LOCUS       sequence-31094-e         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Nishino J, Kim I, Chada K, Morrison SJ
  TITLE     Hmga2 promotes neural stem cell self-renewal in young but not old 
            mice by reducing p16Ink4a and p19Arf Expression.
  JOURNAL   Cell. 2008 Oct 17. 135(2):227-39.
  PUBMED    18957199
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2008 
            Oct 17. 135(2):227-39."
COMMENT     Sequence Label: Let-7b-GFP
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     enhancer        239..542
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        543..746
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     697..717
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      785..794
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(836..857)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gtgttttgag actataaata tcccttggag aaaagccttg ttaacgcgcg gtgaccctcg
       61 aggggtgagg tagtaggttg tgtggtttca gggcagtgat gttgcccctc cgaagataac
      121 tatacaacct actgccttcc ctggcggccg cgtggataac cgtattaccg ccatgcatta
      181 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      241 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      301 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      361 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      421 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      481 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      541 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
      601 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      661 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
      721 ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc
      781 ggtcgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
      841 cgagctggac ggcgacgtaa acggccacna gttcagcgtg tccggcgagg g
//
