LOCUS       sequence-31097-e         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Nishino J, Kim I, Chada K, Morrison SJ
  TITLE     Hmga2 promotes neural stem cell self-renewal in young but not old 
            mice by reducing p16Ink4a and p19Arf Expression.
  JOURNAL   Cell. 2008 Oct 17. 135(2):227-39.
  PUBMED    18957199
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2008 
            Oct 17. 135(2):227-39."
COMMENT     Sequence Label: HMGA2(3'UTR DEL)+GFP
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     414..430
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     415..431
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(473..506)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gtgcacctcc nagctggaat tcgagctcgg taccctcgag gcggcgggag gcaggatgag
       61 cgcacgcggt gagggcgccg ggcagccgtc cacatcagcc cagggacaac ctgccgcccc
      121 ggtgccacag aagcgaggac gcggccgacc caggaagcag cagcaagagc caacctgtga
      181 gccctctcct aagagaccca gaggaagacc caaaggcagc aaaaacaaga gcccctctaa
      241 agcagcccag aagaaagcag agaccattgg agaaaaacgg ccaagaggca gacctaggaa
      301 atggccacaa caagtcgttc agaagaagcc tgctcaggag actgaagaga catcctcgca
      361 agagtccgca gaggaggatt aggaattcct gcagcccggg ggatcctcta gagtcgaggt
      421 cgacggtatc gataagctcg cttcacgaga ttccagcagg tcgagggacc taataacttc
      481 gtatagcata cattatacga agttatatta agggttccaa gcttaagcgg ccgcgtggat
      541 aaccgtatta ccgccatgca ttagttatta atagtaatca attacggggt cattagttca
      601 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
      661 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
      721 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
      781 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
      841 cgcctggcat tatgccatnt gctngattc
//
