LOCUS       sequence-311044-        8625 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid is a ERK pathway reporter. It has CMV promoter driving 
            the expression of ERK-KTR fused to mStrawberry-pGK-BSD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8625)
  AUTHORS   Kaufman T, Nitzan E, Firestein N, Ginzberg MB, Iyengar S, Patel N, 
            Ben-Hamo R, Porat Z, Hunter J, Hilfinger A, Rotter V, Kafri R, 
            Straussman R
  TITLE     Visual barcodes for clonal-multiplexing of live microscopy-based 
            assays.
  JOURNAL   Nat Commun. 2022 May 18;13(1):2725. doi: 10.1038/s41467-022-30008-0.
  PUBMED    35585055
REFERENCE   2  (bases 1 to 8625)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-05-18"; volume: "13(1):2725. doi"; pages: " 
            10.1038/s41467-022-30008-0"
COMMENT     Sequence Label: ERK-KTR-mStrawberry reporter
FEATURES             Location/Qualifiers
     source          1..8625
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     505..529
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    613..637
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             895..1605
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent 
                     protein"
                     /label=mStrawberry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILTPNFTYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKMRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYIVGIKLDITSHNEDYTIVELYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1040..1058)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1308..1327
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    1647..1764
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1813..2323
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2242..2260
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2335..2730
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    2766..3354
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2819..2839)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3237..3248)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3426..3659
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3737..3858
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3774..3793)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3828..3847
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3898..4033
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4053..4072)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4054..4072)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4082..4099)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4082..4098)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4091..4113)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4240..4695
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4327..4346)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4537..4558
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4721..4825
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4826..4894,4895..5686)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5044..5063)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5857..6445
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6346..6365
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6599..6616
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6733..6754
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6769..6774,6775..6792,6793..6799)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6807..6823
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6812..6834
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6831..6847
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6831..6847
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6866..6886
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6868..6886
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6914..7140
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7141..7321
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7368..7493
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7986..8219
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8404..8448
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      541 ccatccacgc tgttttgacc tccatagaag acaccgactc tagtccagtg tggtggaatt
      601 ctgcagatat caacaagttt gtacaaaaaa gcaggctcca ccatgaaggg ccgaaagcct
      661 cgggacctgg aacttccact tagcccaagc ctgctgggtg gccagggacc tgaacggact
      721 ccaggatcag gaacaagctc tggtcttcag gcaccggggc cagcgctttc gcccagcaag
      781 cgtagcggtc tcgaggaccc agcgacgcca tcgaagaagc cacgtacccc atcggtgtcg
      841 tcaaggctgg agcgcctgac cctccagtcc agctttcaat tcccatccac tagtatggtg
      901 agcaagggcg aggagaataa catggccatc atcaaggagt tcatgcgctt caaggtgcgc
      961 atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
     1021 tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc
     1081 tgggacatcc taacccccaa cttcacctac ggctccaagg cctacgtgaa gcaccccgcc
     1141 gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg
     1201 aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag
     1261 ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag
     1321 aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg
     1381 aagggcgaga tcaagatgag gctgaagctg aaggacggcg gccactacga cgctgaggtc
     1441 aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacat cgtcggcatc
     1501 aagttggaca tcacctccca caacgaggac tacaccatcg tggaactgta cgaacgcgcc
     1561 gagggccgcc actccaccgg cggcatggac gagctgtaca agtaagaatt ctagatcttg
     1621 agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
     1681 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
     1741 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccactttgg
     1801 cgccggctcg agggggcccg ggttgcgcct tttccaaggc agccctgggt ttgcgcaggg
     1861 acgcggctgc tctgggcgtg gttccgggaa acgcagcggc gccgaccctg ggtctcgcac
     1921 attcttcacg tccgttcgca gcgtcacccg gatcttcgcc gctacccttg tgggcccccc
     1981 ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc ttgcggttcg cggcgtgccg
     2041 gacgtgacaa acggaagccg cacgtctcac tagtaccctc gcagacggac agcgccaggg
     2101 agcaatggca gcgcgccgac cgcgatgggc tgtggccaat agcggctgct cagcagggcg
     2161 cgccgagagc agcggccggg aaggggcggt gcgggaggcg gggtgtgggg cggtagtgtg
     2221 ggccctgttc ctgcccgcgc ggtgttccgc attctgcaag cctccggagc gcacgtcggc
     2281 agtcggctcc ctcgttgacc gaatcaccga cctctctccc cagggggtac caccatggcc
     2341 aagcctttgt ctcaagaaga atccaccctc attgaaagag caacggctac aatcaacagc
     2401 atccccatct ctgaagacta cagcgtcgcc agcgcagctc tctctagcga cggccgcatc
     2461 ttcactggtg tcaatgtata tcattttact gggggacctt gtgcagaact cgtggtgctg
     2521 ggcactgctg ctgctgcggc agctggcaac ctgacttgta tcgtcgcgat cggaaatgag
     2581 aacaggggca tcttgagccc ctgcggacgg tgccgacagg tgcttctcga tctgcatcct
     2641 gggatcaaag ccatagtgaa ggacagtgat ggacagccga cggcagttgg gattcgtgaa
     2701 ttgctgccct ctggttatgt gtgggagggc ctgcagctgc agtagtaaag acgcgttaag
     2761 tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     2821 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     2881 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     2941 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     3001 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     3061 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     3121 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     3181 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     3241 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     3301 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcgtcgac
     3361 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     3421 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg
     3481 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     3541 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     3601 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     3661 tacgtatagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
     3721 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     3781 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     3841 actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc
     3901 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     3961 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     4021 ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct
     4081 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     4141 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     4201 gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat
     4261 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     4321 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     4381 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     4441 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     4501 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     4561 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     4621 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     4681 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     4741 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     4801 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     4861 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     4921 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     4981 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     5041 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     5101 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     5161 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     5221 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     5281 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     5341 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     5401 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     5461 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     5521 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     5581 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     5641 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     5701 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     5761 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     5821 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     5881 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     5941 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     6001 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     6061 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     6121 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     6181 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     6241 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     6301 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     6361 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     6421 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     6481 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     6541 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     6601 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     6661 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     6721 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     6781 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     6841 ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga
     6901 gctgcaagct taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg
     6961 agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta
     7021 aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga
     7081 accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac
     7141 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     7201 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     7261 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     7321 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
     7381 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
     7441 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
     7501 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
     7561 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
     7621 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
     7681 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
     7741 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
     7801 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
     7861 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     7921 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     7981 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     8041 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     8101 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     8161 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     8221 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     8281 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     8341 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     8401 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     8461 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     8521 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     8581 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccccg
//
