LOCUS       sequence-310874-        7864 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tol2 vector containing QUASM1 reporter element upstream of GFP-NLS. 
            Includes cmlc2:mRFP-SV40pA transgenesis marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7864)
  AUTHORS   Burgess J, Burrows JT, Sadhak R, Chiang S, Weiss A, D'Amata C, 
            Molinaro AM, Zhu S, Long M, Hu C, Krause HM, Pearson BJ
  TITLE     An optimized QF-binary expression system for use in zebrafish.
  JOURNAL   Dev Biol. 2020 Jul 19. pii: S0012-1606(20)30202-5. doi: 
            10.1016/j.ydbio.2020.07.007.
  PUBMED    32697972
REFERENCE   2  (bases 1 to 7864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Biol. 
            2020 Jul 19. pii: S0012-1606(20)30202-5. doi: 
            10.1016/j.ydbio.2020.07.007."
COMMENT     Sequence Label: pTol2CG2-QUASM1:GFPNLS-SV40pA
FEATURES             Location/Qualifiers
     source          1..7864
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(618..629,630..792)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     1165..1181
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1201..1221
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    1231..1338
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     1248..1264
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1298..1314)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1345..1369
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1627..1636
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1633..1635,1636..1638,1639..2349)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1633..1635,1636..1638,1639..2349)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1678..1699)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1939..1958)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2286..2307
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2649..2665)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2692..2716)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    2718..2825
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     2735..2751
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2785..2801)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2839..2859)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(2867..2883)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    2899..3033
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      4131..4140
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      4185..4194
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      4248..4257
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4254..4931
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             4254..4931
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(4384..4402)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4652..4671
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     4846..4869
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    join(5394..5581,5582..5593)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(5627..5643)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5680..5700)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5680..5698)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5719..5735)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5732..5754)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5743..5759
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5767..5773,5774..5791,5792..5797))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5812..5833
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5950..5967)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6121..6709)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6201..6220)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6880..7671,7672..7740))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7503..7522
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7741..7845)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa aggctggcta agaactcatc agcctccccg
      361 gtccatctac ccacgtacca atgcaccaat tggccacaat gacggctact acatggtgcc
      421 attccttcct ctttatagga atggagacta cctcctgtcc aacaatgctc ttggatacga
      481 gtacgcctac ctgttggacc caggtcattg cacaacacca gaaatgccct ctgatctgca
      541 aaagacgtga atatctgttc agacacccat atccactctg ttccacacag gtcagaggtt
      601 tgtccaggag ttcttgacag aggtgtaaaa agtactcaaa aattttactc aagtgaaagt
      661 acaagtactt agggaaaatt ttactcaatt aaaagtaaaa gtatctggct agaatcttac
      721 ttgagtaaaa gtaaaaaagt actccattaa aattgtactt gagtattaag gaagtaaaag
      781 taaaagcaag aaagaaaact agagattctt gtttaagctt ttaatctcaa aaaacattaa
      841 atgaaatgca tacaaggttt tatcctgctt tagaactgtt tgtatttaat tatcaaacta
      901 taagacagac aatctaatgc cagtacacgc tactcaaagt tgtaaaacct cagatttaac
      961 ttcagtagaa gctgattctc aaaattgtta gtgtcaagcc tagctctttt ggggctgaaa
     1021 agcaatcctg cagtgctgaa aagcctctca caggcagccg atgcgggaag aggtgtatta
     1081 gtcttgatag agaggctgca aatagcagga aacgtgagca gagactccct ggtgtctgaa
     1141 acacaggcca gatgggccct cgagcaggaa acagctatga ccatgattac gccaagctat
     1201 caactttgta tagaaaagtt gcaaaagctg ggtaccgggc cccccctcga ggtcgacggt
     1261 atcgataagc ttgatatcga attcctgcag cccgggggat ccactagttc tagagcggcc
     1321 gccaccgcgg tggagctcca attcgcaagt ttgtacaaaa aagcaggctc gaattggagc
     1381 tccaccgcgg tggcggccgc aaggataaac aattatcctc acgggtaatc gcttatcctc
     1441 aaggataaac aattatcctc acgggtaatc gcttatcctc aaggataaac aattatcctc
     1501 gcaagggtcg actctagagg gtatataatg gatcccatcg cgtctcagcc tcactttgag
     1561 ctcctccaca cgaattccct cgacctcgaa gacgcgtctg tttgggatcc accggtcact
     1621 agtgccgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     1681 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     1741 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     1801 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
     1861 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     1921 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     1981 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     2041 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     2101 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     2161 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     2221 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     2281 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     2341 ctgtacaaga gcaggcacag aaggcatcgc cagcgctcta ggagccgcaa tcgcagccga
     2401 agtcgcagca gtgaacgaaa acgccgtcaa cggagccgaa gtcgcagcag tgaacgaaga
     2461 cgctacttgt acaagtaatc tagaactata gtgagtcgta ttacgtagat ccagacatga
     2521 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
     2581 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
     2641 aagcttatcg ataccgtcga cctcgagggg gggcccggta cccagctttt gacccagctt
     2701 tcttgtacaa agtgggaggt accgggcccc ccctcgaggt cgacggtatc gataagcttg
     2761 atatcgaatt cctgcagccc gggggatcca ctagttctag agcggccgcc accgcggtgg
     2821 agctcaccgg tagatctgca actttattat acatagttga taattcactg gccgtcgttt
     2881 tacggtacca tcgatgatga tccagacatg ataagataca ttgatgagtt tggacaaacc
     2941 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
     3001 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg
     3061 tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt
     3121 ggtatggctg attatgatcc tctagatcag atcttgacca aagcttaaat cagttgtgtt
     3181 aaataagaga cattcaaaat aaatgtaaat gagctctcca aatcagcaga cttaacattc
     3241 tttaaaatga ttgattcaat agtgataaaa atcaggcata gccagttgta actttagata
     3301 aattacagaa aatgtcaaat acagagaacc gattcttttt tatgatacat ccaagcacac
     3361 atttaacaca atccaggcaa accccgaatt tcacagtcac aagcactgtt tgtacaagag
     3421 ctttgcctaa ggacacacag tctctataag tccaggtcgt tggtttcact cttattttaa
     3481 acatgtgaca tttttcctgc catcctgtct taggctgctg tttgcttcat tccatgtcac
     3541 attaatttcc tcagtagcac cttttacaca cacagccaat cttttccaga aaattcaatt
     3601 gctttgaaga gataatgtgt gaacaaatcc atttagaaaa ggaaaattaa gaatttgtaa
     3661 aatcatctgt aaattgttgg cattcttctg tatatgaaca tcacatcatt tacaggtaaa
     3721 ggtctggtca ttaattatat gacaatttac tggtattatt ttgtgaaagg ggctattttc
     3781 aatgcgttca tccatccttt tcatccctca aatctctcat tcacgtcccc ctccccatct
     3841 gcacacttta tctcattttc caccctgctg gaatctgagc acttgtgcag ttatcagggc
     3901 tcctgtattt aggaggctct gggtgtccat gtaggggacg aacagaaaca ctgcagacct
     3961 ttatagaaga acaaatgata agagtcctcg tacataaaga ctccattagt aacgtcagtg
     4021 acccaggagc ccagaccaac agcaaagcag acagtgaaat cactagtgaa ttcgcggccg
     4081 cctgcaggtc gacggtatcg attcgaattc aaggcctctc gagcctctaa gccaccatgg
     4141 gctgcatcaa gagcaagcgc aaggacaacc tgaacgacga cgaggccgcc atgggctgca
     4201 tcaagagcaa gcgcaaggac aacctgaacg acgacgaggc accggtcgcc accatggcct
     4261 cctccgagga cgtcatcaag gagttcatgc gcttcaaggt gcgcatggag ggctccgtga
     4321 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
     4381 ccgccaagct gaaggtgacc aagggcggcc ccctgccctt cgcctgggac atcctgtccc
     4441 ctcagttcca gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact
     4501 tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
     4561 gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga
     4621 agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct
     4681 gggaggcctc caccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaaga
     4741 tgaggctgaa gctgaaggac ggcggccact acgacgccga ggtcaagacc acctacatgg
     4801 ccaagaagcc cgtgcagctg cccggcgcct acaagaccga catcaagctg gacatcacct
     4861 cccacaacga ggactacacc atcgtggaac agtacgagcg cgccgagggc cgccactcca
     4921 ccggcgccta atacgtagat ccagacatga taagatacat tgatgagttt ggacaaacca
     4981 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
     5041 ttgtaaccat tataagctgc aataaacaag agatctgcga agatacggcc acgggtgctc
     5101 ttgatcctgt ggctgatttt ggactgtgct gctcgcagct gctgatgaat cacatacttc
     5161 ctccattttc ttccactgat tgactgttat aatttcccta atttccaggt caaggtgctg
     5221 tgcattgtgg taatagatgt gacatgacgt cacttccaaa ggaccaatga acatgtctga
     5281 ccaatttcat ataatgtgaa aacgattttc ataggcagaa taaataacat ttaaattaaa
     5341 ctgggcatca gcgcaattca attggtttgg taatagcaag ggaaaataga atgaagtgat
     5401 ctccaaaaaa taagtacttt ttgactgtaa ataaaattgt aaggagtaaa aagtactttt
     5461 ttttctaaaa aaatgtaatt aagtaaaagt aaaagtattg atttttaatt gtactcaagt
     5521 aaagtaaaaa tccccaaaaa taatacttaa gtacagtaat caagtaaaat tactcaagta
     5581 ctttacacct ctggttcttg accccctacc ttcagcaagc ccagcagatc cactagttct
     5641 agagcggccg ccaccgcggt ggagctccag cttttgttcc ctttagtgag ggttaattgc
     5701 gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     5761 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     5821 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     5881 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     5941 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     6001 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     6061 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     6121 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     6181 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     6241 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     6301 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     6361 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     6421 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     6481 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     6541 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     6601 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     6661 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     6721 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag gcattttggt
     6781 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     6841 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     6901 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     6961 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     7021 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     7081 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     7141 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     7201 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     7261 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     7321 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     7381 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     7441 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     7501 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     7561 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     7621 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     7681 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     7741 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     7801 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     7861 agtg
//
