LOCUS       sequence-31098-e         921 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Nishino J, Kim I, Chada K, Morrison SJ
  TITLE     Hmga2 promotes neural stem cell self-renewal in young but not old 
            mice by reducing p16Ink4a and p19Arf Expression.
  JOURNAL   Cell. 2008 Oct 17. 135(2):227-39.
  PUBMED    18957199
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2008 
            Oct 17. 135(2):227-39."
COMMENT     Sequence Label: HMGA2 shRNA
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     208..229
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      271..280
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(319..522)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(348..368)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        523..826
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gccttgggca ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg tagcggctga
       61 agcactgcac gccgtaggtc agggtggtca cgagggtggg ccagggcacg ggcagcttgc
      121 cggtggtgca gatgaacttc agggtcagct tgccgtaggt ggcatcgccc tcgccctcgc
      181 cggacacgct gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg atgggcacca
      241 ccccggtgaa cagctcctcg cccttgctca ccatggtggc gaccggtagc gctagcggat
      301 ctgacggttc actaaaccag ctctgcttat atagacctcc caccgtacac gcctaccgcc
      361 catttgcgtc aatggggcgg agttgttacg acattttgga aagtcccgtt gattttggtg
      421 ccaaaacaaa ctcccattga cgtcaatggg gtggagactt ggaaatcccc gtgagtcaaa
      481 ccgctatcca cgcccattga tgtactgcca aaaccgcatc accatggtaa tagcgatgac
      541 taatacgtag atgtactgcc aagtaggaaa gtcccataag gtcatgtact gggcataatg
      601 ccaggcgggc catttaccgt cattgacgtc aatagggggc gtacttggca tatgatacac
      661 ttgatgtact gccaagtggg cagtttaccg taaatactcc acccattgac gtcaatggaa
      721 agtccctatt ggcgttacta tgggaacata cgtcattatt gacgtcaatg ggcgggggtc
      781 gttgggcggt cagccaggcg ggccatttac cgtaagttat gtaacgcgga actccatata
      841 tgggctatga actaatgacc ccgtaattga ttactattaa taactaatgc atggcggtaa
      901 tacgtctaga gcgggccgta t
//
