LOCUS       sequence-310980-       16037 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module for estradiol-inducible expression of the PhiC31 integrase 
            gene and dexamethasone -inducible expression of PhiC31 phage 
            recombination directionality factor (RDF) gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16037)
  AUTHORS   Bernabe-Orts JM, Quijano-Rubio A, Vazquez-Vilar M, Mancheno-Bonillo 
            J, Moles-Casas V, Selma S, Gianoglio S, Granell A, Orzaez D
  TITLE     A memory switch for plant synthetic biology based on the phage 
            varphiC31 integration system.
  JOURNAL   Nucleic Acids Res. 2020 Apr 6;48(6):3379-3394. doi: 
            10.1093/nar/gkaa104.
  PUBMED    32083668
REFERENCE   2  (bases 1 to 16037)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2020-04-6- 6"; volume: "48"; issue: "6";
            pages: "3379-3394"
COMMENT     Sequence Label: pDGB3_alpha2:
            Pnos:ER:lexABD:GAL4AD:T35s-Oplex...
FEATURES             Location/Qualifiers
     source          1..16037
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        83..262
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             304..1239
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPGKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             352..1242
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPGKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATV"
     CDS             1243..1494
                     /codon_start=1
                     /gene="lexA (truncated)"
                     /product="DNA-binding domain of E. coli LexA"
                     /label=LexA BD
                     /note="binds the 16-bp palindromic sequence 
                     5'-CTGTATATATATACAG-3'"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAA"
     terminator      1812..2064
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        2576..2622
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             2697..4511
                     /codon_start=1
                     /product="integrase from phage phi-C31"
                     /label=phage phi-C31 integrase
                     /translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG
                     GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP
                     IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
                     GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
                     IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
                     RDPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
                     WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
                     QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
                     NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
                     APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
                     EKRASITWAKPPTDDDEDDAQDGTEDVAA"
     CDS             4512..4532
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      4559..4811
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        5122..5301
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     primer_bind     complement(6196..6215)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     terminator      7448..7700
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    7960..7979
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    8012..8031
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    8064..8083
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    8116..8135
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    8168..8187
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    8220..8239
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        8313..8359
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     terminator      9192..9444
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    9682..9706
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             11007..11636
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIGTHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             12070..13137
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      13203..13397
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(13633..13655)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    13741..13881
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     13796..13815
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(13896..13913)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(14067..14655)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14147..14166)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(14743..15558)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(14743..15558)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     15466..15485
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    15991..16015
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ggagatacat gagaattaag ggagtcacgg tatgaccccc gccgatgacg cgggacaagc
       61 cgttttacgt ttggaactga cagaaccgca acgttgaagg agccactgag ccgcgggttt
      121 ctggagttta atgagctaag cacatacgtc agaaaccatt attgcgcgtt caaaagtcgc
      181 ctaaggtcac tatcagctag caaatatttc ttgtcaaaaa tgctccactg acgttccata
      241 aattcccctc ggtatccaat tagagtctca tattcactct caactcgatc gaggcccatg
      301 tctgctggag acatgagagc tgccaacctt tggccaagcc cgctcatgat caaacgctct
      361 aagaagaaca gcctggcctt gtccctgacg gccgaccaga tggtcagtgc cttgttggat
      421 gctgagcccc ccatactcta ttccgagtat gatcctacca gacccttcag tgaagcttcg
      481 atgatgggct tactgaccaa cctggcagac agggagctgg ttcacatgat caactgggcg
      541 aagagggtgc caggctttgt ggatttgacc ctccatgatc aggtccacct tctagaatgt
      601 gcctggctag agatcctgat gattggactc gtctggcgct ccatggagca cccagggaag
      661 ctactgtttg ctcctaactt gctcttggac aggaaccagg gaaaatgtgt agagggcatg
      721 gtggagatct tcgacatgct gctggctaca tcatctcggt tccgcatgat gaatctgcag
      781 ggagaggagt ttgtgtgcct caaatctatt attttgctta attctggagt gtacacattt
      841 ctgtccagca ccctgaagtc tctggaagag aaggaccata tccaccgagt cctggacaag
      901 atcacagaca ctttgatcca cctgatggcc aaggcaggcc tgaccctgca gcagcagcac
      961 cagcggctgg cccagctcct cctcatcctc tcccacatca ggcacatgag taacaaaggc
     1021 atggagcatc tgtacagcat gaagtgcaag aacgtggtgc ccctctatga cctgctgctg
     1081 gagatgctgg acgcccaccg cctacatgcg cccactagcc gtggaggggc atccgtggag
     1141 gaaacggacc aaagccactt ggccactgcg ggctctactt cgtcgcattc cttgcaaaag
     1201 tattacatca cgggggaggc agagggtttc cctgccacag taatgaaagc gttaacggcc
     1261 aggcaacaag aggtgtttga tctcatccgt gatcacatca gccagacagg tatgccgccg
     1321 acgcgtgcgg aaatcgcgca gcgtttgggg ttccgttccc caaacgcggc tgaagaacat
     1381 ctgaaggcgc tggcacgcaa aggcgttatt gaaattgttt ccggcgcatc acgcgggatt
     1441 cgtctgttgc aggaagagga agaagggttg ccgctggtag gtcgtgtggc tgccggtgaa
     1501 ccggcaggtg gcagtggagg gggcggaagc gggggtacaa attctcaagc gctttcacaa
     1561 ccaattgcct cctctaacgt tcatgataac ttcatgaata atgaaatcac ggctagtaaa
     1621 attgatgatg gtaataattc aaaaccactg tcacctggtt ggacggacca aactgcgtat
     1681 aacgcgtttg gaatcactac agggatgttt aataccacta caatggatga tgtatataac
     1741 tatctattcg atgatgaaga taccccacca aacccaaaaa aagagtgagc ttggaatgga
     1801 tcttcgatcc cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg
     1861 ccggtcttgc gacgattatc atataatttc tgttgaatta cgttaagcat gtaataatta
     1921 acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat
     1981 acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcd
     2041 cggtgtcatc tatgttacta gatcgggaat tgccaagcta attcttgaag acgaaagggc
     2101 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     2161 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     2221 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atgggaccga
     2281 ctcgcgctgt caggagagct tgggctgcag gtcgaggcta aaaaactaat cgcattatca
     2341 tcccctcgac gtactgtaca tataaccact ggttttatat acagcagtac tgtacatata
     2401 accactggtt ttatatacag cagtcgacgt actgtacata taaccactgg ttttatatac
     2461 agcagtactg tacatataac cactggtttt atatacagca gtcgaggtaa gattagatat
     2521 ggatatgtat atggatatgt atatggtggt aatgccatgt aatatgctcc ccttcgcaag
     2581 acccttcctc tatataagga agttcatttc atttggagag gactccggta tttttacaac
     2641 aattaccaca acaaaacaaa caacaaacaa cattacaatt tactattcta gtcgaaatgg
     2701 acacgtatgc cggcgcttat gatagacaat caagagaaag agagaactct tctgccgcct
     2761 ctccagctac acagagatct gccaacgagg ataaagctgc tgatttacaa agagaggtag
     2821 agagagatgg gggtaggttt agatttgtgg gtcatttctc tgaggctcct ggtacatcag
     2881 cctttggtac tgctgaacgg cctgaatttg aaagaatatt aaatgagtgt cgggccggtc
     2941 gactaaatat gataatagtt tatgatgtat ctaggttcag tagactcaaa gttatggatg
     3001 caattccgat cgtgagcgag ctacttgcac tcggtgttac tatcgtaagt acacaagaag
     3061 gtgtattcag gcagggtaat gtgatggacc ttatacacct tattatgcga ctcgacgcta
     3121 gccataaaga gtcatcactg aagagtgcta aaatcttgga caccaagaac ctgcaaaggg
     3181 agcttggagg ctatgtagga ggaaaagctc cgtatggttt cgaactcgtg agtgagacaa
     3241 aagagattac cagaaatggt aggatggtga acgtggttat taataagcta gctcatagca
     3301 caacccctct cacagggcct tttgagttcg aacctgacgt tattagatgg tggtggagag
     3361 agatcaaaac tcacaaacat cttccattta agccaggtag ccaggctgct atccaccctg
     3421 gatctatcac tgggctttgt aaaaggatgg atgctgacgc agtgccaact cgaggggaaa
     3481 caatcggtaa gaagactgca tcttccgctt gggatcccgc tacagttatg agaatccttc
     3541 gggatccgcg tattgctgga ttcgccgcag aagtcattta taagaaaaaa cctgatggta
     3601 ctcctactac aaaaatcgaa ggttatagga tccaaaggga tcctattact ttgcgtccag
     3661 ttgaattaga ctgtggaccg atcatcgaac ctgccgagtg gtacgagttg caggcctggc
     3721 ttgatggtag agggcgtgga aagggtttat ctcggggaca ggcaattttg tcagcaatgg
     3781 ataagcttta ttgtgagtgt ggggctgtta tgacatcaaa acgtggagaa gagtcaatca
     3841 aggattctta caggtgcaga cgtcgtaaag tggtggaccc ttctgctcca ggacagcatg
     3901 agggtacatg caacgtttct atggctgctc ttgataaatt tgtggccgaa cgtattttca
     3961 acaagattag acacgctgaa ggggatgagg aaactttagc tcttctttgg gaggctgccc
     4021 ggcgttttgg taagctaaca gaggcaccag aaaaaagtgg cgaaagagct aatttggtag
     4081 ccgaaagagc agacgccttg aatgcattgg aagaacttta cgaagatagg gctgccggag
     4141 catatgatgg accagttggt cggaagcatt tcaggaagca acaagctgca ctaactttga
     4201 gacagcaagg cgcagaggag cgactcgcag agcttgaggc cgccgaggcc ccaaagcttc
     4261 cactcgatca atggtttcct gaggatgccg acgctgatcc tacaggtcca aagagctggt
     4321 ggggaagagc tagcgtagac gataagagag tgtttgtggg tctatttgtt gacaagattg
     4381 tagttactaa gtccactacg ggtcgaggac agggcactcc catcgaaaag agagccagta
     4441 ttacctgggc aaagcctcca accgatgatg atgaagacga tgcacaagat ggtaccgagg
     4501 atgtggcagc accaaaaaaa aagaggaagg tgtaagcttg gaatggatct tcgatcccga
     4561 tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgac
     4621 gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat
     4681 gacgttattt atgagatggg tttttatgat tagagtcccg caattataca tttaatacgc
     4741 gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcdcgg tgtcatctat
     4801 gttactagat cgggaattgc caagctaatt cttgaagacg aaagggcctc gtgatacgcc
     4861 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     4921 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     4981 cgctcatgag acaataaccc tgataaatgc ttcaataatg ggaccgactc gcgctgtcag
     5041 gagatacatg agaattaagg gagtcacggt atgacccccg ccgatgacgc gggacaagcc
     5101 gttttacgtt tggaactgac agaaccgcaa cgttgaagga gccactgagc cgcgggtttc
     5161 tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc aaaagtcgcc
     5221 taaggtcact atcagctagc aaatatttct tgtcaaaaat gctccactga cgttccataa
     5281 attcccctcg gtatccaatt agagtctcat attcactctc aactcgatcg aggcccatga
     5341 ttcagcaagc cactgcagga gtctcacaag acacttcgga aaatcctaac aaaacaatag
     5401 ttcctgcagc attaccacag ctcaccccta ccttggtgtc actgctggag gtgattgaac
     5461 ccgaggtgtt gtatgcagga tatgatagct ctgttccaga ttcagcatgg agaattatga
     5521 ccacactcaa catgttaggt gggcgtcaag tgattgcagc agtgaaatgg gcaaaggcga
     5581 taccaggctt cagaaactta cacctggatg accaaatgac cctgctacag tactcatgga
     5641 tgtttctcat ggcatttgcc ctgggttgga gatcatacag acaatcaagt ggaaacctgc
     5701 tctgctttgc tcctgatctg attattaatg agcagagaat gtctctaccc tgcatgtatg
     5761 accaatgtaa acacatgctg tttgtctcct ctgaattaca aagattgcag gtatcctatg
     5821 aagagtatct ctgtatgaaa accttactgc ttctctcctc agttcctaag gaaggtctga
     5881 agagccaaga gttatttgat gagattcgaa tgacttatat caaagagcta ggaaaagcca
     5941 tcgtcaaaag ggaagggaac tccagtcaga actggcaacg gttttaccaa ctgacaaagc
     6001 ttctggactc catgcatgag gtggttgaga atctccttac ctactgcttc cagacatttt
     6061 tggataagac catgagtatt gaattcccag agatgttagc tgaaatcatc actaatcaga
     6121 taccaaaata ttcaaatgga aatatcaaaa agcttctgtt tcatcaaaaa tcaatggtga
     6181 aaccagtaac gttatacgat gtcgcagagt atgccggtgt ctcttatcag accgtttccc
     6241 gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcaa
     6301 tggcggagct gaattacatt cccaaccgcg tggcacaaca actggcgggc aaacagtcgt
     6361 tgctgattgg cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg
     6421 cgattaaatc tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa
     6481 gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc
     6541 tgatcattaa ctatccgctg gatgaccagg atgccattgc tgtggaagct gcctgcacta
     6601 atgttccggc gttatttctt gatgtctctg accagacacc catcaacagt attattttct
     6661 cccatgaaga cggtacgcga ctgggcgtgg agcatctggt cgcattgggt caccagcaaa
     6721 tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg tctgcgtctg gctggctggc
     6781 ataaatatct cactcgcaat caaattcagc cgatagcgga acgggaaggc gactggagtg
     6841 ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt cccactgcta
     6901 tgctggttgc caacgatcag atggcgctgg gcgcaatgcg cgccattacc gagtccgggc
     6961 tgcgcgttgg tgcggatatc tcggtagtgg gatacgacga taccgaagac agctcatgtt
     7021 atatcccgcc gttaaccacc atcaaacagg attttcgcct gctggggcaa accagcgtgg
     7081 accgcttgct gcaactctct cagggccagg cggtgaaggg caatcagctg ttgcccgtat
     7141 cactggtgaa aagaaaaacc accctggagc caacaaattc tcaagcgctt tcacaaccaa
     7201 ttgcctcctc taacgttcat gataacttca tgaataatga aatcacggct agtaaaattg
     7261 atgatggtaa taattcaaaa ccactgtcac ctggttggac ggaccaaact gcgtataacg
     7321 cgtttggaat cactacaggg atgtttaata ccactacaat ggatgatgta tataactatc
     7381 tattcgatga tgaagatacc ccaccaaacc caaaaaaaga gtgagcttgg aatggatctt
     7441 cgatcccgat cgttcaaaca tttggcaata aagtttctta agattgaatc ctgttgccgg
     7501 tcttgcgacg attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat
     7561 gtaatgcatg acgttattta tgagatgggt ttttatgatt agagtcccgc aattatacat
     7621 ttaatacgcg atagaaaaca aaatatagcg cgcaaactag gataaattat cgcgcdcggt
     7681 gtcatctatg ttactagatc gggaattgcc aagctaattc ttgaagacga aagggcctcg
     7741 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     7801 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     7861 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatgg gaccgactcg
     7921 cgctgtcagg agttgcgaag atcgatccac tagtctttca attgtgagcg ctcacaattc
     7981 tttctcttcc ctttcttctt tctagtcttt caattgtgag cgctcacaat tctttctctt
     8041 ccctttcttc tttctagtct ttcaattgtg agcgctcaca attctttctc ttccctttct
     8101 tctttctagt ctttcaattg tgagcgctca caattctttc tcttcccttt cttctttcta
     8161 gtctttcaat tgtgagcgct cacaattctt tctcttccct ttcttctttc ttgtctttca
     8221 attgtgagcg ctcacaattc tttctcttcc ctttcttctt tctagctcca ccgcggtggc
     8281 ggccggccgc tctagtggat cgattcccct tcgcaagacc cttcctctat ataaggaagt
     8341 tcatttcatt tggagaggac tccggtattt ttacaacaat taccacaaca aaacaaacaa
     8401 caaacaacat tacaatttac tattctagtc gaaatggcta agagatctat ttgggctggt
     8461 gatgaagata ataagcctaa gaagagagaa acttatgctg atgatactgt tggtagattt
     8521 cattctggtt attctgaaac taatgaaaga ggtaaggttg ttcctgttgc tcttgataag
     8581 tggagaattt ctactggtga acaatctgtt gctgatgctg ttgctcaact ttttggtggt
     8641 actcctgttg aaaatgaaga atctacttct gaaaatttta ttgatgtttt tactgataga
     8701 cctaaggttc ctgttattat tgaagctgat ggtattcatt gggatatgaa gctttggctt
     8761 aatggtaagc ttaagcatca ttgtgatggt tttgattttg tttctcatgc tgatgaagaa
     8821 atgattggtc aaccttgtgg ttgtcctaag ctttttgatg aaagaaaggc tgctgctaag
     8881 gaatatgatg ctcctaatcc tgctattact gttactttta ctcttgctga tgatcctgaa
     8941 cttggtagat ttaagtttca aactggttct tggactcttt ttaaggttct tcatgaagct
     9001 gaagatgatg ttgaaagagt tggtaagggt ggtgctgttc ttgctaatct tgaacttgaa
     9061 cttgttgaat atactcctaa gagaggtcct atgagaaata agcttgtttc ttattataag
     9121 cctactatta ctgttcttaa gtcttataat gatgctattg ctgattaggc ttggaatgga
     9181 tcttcgatcc cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg
     9241 ccggtcttgc gacgattatc atataatttc tgttgaatta cgttaagcat gtaataatta
     9301 acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat
     9361 acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcd
     9421 cggtgtcatc tatgttacta gatcgggaat tgccaagcta attcttgaag acgaaagggc
     9481 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     9541 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     9601 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atgggaccga
     9661 ctcgcgcttg agacgaagct ttgacaggat atattggcgg gtaaacctaa gagaaaagag
     9721 cgtttattag aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt
     9781 tgtatgtgca tgccaaccac agggttcccc tcgggatcaa agtactttga tccaacccct
     9841 ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag ccgtcatctg aaaacgacat
     9901 gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg cccttttcct ggcgttttct
     9961 tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac tagaaccgga gacattacgc
    10021 catgaacaag agcgccgccg ctggcctgct gggctatgcc cgcgtcagca ccgacgacca
    10081 ggacttgacc aaccaacggg ccgaactgca cgcggccggc tgcaccaagc tgttttccga
    10141 gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg accacctacg
    10201 ccctggcgac gttgtgacag tgaccaggct agaccgcctg gcccgcagca cccgcgacct
    10261 actggacatt gccgagcgca tccaggaggc cggcgcgggc ctgcgtagcc tggcagagcc
    10321 gtgggccgac accaccacgc cggccggccg catggtgttg accgtgttcg ccggcattgc
    10381 cgagttcgag cgttccctaa tcatcgaccg cacccggagc gggcgcgagg ccgccaaggc
    10441 ccgaggcgtg aagtttggcc cccgccctac cctcaccccg gcacagatcg cgcacgcccg
    10501 cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg gctgcactgc ttggcgtgca
    10561 tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa gtgacgccca ccgaggccag
    10621 gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc gacgccctgg cggccgccga
    10681 gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg acggccagga cgaaccgttt
    10741 ttcattaccg aagagatcga ggcggagatg atcgcggccg ggtacgtgtt cgagccgccc
    10801 gcgcacctct caaccgtgcg gctgcatgaa atcctggccg gtttgtctga tgccaagctg
    10861 gcggcctggc cggccagctt ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga
    10921 tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat ccgatgagta
    10981 aataaacaaa tacgcaaggg gaacgcatga aggttatcgc tgtacttaac cagaaaggcg
    11041 ggtcaggcaa gacgaccatc ggaacccatc tagcccgcgc cctgcaactc gccggggccg
    11101 atgttctgtt agtcgattcc gatccccagg gcagtgcccg cgattgggcg gccgtgcggg
    11161 aagatcaacc gctaaccgtt gtcggcatcg accgcccgac gattgaccgc gacgtgaagg
    11221 ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg gacttggctg
    11281 tgtccgcgat caaggcagcc gacttcgtgc tgattccggt gcagccaagc ccttacgaca
    11341 tatgggccac cgccgacctg gtggagctgg ttaagcagcg cattgaggtc acggatggaa
    11401 ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg
    11461 ttgccgaggc gctggccggg tacgagctgc ccattcttga gtcccgtatc acgcagcgcg
    11521 tgagctaccc aggcactgcc gccgccggca caaccgttct tgaatcagaa cccgagggcg
    11581 acgctgcccg cgaggtccag gcgctggccg ctgaaattaa atcaaaactc atttgagtta
    11641 atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc
    11701 acgcagcagc aaggctgcaa cgttggccag cctggcagac acgccagcca tgaagcgggt
    11761 caactttcag ttgccggcgg aggatcacac caagctgaag atgtacgcgg tacgccaagg
    11821 caagaccatt accgagctgc tatctgaata gatcgcgcag ctaccagagt aaatgagcaa
    11881 atgaataaat gagtagatga attttagcgg ctaaaggagg cggcatggaa aatcaagaac
    11941 aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt tggccaggcg
    12001 taagcggctg ggttgtctgc cggccctgca atggcactgg aacccccaag cccgaggaat
    12061 cggcgtgacg gtcgcaaacc atccggcccg gtacaaatcg gcgcggcgct gggtgatgac
    12121 ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc aacgcatcga ggcagaagca
    12181 cgccccggtg aatcgtggca agcggccgct gatcgaatcc gcaaagaatc ccggcaaccg
    12241 ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg gcgacgagca accagatttt
    12301 ttcgttccga tgctctatga cgtgggcacc cgcgatagtc gcagcatcat ggacgtggcc
    12361 gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg tgatccgcta cgagcttcca
    12421 gacgggcacg tagaggtttc cgcagggccg gccggcatgg ccagtgtgtg ggattacgac
    12481 ctggtactga tggcggtttc ccatctaacc gaatccatga accgataccg ggaagggaag
    12541 ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg acgtactcaa gttctgccgg
    12601 cgagccgatg gcggaaagca gaaagacgac ctggtagaaa cctgcattcg gttaaacacc
    12661 acgcacgttg ccatgcagcg tacgaagaag gccaagaacg gccgcctggt gacggtatcc
    12721 gagggtgaag ccttgattag ccgctacaag atcgtaaaga gcgaaaccgg gcggccggag
    12781 tacatcgaga tcgagctagc tgattggatg taccgcgaga tcacagaagg caagaacccg
    12841 gacgtgctga cggttcaccc cgattacttt ttgatcgatc ccggcatcgg ccgttttctc
    12901 taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca gatggttgtt caagacgatc
    12961 tacgaacgca gtggcagcgc cggagagttc aagaagttct gtttcaccgt gcgcaagctg
    13021 atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg aggcggggca ggctggcccg
    13081 atcctagtca tgcgctaccg caacctgatc gagggcgaag catccgccgg ttcctaatgt
    13141 acggagcaga tgctagggca aattgcccta gcaggggaaa aaggtcgaaa aggactcttt
    13201 cctgtggata gcacgtacat tgggaaccca aagccgtaca ttgggaaccg gaacccgtac
    13261 attgggaacc caaagccgta cattgggaac cggtcacaca tgtaagtgac tgatataaaa
    13321 gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac ttattaaaac tcttaaaacc
    13381 cgcctggcct gtgcataact gtctggccag cgcacagccg aagagctgca aaaagcgcct
    13441 acccttcggt cgctgcgctc cctacgcccc gccgcttcgc gtcggcctat cgcggccgct
    13501 ggccgctcaa aaatggctgg cctacggcca ggcaatctac cagggcgcgg acaagccgcg
    13561 ccgtcgccac tcgaccgccg gcgcccacat caaggcaccc tgcctcgcgc gtttcggtga
    13621 tgacggtgaa aacctctgac acatgcagct cccggtgacg gtcacagctt gtctgtaagc
    13681 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
    13741 cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca
    13801 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta
    13861 aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
    13921 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
    13981 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
    14041 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
    14101 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
    14161 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
    14221 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
    14281 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
    14341 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
    14401 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
    14461 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
    14521 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
    14581 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
    14641 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
    14701 gaaaactcac gttaagggat tttggtcatg cattctaggt gattagaaaa actcatcgag
    14761 catcaaatga aactgcaatt tattcatatc aggattatca ataccatatt tttgaaaaag
    14821 ccgtttctgt aatgaaggag aaaactcacc gaggcagttc cataggatgg caagatcctg
    14881 gtatcggtct gcgattccga ctcgtccaac atcaatacaa cctattaatt tcccctcgtc
    14941 aaaaataagg ttatcaagtg agaaatcacc atgagtgacg actgaatccg gtgagaatgg
    15001 caaaagttta tgcatttctt tccagacttg ttcaacaggc cagccattac gctcgtcatc
    15061 aaaatcactc gcatcaacca aaccgttatt cattcgtgat tgcgcctgag cgagtcgaaa
    15121 tacgcgatcg ctgttaaaag gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa
    15181 cactgccagc gcatcaacaa tattttcacc tgaatcagga tattcttcta atacctggaa
    15241 tgctgttttc cctgggatcg cagtggtgag taaccatgca tcatcaggag tacggataaa
    15301 atgcttgatg gtcggaagag gcataaattc cgtcagccag tttagtctga ccatctcatc
    15361 tgtaacatca ttggcaacgc tacctttgcc atgtttcaga aacaactctg gcgcatcggg
    15421 cttcccatac aatcggtaga ttgtcgcacc tgattgcccg acattatcgc gagcccattt
    15481 atacccatat aaatcagcat ccatgttgga atttaatcgc ggccttgagc aagacgtttc
    15541 ccgttgaata tggctcataa cagaacttat tatttccttc ctcttttcta cagtatttaa
    15601 agatacccca agaagctaat tataacaaga cgaactccaa ttcactgttc cttgcattct
    15661 aaaaccttaa ataccagaaa acagcttttt caaagttgtt ttcaaagttg gcgtataaca
    15721 tagtatcgac ggagccgatt ttgaaaccgc ggtgatcaca ggcagcaacg ctctgtcatc
    15781 gttacaatca acatgctacc ctccgcgaga tcatccgtgt ttcaaacccg gcagcttagt
    15841 tgccgttctt ccgaatagca tcggtaacat gagcaaagtc tgccgcctta caacggctct
    15901 cccgctgacg ccgtcccgga ctgatgggct gcctgtatcg agtggtgatt ttgtgccgag
    15961 ctgccggtcg gggagctgtt ggctggctgg tggcaggata tattgtggtg taaacataac
    16021 aagcttcgtc tcagtca
//
