LOCUS       sequence-310988-        9698 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TU for the constitutive expression of Ms2 fused to TV (TALx6 - 
            VP128) activation domains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9698)
  AUTHORS   Selma S, Bernabe-Orts JM, Vazquez-Vilar M, Diego-Martin B, Ajenjo M,
            Garcia-Carpintero V, Granell A, Orzaez D
  TITLE     Strong gene activation in plants with genome-wide specificity using 
            a new orthogonal CRISPR/Cas9-based programmable transcriptional 
            activator.
  JOURNAL   Plant Biotechnol J. 2019 Sep;17(9):1703-1705. doi: 
            10.1111/pbi.13138. Epub 2019 May 23.
  PUBMED    31034138
REFERENCE   2  (bases 1 to 9698)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/pbi.13138";
            journalName: "Plant Biotechnol J"; date: "2019-09"; volume: "17"; 
            issue: "9"; pages: "1703-1705"
COMMENT     Sequence Label: pDGB3 alpha2 P35S:MS2:TV:Tnos (GB2048)
FEATURES             Location/Qualifiers
     source          1..9698
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        637..981
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     925..945
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1060..1446
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2
                     /note="binds to a specific stem-loop structure in the viral
                     RNA (Peabody, 1993)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQNRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             1453..1473
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1477..1497
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2536..2685
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             2698..2847
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     terminator      2874..3126
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3161..3179)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    3364..3388
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4689..5318
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIGTHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5752..6819
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      6885..7079
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7315..7337)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7423..7563
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7478..7497
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7578..7595)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7749..8337)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7829..7848)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8425..9240)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(8425..9240)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     9148..9167
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    9673..9697
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 taacaagctt cgtctcagtc aggagactag agccaagctg atctcctttg ccccggagat
       61 caccatggac gactttctct atctctacga tctaggaaga aagttcgacg gagaaggtga
      121 cgataccatg ttcaccaccg ataatgagaa gattagcctc ttcaatttca gaaagaatgc
      181 tgacccacag atggttagag aggcctacgc ggcaggtctg atcaagacga tctacccgag
      241 taataatctc caggagatca aataccttcc caagaaggtt aaagatgcag tcaaaagatt
      301 caggactaac tgcatcaaga acacagagaa agatatattt ctcaagatca gaagtactat
      361 tccagtatgg acgattcaag gcttgcttca taaaccaagg caagtaatag agattggagt
      421 ctctaagaaa gtagttccta ctgaatcaaa ggccatggag tcaaaaattc agatcgagga
      481 tctaacagaa ctcgccgtga agactggcga acagttcata cagagtcttt tacgactcaa
      541 tgacaagaag aaaatcttcg tcaacatggt ggagcacgac actctcgtct actccaagaa
      601 tatcaaagat acagtctcag aagaccaaag ggctattgag acttttcaac aaagggtaat
      661 atcgggaaac ctcctcggat tccattgccc agctatctgt cacttcatca aaaggacagt
      721 agaaaaggaa ggtggcacct acaaatgcca tcattgcgat aaaggaaagg ctatcgttca
      781 agatgcctct gccgacagtg gtcccaaaga tggaccccca cccacgagga gcatcgtgga
      841 aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgata tctccactga
      901 cgtaagggat gacgcacaat cccactatcc ttcgcaagac ccttcctcta tataaggaag
      961 ttcatttcat ttggagagga ctccggtatt tttacaacaa taccacaaca aaacaaacaa
     1021 caaacaacat tacaatttac tattctagtc gaaatgatgg cttctaattt tactcaattt
     1081 gttcttgttg ataatggtgg tactggtgat gttactgttg ctccttctaa ttttgctaat
     1141 ggtgttgctg aatggatttc ttctaattct agatctcaag cttataaggt tacttgttct
     1201 gttagacaat cttctgctca aaatagaaag tatactatta aggttgaagt tcctaaggtt
     1261 gctactcaaa ctgttggtgg tgttgaactt cctgttgctg cttggagatc ttatcttaat
     1321 atggaactta ctattcctat ttttgctact aattctgatt gtgaacttat tgttaaggct
     1381 atgcaaggtc ttcttaagga tggtaatcct attccttctg ctattgctgc taattctggt
     1441 atttattcgg acccgaagaa aaagaggaag gtagatccta agaagaagag aaaggtactg
     1501 ttggatccgg gaacacctat ggacgctgat ttggtagctt cttctacagt agtgtgggaa
     1561 caagatgcag atccttttgc tggaacagcc gacgacttcc ccgccttcaa tgaagaggag
     1621 cttgcatggt tgatggagct gttacctcaa ggaggatcag ggggtctttt agatccaggt
     1681 actcccatgg atgcagacct ggtcgcttca agtacagtcg tttgggagca ggatgctgat
     1741 ccattcgcag gtacagcaga tgacttcccg gctttcaatg aggaggaact tgcttggctc
     1801 atggaattgc tcccgcaggc aagaggtgga tctggtggtc tgctcgaccc aggaacgccg
     1861 atggacgctg atctggtagc atctagtaca gtctgggagc aggacgctga cccattcgct
     1921 ggtacggctg acgattttcc tgcctttaat gaggaagagt tagcctggct gatggagtta
     1981 ttaccccagg gcggtagtgg cggattattg gaccctggta cgccgatgga tgcagacctt
     2041 gtggcttcat caacggtcgt atgggagcag gatgccgatc ctttcgcagg aaccgcagac
     2101 gacttcccag cctttaacga agaagagctc gcatggctta tggaactttt acctcaggca
     2161 cgtggaggaa gtggtgggct cctggatcca ggcactccga tggacgcaga cttagtagct
     2221 agttccaccg ttgtctggga gcaggatgca gatccttttg ctggtaccgc agacgacttc
     2281 cctgctttca atgaagaaga attggcctgg cttatggaat tacttccgca gggtgggtct
     2341 ggtgggctgc tcgatcccgg tacgccgatg gatgctgact tggttgctag cagtactgtg
     2401 gtctgggaac aggatgctga ccccttcgct gggactgccg acgacttccc agccttcaac
     2461 gaagaggaac tcgcatggtt gatggaactg cttcctcaag cacgtggggg gtcaggcgga
     2521 ggcggatctg gcggtgacgc actggacgac tttgatctcg acatgcttgg gtccgatgct
     2581 ctcgacgatt ttgacctcga tatgcttggg tctgacgcat tagatgactt tgatttagac
     2641 atgttggggt cagacgcttt ggacgatttc gacttagata tgttggccag aggtagtgat
     2701 gcccttgacg acttcgattt agatatgttg ggctctgacg ccctcgatga cttcgatctg
     2761 gatatgctcg gaagtgacgc cttggacgat ttcgacttgg acatgcttgg gtcggacgcc
     2821 ctcgacgact ttgatctgga tatgttatga gcttggaatg gatcttcgat cccgatcgtt
     2881 caaacatttg gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgacgatta
     2941 tcatataatt tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt
     3001 tatttatgag atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag
     3061 aaaacaaaat atagcgcgca aactaggata aattatcgcg cdcggtgtca tctatgttac
     3121 tagatcggga attgccaagc taattcttga agacgaaagg gcctcgtgat acgcctattt
     3181 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     3241 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     3301 atgagacaat aaccctgata aatgcttcaa taatgggacc gactcgcgct tgagacgaag
     3361 ctttgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt agaataatcg
     3421 gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc
     3481 acagggttcc cctcgggatc aaagtacttt gatccaaccc ctccgctgct atagtgcagt
     3541 cggcttctga cgttcagtgc agccgtcatc tgaaaacgac atgtcgcaca agtcctaagt
     3601 tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg
     3661 cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca agagcgccgc
     3721 cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga ccaaccaacg
     3781 ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca ccggcaccag
     3841 gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta cgccctggcg acgttgtgac
     3901 agtgaccagg ctagaccgcc tggcccgcag cacccgcgac ctactggaca ttgccgagcg
     3961 catccaggag gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg acaccaccac
     4021 gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg agcgttccct
     4081 aatcatcgac cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg
     4141 cccccgccct accctcaccc cggcacagat cgcgcacgcc cgcgagctga tcgaccagga
     4201 aggccgcacc gtgaaagagg cggctgcact gcttggcgtg catcgctcga ccctgtaccg
     4261 cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg
     4321 tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac gccaagagga
     4381 acaagcatga aaccgcacca ggacggccag gacgaaccgt ttttcattac cgaagagatc
     4441 gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacct ctcaaccgtg
     4501 cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg gccggccagc
     4561 ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac
     4621 agcttgcgtc atgcggtcgc tgcgtatatg atccgatgag taaataaaca aatacgcaag
     4681 gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc aagacgacca
     4741 tcggaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg ttagtcgatt
     4801 ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg
     4861 ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc cggcgcgact
     4921 tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag
     4981 ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc accgccgacc
     5041 tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa gcggcctttg
     5101 tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag gcgctggccg
     5161 ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac ccaggcactg
     5221 ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc
     5281 aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta aagagaaaat
     5341 gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc
     5401 aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc agttgccggc
     5461 ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca ttaccgagct
     5521 gctatctgaa tagatcgcgc agctaccaga gtaaatgagc aaatgaataa atgagtagat
     5581 gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc accgacgccg
     5641 tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc tgggttgtct
     5701 gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga cggtcgcaaa
     5761 ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg acctggtgga gaagttgaag
     5821 gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg tgaatcgtgg
     5881 caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc cggtgcgccg
     5941 tcgattagga agccgcccaa gggcgacgag caaccagatt ttttcgttcc gatgctctat
     6001 gacgtgggca cccgcgatag tcgcagcatc atggacgtgg ccgttttccg tctgtcgaag
     6061 cgtgaccgac gagctggcga ggtgatccgc tacgagcttc cagacgggca cgtagaggtt
     6121 tccgcagggc cggccggcat ggccagtgtg tgggattacg acctggtact gatggcggtt
     6181 tcccatctaa ccgaatccat gaaccgatac cgggaaggga agggagacaa gcccggccgc
     6241 gtgttccgtc cacacgttgc ggacgtactc aagttctgcc ggcgagccga tggcggaaag
     6301 cagaaagacg acctggtaga aacctgcatt cggttaaaca ccacgcacgt tgccatgcag
     6361 cgtacgaaga aggccaagaa cggccgcctg gtgacggtat ccgagggtga agccttgatt
     6421 agccgctaca agatcgtaaa gagcgaaacc gggcggccgg agtacatcga gatcgagcta
     6481 gctgattgga tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct gacggttcac
     6541 cccgattact ttttgatcga tcccggcatc ggccgttttc tctaccgcct ggcacgccgc
     6601 gccgcaggca aggcagaagc cagatggttg ttcaagacga tctacgaacg cagtggcagc
     6661 gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc aaatgacctg
     6721 ccggagtacg atttgaagga ggaggcgggg caggctggcc cgatcctagt catgcgctac
     6781 cgcaacctga tcgagggcga agcatccgcc ggttcctaat gtacggagca gatgctaggg
     6841 caaattgccc tagcagggga aaaaggtcga aaaggactct ttcctgtgga tagcacgtac
     6901 attgggaacc caaagccgta cattgggaac cggaacccgt acattgggaa cccaaagccg
     6961 tacattggga accggtcaca catgtaagtg actgatataa aagagaaaaa aggcgatttt
     7021 tccgcctaaa actctttaaa acttattaaa actcttaaaa cccgcctggc ctgtgcataa
     7081 ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc ctacccttcg gtcgctgcgc
     7141 tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc aaaaatggct
     7201 ggcctacggc caggcaatct accagggcgc ggacaagccg cgccgtcgcc actcgaccgc
     7261 cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt gatgacggtg aaaacctctg
     7321 acacatgcag ctcccggtga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     7381 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc
     7441 acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg
     7501 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
     7561 aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     7621 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     7681 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     7741 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     7801 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     7861 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     7921 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     7981 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     8041 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     8101 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     8161 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     8221 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     8281 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     8341 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     8401 attttggtca tgcattctag gtgattagaa aaactcatcg agcatcaaat gaaactgcaa
     8461 tttattcata tcaggattat caataccata tttttgaaaa agccgtttct gtaatgaagg
     8521 agaaaactca ccgaggcagt tccataggat ggcaagatcc tggtatcggt ctgcgattcc
     8581 gactcgtcca acatcaatac aacctattaa tttcccctcg tcaaaaataa ggttatcaag
     8641 tgagaaatca ccatgagtga cgactgaatc cggtgagaat ggcaaaagtt tatgcatttc
     8701 tttccagact tgttcaacag gccagccatt acgctcgtca tcaaaatcac tcgcatcaac
     8761 caaaccgtta ttcattcgtg attgcgcctg agcgagtcga aatacgcgat cgctgttaaa
     8821 aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac
     8881 aatattttca cctgaatcag gatattcttc taatacctgg aatgctgttt tccctgggat
     8941 cgcagtggtg agtaaccatg catcatcagg agtacggata aaatgcttga tggtcggaag
     9001 aggcataaat tccgtcagcc agtttagtct gaccatctca tctgtaacat cattggcaac
     9061 gctacctttg ccatgtttca gaaacaactc tggcgcatcg ggcttcccat acaatcggta
     9121 gattgtcgca cctgattgcc cgacattatc gcgagcccat ttatacccat ataaatcagc
     9181 atccatgttg gaatttaatc gcggccttga gcaagacgtt tcccgttgaa tatggctcat
     9241 aacagaactt attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta
     9301 attataacaa gacgaactcc aattcactgt tccttgcatt ctaaaacctt aaataccaga
     9361 aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga
     9421 ttttgaaacc gcggtgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta
     9481 ccctccgcga gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag
     9541 catcggtaac atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg
     9601 gactgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg
     9661 ttggctggct ggtggcagga tatattgtgg tgtaaaca
//
