LOCUS       sequence-310985-        9518 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TU for the constitutive expression of the MS2 coat protein fused on 
            Ct to the activation domain VPR (VP64+p65+Rta).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9518)
  AUTHORS   Selma S, Bernabe-Orts JM, Vazquez-Vilar M, Diego-Martin B, Ajenjo M,
            Garcia-Carpintero V, Granell A, Orzaez D
  TITLE     Strong gene activation in plants with genome-wide specificity using 
            a new orthogonal CRISPR/Cas9-based programmable transcriptional 
            activator.
  JOURNAL   Plant Biotechnol J. 2019 Sep;17(9):1703-1705. doi: 
            10.1111/pbi.13138. Epub 2019 May 23.
  PUBMED    31034138
REFERENCE   2  (bases 1 to 9518)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/pbi.13138";
            journalName: "Plant Biotechnol J"; date: "2019-09"; volume: "17"; 
            issue: "9"; pages: "1703-1705"
COMMENT     Sequence Label: pDGB3 alpha2 P35S:MS2:VPR:Tnos (GB1830)
FEATURES             Location/Qualifiers
     source          1..9518
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        637..981
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     925..945
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1060..1446
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2
                     /note="binds to a specific stem-loop structure in the viral
                     RNA (Peabody, 1993)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQNRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             1453..1473
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1477..1497
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1501..1521
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1522..1671
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             1672..1692
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1696..1716
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1717..2073
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     CDS             2098..2667
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     terminator      2694..2946
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2981..2999)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    3184..3208
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4509..5138
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIGTHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5572..6639
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      6705..6899
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7135..7157)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7243..7383
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7298..7317
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7398..7415)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7569..8157)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7649..7668)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8245..9060)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(8245..9060)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8968..8987
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    9493..9517
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 taacaagctt cgtctcagtc aggagactag agccaagctg atctcctttg ccccggagat
       61 caccatggac gactttctct atctctacga tctaggaaga aagttcgacg gagaaggtga
      121 cgataccatg ttcaccaccg ataatgagaa gattagcctc ttcaatttca gaaagaatgc
      181 tgacccacag atggttagag aggcctacgc ggcaggtctg atcaagacga tctacccgag
      241 taataatctc caggagatca aataccttcc caagaaggtt aaagatgcag tcaaaagatt
      301 caggactaac tgcatcaaga acacagagaa agatatattt ctcaagatca gaagtactat
      361 tccagtatgg acgattcaag gcttgcttca taaaccaagg caagtaatag agattggagt
      421 ctctaagaaa gtagttccta ctgaatcaaa ggccatggag tcaaaaattc agatcgagga
      481 tctaacagaa ctcgccgtga agactggcga acagttcata cagagtcttt tacgactcaa
      541 tgacaagaag aaaatcttcg tcaacatggt ggagcacgac actctcgtct actccaagaa
      601 tatcaaagat acagtctcag aagaccaaag ggctattgag acttttcaac aaagggtaat
      661 atcgggaaac ctcctcggat tccattgccc agctatctgt cacttcatca aaaggacagt
      721 agaaaaggaa ggtggcacct acaaatgcca tcattgcgat aaaggaaagg ctatcgttca
      781 agatgcctct gccgacagtg gtcccaaaga tggaccccca cccacgagga gcatcgtgga
      841 aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgata tctccactga
      901 cgtaagggat gacgcacaat cccactatcc ttcgcaagac ccttcctcta tataaggaag
      961 ttcatttcat ttggagagga ctccggtatt tttacaacaa taccacaaca aaacaaacaa
     1021 caaacaacat tacaatttac tattctagtc gaaatgatgg cttctaattt tactcaattt
     1081 gttcttgttg ataatggtgg tactggtgat gttactgttg ctccttctaa ttttgctaat
     1141 ggtgttgctg aatggatttc ttctaattct agatctcaag cttataaggt tacttgttct
     1201 gttagacaat cttctgctca aaatagaaag tatactatta aggttgaagt tcctaaggtt
     1261 gctactcaaa ctgttggtgg tgttgaactt cctgttgctg cttggagatc ttatcttaat
     1321 atggaactta ctattcctat ttttgctact aattctgatt gtgaacttat tgttaaggct
     1381 atgcaaggtc ttcttaagga tggtaatcct attccttctg ctattgctgc taattctggt
     1441 atttattcgg acccaaaaaa gaagcgtaag gtcgatccca agaagaagag aaaggtagat
     1501 cctaagaaga agagaaaggt agacgcattg gatgacttcg acttagacat gcttgggtca
     1561 gatgctttgg acgactttga cctcgacatg ttgggttctg acgcgctaga tgacttcgac
     1621 ttggacatgt tgggctccga cgctttggac gactttgatc tggacatgtt accgaaaaag
     1681 aaacgcaaag ttgatcctaa gaagaagaga aaggtaccta cacaggccgg cgagggcaca
     1741 ctgtctgaag ctctgctgca gctgcagttc gacgacgagg atctgggagc cctgctggga
     1801 aacagcaccg atcctgccgt gttcaccgac ctggccagcg tggacaacag cgagttccag
     1861 cagctgctga accagggcat ccctgtggcc cctcacacca ccgagcccat gctgatggaa
     1921 taccccgagg ccatcacccg gctcgtgaca ggcgctcaga ggcctcctga tccagctcct
     1981 gcccctctgg gagcaccagg cctgcctaat ggactgctgt ctggcgacga ggacttcagc
     2041 tctatcgccg atatggattt ctcagccttg ctgattaact ctagaagttc cggatctcgg
     2101 gattccaggg aagggatgtt tttgccgaag cctgaggccg gctccgctat tagtgacgtg
     2161 tttgagggcc gcgaggtgtg ccagccaaaa cgaatccggc catttcatcc tccaggaagt
     2221 ccatgggcca accgcccact ccccgccagc ctcgcaccaa caccaaccgg tccagtacat
     2281 gagccagtcg ggtcactgac cccggcacca gtccctcagc cactggatcc agcgcccgca
     2341 gtgactcccg aggccagtca cctgttggag gatcccgatg aagaaacgag ccaggctgtc
     2401 aaagcccttc gggagatggc cgatactgtg attccccaga aggaagaggc tgcaatctgt
     2461 ggccaaatgg acctttccca tccgccccca aggggccatc tggatgagct gacaaccaca
     2521 cttgagtcca tgaccgagga tctgaacctg gactcacccc tgaccccgga attgaacgag
     2581 attctggata ccttcctgaa cgacgagtgc ctcttgcatg ccatgcatat cagcacagga
     2641 ctgtccatct tcgacacatc tctgttttga gcttggaatg gatcttcgat cccgatcgtt
     2701 caaacatttg gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgacgatta
     2761 tcatataatt tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt
     2821 tatttatgag atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag
     2881 aaaacaaaat atagcgcgca aactaggata aattatcgcg cdcggtgtca tctatgttac
     2941 tagatcggga attgccaagc taattcttga agacgaaagg gcctcgtgat acgcctattt
     3001 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     3061 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     3121 atgagacaat aaccctgata aatgcttcaa taatgggacc gactcgcgct tgagacgaag
     3181 ctttgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt agaataatcg
     3241 gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc
     3301 acagggttcc cctcgggatc aaagtacttt gatccaaccc ctccgctgct atagtgcagt
     3361 cggcttctga cgttcagtgc agccgtcatc tgaaaacgac atgtcgcaca agtcctaagt
     3421 tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg
     3481 cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca agagcgccgc
     3541 cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga ccaaccaacg
     3601 ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca ccggcaccag
     3661 gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta cgccctggcg acgttgtgac
     3721 agtgaccagg ctagaccgcc tggcccgcag cacccgcgac ctactggaca ttgccgagcg
     3781 catccaggag gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg acaccaccac
     3841 gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg agcgttccct
     3901 aatcatcgac cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg
     3961 cccccgccct accctcaccc cggcacagat cgcgcacgcc cgcgagctga tcgaccagga
     4021 aggccgcacc gtgaaagagg cggctgcact gcttggcgtg catcgctcga ccctgtaccg
     4081 cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg
     4141 tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac gccaagagga
     4201 acaagcatga aaccgcacca ggacggccag gacgaaccgt ttttcattac cgaagagatc
     4261 gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacct ctcaaccgtg
     4321 cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg gccggccagc
     4381 ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac
     4441 agcttgcgtc atgcggtcgc tgcgtatatg atccgatgag taaataaaca aatacgcaag
     4501 gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc aagacgacca
     4561 tcggaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg ttagtcgatt
     4621 ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg
     4681 ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc cggcgcgact
     4741 tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag
     4801 ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc accgccgacc
     4861 tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa gcggcctttg
     4921 tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag gcgctggccg
     4981 ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac ccaggcactg
     5041 ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc
     5101 aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta aagagaaaat
     5161 gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc
     5221 aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc agttgccggc
     5281 ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca ttaccgagct
     5341 gctatctgaa tagatcgcgc agctaccaga gtaaatgagc aaatgaataa atgagtagat
     5401 gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc accgacgccg
     5461 tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc tgggttgtct
     5521 gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga cggtcgcaaa
     5581 ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg acctggtgga gaagttgaag
     5641 gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg tgaatcgtgg
     5701 caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc cggtgcgccg
     5761 tcgattagga agccgcccaa gggcgacgag caaccagatt ttttcgttcc gatgctctat
     5821 gacgtgggca cccgcgatag tcgcagcatc atggacgtgg ccgttttccg tctgtcgaag
     5881 cgtgaccgac gagctggcga ggtgatccgc tacgagcttc cagacgggca cgtagaggtt
     5941 tccgcagggc cggccggcat ggccagtgtg tgggattacg acctggtact gatggcggtt
     6001 tcccatctaa ccgaatccat gaaccgatac cgggaaggga agggagacaa gcccggccgc
     6061 gtgttccgtc cacacgttgc ggacgtactc aagttctgcc ggcgagccga tggcggaaag
     6121 cagaaagacg acctggtaga aacctgcatt cggttaaaca ccacgcacgt tgccatgcag
     6181 cgtacgaaga aggccaagaa cggccgcctg gtgacggtat ccgagggtga agccttgatt
     6241 agccgctaca agatcgtaaa gagcgaaacc gggcggccgg agtacatcga gatcgagcta
     6301 gctgattgga tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct gacggttcac
     6361 cccgattact ttttgatcga tcccggcatc ggccgttttc tctaccgcct ggcacgccgc
     6421 gccgcaggca aggcagaagc cagatggttg ttcaagacga tctacgaacg cagtggcagc
     6481 gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc aaatgacctg
     6541 ccggagtacg atttgaagga ggaggcgggg caggctggcc cgatcctagt catgcgctac
     6601 cgcaacctga tcgagggcga agcatccgcc ggttcctaat gtacggagca gatgctaggg
     6661 caaattgccc tagcagggga aaaaggtcga aaaggactct ttcctgtgga tagcacgtac
     6721 attgggaacc caaagccgta cattgggaac cggaacccgt acattgggaa cccaaagccg
     6781 tacattggga accggtcaca catgtaagtg actgatataa aagagaaaaa aggcgatttt
     6841 tccgcctaaa actctttaaa acttattaaa actcttaaaa cccgcctggc ctgtgcataa
     6901 ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc ctacccttcg gtcgctgcgc
     6961 tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc aaaaatggct
     7021 ggcctacggc caggcaatct accagggcgc ggacaagccg cgccgtcgcc actcgaccgc
     7081 cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt gatgacggtg aaaacctctg
     7141 acacatgcag ctcccggtga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     7201 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc
     7261 acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg
     7321 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
     7381 aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     7441 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     7501 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     7561 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     7621 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     7681 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     7741 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     7801 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     7861 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     7921 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     7981 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     8041 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     8101 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     8161 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     8221 attttggtca tgcattctag gtgattagaa aaactcatcg agcatcaaat gaaactgcaa
     8281 tttattcata tcaggattat caataccata tttttgaaaa agccgtttct gtaatgaagg
     8341 agaaaactca ccgaggcagt tccataggat ggcaagatcc tggtatcggt ctgcgattcc
     8401 gactcgtcca acatcaatac aacctattaa tttcccctcg tcaaaaataa ggttatcaag
     8461 tgagaaatca ccatgagtga cgactgaatc cggtgagaat ggcaaaagtt tatgcatttc
     8521 tttccagact tgttcaacag gccagccatt acgctcgtca tcaaaatcac tcgcatcaac
     8581 caaaccgtta ttcattcgtg attgcgcctg agcgagtcga aatacgcgat cgctgttaaa
     8641 aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac
     8701 aatattttca cctgaatcag gatattcttc taatacctgg aatgctgttt tccctgggat
     8761 cgcagtggtg agtaaccatg catcatcagg agtacggata aaatgcttga tggtcggaag
     8821 aggcataaat tccgtcagcc agtttagtct gaccatctca tctgtaacat cattggcaac
     8881 gctacctttg ccatgtttca gaaacaactc tggcgcatcg ggcttcccat acaatcggta
     8941 gattgtcgca cctgattgcc cgacattatc gcgagcccat ttatacccat ataaatcagc
     9001 atccatgttg gaatttaatc gcggccttga gcaagacgtt tcccgttgaa tatggctcat
     9061 aacagaactt attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta
     9121 attataacaa gacgaactcc aattcactgt tccttgcatt ctaaaacctt aaataccaga
     9181 aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga
     9241 ttttgaaacc gcggtgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta
     9301 ccctccgcga gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag
     9361 catcggtaac atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg
     9421 gactgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg
     9481 ttggctggct ggtggcagga tatattgtgg tgtaaaca
//
