LOCUS       sequence-311046-        8852 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid is a JNK pathway reporter. It has CMV promoter driving 
            the expression of JNK-KTR fused to mStrawberry-pGK-BSD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8852)
  AUTHORS   Kaufman T, Nitzan E, Firestein N, Ginzberg MB, Iyengar S, Patel N, 
            Ben-Hamo R, Porat Z, Hunter J, Hilfinger A, Rotter V, Kafri R, 
            Straussman R
  TITLE     Visual barcodes for clonal-multiplexing of live microscopy-based 
            assays.
  JOURNAL   Nat Commun. 2022 May 18;13(1):2725. doi: 10.1038/s41467-022-30008-0.
  PUBMED    35585055
REFERENCE   2  (bases 1 to 8852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-05-18"; volume: "13(1):2725. doi"; pages: " 
            10.1038/s41467-022-30008-0"
COMMENT     Sequence Label: JNK-KTR-mStrawberry reporter
FEATURES             Location/Qualifiers
     source          1..8852
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     505..529
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    894..918
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1119..1829
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent 
                     protein"
                     /label=mStrawberry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILTPNFTYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKMRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYIVGIKLDITSHNEDYTIVELYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1264..1282)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1532..1551
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    1871..1988
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2037..2547
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     CDS             2559..2954
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    2990..3578
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3043..3063)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3461..3472)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3650..3883
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3961..4082
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3998..4017)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4052..4071
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4122..4257
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4277..4296)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4278..4296)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4306..4323)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4306..4322)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4315..4337)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4464..4919
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4551..4570)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4761..4782
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4945..5049
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5050..5118,5119..5910)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5268..5287)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6081..6669
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6570..6589
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6823..6840
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6957..6978
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6993..6998,6999..7016,7017..7023)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7031..7047
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7036..7058
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7055..7071
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7055..7071
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7090..7110
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7092..7110
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7138..7364
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7365..7545
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7592..7717
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8210..8443
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8628..8672
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      541 ccatccacgc tgttttgacc tccatagaag acaccgactc tagtccagtg tggtggaatt
      601 ctgcagatat caacaagtgg atggcagcgc gccgaccgcg atgggctgtg gccaatagcg
      661 gctgctcagc agggcgcgcc gagagcagcg gccgggaagg ggcggtgcgg gaggcggggt
      721 gtggggcggt agtgtgggcc ctgttcctgc ccgcgcggtg ttccgcattc tgcaagcctc
      781 cggagcgcac gtcggcagtc ggctccctcg ttgaccgaat caccgacctc tctccccagg
      841 gggatccacc ggttcgtcga ctagtccagt gtggtggaat tctgcagata tcaacaagtt
      901 tgtacaaaaa agcaggctcc accatgagta accctaagat cctaaaacag agcatgacct
      961 tgaacctggc cgacccggtg ggcagtctga agccgcacct ccgcgccaag cgctcggccg
     1021 gtctcaacga agacgagtcg ccatctaaga agctggcgtc gccggaggtg tcgtcaaggc
     1081 tggagcgcct gaccctccag tccagcacta gtcgacccat ggtgagcaag ggcgaggaga
     1141 ataacatggc catcatcaag gagttcatgc gcttcaaggt gcgcatggag ggctccgtga
     1201 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
     1261 ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctaaccc
     1321 ccaacttcac ctacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact
     1381 tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
     1441 gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga
     1501 agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct
     1561 gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaaga
     1621 tgaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg
     1681 ccaagaagcc cgtgcagctg cccggcgcct acatcgtcgg catcaagttg gacatcacct
     1741 cccacaacga ggactacacc atcgtggaac tgtacgaacg cgccgagggc cgccactcca
     1801 ccggcggcat ggacgagctg tacaagtaag aattctagat cttgagacaa atggcagtat
     1861 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
     1921 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
     1981 aaaattttcg ggtttattac agggacagca gagatccact ttggcgccgg ctcgaggggg
     2041 cccgggttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg
     2101 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt
     2161 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct
     2221 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa
     2281 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc
     2341 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc
     2401 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc
     2461 gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt
     2521 gaccgaatca ccgacctctc tccccagggg gtaccaccat ggccaagcct ttgtctcaag
     2581 aagaatccac cctcattgaa agagcaacgg ctacaatcaa cagcatcccc atctctgaag
     2641 actacagcgt cgccagcgca gctctctcta gcgacggccg catcttcact ggtgtcaatg
     2701 tatatcattt tactggggga ccttgtgcag aactcgtggt gctgggcact gctgctgctg
     2761 cggcagctgg caacctgact tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga
     2821 gcccctgcgg acggtgccga caggtgcttc tcgatctgca tcctgggatc aaagccatag
     2881 tgaaggacag tgatggacag ccgacggcag ttgggattcg tgaattgctg ccctctggtt
     2941 atgtgtggga gggcctgcag ctgcagtagt aaagacgcgt taagtcgaca atcaacctct
     3001 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     3061 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     3121 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     3181 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     3241 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     3301 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     3361 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc
     3421 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     3481 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     3541 tcagacgagt cggatctccc tttgggccgc ctccccgcgt cgactttaag accaatgact
     3601 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     3661 attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac
     3721 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     3781 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     3841 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtacgta tagtagttca
     3901 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
     3961 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     4021 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     4081 tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc
     4141 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     4201 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     4261 ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt
     4321 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     4381 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     4441 gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     4501 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     4561 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     4621 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     4681 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     4741 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     4801 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     4861 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta
     4921 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     4981 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     5041 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     5101 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     5161 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     5221 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     5281 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     5341 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     5401 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     5461 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     5521 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     5581 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     5641 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     5701 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     5761 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     5821 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     5881 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     5941 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     6001 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     6061 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     6121 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     6181 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     6241 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     6301 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     6361 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     6421 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     6481 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     6541 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     6601 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     6661 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     6721 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     6781 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     6841 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     6901 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     6961 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     7021 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     7081 gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt
     7141 agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct
     7201 tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg
     7261 ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc
     7321 attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag
     7381 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     7441 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     7501 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
     7561 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
     7621 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     7681 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     7741 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     7801 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     7861 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     7921 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     7981 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     8041 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     8101 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     8161 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     8221 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
     8281 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     8341 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     8401 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     8461 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     8521 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     8581 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     8641 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     8701 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     8761 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     8821 cccacctccc aaccccgagg ggcggccgcc cg
//
