LOCUS       sequence-311002-       11733 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module for estradiol-inducible exp of the PhiC31 integrase gene. 
            Includes TU for constitutive exp of an estradiol-inducible 
            transcription activator and TU for estradiol-inducible exp of 
            PhiC31..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11733)
  AUTHORS   Bernabe-Orts JM, Quijano-Rubio A, Vazquez-Vilar M, Mancheno-Bonillo 
            J, Moles-Casas V, Selma S, Gianoglio S, Granell A, Orzaez D
  TITLE     A memory switch for plant synthetic biology based on the phage 
            varphiC31 integration system.
  JOURNAL   Nucleic Acids Res. 2020 Apr 6;48(6):3379-3394. doi: 
            10.1093/nar/gkaa104.
  PUBMED    32083668
REFERENCE   2  (bases 1 to 11733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2020-04-6- 6"; volume: "48"; issue: "6";
            pages: "3379-3394"
COMMENT     Sequence Label: 
            pDGB3_Omega1_Pnos:ER:lexABD:GAL4AD:T35s-OplexA:mini35S:phi31:Tnos 
            (GB1677)
FEATURES             Location/Qualifiers
     source          1..11733
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        83..262
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             304..1239
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPGKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             352..1242
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPGKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATV"
     CDS             1243..1494
                     /codon_start=1
                     /gene="lexA (truncated)"
                     /product="DNA-binding domain of E. coli LexA"
                     /label=LexA BD
                     /note="binds the 16-bp palindromic sequence 
                     5'-CTGTATATATATACAG-3'"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAA"
     terminator      1812..2064
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        2576..2622
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             2697..4511
                     /codon_start=1
                     /product="integrase from phage phi-C31"
                     /label=phage phi-C31 integrase
                     /translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG
                     GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP
                     IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
                     GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
                     IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
                     RDPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
                     WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
                     QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
                     NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
                     APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
                     EKRASITWAKPPTDDDEDDAQDGTEDVAA"
     CDS             4512..4532
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      4559..4811
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    5053..5077
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6378..7007
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIGTHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7441..8508
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      8574..8768
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(9004..9026)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9112..9252
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9167..9186
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9267..9284)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9438..10026)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9518..9537)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10114..10905)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    11691..11715
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ggagatacat gagaattaag ggagtcacgg tatgaccccc gccgatgacg cgggacaagc
       61 cgttttacgt ttggaactga cagaaccgca acgttgaagg agccactgag ccgcgggttt
      121 ctggagttta atgagctaag cacatacgtc agaaaccatt attgcgcgtt caaaagtcgc
      181 ctaaggtcac tatcagctag caaatatttc ttgtcaaaaa tgctccactg acgttccata
      241 aattcccctc ggtatccaat tagagtctca tattcactct caactcgatc gaggcccatg
      301 tctgctggag acatgagagc tgccaacctt tggccaagcc cgctcatgat caaacgctct
      361 aagaagaaca gcctggcctt gtccctgacg gccgaccaga tggtcagtgc cttgttggat
      421 gctgagcccc ccatactcta ttccgagtat gatcctacca gacccttcag tgaagcttcg
      481 atgatgggct tactgaccaa cctggcagac agggagctgg ttcacatgat caactgggcg
      541 aagagggtgc caggctttgt ggatttgacc ctccatgatc aggtccacct tctagaatgt
      601 gcctggctag agatcctgat gattggactc gtctggcgct ccatggagca cccagggaag
      661 ctactgtttg ctcctaactt gctcttggac aggaaccagg gaaaatgtgt agagggcatg
      721 gtggagatct tcgacatgct gctggctaca tcatctcggt tccgcatgat gaatctgcag
      781 ggagaggagt ttgtgtgcct caaatctatt attttgctta attctggagt gtacacattt
      841 ctgtccagca ccctgaagtc tctggaagag aaggaccata tccaccgagt cctggacaag
      901 atcacagaca ctttgatcca cctgatggcc aaggcaggcc tgaccctgca gcagcagcac
      961 cagcggctgg cccagctcct cctcatcctc tcccacatca ggcacatgag taacaaaggc
     1021 atggagcatc tgtacagcat gaagtgcaag aacgtggtgc ccctctatga cctgctgctg
     1081 gagatgctgg acgcccaccg cctacatgcg cccactagcc gtggaggggc atccgtggag
     1141 gaaacggacc aaagccactt ggccactgcg ggctctactt cgtcgcattc cttgcaaaag
     1201 tattacatca cgggggaggc agagggtttc cctgccacag taatgaaagc gttaacggcc
     1261 aggcaacaag aggtgtttga tctcatccgt gatcacatca gccagacagg tatgccgccg
     1321 acgcgtgcgg aaatcgcgca gcgtttgggg ttccgttccc caaacgcggc tgaagaacat
     1381 ctgaaggcgc tggcacgcaa aggcgttatt gaaattgttt ccggcgcatc acgcgggatt
     1441 cgtctgttgc aggaagagga agaagggttg ccgctggtag gtcgtgtggc tgccggtgaa
     1501 ccggcaggtg gcagtggagg gggcggaagc gggggtacaa attctcaagc gctttcacaa
     1561 ccaattgcct cctctaacgt tcatgataac ttcatgaata atgaaatcac ggctagtaaa
     1621 attgatgatg gtaataattc aaaaccactg tcacctggtt ggacggacca aactgcgtat
     1681 aacgcgtttg gaatcactac agggatgttt aataccacta caatggatga tgtatataac
     1741 tatctattcg atgatgaaga taccccacca aacccaaaaa aagagtgagc ttggaatgga
     1801 tcttcgatcc cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg
     1861 ccggtcttgc gacgattatc atataatttc tgttgaatta cgttaagcat gtaataatta
     1921 acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat
     1981 acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcd
     2041 cggtgtcatc tatgttacta gatcgggaat tgccaagcta attcttgaag acgaaagggc
     2101 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     2161 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     2221 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atgggaccga
     2281 ctcgcgctgt caggagagct tgggctgcag gtcgaggcta aaaaactaat cgcattatca
     2341 tcccctcgac gtactgtaca tataaccact ggttttatat acagcagtac tgtacatata
     2401 accactggtt ttatatacag cagtcgacgt actgtacata taaccactgg ttttatatac
     2461 agcagtactg tacatataac cactggtttt atatacagca gtcgaggtaa gattagatat
     2521 ggatatgtat atggatatgt atatggtggt aatgccatgt aatatgctcc ccttcgcaag
     2581 acccttcctc tatataagga agttcatttc atttggagag gactccggta tttttacaac
     2641 aattaccaca acaaaacaaa caacaaacaa cattacaatt tactattcta gtcgaaatgg
     2701 acacgtatgc cggcgcttat gatagacaat caagagaaag agagaactct tctgccgcct
     2761 ctccagctac acagagatct gccaacgagg ataaagctgc tgatttacaa agagaggtag
     2821 agagagatgg gggtaggttt agatttgtgg gtcatttctc tgaggctcct ggtacatcag
     2881 cctttggtac tgctgaacgg cctgaatttg aaagaatatt aaatgagtgt cgggccggtc
     2941 gactaaatat gataatagtt tatgatgtat ctaggttcag tagactcaaa gttatggatg
     3001 caattccgat cgtgagcgag ctacttgcac tcggtgttac tatcgtaagt acacaagaag
     3061 gtgtattcag gcagggtaat gtgatggacc ttatacacct tattatgcga ctcgacgcta
     3121 gccataaaga gtcatcactg aagagtgcta aaatcttgga caccaagaac ctgcaaaggg
     3181 agcttggagg ctatgtagga ggaaaagctc cgtatggttt cgaactcgtg agtgagacaa
     3241 aagagattac cagaaatggt aggatggtga acgtggttat taataagcta gctcatagca
     3301 caacccctct cacagggcct tttgagttcg aacctgacgt tattagatgg tggtggagag
     3361 agatcaaaac tcacaaacat cttccattta agccaggtag ccaggctgct atccaccctg
     3421 gatctatcac tgggctttgt aaaaggatgg atgctgacgc agtgccaact cgaggggaaa
     3481 caatcggtaa gaagactgca tcttccgctt gggatcccgc tacagttatg agaatccttc
     3541 gggatccgcg tattgctgga ttcgccgcag aagtcattta taagaaaaaa cctgatggta
     3601 ctcctactac aaaaatcgaa ggttatagga tccaaaggga tcctattact ttgcgtccag
     3661 ttgaattaga ctgtggaccg atcatcgaac ctgccgagtg gtacgagttg caggcctggc
     3721 ttgatggtag agggcgtgga aagggtttat ctcggggaca ggcaattttg tcagcaatgg
     3781 ataagcttta ttgtgagtgt ggggctgtta tgacatcaaa acgtggagaa gagtcaatca
     3841 aggattctta caggtgcaga cgtcgtaaag tggtggaccc ttctgctcca ggacagcatg
     3901 agggtacatg caacgtttct atggctgctc ttgataaatt tgtggccgaa cgtattttca
     3961 acaagattag acacgctgaa ggggatgagg aaactttagc tcttctttgg gaggctgccc
     4021 ggcgttttgg taagctaaca gaggcaccag aaaaaagtgg cgaaagagct aatttggtag
     4081 ccgaaagagc agacgccttg aatgcattgg aagaacttta cgaagatagg gctgccggag
     4141 catatgatgg accagttggt cggaagcatt tcaggaagca acaagctgca ctaactttga
     4201 gacagcaagg cgcagaggag cgactcgcag agcttgaggc cgccgaggcc ccaaagcttc
     4261 cactcgatca atggtttcct gaggatgccg acgctgatcc tacaggtcca aagagctggt
     4321 ggggaagagc tagcgtagac gataagagag tgtttgtggg tctatttgtt gacaagattg
     4381 tagttactaa gtccactacg ggtcgaggac agggcactcc catcgaaaag agagccagta
     4441 ttacctgggc aaagcctcca accgatgatg atgaagacga tgcacaagat ggtaccgagg
     4501 atgtggcagc accaaaaaaa aagaggaagg tgtaagcttg gaatggatct tcgatcccga
     4561 tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgac
     4621 gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat
     4681 gacgttattt atgagatggg tttttatgat tagagtcccg caattataca tttaatacgc
     4741 gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcdcgg tgtcatctat
     4801 gttactagat cgggaattgc caagctaatt cttgaagacg aaagggcctc gtgatacgcc
     4861 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     4921 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     4981 cgctcatgag acaataaccc tgataaatgc ttcaataatg ggaccgactc gcgctgtcat
     5041 gagaccggat cctgacagga tatattggcg ggtaaaccta agagaaaaga gcgtttatta
     5101 gaataatcgg atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc
     5161 atgccaacca cagggttccc ctcgggatca aagtactttg atccaacccc tccgctgcta
     5221 tagtgcagtc ggcttctgac gttcagtgca gccgtcatct gaaaacgaca tgtcgcacaa
     5281 gtcctaagtt acgcgacagg ctgccgccct gcccttttcc tggcgttttc ttgtcgcgtg
     5341 ttttagtcgc ataaagtaga atacttgcga ctagaaccgg agacattacg ccatgaacaa
     5401 gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc accgacgacc aggacttgac
     5461 caaccaacgg gccgaactgc acgcggccgg ctgcaccaag ctgttttccg agaagatcac
     5521 cggcaccagg cgcgaccgcc cggagctggc caggatgctt gaccacctac gccctggcga
     5581 cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc acccgcgacc tactggacat
     5641 tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc ctggcagagc cgtgggccga
     5701 caccaccacg ccggccggcc gcatggtgtt gaccgtgttc gccggcattg ccgagttcga
     5761 gcgttcccta atcatcgacc gcacccggag cgggcgcgag gccgccaagg cccgaggcgt
     5821 gaagtttggc ccccgcccta ccctcacccc ggcacagatc gcgcacgccc gcgagctgat
     5881 cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg cttggcgtgc atcgctcgac
     5941 cctgtaccgc gcacttgagc gcagcgagga agtgacgccc accgaggcca ggcggcgcgg
     6001 tgccttccgt gaggacgcat tgaccgaggc cgacgccctg gcggccgccg agaatgaacg
     6061 ccaagaggaa caagcatgaa accgcaccag gacggccagg acgaaccgtt tttcattacc
     6121 gaagagatcg aggcggagat gatcgcggcc gggtacgtgt tcgagccgcc cgcgcacctc
     6181 tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg atgccaagct ggcggcctgg
     6241 ccggccagct tggccgctga agaaaccgag cgccgccgtc taaaaaggtg atgtgtattt
     6301 gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga tccgatgagt aaataaacaa
     6361 atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc gggtcaggca
     6421 agacgaccat cggaacccat ctagcccgcg ccctgcaact cgccggggcc gatgttctgt
     6481 tagtcgattc cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg gaagatcaac
     6541 cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag gccatcggcc
     6601 ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc ggacttggct gtgtccgcga
     6661 tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag cccttacgac atatgggcca
     6721 ccgccgacct ggtggagctg gttaagcagc gcattgaggt cacggatgga aggctacaag
     6781 cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag gttgccgagg
     6841 cgctggccgg gtacgagctg cccattcttg agtcccgtat cacgcagcgc gtgagctacc
     6901 caggcactgc cgccgccggc acaaccgttc ttgaatcaga acccgagggc gacgctgccc
     6961 gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact catttgagtt aatgaggtaa
     7021 agagaaaatg agcaaaagca caaacacgct aagtgccggc cgtccgagcg cacgcagcag
     7081 caaggctgca acgttggcca gcctggcaga cacgccagcc atgaagcggg tcaactttca
     7141 gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag gcaagaccat
     7201 taccgagctg ctatctgaat agatcgcgca gctaccagag taaatgagca aatgaataaa
     7261 tgagtagatg aattttagcg gctaaaggag gcggcatgga aaatcaagaa caaccaggca
     7321 ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc gtaagcggct
     7381 gggttgtctg ccggccctgc aatggcactg gaacccccaa gcccgaggaa tcggcgtgac
     7441 ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag
     7501 aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc acgccccggt
     7561 gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc
     7621 ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc aaccagattt tttcgttccg
     7681 atgctctatg acgtgggcac ccgcgatagt cgcagcatca tggacgtggc cgttttccgt
     7741 ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct acgagcttcc agacgggcac
     7801 gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt gggattacga cctggtactg
     7861 atggcggttt cccatctaac cgaatccatg aaccgatacc gggaagggaa gggagacaag
     7921 cccggccgcg tgttccgtcc acacgttgcg gacgtactca agttctgccg gcgagccgat
     7981 ggcggaaagc agaaagacga cctggtagaa acctgcattc ggttaaacac cacgcacgtt
     8041 gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa
     8101 gccttgatta gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag
     8161 atcgagctag ctgattggat gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg
     8221 acggttcacc ccgattactt tttgatcgat cccggcatcg gccgttttct ctaccgcctg
     8281 gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat ctacgaacgc
     8341 agtggcagcg ccggagagtt caagaagttc tgtttcaccg tgcgcaagct gatcgggtca
     8401 aatgacctgc cggagtacga tttgaaggag gaggcggggc aggctggccc gatcctagtc
     8461 atgcgctacc gcaacctgat cgagggcgaa gcatccgccg gttcctaatg tacggagcag
     8521 atgctagggc aaattgccct agcaggggaa aaaggtcgaa aaggactctt tcctgtggat
     8581 agcacgtaca ttgggaaccc aaagccgtac attgggaacc ggaacccgta cattgggaac
     8641 ccaaagccgt acattgggaa ccggtcacac atgtaagtga ctgatataaa agagaaaaaa
     8701 ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc
     8761 tgtgcataac tgtctggcca gcgcacagcc gaagagctgc aaaaagcgcc tacccttcgg
     8821 tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta tcgcggccgc tggccgctca
     8881 aaaatggctg gcctacggcc aggcaatcta ccagggcgcg gacaagccgc gccgtcgcca
     8941 ctcgaccgcc ggcgcccaca tcaaggcacc ctgcctcgcg cgtttcggtg atgacggtga
     9001 aaacctctga cacatgcagc tcccggtgac ggtcacagct tgtctgtaag cggatgccgg
     9061 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat
     9121 gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag
     9181 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     9241 taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     9301 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     9361 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     9421 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     9481 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     9541 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     9601 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     9661 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     9721 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     9781 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     9841 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     9901 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     9961 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
    10021 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
    10081 cgttaaggga ttttggtcat gcattctagg tgattatttg ccgactacct tggtgatctc
    10141 gcctttcacg tagtggacaa attcttccaa ctgatctgcg cgcgaggcca agcgatcttc
    10201 ttcttgtcca agataagcct gtctagcttc aagtatgacg ggctgatact gggccggcag
    10261 gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg attttgccgg ttactgcgct
    10321 gtaccaaatg cgggacaacg taagcactac atttcgctca tcaccagccc agtcgggcgg
    10381 cgagttccat agcgttaagg tttcatttag cgcctcaaat agatcctgtt caggaaccgg
    10441 atcaaagagt tcctccgccg ctggacctac caaggcaacg ctatgttctc ttgcttttgt
    10501 cagcaagata gccagatcaa tgtcgatcgt ggctggctcg aagatacctg caagaatgtc
    10561 attgcgctgc cattctccaa attgcagttc gcgcttagct ggataacgcc acggaatgat
    10621 gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga atctcgctct ctccagggga
    10681 agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc gttgtttcat caagccttac
    10741 ggtcaccgta accagcaaat caatatcact gtgtggcttc aggccgccat ccactgcgga
    10801 gccgtacaaa tgtacggcca gcaacgtcgg ttcgagatgg cgctcgatga cgccaactac
    10861 ctctgatagt tgagtcgata cttcggcgat caccgcttcc ctcataatgt ttaactttgt
    10921 tttagggcga ctgccctgct gcgtaacatc gttgctgctc cataacatca aacatcgacc
    10981 cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata gactgtaccc caaaaaaaca
    11041 gtcataacaa gccatgaaaa ccgccactgc gccgttacca ccgctgcgtt cggtcaaggt
    11101 tctggaccag ttgcgtgagc gcatacgcta cttgcattac agcttacgaa ccgaacaggc
    11161 ttatgtccac tgggttcgtg ccttcatccg tttccacggt gtgcgtcacc cggcaacctt
    11221 gggtagcagc gaagtcgagg catttctgtc ctggctggaa cagaacttat tatttccttc
    11281 ctcttttcta cagtatttaa agatacccca agaagctaat tataacaaga cgaactccaa
    11341 ttcactgttc cttgcattct aaaaccttaa ataccagaaa acagcttttt caaagttgtt
    11401 ttcaaagttg gcgtataaca tagtatcgac ggagccgatt ttgaaaccgc ggtgatcaca
    11461 ggcagcaacg ctctgtcatc gttacaatca acatgctacc ctccgcgaga tcatccgtgt
    11521 ttcaaacccg gcagcttagt tgccgttctt ccgaatagca tcggtaacat gagcaaagtc
    11581 tgccgcctta caacggctct cccgctgacg ccgtcccgga ctgatgggct gcctgtatcg
    11641 agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt ggctggctgg tggcaggata
    11701 tattgtggtg taaacataac ggatccggtc tca
//
