LOCUS       sequence-310963-        2278 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2278)
  TITLE     Orzaez lab plasmids
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pUPD2 Etr4 (GB2719)
FEATURES             Location/Qualifiers
     source          1..2278
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     223..242
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     470..488
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(492..535)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    538..559
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      789..846
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(870..887)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1041..1629)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1121..1140)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1812..1906)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(1927..2278,1..308))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc
       61 accagctcac cgtctttcat tgccatacga aattccggat gagcattcat caggcgggca
      121 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag
      181 gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc
      241 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt
      301 ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt
      361 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgcccgat caactcgagt
      421 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
      481 cacgaggcag aatttcagat aaaaaaaatc cttagctttc gctaaggatg atttctggaa
      541 ttcgcggccg cttctagagc gatgagtggt ctcgggagaa aagcttcgaa gtttaaacga
      601 ttgaatataa ccgacgtgac tgttacattt agggattgaa tataaccgac gtgactgtta
      661 catttaggga ttgaatataa ccgacgtgac tgttacattt agggattgaa tataaccgac
      721 gtgactgtta catttaggga tgctagctcc ctgagaccac tcatcgcgac tagtagcggc
      781 cgctgcagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc tttaaaaccg
      841 aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg ctcggtcgtt
      901 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
      961 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     1021 aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca tcacaaaaat
     1081 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     1141 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     1201 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     1261 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     1321 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     1381 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     1441 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     1501 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     1561 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     1621 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     1681 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     1741 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagc
     1801 tcgaggcttg gattctcacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa
     1861 tccagatgga gttctgaggt cattactgga tctatcaaca ggagtccaag cgagctcgat
     1921 atcaaattac gccccgccct gccactcgtc gcagtactgt tgtaattcat taagcattct
     1981 gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac
     2041 cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat
     2101 attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg atacgaaaaa
     2161 catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc
     2221 ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagtgat
//
