LOCUS       sequence-310977-        9002 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TU for the constitutive expression of Streptomyces phage PhiC31 
            integrase..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9002)
  AUTHORS   Bernabe-Orts JM, Quijano-Rubio A, Vazquez-Vilar M, Mancheno-Bonillo 
            J, Moles-Casas V, Selma S, Gianoglio S, Granell A, Orzaez D
  TITLE     A memory switch for plant synthetic biology based on the phage 
            varphiC31 integration system.
  JOURNAL   Nucleic Acids Res. 2020 Apr 6;48(6):3379-3394. doi: 
            10.1093/nar/gkaa104.
  PUBMED    32083668
REFERENCE   2  (bases 1 to 9002)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2020-04-6- 6"; volume: "48"; issue: "6";
            pages: "3379-3394"
COMMENT     Sequence Label: pEGB3 alpha1 Pnos:PhiC31int:Tnos (GB1531)
FEATURES             Location/Qualifiers
     source          1..9002
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        83..262
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             297..2111
                     /codon_start=1
                     /product="integrase from phage phi-C31"
                     /label=phage phi-C31 integrase
                     /translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG
                     GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP
                     IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
                     GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
                     IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
                     RDPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
                     WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
                     QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
                     NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
                     APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
                     EKRASITWAKPPTDDDEDDAQDGTEDVAA"
     CDS             2112..2132
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      2159..2411
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2446..2464)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    2652..2676
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3977..4606
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIGTHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5040..6107
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      6173..6367
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(6603..6625)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6711..6851
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6766..6785
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6866..6883)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7037..7625)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7117..7136)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7713..8528)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(7713..8528)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8436..8455
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    8961..8985
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ggagatacat gagaattaag ggagtcacgg tatgaccccc gccgatgacg cgggacaagc
       61 cgttttacgt ttggaactga cagaaccgca acgttgaagg agccactgag ccgcgggttt
      121 ctggagttta atgagctaag cacatacgtc agaaaccatt attgcgcgtt caaaagtcgc
      181 ctaaggtcac tatcagctag caaatatttc ttgtcaaaaa tgctccactg acgttccata
      241 aattcccctc ggtatccaat tagagtctca tattcactct caactcgatc gaggcaatgg
      301 acacgtatgc cggcgcttat gatagacaat caagagaaag agagaactct tctgccgcct
      361 ctccagctac acagagatct gccaacgagg ataaagctgc tgatttacaa agagaggtag
      421 agagagatgg gggtaggttt agatttgtgg gtcatttctc tgaggctcct ggtacatcag
      481 cctttggtac tgctgaacgg cctgaatttg aaagaatatt aaatgagtgt cgggccggtc
      541 gactaaatat gataatagtt tatgatgtat ctaggttcag tagactcaaa gttatggatg
      601 caattccgat cgtgagcgag ctacttgcac tcggtgttac tatcgtaagt acacaagaag
      661 gtgtattcag gcagggtaat gtgatggacc ttatacacct tattatgcga ctcgacgcta
      721 gccataaaga gtcatcactg aagagtgcta aaatcttgga caccaagaac ctgcaaaggg
      781 agcttggagg ctatgtagga ggaaaagctc cgtatggttt cgaactcgtg agtgagacaa
      841 aagagattac cagaaatggt aggatggtga acgtggttat taataagcta gctcatagca
      901 caacccctct cacagggcct tttgagttcg aacctgacgt tattagatgg tggtggagag
      961 agatcaaaac tcacaaacat cttccattta agccaggtag ccaggctgct atccaccctg
     1021 gatctatcac tgggctttgt aaaaggatgg atgctgacgc agtgccaact cgaggggaaa
     1081 caatcggtaa gaagactgca tcttccgctt gggatcccgc tacagttatg agaatccttc
     1141 gggatccgcg tattgctgga ttcgccgcag aagtcattta taagaaaaaa cctgatggta
     1201 ctcctactac aaaaatcgaa ggttatagga tccaaaggga tcctattact ttgcgtccag
     1261 ttgaattaga ctgtggaccg atcatcgaac ctgccgagtg gtacgagttg caggcctggc
     1321 ttgatggtag agggcgtgga aagggtttat ctcggggaca ggcaattttg tcagcaatgg
     1381 ataagcttta ttgtgagtgt ggggctgtta tgacatcaaa acgtggagaa gagtcaatca
     1441 aggattctta caggtgcaga cgtcgtaaag tggtggaccc ttctgctcca ggacagcatg
     1501 agggtacatg caacgtttct atggctgctc ttgataaatt tgtggccgaa cgtattttca
     1561 acaagattag acacgctgaa ggggatgagg aaactttagc tcttctttgg gaggctgccc
     1621 ggcgttttgg taagctaaca gaggcaccag aaaaaagtgg cgaaagagct aatttggtag
     1681 ccgaaagagc agacgccttg aatgcattgg aagaacttta cgaagatagg gctgccggag
     1741 catatgatgg accagttggt cggaagcatt tcaggaagca acaagctgca ctaactttga
     1801 gacagcaagg cgcagaggag cgactcgcag agcttgaggc cgccgaggcc ccaaagcttc
     1861 cactcgatca atggtttcct gaggatgccg acgctgatcc tacaggtcca aagagctggt
     1921 ggggaagagc tagcgtagac gataagagag tgtttgtggg tctatttgtt gacaagattg
     1981 tagttactaa gtccactacg ggtcgaggac agggcactcc catcgaaaag agagccagta
     2041 ttacctgggc aaagcctcca accgatgatg atgaagacga tgcacaagat ggtaccgagg
     2101 atgtggcagc accaaaaaaa aagaggaagg tgtaagcttg gaatggatct tcgatcccga
     2161 tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgac
     2221 gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat
     2281 gacgttattt atgagatggg tttttatgat tagagtcccg caattataca tttaatacgc
     2341 gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcdcgg tgtcatctat
     2401 gttactagat cgggaattgc caagctaatt cttgaagacg aaagggcctc gtgatacgcc
     2461 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     2521 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     2581 cgctcatgag acaataaccc tgataaatgc ttcaataatg ggaccgactc gcgctgtcat
     2641 gagacgaatt ctgacaggat atattggcgg gtaaacctaa gagaaaagag cgtttattag
     2701 aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgca
     2761 tgccaaccac agggttcccc tcgggatcaa agtactttga tccaacccct ccgctgctat
     2821 agtgcagtcg gcttctgacg ttcagtgcag ccgtcatctg aaaacgacat gtcgcacaag
     2881 tcctaagtta cgcgacaggc tgccgccctg cccttttcct ggcgttttct tgtcgcgtgt
     2941 tttagtcgca taaagtagaa tacttgcgac tagaaccgga gacattacgc catgaacaag
     3001 agcgccgccg ctggcctgct gggctatgcc cgcgtcagca ccgacgacca ggacttgacc
     3061 aaccaacggg ccgaactgca cgcggccggc tgcaccaagc tgttttccga gaagatcacc
     3121 ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg accacctacg ccctggcgac
     3181 gttgtgacag tgaccaggct agaccgcctg gcccgcagca cccgcgacct actggacatt
     3241 gccgagcgca tccaggaggc cggcgcgggc ctgcgtagcc tggcagagcc gtgggccgac
     3301 accaccacgc cggccggccg catggtgttg accgtgttcg ccggcattgc cgagttcgag
     3361 cgttccctaa tcatcgaccg cacccggagc gggcgcgagg ccgccaaggc ccgaggcgtg
     3421 aagtttggcc cccgccctac cctcaccccg gcacagatcg cgcacgcccg cgagctgatc
     3481 gaccaggaag gccgcaccgt gaaagaggcg gctgcactgc ttggcgtgca tcgctcgacc
     3541 ctgtaccgcg cacttgagcg cagcgaggaa gtgacgccca ccgaggccag gcggcgcggt
     3601 gccttccgtg aggacgcatt gaccgaggcc gacgccctgg cggccgccga gaatgaacgc
     3661 caagaggaac aagcatgaaa ccgcaccagg acggccagga cgaaccgttt ttcattaccg
     3721 aagagatcga ggcggagatg atcgcggccg ggtacgtgtt cgagccgccc gcgcacctct
     3781 caaccgtgcg gctgcatgaa atcctggccg gtttgtctga tgccaagctg gcggcctggc
     3841 cggccagctt ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg
     3901 agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat ccgatgagta aataaacaaa
     3961 tacgcaaggg gaacgcatga aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa
     4021 gacgaccatc ggaacccatc tagcccgcgc cctgcaactc gccggggccg atgttctgtt
     4081 agtcgattcc gatccccagg gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc
     4141 gctaaccgtt gtcggcatcg accgcccgac gattgaccgc gacgtgaagg ccatcggccg
     4201 gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg gacttggctg tgtccgcgat
     4261 caaggcagcc gacttcgtgc tgattccggt gcagccaagc ccttacgaca tatgggccac
     4321 cgccgacctg gtggagctgg ttaagcagcg cattgaggtc acggatggaa ggctacaagc
     4381 ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc
     4441 gctggccggg tacgagctgc ccattcttga gtcccgtatc acgcagcgcg tgagctaccc
     4501 aggcactgcc gccgccggca caaccgttct tgaatcagaa cccgagggcg acgctgcccg
     4561 cgaggtccag gcgctggccg ctgaaattaa atcaaaactc atttgagtta atgaggtaaa
     4621 gagaaaatga gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc acgcagcagc
     4681 aaggctgcaa cgttggccag cctggcagac acgccagcca tgaagcgggt caactttcag
     4741 ttgccggcgg aggatcacac caagctgaag atgtacgcgg tacgccaagg caagaccatt
     4801 accgagctgc tatctgaata gatcgcgcag ctaccagagt aaatgagcaa atgaataaat
     4861 gagtagatga attttagcgg ctaaaggagg cggcatggaa aatcaagaac aaccaggcac
     4921 cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt tggccaggcg taagcggctg
     4981 ggttgtctgc cggccctgca atggcactgg aacccccaag cccgaggaat cggcgtgacg
     5041 gtcgcaaacc atccggcccg gtacaaatcg gcgcggcgct gggtgatgac ctggtggaga
     5101 agttgaaggc cgcgcaggcc gcccagcggc aacgcatcga ggcagaagca cgccccggtg
     5161 aatcgtggca agcggccgct gatcgaatcc gcaaagaatc ccggcaaccg ccggcagccg
     5221 gtgcgccgtc gattaggaag ccgcccaagg gcgacgagca accagatttt ttcgttccga
     5281 tgctctatga cgtgggcacc cgcgatagtc gcagcatcat ggacgtggcc gttttccgtc
     5341 tgtcgaagcg tgaccgacga gctggcgagg tgatccgcta cgagcttcca gacgggcacg
     5401 tagaggtttc cgcagggccg gccggcatgg ccagtgtgtg ggattacgac ctggtactga
     5461 tggcggtttc ccatctaacc gaatccatga accgataccg ggaagggaag ggagacaagc
     5521 ccggccgcgt gttccgtcca cacgttgcgg acgtactcaa gttctgccgg cgagccgatg
     5581 gcggaaagca gaaagacgac ctggtagaaa cctgcattcg gttaaacacc acgcacgttg
     5641 ccatgcagcg tacgaagaag gccaagaacg gccgcctggt gacggtatcc gagggtgaag
     5701 ccttgattag ccgctacaag atcgtaaaga gcgaaaccgg gcggccggag tacatcgaga
     5761 tcgagctagc tgattggatg taccgcgaga tcacagaagg caagaacccg gacgtgctga
     5821 cggttcaccc cgattacttt ttgatcgatc ccggcatcgg ccgttttctc taccgcctgg
     5881 cacgccgcgc cgcaggcaag gcagaagcca gatggttgtt caagacgatc tacgaacgca
     5941 gtggcagcgc cggagagttc aagaagttct gtttcaccgt gcgcaagctg atcgggtcaa
     6001 atgacctgcc ggagtacgat ttgaaggagg aggcggggca ggctggcccg atcctagtca
     6061 tgcgctaccg caacctgatc gagggcgaag catccgccgg ttcctaatgt acggagcaga
     6121 tgctagggca aattgcccta gcaggggaaa aaggtcgaaa aggactcttt cctgtggata
     6181 gcacgtacat tgggaaccca aagccgtaca ttgggaaccg gaacccgtac attgggaacc
     6241 caaagccgta cattgggaac cggtcacaca tgtaagtgac tgatataaaa gagaaaaaag
     6301 gcgatttttc cgcctaaaac tctttaaaac ttattaaaac tcttaaaacc cgcctggcct
     6361 gtgcataact gtctggccag cgcacagccg aagagctgca aaaagcgcct acccttcggt
     6421 cgctgcgctc cctacgcccc gccgcttcgc gtcggcctat cgcggccgct ggccgctcaa
     6481 aaatggctgg cctacggcca ggcaatctac cagggcgcgg acaagccgcg ccgtcgccac
     6541 tcgaccgccg gcgcccacat caaggcaccc tgcctcgcgc gtttcggtga tgacggtgaa
     6601 aacctctgac acatgcagct cccggtgacg gtcacagctt gtctgtaagc ggatgccggg
     6661 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg
     6721 acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga
     6781 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     6841 accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     6901 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     6961 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     7021 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     7081 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     7141 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     7201 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     7261 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     7321 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     7381 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     7441 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     7501 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     7561 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     7621 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     7681 gttaagggat tttggtcatg cattctaggt gattagaaaa actcatcgag catcaaatga
     7741 aactgcaatt tattcatatc aggattatca ataccatatt tttgaaaaag ccgtttctgt
     7801 aatgaaggag aaaactcacc gaggcagttc cataggatgg caagatcctg gtatcggtct
     7861 gcgattccga ctcgtccaac atcaatacaa cctattaatt tcccctcgtc aaaaataagg
     7921 ttatcaagtg agaaatcacc atgagtgacg actgaatccg gtgagaatgg caaaagttta
     7981 tgcatttctt tccagacttg ttcaacaggc cagccattac gctcgtcatc aaaatcactc
     8041 gcatcaacca aaccgttatt cattcgtgat tgcgcctgag cgagtcgaaa tacgcgatcg
     8101 ctgttaaaag gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc
     8161 gcatcaacaa tattttcacc tgaatcagga tattcttcta atacctggaa tgctgttttc
     8221 cctgggatcg cagtggtgag taaccatgca tcatcaggag tacggataaa atgcttgatg
     8281 gtcggaagag gcataaattc cgtcagccag tttagtctga ccatctcatc tgtaacatca
     8341 ttggcaacgc tacctttgcc atgtttcaga aacaactctg gcgcatcggg cttcccatac
     8401 aatcggtaga ttgtcgcacc tgattgcccg acattatcgc gagcccattt atacccatat
     8461 aaatcagcat ccatgttgga atttaatcgc ggccttgagc aagacgtttc ccgttgaata
     8521 tggctcataa cagaacttat tatttccttc ctcttttcta cagtatttaa agatacccca
     8581 agaagctaat tataacaaga cgaactccaa ttcactgttc cttgcattct aaaaccttaa
     8641 ataccagaaa acagcttttt caaagttgtt ttcaaagttg gcgtataaca tagtatcgac
     8701 ggagccgatt ttgaaaccgc ggtgatcaca ggcagcaacg ctctgtcatc gttacaatca
     8761 acatgctacc ctccgcgaga tcatccgtgt ttcaaacccg gcagcttagt tgccgttctt
     8821 ccgaatagca tcggtaacat gagcaaagtc tgccgcctta caacggctct cccgctgacg
     8881 ccgtcccgga ctgatgggct gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg
     8941 gggagctgtt ggctggctgg tggcaggata tattgtggtg taaacataac gaattcgtct
     9001 ca
//
