LOCUS       sequence-311054-        2274 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ""Rescue mfn2 expression by adding the guide-resistant-mutant mfn2 
            sequence"".
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Zhou W, Hsu AY, Wang Y, Syahirah R, Wang T, Jeffries J, Wang X, 
            Mohammad H, Seleem MN, Umulis D, Deng Q
  TITLE     Mitofusin 2 regulates neutrophil adhesive migration and the actin 
            cytoskeleton.
  JOURNAL   J Cell Sci. 2020 Sep 4;133(17). pii: jcs.248880. doi: 
            10.1242/jcs.248880.
  PUBMED    32788232
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2020-09-4"; volume: "133(17). pii: jcs.248880. doi"; 
            pages: " 10.1242/jcs.248880"
COMMENT     Sequence Label: plko-shmfn2-mfn2-Rescue
FEATURES             Location/Qualifiers
     source          1..2274
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             918..926
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 atgtccctgc tcttctctcg atgcaactct atcgtcacag tcaagaaaaa taagagacac
       61 atggctgagg tgaatgcatc cccacttaag cactttgtca ctgccaagaa gaagatcaat
      121 ggcatttttg agcagctggg ggcctacatc caggagagcg ccaccttcct tgaagacacg
      181 tacaggaatg cagaactgga ccccgttacc acagaagaac aggttctgga cgtcaaaggt
      241 tacctatcca aagtgagagg catcagtgag gtgctggctc ggaggcacat gaaagtggct
      301 ttttttggcc ggacgagcaa tgggaagagc accgtgatca atgccatgct ctgggacaaa
      361 gttctgccct ctgggattgg ccacaccacc aattgcttcc tgcgggtaga gggcacagat
      421 ggccatgagg cctttctcct taccgagggc tcagaggaaa agaggagtgc caagactgtg
      481 aaccagctgg cccatgccct ccaccaggac aagcagctcc atgccggcag cctagtgagt
      541 gtgatgtggc ccaactctaa gtgcccactt ctgaaggatg acctcgtttt gatggacagc
      601 cctggtattg atgtcaccac agagctggac agctggattg acaagttttg tctggatgct
      661 gatgtgtttg tgctggtggc caactcagag tccaccctga tgcagacgga aaagcacttc
      721 ttccacaagg tgagtgagcg tctctcccgg ccaaacatct tcatcctgaa caaccgctgg
      781 gatgcatctg cctcagagcc cgagtacatg gaggaggtgc ggcggcagca catggagcgt
      841 tgtaccagct tcctggtgga tgagctgggc gtggtggatc gatcccaggc cggggaccgc
      901 atcttctttg tgtctgctaa ggaggtgctc aacgccagga ttcagaaagc ccagggcatg
      961 cctgaaggag ggggcgctct cgcagaaggc tttcaagtga ggatgtttga gtttcagaat
     1021 tttgagagga gatttgagga gtgcatctcc cagtctgcag tgaagaccaa gtttgagcag
     1081 cacacggtcc gggccaagca gattgcagag gcggttcgac tcatcatgga ctccctgcac
     1141 atggcggctc gggagcaaca agtgtattgt gaagagatgc gtgaagagcg gcaagaccga
     1201 ctgaaattta ttgacaaaca gctggagctc ttggctcaag actataagct gcgaattaag
     1261 cagattacgg aggaagtgga gaggcaggtg tcgactgcaa tggccgagga gatcaggcgc
     1321 ctctctgtac tggtggacga ttaccagatg gacttccacc cttctccagt agtcctcaag
     1381 gtttataaga atgagctgca ccgccacata gaggaaggac tgggtcgaaa catgtctgac
     1441 cgctgctcca cggccatcac caactccctg cagaccatgc agcaggacat gatagatggc
     1501 ttgaaacccc tccttcctgt gtctgtgcgg agtcagatag acatgctggt cccacgccag
     1561 tgcttctccc tcaactatga cctaaactgt gacaagctgt gtgctgactt ccaggaagac
     1621 attgagttcc atttctctct cggatggacc atgctggtga ataggttcct gggccccaag
     1681 aacagccgtc gggccttgat gggctacaat gaccaggtcc agcgtcccat ccctctgacg
     1741 ccagccaacc ccagcatgcc cccactgcca cagggctcgc tcacccagga ggagttcatg
     1801 gtttccatgg ttaccggcct ggcctccttg acatccagga cctccatggg cattcttgtt
     1861 gttggaggag tggtgtggaa ggcagtgggc tggcggctca ttgccctctc ctttgggctc
     1921 tatggcctcc tctacgtcta tgagcgtctg acctggacca ccaaggccaa ggagagggcc
     1981 ttcaagcgcc agtttgtgga gcatgccagc gagaagctgc agcttgtcat cagctacact
     2041 ggctccaact gcagccacca agtccagcag gaactgtctg ggacctttgc tcatctgtgt
     2101 cagcaagttg acgtcacccg ggagaacctg gagcaggaaa ttgccgccat gaacaagaaa
     2161 attgaggttc ttgactcact tcagagcaaa gcaaagctgc tcaggaataa agccggttgg
     2221 ttggacagtg agctcaacat gttcacacac cagtacctgc agcccagcag ataa
//
