LOCUS       sequence-31096-e         905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Nishino J, Kim I, Chada K, Morrison SJ
  TITLE     Hmga2 promotes neural stem cell self-renewal in young but not old 
            mice by reducing p16Ink4a and p19Arf Expression.
  JOURNAL   Cell. 2008 Oct 17. 135(2):227-39.
  PUBMED    18957199
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2008 
            Oct 17. 135(2):227-39."
COMMENT     Sequence Label: HMGA2+GFP
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     406..422
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     407..423
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(465..498)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tccagctgga attcgagctc ggtaccctcg aggcggcggg aggcaggatg agcgcacgcg
       61 gtgagggcgc cgggcagccg tccacatcag cccagggaca acctgccgcc ccggtgccac
      121 agaagcgagg acgcggccga cccaggaagc agcagcaaga gccaacctgt gagccctctc
      181 ctaagagacc cagaggaaga cccaaaggca gcaaaaacaa gagcccctct aaagcagccc
      241 agaagaaagc agagaccatt ggagaaaaac ggccaagagg cagacctagg aaatggccac
      301 aacaagtcgt tcagaagaag cctgctcagg agactgaaga gacatcctcg caagagtccg
      361 cagaggagga ttaggaattc ctgcagcccg ggggatcctc tagagtcgag gtcgacggta
      421 tcgataagct cgcttcacga gattccagca ggtcgaggga cctaataact tcgtatagca
      481 tacattatac gaagttatat taagggttcc aagcttaagc ggccgcgtgg ataaccgtat
      541 taccgccatg cattagttat taatagtaat caattacggg gtcattagtt catagcccat
      601 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      661 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      721 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      781 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      841 attatgccca gtacatgacc ttatgggact ttcctacttg gnagtacatc tacgtattag
      901 tcatc
//
