LOCUS       sequence-311008-       13528 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  module containing the human codon optimized Cas9 and the BASTA 
            selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13528)
  TITLE     Orzaez lab plasmids
REFERENCE   2  (bases 1 to 13528)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pEGB3 omega1 Tnos:BASTA:Pnos - P35S:hCas9:Tnos...
FEATURES             Location/Qualifiers
     source          1..13528
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(41..293)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(313..864)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(906..1085)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        1791..2135
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     2079..2099
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             2208..6311
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             2208..6311
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             2208..6311
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6324..6344
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      6371..6623
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(6658..6676)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    6865..6889
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8190..8819
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9253..10320
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      10386..10580
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10816..10838)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10924..11064
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10979..10998
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(11079..11096)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11250..11838)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11330..11349)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11926..12717)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    13503..13527
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 taacggatcc ggtctcagga gatcagcttg catgccggtc gatctagtaa catagatgac
       61 accgcgcgcg ataatttatc ctagtttgcg cgctatattt tgttttctat cgcgtattaa
      121 atgtataatt gcgggactct aatcataaaa acccatctca taaataacgt catgcattac
      181 atgttaatta ttacatgctt aacgtaattc aacagaaatt atatgataat catcccaaga
      241 ccggcaacag gattcaatct taagaaactt tattgccaaa tgtttgaacg atctgcttga
      301 ctctaggggt catcagattt cggtgacggg caggaccgga cggggcggca ccggcaggct
      361 gaagtccagc tgccagaaac ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg
      421 cagcatgccg cggggggcat atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg
      481 cagcccgatg acagcgacca cgctcttgaa gccctgtgcc tccagggact tcagcaggtg
      541 ggtgtagagc gtggagccca gtcccgtccg ctggtggcgg ggggacacgt acacggtcga
      601 ctcggccgtc cagtcgtagg cgttgcgtgc cttccaggga cccgcgtagg ctatgccggc
      661 gacctcgccg tccacctcgg cgacgagcca gggatagcgc tcccgcagac ggacgaggtc
      721 gtccgtccac tcctgcggtt cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat
      781 gtagtggttg acgatggtgc agaccgccgg catgtccgcc tcggtggcac ggcggatgtc
      841 ggccgggcgt cgttctgggc tcatggtaga tcccctcgat cgagttgaga gtgaatatga
      901 gactctaatt ggataccgag gggaatttat ggaacgtcag tggagcattt ttgacaagaa
      961 atatttgcta gctgatagtg accttaggcg acttttgaac gcgcaataat ggtttctgac
     1021 gtatgtgctt agctcattaa actccagaaa cccgcggctc agtggctcct tcaacgttgc
     1081 ggttctgtca gttccaaacg taaaacggct tgtcccgcgt catcggcggg ggtcataacg
     1141 tgactccctt aattctcatg tatgatacgc tgtcaggaga ctagagccaa gctgatctcc
     1201 tttgccccgg agatcaccat ggacgacttt ctctatctct acgatctagg aagaaagttc
     1261 gacggagaag gtgacgatac catgttcacc accgataatg agaagattag cctcttcaat
     1321 ttcagaaaga atgctgaccc acagatggtt agagaggcct acgcggcagg tctgatcaag
     1381 acgatctacc cgagtaataa tctccaggag atcaaatacc ttcccaagaa ggttaaagat
     1441 gcagtcaaaa gattcaggac taactgcatc aagaacacag agaaagatat atttctcaag
     1501 atcagaagta ctattccagt atggacgatt caaggcttgc ttcataaacc aaggcaagta
     1561 atagagattg gagtctctaa gaaagtagtt cctactgaat caaaggccat ggagtcaaaa
     1621 attcagatcg aggatctaac agaactcgcc gtgaagactg gcgaacagtt catacagagt
     1681 cttttacgac tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca cgacactctc
     1741 gtctactcca agaatatcaa agatacagtc tcagaagacc aaagggctat tgagactttt
     1801 caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat ctgtcacttc
     1861 atcaaaagga cagtagaaaa ggaaggtggc acctacaaat gccatcattg cgataaagga
     1921 aaggctatcg ttcaagatgc ctctgccgac agtggtccca aagatggacc cccacccacg
     1981 aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt ggattgatgt
     2041 gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca agacccttcc
     2101 tctatataag gaagttcatt tcatttggag aggactccgg tatttttaca acaataccac
     2161 aacaaaacaa acaacaaaca acattacaat ttactattct agtcgaaatg gacaagaagt
     2221 actccattgg gctcgatatc ggcacaaaca gcgtcggctg ggccgtcatt acggacgagt
     2281 acaaggtgcc gagcaaaaaa ttcaaagttc tgggcaatac cgatcgccac agcataaaga
     2341 agaacctcat tggcgccctc ctgttcgact ccggggaaac ggccgaagcc acgcggctca
     2401 aaagaacagc acggcgcaga tatacccgca gaaagaatcg gatctgctac ctgcaggaga
     2461 tctttagtaa tgagatggct aaggtggatg actctttctt ccataggctg gaggagtcct
     2521 ttttggtgga ggaggataaa aagcacgagc gccacccaat ctttggcaat atcgtggacg
     2581 aggtggcgta ccatgaaaag tacccaacca tatatcatct gaggaagaag cttgtagaca
     2641 gtactgataa ggctgacttg cggttgatct atctcgcgct ggcgcatatg atcaaatttc
     2701 ggggacactt cctcatcgag ggggacctga acccagacaa cagcgatgtc gacaaactct
     2761 ttatccaact ggttcagact tacaatcagc ttttcgaaga gaacccgatc aacgcatccg
     2821 gagttgacgc caaagcaatc ctgagcgcta ggctgtccaa atcccggcgg ctcgaaaacc
     2881 tcatcgcaca gctccctggg gagaagaaga acggcctgtt tggtaatctt atcgccctgt
     2941 cactcgggct gacccccaac tttaaatcta acttcgacct ggccgaagat gccaagcttc
     3001 aactgagcaa agacacctac gatgatgatc tcgacaatct gctggcccag atcggcgacc
     3061 agtacgcaga cctttttttg gcggcaaaga acctgtcaga cgccattctg ctgagtgata
     3121 ttctgcgagt gaacacggag atcaccaaag ctccgctgag cgctagtatg atcaagcgct
     3181 atgatgagca ccaccaagac ttgactttgc tgaaggccct tgtcagacag caactgcctg
     3241 agaagtacaa ggaaattttc ttcgatcagt ctaaaaatgg ctacgccgga tacattgacg
     3301 gcggagcaag ccaggaggaa ttttacaaat ttattaagcc catcttggaa aaaatggacg
     3361 gcaccgagga gctgctggta aagcttaaca gagaagatct gttgcgcaaa cagcgcactt
     3421 tcgacaatgg aagcatcccc caccagattc acctgggcga actgcacgct atcctcaggc
     3481 ggcaagagga tttctacccc tttttgaaag ataacaggga aaagattgag aaaatcctca
     3541 catttcggat accctactat gtaggccccc tcgcccgggg aaattccaga ttcgcgtgga
     3601 tgactcgcaa atcagaagag actatcactc cctggaactt cgaggaagtc gtggataagg
     3661 gggcctctgc ccagtccttc atcgaaagga tgactaactt tgataaaaat ctgcctaacg
     3721 aaaaggtgct tcctaaacac tctctgctgt acgagtactt cacagtttat aacgagctca
     3781 ccaaggtcaa atacgtcaca gaagggatga gaaagccagc attcctgtct ggagagcaga
     3841 agaaagctat cgtggacctc ctcttcaaga cgaaccggaa agttaccgtg aaacagctca
     3901 aagaagatta tttcaaaaag attgaatgtt tcgactctgt tgaaatcagc ggagtggagg
     3961 atcgcttcaa cgcatccctg ggaacgtatc acgatctcct gaaaatcatt aaagacaagg
     4021 acttcctgga caatgaggag aacgaggaca ttcttgagga cattgtcctc acccttacgt
     4081 tgtttgaaga tagggagatg attgaagaac gcttgaaaac ttacgctcat ctcttcgacg
     4141 acaaagtcat gaaacagctc aagaggcgcc gatatacagg atgggggcgg ctgtcaagaa
     4201 aactgatcaa tgggatccga gacaagcaga gtggaaagac aatcctggat tttcttaagt
     4261 ccgatggatt tgccaaccgg aacttcatgc agttgatcca tgatgactct ctcaccttta
     4321 aggaggacat ccagaaagca caagtttctg gccaggggga cagtctccac gagcacatcg
     4381 ctaatcttgc aggtagccca gctatcaaaa agggaatact gcagaccgtt aaggtcgtgg
     4441 atgaactcgt caaagtaatg ggaaggcata agcccgagaa tatcgttatc gagatggccc
     4501 gagagaacca aactacccag aagggacaga agaacagtag ggaaaggatg aagaggattg
     4561 aagagggtat aaaagaactg gggtcccaaa tccttaagga acacccagtt gaaaacaccc
     4621 agcttcagaa tgagaagctc tacctgtact acctgcagaa cggcagggac atgtacgtgg
     4681 atcaggaact ggacatcaat cggctctccg actacgacgt ggatcatatc gtgccccagt
     4741 cttttctcaa agatgattct attgataata aagtgttgac aagatccgat aaaaatagag
     4801 ggaagagtga taacgtcccc tcagaagaag ttgtcaagaa aatgaaaaat tattggcggc
     4861 agctgctgaa cgccaaactg atcacacaac ggaagttcga taatctgact aaggctgaac
     4921 gaggtggcct gtctgagttg gataaagccg gcttcatcaa aaggcagctt gttgagacac
     4981 gccagatcac caagcacgtg gcccaaattc tcgattcacg catgaacacc aagtacgatg
     5041 aaaatgacaa actgattcga gaggtgaaag ttattactct gaagtctaag ctggtttcag
     5101 atttcagaaa ggactttcag ttttataagg tgagagagat caacaattac caccatgcgc
     5161 atgatgccta cctgaatgca gtggtaggca ctgcacttat caaaaaatat cccaagcttg
     5221 aatctgaatt tgtttacgga gactataaag tgtacgatgt taggaaaatg atcgcaaagt
     5281 ctgagcagga aataggcaag gccaccgcta agtacttctt ttacagcaat attatgaatt
     5341 ttttcaagac cgagattaca ctggccaatg gagagattcg gaagcgacca cttatcgaaa
     5401 caaacggaga aacaggagaa atcgtgtggg acaagggtag ggatttcgcg acagtccgga
     5461 aggtcctgtc catgccgcag gtgaacatcg ttaaaaagac cgaagtacag accggaggct
     5521 tctccaagga aagtatcctc ccgaaaagga acagcgacaa gctgatcgca cgcaaaaaag
     5581 attgggaccc caagaaatac ggcggattcg attctcctac agtcgcttac agtgtactgg
     5641 ttgtggccaa agtggagaaa gggaagtcta aaaaactcaa aagcgtcaag gaactgctgg
     5701 gcatcacaat catggagcga tcaagcttcg aaaaaaaccc catcgacttt ctcgaggcga
     5761 aaggatataa agaggtcaaa aaagacctca tcattaagct tcccaagtac tctctctttg
     5821 agcttgaaaa cggccggaaa cgaatgctcg ctagtgcggg cgagctgcag aaaggtaacg
     5881 agctggcact gccctctaaa tacgttaatt tcttgtatct ggccagccac tatgaaaagc
     5941 tcaaaggatc tcccgaagat aatgagcaga agcagctgtt cgtggaacaa cacaaacact
     6001 accttgatga gatcatcgag caaataagcg aattctccaa aagagtgatc ctcgccgacg
     6061 ctaacctcga taaggtgctt tctgcttaca ataagcacag ggataagccc atcagggagc
     6121 aggcagaaaa cattatccac ttgtttactc tgaccaactt gggcgcgcct gcagccttca
     6181 agtacttcga caccaccata gacagaaagc ggtacacctc tacaaaggag gtcctggacg
     6241 ccacactgat tcatcagtca attacggggc tctatgaaac aagaatcgac ctctctcagc
     6301 tcggtggaga cagcagggct gaccccaaga agaagaggaa ggtgtgagct tggaatggat
     6361 cttcgatccc gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc
     6421 cggtcttgcg acgattatca tataatttct gttgaattac gttaagcatg taataattaa
     6481 catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata
     6541 catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcdc
     6601 ggtgtcatct atgttactag atcgggaatt gccaagctaa ttcttgaaga cgaaagggcc
     6661 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     6721 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     6781 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tgggaccgac
     6841 tcgcgctgtc atgagaccgg atcctgacag gatatattgg cgggtaaacc taagagaaaa
     6901 gagcgtttat tagaataatc ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc
     6961 atttgtatgt gcatgccaac cacagggttc ccctcgggat caaagtactt tgatccaacc
     7021 cctccgctgc tatagtgcag tcggcttctg acgttcagtg cagccgtcat ctgaaaacga
     7081 catgtcgcac aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt cctggcgttt
     7141 tcttgtcgcg tgttttagtc gcataaagta gaatacttgc gactagaacc ggagacatta
     7201 cgccatgaac aagagcgccg ccgctggcct gctgggctat gcccgcgtca gcaccgacga
     7261 ccaggacttg accaaccaac gggccgaact gcacgcggcc ggctgcacca agctgttttc
     7321 cgagaagatc accggcacca ggcgcgaccg cccggagctg gccaggatgc ttgaccacct
     7381 acgccctggc gacgttgtga cagtgaccag gctagaccgc ctggcccgca gcacccgcga
     7441 cctactggac attgccgagc gcatccagga ggccggcgcg ggcctgcgta gcctggcaga
     7501 gccgtgggcc gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt tcgccggcat
     7561 tgccgagttc gagcgttccc taatcatcga ccgcacccgg agcgggcgcg aggccgccaa
     7621 ggcccgaggc gtgaagtttg gcccccgccc taccctcacc ccggcacaga tcgcgcacgc
     7681 ccgcgagctg atcgaccagg aaggccgcac cgtgaaagag gcggctgcac tgcttggcgt
     7741 gcatcgctcg accctgtacc gcgcacttga gcgcagcgag gaagtgacgc ccaccgaggc
     7801 caggcggcgc ggtgccttcc gtgaggacgc attgaccgag gccgacgccc tggcggccgc
     7861 cgagaatgaa cgccaagagg aacaagcatg aaaccgcacc aggacggcca ggacgaaccg
     7921 tttttcatta ccgaagagat cgaggcggag atgatcgcgg ccgggtacgt gttcgagccg
     7981 cccgcgcacc tctcaaccgt gcggctgcat gaaatcctgg ccggtttgtc tgatgccaag
     8041 ctggcggcct ggccggccag cttggccgct gaagaaaccg agcgccgccg tctaaaaagg
     8101 tgatgtgtat ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat gatgcgatga
     8161 gtaaataaac aaatacgcaa ggggaacgca tgaaggttat cgctgtactt aaccagaaag
     8221 gcgggtcagg caagacgacc atcgcaaccc atctagcccg cgccctgcaa ctcgccgggg
     8281 ccgatgttct gttagtcgat tccgatcccc agggcagtgc ccgcgattgg gcggccgtgc
     8341 gggaagatca accgctaacc gttgtcggca tcgaccgccc gacgattgac cgcgacgtga
     8401 aggccatcgg ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg gcggacttgg
     8461 ctgtgtccgc gatcaaggca gccgacttcg tgctgattcc ggtgcagcca agcccttacg
     8521 acatatgggc caccgccgac ctggtggagc tggttaagca gcgcattgag gtcacggatg
     8581 gaaggctaca agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc atcggcggtg
     8641 aggttgccga ggcgctggcc gggtacgagc tgcccattct tgagtcccgt atcacgcagc
     8701 gcgtgagcta cccaggcact gccgccgccg gcacaaccgt tcttgaatca gaacccgagg
     8761 gcgacgctgc ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa ctcatttgag
     8821 ttaatgaggt aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg gccgtccgag
     8881 cgcacgcagc agcaaggctg caacgttggc cagcctggca gacacgccag ccatgaagcg
     8941 ggtcaacttt cagttgccgg cggaggatca caccaagctg aagatgtacg cggtacgcca
     9001 aggcaagacc attaccgagc tgctatctga atacatcgcg cagctaccag agtaaatgag
     9061 caaatgaata aatgagtaga tgaattttag cggctaaagg aggcggcatg gaaaatcaag
     9121 aacaaccagg caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag
     9181 gcgtaagcgg ctgggttgtc tgccggccct gcaatggcac tggaaccccc aagcccgagg
     9241 aatcggcgtg acggtcgcaa accatccggc ccggtacaaa tcggcgcggc gctgggtgat
     9301 gacctggtgg agaagttgaa ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa
     9361 gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa
     9421 ccgccggcag ccggtgcgcc gtcgattagg aagccgccca agggcgacga gcaaccagat
     9481 tttttcgttc cgatgctcta tgacgtgggc acccgcgata gtcgcagcat catggacgtg
     9541 gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg ctacgagctt
     9601 ccagacgggc acgtagaggt ttccgcaggg ccggccggca tggccagtgt gtgggattac
     9661 gacctggtac tgatggcggt ttcccatcta accgaatcca tgaaccgata ccgggaaggg
     9721 aagggagaca agcccggccg cgtgttccgt ccacacgttg cggacgtact caagttctgc
     9781 cggcgagccg atggcggaaa gcagaaagac gacctggtag aaacctgcat tcggttaaac
     9841 accacgcacg ttgccatgca gcgtacgaag aaggccaaga acggccgcct ggtgacggta
     9901 tccgagggtg aagccttgat tagccgctac aagatcgtaa agagcgaaac cgggcggccg
     9961 gagtacatcg agatcgagct agctgattgg atgtaccgcg agatcacaga aggcaagaac
    10021 ccggacgtgc tgacggttca ccccgattac tttttgatcg atcccggcat cggccgtttt
    10081 ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag ccagatggtt gttcaagacg
    10141 atctacgaac gcagtggcag cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag
    10201 ctgatcgggt caaatgacct gccggagtac gatttgaagg aggaggcggg gcaggctggc
    10261 ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc cggttcctaa
    10321 tgtacggagc agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg aaaaggactc
    10381 tttcctgtgg atagcacgta cattgggaac ccaaagccgt acattgggaa ccggaacccg
    10441 tacattggga acccaaagcc gtacattggg aaccggtcac acatgtaagt gactgatata
    10501 aaagagaaaa aaggcgattt ttccgcctaa aactctttaa aacttattaa aactcttaaa
    10561 acccgcctgg cctgtgcata actgtctggc cagcgcacag ccgaagagct gcaaaaagcg
    10621 cctacccttc ggtcgctgcg ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc
    10681 gctggccgct caaaaatggc tggcctacgg ccaggcaatc taccagggcg cggacaagcc
    10741 gcgccgtcgc cactcgaccg ccggcgccca catcaaggca ccctgcctcg cgcgtttcgg
    10801 tgatgacggt gaaaacctct gacacatgca gctcccggtg acggtcacag cttgtctgta
    10861 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
    10921 gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg
    10981 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
    11041 gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc
    11101 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
    11161 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
    11221 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
    11281 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
    11341 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
    11401 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
    11461 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
    11521 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
    11581 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
    11641 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
    11701 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
    11761 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
    11821 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
    11881 aacgaaaact cacgttaagg gattttggtc atgcattcta ggtgattatt tgccgactac
    11941 cttggtgatc tcgcctttca cgtagtggac aaattcttcc aactgatctg cgcgcgaggc
    12001 caagcgatct tcttcttgtc caagataagc ctgtctagct tcaagtatga cgggctgata
    12061 ctgggccggc aggcgctcca ttgcccagtc ggcagcgaca tccttcggcg cgattttgcc
    12121 ggttactgcg ctgtaccaaa tgcgggacaa cgtaagcact acatttcgct catcaccagc
    12181 ccagtcgggc ggcgagttcc atagcgttaa ggtttcattt agcgcctcaa atagatcctg
    12241 ttcaggaacc ggatcaaaga gttcctccgc cgctggacct accaaggcaa cgctatgttc
    12301 tcttgctttt gtcagcaaga tagccagatc aatgtcgatc gtggctggct cgaagatacc
    12361 tgcaagaatg tcattgcgct gccattctcc aaattgcagt tcgcgcttag ctggataacg
    12421 ccacggaatg atgtcgtcgt gcacaacaat ggtgacttct acagcgcgga gaatctcgct
    12481 ctctccaggg gaagccgaag tttccaaaag gtcgttgatc aaagctcgcc gcgttgtttc
    12541 atcaagcctt acggtcaccg taaccagcaa atcaatatca ctgtgtggct tcaggccgcc
    12601 atccactgcg gagccgtaca aatgtacggc cagcaacgtc ggttcgagat ggcgctcgat
    12661 gacgccaact acctctgata gttgagtcga tacttcggcg atcaccgctt ccctcataat
    12721 gtttaacttt gttttagggc gactgccctg ctgcgtaaca tcgttgctgc tccataacat
    12781 caaacatcga cccacggcgt aacgcgcttg ctgcttggat gcccgaggca tagactgtac
    12841 cccaaaaaaa cagtcataac aagccatgaa aaccgccact gcgccgttac caccgctgcg
    12901 ttcggtcaag gttctggacc agttgcgtga gcgcatacgc tacttgcatt acagcttacg
    12961 aaccgaacag gcttatgtcc actgggttcg tgccttcatc cgtttccacg gtgtgcgtca
    13021 cccggcaacc ttgggtagca gcgaagtcga ggcatttctg tcctggctgg aacagaactt
    13081 attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta attataacaa
    13141 gacgaactcc aattcactgt tccttgcatt ctaaaacctt aaataccaga aaacagcttt
    13201 ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga ttttgaaacc
    13261 gcggtgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctccgcga
    13321 gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac
    13381 atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg
    13441 ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct
    13501 ggtggcagga tatattgtgg tgtaaaca
//
