LOCUS       sequence-31099-e         632 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Nishino J, Kim I, Chada K, Morrison SJ
  TITLE     Hmga2 promotes neural stem cell self-renewal in young but not old 
            mice by reducing p16Ink4a and p19Arf Expression.
  JOURNAL   Cell. 2008 Oct 17. 135(2):227-39.
  PUBMED    18957199
REFERENCE   2  (bases 1 to 632)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2008 
            Oct 17. 135(2):227-39."
COMMENT     Sequence Label: HMGA2 shRNA
FEATURES             Location/Qualifiers
     source          1..632
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      15..24
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(63..266)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(92..112)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        267..570
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ctcgcccttg ctcaccatgg tggcgaccgg tagcgctagc ggatctgacg gttcactaaa
       61 ccagctctgc ttatatagac ctcccaccgt acacgcctac cgcccatttg cgtcaatggg
      121 gcggagttgt tacgacattt tggaaagtcc cgttgatttt ggtgccaaaa caaactccca
      181 ttgacgtcaa tggggtggag acttggaaat ccccgtgagt caaaccgcta tccacgccca
      241 ttgatgtact gccaaaaccg catcaccatg gtaatagcga tgactaatac gtagatgtac
      301 tgccaagtag gaaagtccca taaggtcatg tactgggcat aatgccaggc gggccattta
      361 ccgtcattga cgtcaatagg gggcgtactt ggcatatgat acacttgatg tactgccaag
      421 tgggcagttt accgtaaata ctccacccat tgacgtcaat ggaaagtccc tattggcgtt
      481 actatgggaa catacgtcat tattgacgtc aatgggcggg ggtcgttggg cggtcagcca
      541 ggcgggccat ttaccgtaag ttatgtaacg cggaactcca tatatgggct atgaactaat
      601 gaccccgtaa ttgattacta ttaataacta at
//
