LOCUS       sequence-311051-        5169 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive expression of E-PIF6(1-100) in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5169)
  AUTHORS   Ochoa-Fernandez R, Abel NB, Wieland FG, Schlegel J, Koch LA, Miller 
            JB, Engesser R, Giuriani G, Brandl SM, Timmer J, Weber W, Ott T, 
            Simon R, Zurbriggen MD
  TITLE     Optogenetic control of gene expression in plants in the presence of 
            ambient white light.
  JOURNAL   Nat Methods. 2020 Jul;17(7):717-725. doi: 10.1038/s41592-020-0868-y.
            Epub 2020 Jun 29.
  PUBMED    32601426
REFERENCE   2  (bases 1 to 5169)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-020-0868-y"; journalName: "Nat Methods"; date: 
            "2020-07"; volume: "17"; issue: "7"; pages: "717-725"
COMMENT     Sequence Label: pMZ827
FEATURES             Location/Qualifiers
     source          1..5169
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..346
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     289..309
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1345..1365
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      1471..1723
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        complement(1774..1792)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1813..1829)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1813..1829)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1826..1848)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1837..1853
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1861..1867,1868..1885,1886..1891))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1906..1927
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2044..2061)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2215..2803)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2295..2314)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2974..3765,3766..3834))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3597..3616
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3835..3939)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3965..4420)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4102..4123)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4314..4333
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4547..4569
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4561..4578
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4562..4578
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4585..4604
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4585..4603
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat gccatcattg
      121 cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gatatcacca ctgacgtaag ggatgacgca caatcccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag agaacacggg ggactctagc
      361 gctaccggtt ggctaggtaa gcttggtacc acctgaacga cgcatatgac aacaatgccc
      421 cgccccaagc tcaagtccga tgacgaggta ctcgaggccg ccaccgtagt gctgaagcgt
      481 tgcggtccca tagagttcac gctcagcgga gtagcaaagg aggtggggct ctcccgcgca
      541 gcgttaatcc agcgcttcac caaccgcgat acgctgctgg tgaggatgat ggagcgcggc
      601 gtcgagcagg tgcggcatta cctgaatgcg ataccgatag gcgcagggcc gcaagggctc
      661 tgggaatttt tgcaggtgct cgttcggagc atgaacactc gcaacgactt ctcggtgaac
      721 tatctcatct cctggtacga gctccaggtg ccggagctac gcacgcttgc gatccagcgg
      781 aaccgcgcgg tggtggaggg gatccgcaag cgactgcccc caggtgctcc tgcggcagct
      841 gagttgctcc tgcactcggt catcgctggc gcgacgatgc agtgggccgt cgatccggat
      901 ggtgagctag ctgatcatgt gctggctcag atcgctgcca tcctgtgttt aatgtttccc
      961 gaacacgacg atttccaact cctccaggca catgcgtccg cgtacagctt aattaacagt
     1021 gctggtagtg ctggtagtgc tggtatgatg ttcttaccaa ccgattattg ttgcaggtta
     1081 agcgatcaag agtatatgga gcttgtgttt gagaatggcc agattcttgc aaagggccaa
     1141 agatccaacg tttctctgca taatcaacgt accaaatcga tcatggattt gtatgaggca
     1201 gagtataacg aggatttcat gaagagtatc atccatggtg gtggtggtgc catcacaaat
     1261 ctcggggaca cgcaggttgt tccacaaagt catgttgctg ctgcccatga aacaaacatg
     1321 ttggaaagca ataaacatgt tgacccaaag aagaagagga aggtgtaggc aacggctagc
     1381 acgacgtgat gcggcagcgg ccgaattccc cgggctcgag aagcttggat ccaccggatc
     1441 tagataactg atctcgaggc gaatttcccc gatcgttcaa acatttggca ataaagtttc
     1501 ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac
     1561 gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg
     1621 attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac
     1681 taggataaat tatcgcgcgc ggtgtcatct atgttactag atcgggaatt aattcgataa
     1741 gctagagatc cgtcaaggta ccagcttttg ttccctttag tgagggttaa tttcgagctt
     1801 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     1861 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     1921 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     1981 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     2041 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     2101 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     2161 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     2221 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     2281 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     2341 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     2401 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     2461 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     2521 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     2581 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     2641 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     2701 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     2761 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     2821 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     2881 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     2941 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     3001 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     3061 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     3121 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     3181 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     3241 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     3301 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     3361 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     3421 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     3481 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     3541 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     3601 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     3661 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     3721 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     3781 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     3841 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     3901 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     3961 acctaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
     4021 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
     4081 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     4141 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     4201 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     4261 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     4321 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
     4381 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc attcaggctg
     4441 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa
     4501 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt
     4561 tgtaaaacga cggccagtga attgtaatac gactcactat agggcgaatt ggagctccac
     4621 cgcggagcgg agaattaagg gagtcaggcc ttaattaaga gctcgcatgc ctgcaggtcc
     4681 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     4741 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     4801 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     4861 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     4921 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4981 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     5041 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     5101 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     5161 aagggcaat
//
