LOCUS       sequence-310991-       10313 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TU for the expression of chimeric construct comprising the 
            N-terminal fragment of A. thaliana PhyB (amino acids 1-650) fused to
            the VP16 activation domain and a nuclear localization sequence. Part
            of a red light-controlled synthetic genetic switch.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10313)
  TITLE     Orzaez lab plasmids
REFERENCE   2  (bases 1 to 10313)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pEGB3_alpha2 35:PhyB:VP16  (GB2745)
FEATURES             Location/Qualifiers
     source          1..10313
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        637..981
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     925..945
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             3208..3441
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             3442..3462
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      3489..3741
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3776..3794)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    3979..4003
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5304..5933
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIGTHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             6367..7434
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      7500..7694
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7930..7952)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    8038..8178
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     8093..8112
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8193..8210)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8364..8952)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8444..8463)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9040..9855)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(9040..9855)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     9763..9782
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    10288..10312
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 taacaagctt cgtctcagtc aggagactag agccaagctg atctcctttg ccccggagat
       61 caccatggac gactttctct atctctacga tctaggaaga aagttcgacg gagaaggtga
      121 cgataccatg ttcaccaccg ataatgagaa gattagcctc ttcaatttca gaaagaatgc
      181 tgacccacag atggttagag aggcctacgc ggcaggtctg atcaagacga tctacccgag
      241 taataatctc caggagatca aataccttcc caagaaggtt aaagatgcag tcaaaagatt
      301 caggactaac tgcatcaaga acacagagaa agatatattt ctcaagatca gaagtactat
      361 tccagtatgg acgattcaag gcttgcttca taaaccaagg caagtaatag agattggagt
      421 ctctaagaaa gtagttccta ctgaatcaaa ggccatggag tcaaaaattc agatcgagga
      481 tctaacagaa ctcgccgtga agactggcga acagttcata cagagtcttt tacgactcaa
      541 tgacaagaag aaaatcttcg tcaacatggt ggagcacgac actctcgtct actccaagaa
      601 tatcaaagat acagtctcag aagaccaaag ggctattgag acttttcaac aaagggtaat
      661 atcgggaaac ctcctcggat tccattgccc agctatctgt cacttcatca aaaggacagt
      721 agaaaaggaa ggtggcacct acaaatgcca tcattgcgat aaaggaaagg ctatcgttca
      781 agatgcctct gccgacagtg gtcccaaaga tggaccccca cccacgagga gcatcgtgga
      841 aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgata tctccactga
      901 cgtaagggat gacgcacaat cccactatcc ttcgcaagac ccttcctcta tataaggaag
      961 ttcatttcat ttggagagga ctccggtatt tttacaacaa taccacaaca aaacaaacaa
     1021 caaacaacat tacaatttac tattctagtc gaaatgacaa caatggtttc cggagtcggg
     1081 ggtagtggcg gtggccgtgg cggtggccgt ggcggagaag aagaaccgtc gtcaagtcac
     1141 actcctaata accgaagagg aggagaacaa gctcaatcgt cgggaacgaa atctctcaga
     1201 ccaagaagca acactgaatc aatgagcaaa gcaattcaac agtacaccgt cgacgcaaga
     1261 ctccacgccg ttttcgaaca atccggcgaa tcagggaaat cattcgacta ctcacaatca
     1321 ctcaaaacga cgacgtacgg ttcctctgta cctgagcaac agatcacagc ttatctctct
     1381 cgaatccagc gaggtggtta cattcagcct ttcggatgta tgatcgccgt cgatgaatcc
     1441 agtttccgga tcatcggtta cagtgaaaac gccagagaaa tgttagggat tatgcctcaa
     1501 tctgttccta ctcttgagaa acctgagatt ctagctatgg gaactgatgt gagatctttg
     1561 ttcacttctt cgagctcgat tctactcgag cgtgctttcg ttgctcgaga gattaccttg
     1621 ttaaatccgg tttggatcca ttccaagaat actggtaaac cgttttacgc cattcttcat
     1681 aggattgatg ttggtgttgt tattgattta gagccagcta gaactgaaga tcctgcgctt
     1741 tctattgctg gtgctgttca atcgcagaaa ctcgcggttc gtgcgatttc tcagttacag
     1801 gctcttcctg gtggagatat taagcttttg tgtgacactg tcgtggaaag tgtgagggac
     1861 ttgactggtt atgatcgtgt tatggtttat aagtttcatg aagatgagca tggagaagtt
     1921 gtagctgaga gtaaacgaga tgatttagag ccttatattg gactgcatta tcctgctact
     1981 gatattcctc aagcgtcaag gttcttgttt aagcagaacc gtgtccgaat gatagtagat
     2041 tgcaatgcca cacctgttct tgtggtccag gacgataggc taactcagtc tatgtgcttg
     2101 gttggttcta ctcttagggc tcctcatggt tgtcactctc agtatatggc taacatggga
     2161 tctattgcgt ctttagcaat ggcggttata atcaatggaa atgaagatga tgggagcaat
     2221 gtagctagtg gaagaagctc gatgaggctt tggggtttgg ttgtttgcca tcacacttct
     2281 tctcgctgca taccgtttcc gctaaggtat gcttgtgagt ttttgatgca ggctttcggt
     2341 ttacagttaa acatggaatt gcagttagct ttgcaaatgt cagagaaacg cgttttgaga
     2401 acgcagacac tgttatgtga tatgcttctg cgtgactcgc ctgctggaat tgttacacag
     2461 agtcccagta tcatggactt agtgaaatgt gacggtgcag catttcttta ccacgggaag
     2521 tattacccgt tgggtgttgc tcctagtgaa gttcagataa aagatgttgt ggagtggttg
     2581 cttgcgaatc atgcggattc aaccggatta agcactgata gtttaggcga tgcggggtat
     2641 cccggtgcag ctgcgttagg ggatgctgtg tgcggtatgg cagttgcata tatcacaaaa
     2701 agagactttc ttttttggtt tcgatctcac actgcgaaag aaatcaaatg gggaggcgct
     2761 aagcatcatc cggaggataa agatgatggg caacgaatgc atcctcgttc gtcctttcag
     2821 gcttttcttg aagttgttaa gagccggagt cagccatggg aaactgcgga aatggatgcg
     2881 attcactcgc tccagcttat tctgagagac tcttttaaag aatctgaggc ggctatgaac
     2941 tctaaagttg tggatggtgt ggttcagcca tgtagggata tggcggggga acaggggatt
     3001 gatgagttag gtgaattcga tagtgctggt agtgctggta gtgctggttc gggttccgcg
     3061 tacagccgcg cgcgtacgaa aaacaattac gggtctacca tcgagggcct gctcgatctc
     3121 ccggacgacg acgcccccga agaggcgggg ctggcggctc cgcgcctgtc ctttctcccc
     3181 gcgggacaca cgcgcagact gtcgacggcc cccccgaccg atgtcagcct gggggacgag
     3241 ctccacttag acggcgagga cgtggcgatg gcgcatgccg acgcgctaga cgatttcgat
     3301 ctggacatgt tgggggacgg ggattccccg ggtccgggat ttacccccca cgactccgcc
     3361 ccctacggcg ctctggatat ggccgacttc gagtttgagc agatgtttac cgatgccctt
     3421 ggaattgacg agtacggtgg gccaaagaag aagaggaagg tgtaggcttg gaatggatct
     3481 tcgatcccga tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg
     3541 gtcttgcgac gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca
     3601 tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg caattataca
     3661 tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcdcgg
     3721 tgtcatctat gttactagat cgggaattgc caagctaatt cttgaagacg aaagggcctc
     3781 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     3841 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     3901 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataatg ggaccgactc
     3961 gcgcttgaga cgaagctttg acaggatata ttggcgggta aacctaagag aaaagagcgt
     4021 ttattagaat aatcggatat ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt
     4081 atgtgcatgc caaccacagg gttcccctcg ggatcaaagt actttgatcc aacccctccg
     4141 ctgctatagt gcagtcggct tctgacgttc agtgcagccg tcatctgaaa acgacatgtc
     4201 gcacaagtcc taagttacgc gacaggctgc cgccctgccc ttttcctggc gttttcttgt
     4261 cgcgtgtttt agtcgcataa agtagaatac ttgcgactag aaccggagac attacgccat
     4321 gaacaagagc gccgccgctg gcctgctggg ctatgcccgc gtcagcaccg acgaccagga
     4381 cttgaccaac caacgggccg aactgcacgc ggccggctgc accaagctgt tttccgagaa
     4441 gatcaccggc accaggcgcg accgcccgga gctggccagg atgcttgacc acctacgccc
     4501 tggcgacgtt gtgacagtga ccaggctaga ccgcctggcc cgcagcaccc gcgacctact
     4561 ggacattgcc gagcgcatcc aggaggccgg cgcgggcctg cgtagcctgg cagagccgtg
     4621 ggccgacacc accacgccgg ccggccgcat ggtgttgacc gtgttcgccg gcattgccga
     4681 gttcgagcgt tccctaatca tcgaccgcac ccggagcggg cgcgaggccg ccaaggcccg
     4741 aggcgtgaag tttggccccc gccctaccct caccccggca cagatcgcgc acgcccgcga
     4801 gctgatcgac caggaaggcc gcaccgtgaa agaggcggct gcactgcttg gcgtgcatcg
     4861 ctcgaccctg taccgcgcac ttgagcgcag cgaggaagtg acgcccaccg aggccaggcg
     4921 gcgcggtgcc ttccgtgagg acgcattgac cgaggccgac gccctggcgg ccgccgagaa
     4981 tgaacgccaa gaggaacaag catgaaaccg caccaggacg gccaggacga accgtttttc
     5041 attaccgaag agatcgaggc ggagatgatc gcggccgggt acgtgttcga gccgcccgcg
     5101 cacctctcaa ccgtgcggct gcatgaaatc ctggccggtt tgtctgatgc caagctggcg
     5161 gcctggccgg ccagcttggc cgctgaagaa accgagcgcc gccgtctaaa aaggtgatgt
     5221 gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt atatgatccg atgagtaaat
     5281 aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt acttaaccag aaaggcgggt
     5341 caggcaagac gaccatcgga acccatctag cccgcgccct gcaactcgcc ggggccgatg
     5401 ttctgttagt cgattccgat ccccagggca gtgcccgcga ttgggcggcc gtgcgggaag
     5461 atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat tgaccgcgac gtgaaggcca
     5521 tcggccggcg cgacttcgta gtgatcgacg gagcgcccca ggcggcggac ttggctgtgt
     5581 ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca gccaagccct tacgacatat
     5641 gggccaccgc cgacctggtg gagctggtta agcagcgcat tgaggtcacg gatggaaggc
     5701 tacaagcggc ctttgtcgtg tcgcgggcga tcaaaggcac gcgcatcggc ggtgaggttg
     5761 ccgaggcgct ggccgggtac gagctgccca ttcttgagtc ccgtatcacg cagcgcgtga
     5821 gctacccagg cactgccgcc gccggcacaa ccgttcttga atcagaaccc gagggcgacg
     5881 ctgcccgcga ggtccaggcg ctggccgctg aaattaaatc aaaactcatt tgagttaatg
     5941 aggtaaagag aaaatgagca aaagcacaaa cacgctaagt gccggccgtc cgagcgcacg
     6001 cagcagcaag gctgcaacgt tggccagcct ggcagacacg ccagccatga agcgggtcaa
     6061 ctttcagttg ccggcggagg atcacaccaa gctgaagatg tacgcggtac gccaaggcaa
     6121 gaccattacc gagctgctat ctgaatagat cgcgcagcta ccagagtaaa tgagcaaatg
     6181 aataaatgag tagatgaatt ttagcggcta aaggaggcgg catggaaaat caagaacaac
     6241 caggcaccga cgccgtggaa tgccccatgt gtggaggaac gggcggttgg ccaggcgtaa
     6301 gcggctgggt tgtctgccgg ccctgcaatg gcactggaac ccccaagccc gaggaatcgg
     6361 cgtgacggtc gcaaaccatc cggcccggta caaatcggcg cggcgctggg tgatgacctg
     6421 gtggagaagt tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc agaagcacgc
     6481 cccggtgaat cgtggcaagc ggccgctgat cgaatccgca aagaatcccg gcaaccgccg
     6541 gcagccggtg cgccgtcgat taggaagccg cccaagggcg acgagcaacc agattttttc
     6601 gttccgatgc tctatgacgt gggcacccgc gatagtcgca gcatcatgga cgtggccgtt
     6661 ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga tccgctacga gcttccagac
     6721 gggcacgtag aggtttccgc agggccggcc ggcatggcca gtgtgtggga ttacgacctg
     6781 gtactgatgg cggtttccca tctaaccgaa tccatgaacc gataccggga agggaaggga
     6841 gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt ctgccggcga
     6901 gccgatggcg gaaagcagaa agacgacctg gtagaaacct gcattcggtt aaacaccacg
     6961 cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac ggtatccgag
     7021 ggtgaagcct tgattagccg ctacaagatc gtaaagagcg aaaccgggcg gccggagtac
     7081 atcgagatcg agctagctga ttggatgtac cgcgagatca cagaaggcaa gaacccggac
     7141 gtgctgacgg ttcaccccga ttactttttg atcgatcccg gcatcggccg ttttctctac
     7201 cgcctggcac gccgcgccgc aggcaaggca gaagccagat ggttgttcaa gacgatctac
     7261 gaacgcagtg gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg caagctgatc
     7321 gggtcaaatg acctgccgga gtacgatttg aaggaggagg cggggcaggc tggcccgatc
     7381 ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc ctaatgtacg
     7441 gagcagatgc tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg actctttcct
     7501 gtggatagca cgtacattgg gaacccaaag ccgtacattg ggaaccggaa cccgtacatt
     7561 gggaacccaa agccgtacat tgggaaccgg tcacacatgt aagtgactga tataaaagag
     7621 aaaaaaggcg atttttccgc ctaaaactct ttaaaactta ttaaaactct taaaacccgc
     7681 ctggcctgtg cataactgtc tggccagcgc acagccgaag agctgcaaaa agcgcctacc
     7741 cttcggtcgc tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc ggccgctggc
     7801 cgctcaaaaa tggctggcct acggccaggc aatctaccag ggcgcggaca agccgcgccg
     7861 tcgccactcg accgccggcg cccacatcaa ggcaccctgc ctcgcgcgtt tcggtgatga
     7921 cggtgaaaac ctctgacaca tgcagctccc ggtgacggtc acagcttgtc tgtaagcgga
     7981 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc
     8041 agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca
     8101 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
     8161 agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     8221 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     8281 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     8341 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     8401 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     8461 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     8521 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     8581 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     8641 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     8701 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     8761 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     8821 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     8881 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     8941 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     9001 aactcacgtt aagggatttt ggtcatgcat tctaggtgat tagaaaaact catcgagcat
     9061 caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg
     9121 tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta
     9181 tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa
     9241 aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa
     9301 aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa
     9361 atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gtcgaaatac
     9421 gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac
     9481 tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc
     9541 tgttttccct gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg
     9601 cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt
     9661 aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt
     9721 cccatacaat cggtagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata
     9781 cccatataaa tcagcatcca tgttggaatt taatcgcggc cttgagcaag acgtttcccg
     9841 ttgaatatgg ctcataacag aacttattat ttccttcctc ttttctacag tatttaaaga
     9901 taccccaaga agctaattat aacaagacga actccaattc actgttcctt gcattctaaa
     9961 accttaaata ccagaaaaca gctttttcaa agttgttttc aaagttggcg tataacatag
    10021 tatcgacgga gccgattttg aaaccgcggt gatcacaggc agcaacgctc tgtcatcgtt
    10081 acaatcaaca tgctaccctc cgcgagatca tccgtgtttc aaacccggca gcttagttgc
    10141 cgttcttccg aatagcatcg gtaacatgag caaagtctgc cgccttacaa cggctctccc
    10201 gctgacgccg tcccggactg atgggctgcc tgtatcgagt ggtgattttg tgccgagctg
    10261 ccggtcgggg agctgttggc tggctggtgg caggatatat tgtggtgtaa aca
//
