LOCUS       sequence-310972-       11368 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transcriptional unit for the expression of the LbCas12a in plant 
            systems driven by the CaMV35s promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11368)
  AUTHORS   Bernabe-Orts JM, Casas-Rodrigo I, Minguet EG, Landolfi V, 
            Garcia-Carpintero V, Gianoglio S, Vazquez-Vilar M, Granell A, Orzaez
            D
  TITLE     Assessment of Cas12a-mediated gene editing efficiency in plants.
  JOURNAL   Plant Biotechnol J. 2019 Apr 5. doi: 10.1111/pbi.13113.
  PUBMED    30950179
REFERENCE   2  (bases 1 to 11368)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2019 Apr 5. doi: 10.1111/pbi.13113."
COMMENT     Sequence Label: pDGB3 alpha1 P35S:LbCas12a:T35S (GB1441)
FEATURES             Location/Qualifiers
     source          1..11368
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        616..960
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     904..924
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1033..4716
                     /codon_start=1
                     /product="CRISPR-associated protein from Lachnospiraceae 
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /label=LbCpf1
                     /translation="MSKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAE
                     DYKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKE
                     IAKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFS
                     EEAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEG
                     EFFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVL
                     SDRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKN
                     GPAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQ
                     LQEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKD
                     LLDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYS
                     KDKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNG
                     NYEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKL
                     IDFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLV
                     EEGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKK
                     EELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKI
                     NTEVRVLLKHDDNPYVIGIDRGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTD
                     YHSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSG
                     FKNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQ
                     NGFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALD
                     YKNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQ
                     QGDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRN
                     YEAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTS
                     VKH"
     CDS             4717..4764
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     misc_feature    5018..5042
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6343..6972
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIGTHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7406..8473
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      8539..8733
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(8969..8991)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9077..9217
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9132..9151
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9232..9249)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9403..9991)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9483..9502)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10079..10894)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(10079..10894)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     10802..10821
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    11327..11351
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ggagactaga gccaagctga tctcctttgc cccggagatc accatggacg actttctcta
       61 tctctacgat ctaggaagaa agttcgacgg agaaggtgac gataccatgt tcaccaccga
      121 taatgagaag attagcctct tcaatttcag aaagaatgct gacccacaga tggttagaga
      181 ggcctacgcg gcaggtctga tcaagacgat ctacccgagt aataatctcc aggagatcaa
      241 ataccttccc aagaaggtta aagatgcagt caaaagattc aggactaact gcatcaagaa
      301 cacagagaaa gatatatttc tcaagatcag aagtactatt ccagtatgga cgattcaagg
      361 cttgcttcat aaaccaaggc aagtaataga gattggagtc tctaagaaag tagttcctac
      421 tgaatcaaag gccatggagt caaaaattca gatcgaggat ctaacagaac tcgccgtgaa
      481 gactggcgaa cagttcatac agagtctttt acgactcaat gacaagaaga aaatcttcgt
      541 caacatggtg gagcacgaca ctctcgtcta ctccaagaat atcaaagata cagtctcaga
      601 agaccaaagg gctattgaga cttttcaaca aagggtaata tcgggaaacc tcctcggatt
      661 ccattgccca gctatctgtc acttcatcaa aaggacagta gaaaaggaag gtggcaccta
      721 caaatgccat cattgcgata aaggaaaggc tatcgttcaa gatgcctctg ccgacagtgg
      781 tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac
      841 gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg acgcacaatc
      901 ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac
      961 tccggtattt ttacaacaat accacaacaa aacaaacaac aaacaacatt acaatttact
     1021 attctagtcg aaatgagcaa gctggagaag tttacaaact gctactccct gtctaagacc
     1081 ctgaggttca aggccatccc tgtgggcaag acccaggaga acatcgacaa taagcggctg
     1141 ctggtggagg acgagaagag agccgaggat tataagggcg tgaagaagct gctggatcgc
     1201 tactatctgt cttttatcaa cgacgtgctg cacagcatca agctgaagaa tctgaacaat
     1261 tacatcagcc tgttccggaa gaaaaccaga accgagaagg agaataagga gctggagaac
     1321 ctggagatca atctgcggaa ggagatcgcc aaggccttca agggcaacga gggctacaag
     1381 tccctgttta agaaggatat catcgagaca atcctgccag agttcctgga cgataaggac
     1441 gagatcgccc tggtgaacag cttcaatggc tttaccacag ccttcaccgg cttctttgat
     1501 aacagagaga atatgttttc cgaggaggcc aagagcacat ccatcgcctt caggtgtatc
     1561 aacgagaatc tgacccgcta catctctaat atggacatct tcgagaaggt ggacgccatc
     1621 tttgataagc acgaggtgca ggagatcaag gagaagatcc tgaacagcga ctatgatgtg
     1681 gaggatttct ttgagggcga gttctttaac tttgtgctga cacaggaggg catcgacgtg
     1741 tataacgcca tcatcggcgg cttcgtgacc gagagcggcg agaagatcaa gggcctgaac
     1801 gagtacatca acctgtataa tcagaaaacc aagcagaagc tgcctaagtt taagccactg
     1861 tataagcagg tgctgagcga tcgggagtct ctgagcttct acggcgaggg ctatacatcc
     1921 gatgaggagg tgctggaggt gtttagaaac accctgaaca agaacagcga gatcttcagc
     1981 tccatcaaga agctggagaa gctgttcaag aattttgacg agtactctag cgccggcatc
     2041 tttgtgaaga acggccccgc catcagcaca atctccaagg atatcttcgg cgagtggaac
     2101 gtgatccggg acaagtggaa tgccgagtat gacgatatcc acctgaagaa gaaggccgtg
     2161 gtgaccgaga agtacgagga cgatcggaga aagtccttca agaagatcgg ctccttttct
     2221 ctggagcagc tgcaggagta cgccgacgcc gatctgtctg tggtggagaa gctgaaggag
     2281 atcatcatcc agaaggtgga tgagatctac aaggtgtatg gctcctctga gaagctgttc
     2341 gacgccgatt ttgtgctgga gaagagcctg aagaagaacg acgccgtggt ggccatcatg
     2401 aaggacctgc tggattctgt gaagagcttc gagaattaca tcaaggcctt ctttggcgag
     2461 ggcaaggaga caaacaggga cgagtccttc tatggcgatt ttgtgctggc ctacgacatc
     2521 ctgctgaagg tggaccacat ctacgatgcc atccgcaatt atgtgaccca gaagccctac
     2581 tctaaggata agttcaagct gtattttcag aaccctcagt tcatgggcgg ctgggacaag
     2641 gataaggaga cagactatcg ggccaccatc ctgagatacg gctccaagta ctatctggcc
     2701 atcatggata agaagtacgc caagtgcctg cagaagatcg acaaggacga tgtgaacggc
     2761 aattacgaga agatcaacta taagctgctg cccggcccta ataagatgct gccaaaggtg
     2821 ttcttttcta agaagtggat ggcctactat aaccccagcg aggacatcca gaagatctac
     2881 aagaatggca cattcaagaa gggcgatatg tttaacctga atgactgtca caagctgatc
     2941 gacttcttta aggatagcat ctcccggtat ccaaagtggt ccaatgccta cgatttcaac
     3001 ttttctgaga cagagaagta taaggacatc gccggctttt acagagaggt ggaggagcag
     3061 ggctataagg tgagcttcga gtctgccagc aagaaggagg tggataagct ggtggaggag
     3121 ggcaagctgt atatgttcca gatctataac aaggactttt ccgataagtc tcacggcaca
     3181 cccaatctgc acaccatgta cttcaagctg ctgtttgacg agaacaatca cggacagatc
     3241 aggctgagcg gaggagcaga gctgttcatg aggcgcgcct ccctgaagaa ggaggagctg
     3301 gtggtgcacc cagccaactc ccctatcgcc aacaagaatc cagataatcc caagaaaacc
     3361 acaaccctgt cctacgacgt gtataaggat aagaggtttt ctgaggacca gtacgagctg
     3421 cacatcccaa tcgccatcaa taagtgcccc aagaacatct tcaagatcaa tacagaggtg
     3481 cgcgtgctgc tgaagcacga cgataacccc tatgtgatcg gcatcgatag gggcgagcgc
     3541 aatctgctgt atatcgtggt ggtggacggc aagggcaaca tcgtggagca gtattccctg
     3601 aacgagatca tcaacaactt caacggcatc aggatcaaga cagattacca ctctctgctg
     3661 gacaagaagg agaaggagag gttcgaggcc cgccagaact ggacctccat cgagaatatc
     3721 aaggagctga aggccggcta tatctctcag gtggtgcaca agatctgcga gctggtggag
     3781 aagtacgatg ccgtgatcgc cctggaggac ctgaactctg gctttaagaa tagccgcgtg
     3841 aaggtggaga agcaggtgta tcagaagttc gagaagatgc tgatcgataa gctgaactac
     3901 atggtggaca agaagtctaa tccttgtgca acaggcggcg ccctgaaggg ctatcagatc
     3961 accaataagt tcgagagctt taagtccatg tctacccaga acggcttcat cttttacatc
     4021 cctgcctggc tgacatccaa gatcgatcca tctaccggct ttgtgaacct gctgaaaacc
     4081 aagtatacca gcatcgccga ttccaagaag ttcatcagct cctttgacag gatcatgtac
     4141 gtgcccgagg aggatctgtt cgagtttgcc ctggactata agaacttctc tcgcacagac
     4201 gccgattaca tcaagaagtg gaagctgtac tcctacggca accggatcag aatcttccgg
     4261 aatcctaaga agaacaacgt gttcgactgg gaggaggtgt gcctgaccag cgcctataag
     4321 gagctgttca acaagtacgg catcaattat cagcagggcg atatcagagc cctgctgtgc
     4381 gagcagtccg acaaggcctt ctactctagc tttatggccc tgatgagcct gatgctgcag
     4441 atgcggaaca gcatcacagg ccgcaccgac gtggattttc tgatcagccc tgtgaagaac
     4501 tccgacggca tcttctacga tagccggaac tatgaggccc aggagaatgc catcctgcca
     4561 aagaacgccg acgccaatgg cgcctataac atcgccagaa aggtgctgtg ggccatcggc
     4621 cagttcaaga aggccgagga cgagaagctg gataaggtga agatcgccat ctctaacaag
     4681 gagtggctgg agtacgccca gaccagcgtg aagcacaaaa ggccggcggc cacgaaaaag
     4741 gccggccagg caaaaaagaa aaagtaggct tcggccatgc tagagtccgc aaaaatcacc
     4801 agtctctctc tacaaatcta tctctctcta tttttctcca gaataatgtg tgagtagttc
     4861 ccagataagg gaattagggt tcttataggg tttcgctcat gtgttgagca tataagaaac
     4921 ccttagtatg tatttgtatt tgtaaaatac ttctatcaat aaaatttcta attcctaaaa
     4981 ccaaaatcca gtgacctcgc tgtcatgaga cgaattctga caggatatat tggcgggtaa
     5041 acctaagaga aaagagcgtt tattagaata atcggatatt taaaagggcg tgaaaaggtt
     5101 tatccgttcg tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg gatcaaagta
     5161 ctttgatcca acccctccgc tgctatagtg cagtcggctt ctgacgttca gtgcagccgt
     5221 catctgaaaa cgacatgtcg cacaagtcct aagttacgcg acaggctgcc gccctgccct
     5281 tttcctggcg ttttcttgtc gcgtgtttta gtcgcataaa gtagaatact tgcgactaga
     5341 accggagaca ttacgccatg aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg
     5401 tcagcaccga cgaccaggac ttgaccaacc aacgggccga actgcacgcg gccggctgca
     5461 ccaagctgtt ttccgagaag atcaccggca ccaggcgcga ccgcccggag ctggccagga
     5521 tgcttgacca cctacgccct ggcgacgttg tgacagtgac caggctagac cgcctggccc
     5581 gcagcacccg cgacctactg gacattgccg agcgcatcca ggaggccggc gcgggcctgc
     5641 gtagcctggc agagccgtgg gccgacacca ccacgccggc cggccgcatg gtgttgaccg
     5701 tgttcgccgg cattgccgag ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc
     5761 gcgaggccgc caaggcccga ggcgtgaagt ttggcccccg ccctaccctc accccggcac
     5821 agatcgcgca cgcccgcgag ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg
     5881 cactgcttgg cgtgcatcgc tcgaccctgt accgcgcact tgagcgcagc gaggaagtga
     5941 cgcccaccga ggccaggcgg cgcggtgcct tccgtgagga cgcattgacc gaggccgacg
     6001 ccctggcggc cgccgagaat gaacgccaag aggaacaagc atgaaaccgc accaggacgg
     6061 ccaggacgaa ccgtttttca ttaccgaaga gatcgaggcg gagatgatcg cggccgggta
     6121 cgtgttcgag ccgcccgcgc acctctcaac cgtgcggctg catgaaatcc tggccggttt
     6181 gtctgatgcc aagctggcgg cctggccggc cagcttggcc gctgaagaaa ccgagcgccg
     6241 ccgtctaaaa aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta
     6301 tatgatccga tgagtaaata aacaaatacg caaggggaac gcatgaaggt tatcgctgta
     6361 cttaaccaga aaggcgggtc aggcaagacg accatcggaa cccatctagc ccgcgccctg
     6421 caactcgccg gggccgatgt tctgttagtc gattccgatc cccagggcag tgcccgcgat
     6481 tgggcggccg tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt
     6541 gaccgcgacg tgaaggccat cggccggcgc gacttcgtag tgatcgacgg agcgccccag
     6601 gcggcggact tggctgtgtc cgcgatcaag gcagccgact tcgtgctgat tccggtgcag
     6661 ccaagccctt acgacatatg ggccaccgcc gacctggtgg agctggttaa gcagcgcatt
     6721 gaggtcacgg atggaaggct acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg
     6781 cgcatcggcg gtgaggttgc cgaggcgctg gccgggtacg agctgcccat tcttgagtcc
     6841 cgtatcacgc agcgcgtgag ctacccaggc actgccgccg ccggcacaac cgttcttgaa
     6901 tcagaacccg agggcgacgc tgcccgcgag gtccaggcgc tggccgctga aattaaatca
     6961 aaactcattt gagttaatga ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg
     7021 ccggccgtcc gagcgcacgc agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc
     7081 cagccatgaa gcgggtcaac tttcagttgc cggcggagga tcacaccaag ctgaagatgt
     7141 acgcggtacg ccaaggcaag accattaccg agctgctatc tgaatagatc gcgcagctac
     7201 cagagtaaat gagcaaatga ataaatgagt agatgaattt tagcggctaa aggaggcggc
     7261 atggaaaatc aagaacaacc aggcaccgac gccgtggaat gccccatgtg tggaggaacg
     7321 ggcggttggc caggcgtaag cggctgggtt gtctgccggc cctgcaatgg cactggaacc
     7381 cccaagcccg aggaatcggc gtgacggtcg caaaccatcc ggcccggtac aaatcggcgc
     7441 ggcgctgggt gatgacctgg tggagaagtt gaaggccgcg caggccgccc agcggcaacg
     7501 catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc gaatccgcaa
     7561 agaatcccgg caaccgccgg cagccggtgc gccgtcgatt aggaagccgc ccaagggcga
     7621 cgagcaacca gattttttcg ttccgatgct ctatgacgtg ggcacccgcg atagtcgcag
     7681 catcatggac gtggccgttt tccgtctgtc gaagcgtgac cgacgagctg gcgaggtgat
     7741 ccgctacgag cttccagacg ggcacgtaga ggtttccgca gggccggccg gcatggccag
     7801 tgtgtgggat tacgacctgg tactgatggc ggtttcccat ctaaccgaat ccatgaaccg
     7861 ataccgggaa gggaagggag acaagcccgg ccgcgtgttc cgtccacacg ttgcggacgt
     7921 actcaagttc tgccggcgag ccgatggcgg aaagcagaaa gacgacctgg tagaaacctg
     7981 cattcggtta aacaccacgc acgttgccat gcagcgtacg aagaaggcca agaacggccg
     8041 cctggtgacg gtatccgagg gtgaagcctt gattagccgc tacaagatcg taaagagcga
     8101 aaccgggcgg ccggagtaca tcgagatcga gctagctgat tggatgtacc gcgagatcac
     8161 agaaggcaag aacccggacg tgctgacggt tcaccccgat tactttttga tcgatcccgg
     8221 catcggccgt tttctctacc gcctggcacg ccgcgccgca ggcaaggcag aagccagatg
     8281 gttgttcaag acgatctacg aacgcagtgg cagcgccgga gagttcaaga agttctgttt
     8341 caccgtgcgc aagctgatcg ggtcaaatga cctgccggag tacgatttga aggaggaggc
     8401 ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac ctgatcgagg gcgaagcatc
     8461 cgccggttcc taatgtacgg agcagatgct agggcaaatt gccctagcag gggaaaaagg
     8521 tcgaaaagga ctctttcctg tggatagcac gtacattggg aacccaaagc cgtacattgg
     8581 gaaccggaac ccgtacattg ggaacccaaa gccgtacatt gggaaccggt cacacatgta
     8641 agtgactgat ataaaagaga aaaaaggcga tttttccgcc taaaactctt taaaacttat
     8701 taaaactctt aaaacccgcc tggcctgtgc ataactgtct ggccagcgca cagccgaaga
     8761 gctgcaaaaa gcgcctaccc ttcggtcgct gcgctcccta cgccccgccg cttcgcgtcg
     8821 gcctatcgcg gccgctggcc gctcaaaaat ggctggccta cggccaggca atctaccagg
     8881 gcgcggacaa gccgcgccgt cgccactcga ccgccggcgc ccacatcaag gcaccctgcc
     8941 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gtgacggtca
     9001 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     9061 ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg
     9121 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
     9181 accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac
     9241 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     9301 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     9361 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     9421 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     9481 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     9541 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     9601 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     9661 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     9721 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     9781 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     9841 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     9901 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     9961 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
    10021 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgcatt ctaggtgatt
    10081 agaaaaactc atcgagcatc aaatgaaact gcaatttatt catatcagga ttatcaatac
    10141 catatttttg aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg cagttccata
    10201 ggatggcaag atcctggtat cggtctgcga ttccgactcg tccaacatca atacaaccta
    10261 ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa atcaccatga gtgacgactg
    10321 aatccggtga gaatggcaaa agtttatgca tttctttcca gacttgttca acaggccagc
    10381 cattacgctc gtcatcaaaa tcactcgcat caaccaaacc gttattcatt cgtgattgcg
    10441 cctgagcgag tcgaaatacg cgatcgctgt taaaaggaca attacaaaca ggaatcgaat
    10501 gcaaccggcg caggaacact gccagcgcat caacaatatt ttcacctgaa tcaggatatt
    10561 cttctaatac ctggaatgct gttttccctg ggatcgcagt ggtgagtaac catgcatcat
    10621 caggagtacg gataaaatgc ttgatggtcg gaagaggcat aaattccgtc agccagttta
    10681 gtctgaccat ctcatctgta acatcattgg caacgctacc tttgccatgt ttcagaaaca
    10741 actctggcgc atcgggcttc ccatacaatc ggtagattgt cgcacctgat tgcccgacat
    10801 tatcgcgagc ccatttatac ccatataaat cagcatccat gttggaattt aatcgcggcc
    10861 ttgagcaaga cgtttcccgt tgaatatggc tcataacaga acttattatt tccttcctct
    10921 tttctacagt atttaaagat accccaagaa gctaattata acaagacgaa ctccaattca
    10981 ctgttccttg cattctaaaa ccttaaatac cagaaaacag ctttttcaaa gttgttttca
    11041 aagttggcgt ataacatagt atcgacggag ccgattttga aaccgcggtg atcacaggca
    11101 gcaacgctct gtcatcgtta caatcaacat gctaccctcc gcgagatcat ccgtgtttca
    11161 aacccggcag cttagttgcc gttcttccga atagcatcgg taacatgagc aaagtctgcc
    11221 gccttacaac ggctctcccg ctgacgccgt cccggactga tgggctgcct gtatcgagtg
    11281 gtgattttgt gccgagctgc cggtcgggga gctgttggct ggctggtggc aggatatatt
    11341 gtggtgtaaa cataacgaat tcgtctca
//
