LOCUS       sequence-310942-        4294 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Part 3/3 for assembly of site-specific recombination based 
            Registers. Composed of the 5'UTR sequence of 35S promoter, coding 
            sequence of Luc and NOS terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4294)
  AUTHORS   Bernabe-Orts JM, Quijano-Rubio A, Vazquez-Vilar M, Mancheno-Bonillo 
            J, Moles-Casas V, Selma S, Gianoglio S, Granell A, Orzaez D
  TITLE     A memory switch for plant synthetic biology based on the phage 
            varphiC31 integration system.
  JOURNAL   Nucleic Acids Res. 2020 Apr 6;48(6):3379-3394. doi: 
            10.1093/nar/gkaa104.
  PUBMED    32083668
REFERENCE   2  (bases 1 to 4294)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2020-04-6- 6"; volume: "48"; issue: "6";
            pages: "3379-3394"
COMMENT     Sequence Label: pUPD2 Assembly Register 3/3 Part - 5'UTR:Luc:T...
FEATURES             Location/Qualifiers
     source          1..4294
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     370..389
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      complement(639..682)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    685..706
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     CDS             759..2411
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(825..843)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      2438..2690
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      2952..3009
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(3033..3050)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3204..3792)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3284..3303)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3975..4069)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(4090..4294,1..455))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 ctcacccagg gattggctga tacgaaaaac atattctcaa taaacccttt agggaaatag
       61 gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa
      121 tcgtcgtggt attcactcca gagtgatgaa aacgtttcag tttgctcatg gaaaacggtg
      181 taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc catacgaaat
      241 tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc
      301 ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag
      361 gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata
      421 tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat
      481 ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa
      541 cctcttacgt gcccgatcaa ctcgagtgcc acctgacgtc taagaaacca ttattatcat
      601 gacattaacc tataaaaata ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt
      661 agctttcgct aaggatgatt tctggaattc gcggccgctt ctagagcgat gagtggtctc
      721 gaccttaaac aacattacaa tttactattc tagtcgaaat ggaagacgcc aaaaacataa
      781 agaaaggccc ggcgccattc tatccgctgg aagatggaac cgctggagag caactgcata
      841 aggctatgaa gagatacgcc ctggttcctg gaacaattgc ttttacagat gcacatatcg
      901 aggtggacat cacttacgct gagtacttcg aaatgtccgt tcggttggca gaagctatga
      961 aacgatatgg gctgaataca aatcacagaa tcgtcgtatg cagtgaaaac tctcttcaat
     1021 tctttatgcc ggtgttgggc gcgttattta tcggagttgc agttgcgccc gcgaacgaca
     1081 tttataatga acgtgaattg ctcaacagta tgggcatttc gcagcctacc gtggtgttcg
     1141 tttccaaaaa ggggttgcaa aaaattttga acgtgcaaaa aaagctccca atcatccaaa
     1201 aaattattat catggattct aaaacggatt accagggatt tcagtcgatg tacacgttcg
     1261 tcacatctca tctacctccc ggttttaatg aatacgattt tgtgccagag tccttcgata
     1321 gggacaagac aattgcactg atcatgaact cctctggatc tactggtctg cctaaaggtg
     1381 tcgctctgcc tcatagaact gcctgcgtga gattctcgca tgccagagat cctatttttg
     1441 gcaatcaaat cattccggat actgcgattt taagtgttgt tccattccat cacggttttg
     1501 gaatgtttac tacactcgga tatttgatat gtggatttcg agtcgtctta atgtatagat
     1561 ttgaagaaga gctgtttctg aggagccttc aggattacaa gattcaaagt gcgctgctgg
     1621 tgccaaccct attctccttc ttcgccaaaa gcactctgat tgacaaatac gatttatcta
     1681 atttacacga aattgcttct ggtggcgctc ccctctctaa ggaagtcggg gaagcggttg
     1741 ccaagaggtt ccatctgcca ggtatcaggc aaggatatgg gctcactgag actacatcag
     1801 ctattctgat tacacccgag ggggatgata aaccgggcgc ggtcggtaaa gttgttccat
     1861 tttttgaagc gaaggttgtg gatctggata ccgggaaaac gctgggcgtt aatcaaagag
     1921 gcgaactgtg tgtgagaggt cctatgatta tgtccggtta tgtaaacaat ccggaagcga
     1981 ccaacgcctt gattgacaag gatggatggc tacattctgg agacatagct tactgggacg
     2041 aagacgaaca cttcttcatc gttgaccgcc tgaagtctct gattaagtac aaaggctatc
     2101 aggtggctcc cgctgaattg gaatccatct tgctccaaca ccccaacatc ttcgacgcag
     2161 gtgtcgcagg tcttcccgac gatgacgccg gtgaacttcc cgccgccgtt gttgttttgg
     2221 agcacggaaa gacgatgacg gaaaaagaga tcgtggatta cgtcgccagt caagtaacaa
     2281 ccgcgaaaaa gttgcgcgga ggagttgtgt ttgtggacga agtaccgaaa ggtcttaccg
     2341 gaaaactcga cgcaagaaaa atcagagaga tcctcataaa ggccaagaag ggcggaaaga
     2401 tcgccgtgta atgagcttgg aatggatctt cgatcccgat cgttcaaaca tttggcaata
     2461 aagtttctta agattgaatc ctgttgccgg tcttgcgacg attatcatat aatttctgtt
     2521 gaattacgtt aagcatgtaa taattaacat gtaatgcatg acgttattta tgagatgggt
     2581 ttttatgatt agagtcccgc aattatacat ttaatacgcg atagaaaaca aaatatagcg
     2641 cgcaaactag gataaattat cgcgcdcggt gtcatctatg ttactagatc gggaattgcc
     2701 aagctaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     2761 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     2821 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     2881 gataaatgct tcaataatgg gaccgactcg cgcttgagac cactcatcgc gactagtagc
     2941 ggccgctgca gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt ttctttaaaa
     3001 ccgaaaagat tacttcgcgt tatgcaggct tcctcgctca ctgactcgct gcgctcggtc
     3061 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     3121 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     3181 aaaaaggccg cgttgctggc gtttttccac aggctccgcc cccctgacga gcatcacaaa
     3241 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     3301 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     3361 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     3421 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     3481 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     3541 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     3601 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     3661 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     3721 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     3781 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     3841 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     3901 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     3961 agctcgaggc ttggattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac
     4021 aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc
     4081 gatatcaaat tacgccccgc cctgccactc gtcgcagtac tgttgtaatt cattaagcat
     4141 tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag
     4201 caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc
     4261 catattggcc acgtttaaat caaaactggt gaaa
//
