LOCUS       sequence-310958-        2498 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CDS of MS2 protein with B3 nomenclature.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2498)
  TITLE     Orzaez lab plasmids
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pUPD2 MS2 (GB2665)
FEATURES             Location/Qualifiers
     source          1..2498
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             5..391
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2
                     /note="binds to a specific stem-loop structure in the viral
                     RNA (Peabody, 1993)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQNRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     terminator      435..492
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(516..533)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(687..1275)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(767..786)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1458..1552)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1573..2232)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2147..2166
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2394..2412
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2416..2459)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2462..2483
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 aatggcttct aattttactc aatttgttct tgttgataat ggtggtactg gtgatgttac
       61 tgttgctcct tctaattttg ctaatggtgt tgctgaatgg atttcttcta attctagatc
      121 tcaagcttat aaggttactt gttctgttag acaatcttct gctcaaaata gaaagtatac
      181 tattaaggtt gaagttccta aggttgctac tcaaactgtt ggtggtgttg aacttcctgt
      241 tgctgcttgg agatcttatc ttaatatgga acttactatt cctatttttg ctactaattc
      301 tgattgtgaa cttattgtta aggctatgca aggtcttctt aaggatggta atcctattcc
      361 ttctgctatt gctgctaatt ctggtattta tgcagcctga gaccactcat cgcgactagt
      421 agcggccgct gcagtccggc aaaaaagggc aaggtgtcac caccctgccc tttttcttta
      481 aaaccgaaaa gattacttcg cgttatgcag gcttcctcgc tcactgactc gctgcgctcg
      541 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
      601 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
      661 cgtaaaaagg ccgcgttgct ggcgtttttc cacaggctcc gcccccctga cgagcatcac
      721 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
      781 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
      841 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
      901 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
      961 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     1021 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     1081 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     1141 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     1201 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     1261 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     1321 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     1381 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     1441 gacagctcga ggcttggatt ctcaccaata aaaaacgccc ggcggcaacc gagcgttctg
     1501 aacaaatcca gatggagttc tgaggtcatt actggatcta tcaacaggag tccaagcgag
     1561 ctcgatatca aattacgccc cgccctgcca ctcgtcgcag tactgttgta attcattaag
     1621 cattctgccg acatggaagc catcacaaac ggcatgatga acctgaatcg ccagcggcat
     1681 cagcaccttg tcgccttgcg tataatattt gcccatggtg aaaacggggg cgaagaagtt
     1741 gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat tggctgatac
     1801 gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac cgtaacacgc
     1861 cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag
     1921 tgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca
     1981 tatcaccagc tcaccgtctt tcattgccat acgaaattcc ggatgagcat tcatcaggcg
     2041 ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa
     2101 aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa
     2161 tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat
     2221 ttttttctcc attttagctt ccttagctcc tgaaaatctc gataactcaa aaaatacgcc
     2281 cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc cgatcaactc
     2341 gagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     2401 gtatcacgag gcagaatttc agataaaaaa aatccttagc tttcgctaag gatgatttct
     2461 ggaattcgcg gccgcttcta gagcgatgag tggtctcg
//
