LOCUS       sequence-31089-e         931 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 931)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PP-ARFGAP
FEATURES             Location/Qualifiers
     source          1..931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             40..57
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..90
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(580..598)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      594..641
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(816..821,822..838,839..844))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(870..888)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ctgaatattt tgtttacttt aagaaggaga tataccatgc atcatcatca tcatcacagc
       61 agcggcagag aaaacttgta tttccagggc atgaatattt caaatttaat taatgctgat
      121 gtagacgaaa aagggtttgt ttccgataaa ctcagagata atttcttcca aattgtgaga
      181 aataggccgg aaaatcgaac ttgctttgat tgtgaatcta ggaatcccac atggctttca
      241 ttatcatttg ctgtattcat ttgcctaaat tgttcctctg atcataggaa gatgggagta
      301 catattagtt ttgtaaggtc atctgatctt gataaattta ctccaattca gctggtgagg
      361 atggatattg gtggaaatgg tcgagcaaga aattatttca aacaagtttt aggagttaat
      421 ttttctccaa aaactaaaga gtacgccagt tccatatgtg ggagacaata caagcagata
      481 ctagattcag agatctctga atgatgacga agcttgcggc cgcactcgag gatccggctg
      541 ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat
      601 aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat
      661 ccggatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc
      721 cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg
      781 actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg
      841 tcaaacatga gaattcttga agacgaaggg cctcgtgata cgcctatttt tataggttaa
      901 tgtcatgata ataatggttt cttagacgtc a
//
