydbio.2020.07.007.
  PUBMED    32697972
REFERENCE   2  (bases 1 to 10451)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Biol. 
            2020 Jul 19. pii: S0012-1606(20)30202-5. doi: 
            10.1016/j.ydbio.2020.07.007."
COMMENT     Sequence Label: pTol2-ubi:loxP-mScarletNLS-SV40pA-loxp-QFGal4-...
FEATURES             Location/Qualifiers
     source          1..10451
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(611..635)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(1069..1231,1232..1243)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     1593..1614
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1777..1881
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1882..1950,1951..2742)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2100..2119)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2913..3501
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3402..3421
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3655..3672
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3789..3810
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3825..3830,3831..3848,3849..3855)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3863..3879
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3868..3890
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3887..3903
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3887..3903
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3922..3942
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3924..3942
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     3979..3995
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3979..3995
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     misc_feature    join(4029..4040,4041..4228)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     polyA_signal    4655..4789
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(4832..4888)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     polyA_signal    5172..5306
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(5350..5694)
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     regulatory      6348..6357
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    6375..6408
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    6475..6609
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    6649..6669
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      7499..7508
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    7527..7560
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 agtcgcatgt catcgacaaa tcatttctgt aatgtcccat gtgactgtgt tgccatagtt
       61 gaaaacaagc atgcatggtt aaagaaacaa accagacaaa cctgttccaa attccctgag
      121 cctgaggtag tacaggttag ctgttgtaag cagttttctt taaccagaaa gtacagcact
      181 cagagctaaa taaataaaat aatatatgta aaatataata aaatatatag ggcagctagt
      241 gagtgctgtg cctttttatg ttgacagtca tctaatttct ggattaggcc tagtcattgt
      301 ggaatcaggt cactgatccc tccatgagct tgaacaactg ctagaatatc cagatcttct
      361 gaaccaattt tcttgaaata tgaatgaaag ggcaagttta taagtacaaa actaatcact
      421 gtttgagggt tgtagagaac agcctataaa aattaggttt atttaagcct actgctttgt
      481 tatcatttat tcattttgcc gtctgttgtt tatgttgctg gcaataacac ttaagaactt
      541 actcagaatt tttttcagga aatatataac ataaatgttt cgacaaattc tagaactttg
      601 ctgggtcgac agcctgcttt tttgtacaaa cttgtctcga gatgggccct tgatatcgat
      661 atcattaccc tgttatccct atacgaaatt aagcgtacga atggcccatc tggcctgtgt
      721 ttcagacacc agggagtctc tgctcacgtt tcctgctatt tgcagcctct ctatcaagac
      781 taatacacct cttcccgcat cggctgcctg tgagaggctt ttcagcactg caggattgct
      841 tttcagcccc aaaagagcta ggcttgacac taacaatttt gagaatcagc ttctactgaa
      901 gttaaatctg aggttttaca actttgagta gcgtgtactg gcattagatt gtctgtctta
      961 tagtttgata attaaataca aacagttcta aagcaggata aaaccttgta tgcatttcat
     1021 ttaatgtttt ttgagattaa aagcttaaac aagaatctct agttttcttt cttgctttta
     1081 cttttacttc cttaatactc aagtacaatt ttaatggagt acttttttac ttttactcaa
     1141 gtaagattct agccagatac ttttactttt aattgagtaa aattttccct aagtacttgt
     1201 actttcactt gagtaaaatt tttgagtact ttttacacct ctgtcaagaa ctcctggaca
     1261 aacctctgac ctgtgtggaa cagagtgcat atgggtgtct gaacagatta ttcacgtctt
     1321 ttgcagatca gagggcattt ctggtgttgt gcaatgacct gggtccaaca ggtaggcgta
     1381 ctcgtatcca agagcattgt tggacaggag gtagtctcca ttcctataaa gaggaaggaa
     1441 tggcaccatg tagtagccgt cattgtggcc aattggtgca ttggtacgtg ggtagatgga
     1501 ccggggaggc tgatgagttc ttagccagcg tgggccatcg ccctgataga cggtttttcg
     1561 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     1621 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     1681 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     1741 gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     1801 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     1861 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     1921 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     1981 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     2041 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     2101 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     2161 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     2221 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     2281 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     2341 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     2401 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     2461 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     2521 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     2581 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     2641 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     2701 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     2761 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     2821 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     2881 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     2941 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     3001 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     3061 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     3121 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     3181 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     3241 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     3301 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     3361 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     3421 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     3481 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     3541 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     3601 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     3661 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     3721 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     3781 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     3841 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     3901 gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctc
     3961 caccgcggtg gcggccgctc tagaactagt ggatctgctg ggcttgctga aggtaggggg
     4021 tcaagaacca gaggtgtaaa gtacttgagt aattttactt gattactgta cttaagtatt
     4081 atttttgggg atttttactt tacttgagta caattaaaaa tcaatacttt tacttttact
     4141 taattacatt tttttagaaa aaaaagtact ttttactcct tacaatttta tttacagtca
     4201 aaaagtactt attttttgga gatcacttca ttctattttc ccttgctatt accaaaccaa
     4261 ttgaattgcg ctgatgccca gtttaattta aatgttattt attctgccta tgaaaatcgt
     4321 tttcacatta tatgaaattg gtcagacatg ttcattggtc ctttggaagt gacgtcatgt
     4381 cacatctatt accacaatgc acagcacctt gacctggaaa ttagggaaat tataacagtc
     4441 aatcagtgga agaaaatgga ggaagtatgt gattcatcag cagctgcgag cagcacagtc
     4501 caaaatcagc cacaggatca agagcacccg tggccgtatc ttcgcagatc acgcgtgtgg
     4561 ccggccaagg taccgtacct ggttacccgg ggaaaaaaac ctcccacacc tccccctgaa
     4621 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     4681 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     4741 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatct acgtaatacg
     4801 actcactata gttctagagg ctcgcgatcg caggtccagg gttctcctcc acgtctccag
     4861 cctgcttcag caggctgaag ttagtagctc cgcttccgtt taaactactt cgaggtcgag
     4921 ggaattcgtg tggaggagct caaagtgagg ctgagacgcg atgggatcca ttatataccc
     4981 tctagagtcg acccttgcga ggataattgt ttatccttga ggataattgt ttatccttga
     5041 ggataattgt ttatccttga ggataattgt ttatccttga ggataattgt ttatccttga
     5101 ttaattaaga aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     5161 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     5221 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     5281 tcaatgtatc ttatcatgtc tggatctacg taatacgact cactatagtt ctagaggctc
     5341 ggcgcgcctt tactcttttt ttgggtttgg tggggtatct tcatcatcga atagatagtt
     5401 atatacatca tccattgtag tggtattaaa catccctgta gtgattccaa acgcgttata
     5461 cgcagtttgg tccgtccaac caggtgacag tggttttgaa ttattaccat catcaatttt
     5521 actagccgtg atttcattat tcatgaagtt atcatgaacg ttagaggagg caattggttg
     5581 tgaaagcgct tgagaatttg tttgagttgt tatgaggttc ggaccgttgc tactgttagt
     5641 gaaagtgaag gacaatgagc tatcagcaat attcccactt tgattaaaat tggcgccacc
     5701 aaacaaagca gacggggtca gtggcactaa tgattgcagc tgttgctgtt gaccggcgct
     5761 actagtgcta gcacctgaga gaagacgggt gatgcttttg ttgactcgac tcgtcgccca
     5821 tcttgcatgc aggcgttctg cagcaggcga gtctttgccg accaggagag cgctgccctg
     5881 gccagcatca cggaccaaaa gattgtggag ggcgtcctcg ctgcgggcaa cgttctcgaa
     5941 catccaagcc agagccagtt cgagagtgcg gatgtagccc gtctggactc ctcgcttctt
     6001 gggactggcc tggtaggtac acgaccggcc ctgcgacaca caggggaagc aagccggctg
     6061 gattccatca cacttttcac gtgcagctcg acactgatca caggccctcg agactctctg
     6121 gcgtttcgaa cgagcgggct cgacaagtaa cgtcgtcgtg gtagggccga ggccgggtgt
     6181 gtcaatgttg gtcaagtccg caaaacgggc gttgttgctg cttgctgcag tgttagcgac
     6241 atgaccatca gcattgccat cagcatggcc atcagcgtgg gcattggctt cggcagcggc
     6301 attgagtgtc ttgcgtttag gcggcatctt cctcttcttc ttgggtgcca tggtggcggc
     6361 gacctgcagg cggtataact tcgtataatg tatgctatac gaagttatct cgaaaaaaac
     6421 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     6481 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     6541 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     6601 gtctggatct acgtaatacg actcactata gttctagagg ctcgagtgca actatgtata
     6661 ataaagttgc accggtttta cttgtacaag tagcgtcttc gttcactgct gcgacttcgg
     6721 ctccgttgac ggcgttttcg ttcactgctg cgacttcggc tgcgattgcg gctcctagag
     6781 cgctggcgat gccttctgtg cctgctcttg tacagctcgt ccattccgcc tgtgctgtgg
     6841 cgtccctcgc ttctctcgta ctgttccacc acggtgtagt cctcgttgtg gctggtgatg
     6901 tccagctttc tgtccacgtt gtaggcgcca ggcatctgca caggtttctt ggccttgtag
     6961 gtggtcttga agtcggccag gtatcttccg ccgtctttca gtctcagggc catcttgatg
     7021 tcgcccttca gcacgccatc ttcagggtac agtctctcgg tgctggcttc ccatcccatg
     7081 gtctttttct gcatcacagg tccgtcagga gggaagtttg ttccgcgcag cttcactttg
     7141 tagatcaggg ttccatcttc caggctggta tcctgtgtca ctgtcacggc tccgccgtcc
     7201 tcgaagttca tcactctctc ccacttgaat ccctcaggga agctctgctt gtagtagtca
     7261 gggatgtcag cagggtgctt tgtgaaggcg cggcttccgt acataaactg tggagacagg
     7321 atgtcccagc tgaaaggcag aggtccgcct ttggtcactt tcagcttggc ggtctgtgtt
     7381 ccctcgtaag gtctgccctc gccttcgcct tcgatctcga actcgtgtcc gttcatgctg
     7441 ccttccatgt gcaccttgaa gcgcatgaat tctttgatca cggcctcgcc cttgctcacc
     7501 atggtggcgg cgaaccggta gctcgtataa cttcgtataa tgtatgctat acgaagttat
     7561 cctaggttag atcctgtaaa caaattcaaa gtaagattag caatcacaca tttccattta
     7621 aaaaatacta caatgttcta gccaataatg tatgttttgc acttctccca tgttaaacga
     7681 gatagttcac ccgaaaatga aattttactt tgcatttggt cacccttaaa tgttacaaac
     7741 ctttaaaata atagaaaact attgactata cttgtagaga aaaataaata ctatccaaaa
     7801 caatggctga cagtggacta tagaatgcat tttacctgca gcaaaattgt ccaaatgatc
     7861 ctatatgaaa atttagggtc ttacctagtg aaatgatagc tcattttgca caagctctga
     7921 gatttggctt tgtgcatcat gaaatctaat tatgtcatgc atttccaaag cattggccat
     7981 tttaaacaaa ctgcacatct gtgcagttta cattgtttta aaataaaaaa taaaaaatta
     8041 agaaataaag aataataatg ttccacaaca ttacattagg tagcagattg gcagagaacc
     8101 aaagggtggg gccagtaagg aaggacttct acacagtaag gaaacgctga aattcaactt
     8161 aatcactcag tcatttaaaa ataaatactt ttattttttt tatttttaaa ggtctaatgg
     8221 tttcattaga taagactgtt attccttgac tgggatcatt tagaaccatt tgaagccaca
     8281 tattgcattt tggaatttta aactaacaag ctccacaata tagagaaaaa aatcttgaaa
     8341 ctttttcaaa aacaatttct tcattaatga tgaaaaaaag ggatcatgga tgaaaaaagg
     8401 ctaagtaaat tttgatttgg gaagtgaact aatcctttaa gacataccag aattcaataa
     8461 gatgggaata gtttgtaatg gaagcaggtg aagatttaat aatcttacat caaacaatat
     8521 agttttcttc ctgaagcatg atcaatcgac catattagtg gcacaggcta tcaacaatgc
     8581 caatgaacca aaattattat tgcaataagt gtcatgattt tggctgtacc aattagttag
     8641 actgtgggga aacatttgga gtactataga ctgccaaata ttatagagaa gaaaaaaaaa
     8701 ctgttggagg aacaaaaagg tatttgtggt tggctaaact gaactaggat ttttagagca
     8761 acaacgttta tattttttct tatttccgat caagtaagaa aaatatcaag catattactt
     8821 aatagacagc ctttatttaa ttcaatttgt aaaaatattg caccctttac tgtttaaaaa
     8881 agtccttctc gtagcttaca gagcatacat agacagaaca gcatactcag tagtctatta
     8941 acatgtaacg ttactgttat tacaactgag tatcgccagt atgctgcatc aactagccaa
     9001 ttcttgacat aaaacatcta caatgtacat ctcttacatt tgtttcatga cactagaaaa
     9061 catcttaatt ttctccagtt cagtgcattg taatcatgga ttattttgat atattaaatt
     9121 aattggatat ttcctcattt taacatttcc tcatttctca acatgacaaa ctaaaagtaa
     9181 ggcaaaatta tttttaaaat gattaagtta taactctctg taacatggcg gctgtactgt
     9241 atgtaacatc cgggttcttt gttcacaaac tatgattcag caaaaaaaat aaataaacta
     9301 aaacaaataa attaaaaata ctgacaaaaa tctaacaaaa ccaacctgat cacacgcaaa
     9361 cacggtaacc tatttgacgt taacaaccta accattttaa tacacgaaga tttaacttac
     9421 gtgtaatgca agttactggt cctccgcggt cattttctga ttgtctgacc gaaaatgtcc
     9481 gcggagaatc aacacaaaac gtctcttgta ataaagaaag cgaaagttta ctataattaa
     9541 agtaatacgc tatggtttta aagaatggca tgaaaacaaa ctaggaaatt cgctcttacc
     9601 gttaatattt gcgttgaaac ggaacagtac ggctcctgaa actgatcttc ttcttcgaaa
     9661 aatctgccga tggtcgagag tcctactgta tttatagaaa cccagggccg tgacgttcat
     9721 gagacgtcac atgaccagat ctttccgcag tcaaatccta aaaatacata attaccctga
     9781 aaactaatga ttgctatggt tatgttctag gaattacatg ttttgtatca ataatttaaa
     9841 tgtatttata attatcatat aaaggaagtt tccagaatgt gctggaatgg gccggtctcg
     9901 caggacagtc tacaagcagt gattggctgc tttggttgtt tattcgcttt gctattggtg
     9961 aatcagactt gtggaatctg gaccaggaaa agcggagaac tgtggcaaag ggtactgtaa
    10021 aacagaaaca gaactgccag caatacttga tatttaataa tgtttaaaaa tgtgttgtgt
    10081 tgtttttttt tgtatatgtt ctattctgca cgggtttaac atttgaatat agcaaataca
    10141 tgacaggagg atgttttcac aaagatgttt aaccaaaaac aacatttgtt tacaaaaaaa
    10201 acacagtgtc attctacatt gtttgtgttt tagcctaggc cttatacagt gtcgtgtcaa
    10261 ccagcacact tatatataga acgcaatgtg tgaaacagat ggcactgctt tgagacacac
    10321 tgaccttata ctgactatct gttgctgagt gaacagcagc acaaagttgc tacaaagctg
    10381 gattgaaaat ataattatat gatttttgtt aaaagtttgc tttttgggat ctttgcgctt
    10441 tattaaagaa a
//
