LOCUS       sequence-311055-        6612 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive expression of PhyB(1-650)-VP16 in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6612)
  AUTHORS   Ochoa-Fernandez R, Abel NB, Wieland FG, Schlegel J, Koch LA, Miller 
            JB, Engesser R, Giuriani G, Brandl SM, Timmer J, Weber W, Ott T, 
            Simon R, Zurbriggen MD
  TITLE     Optogenetic control of gene expression in plants in the presence of 
            ambient white light.
  JOURNAL   Nat Methods. 2020 Jul;17(7):717-725. doi: 10.1038/s41592-020-0868-y.
            Epub 2020 Jun 29.
  PUBMED    32601426
REFERENCE   2  (bases 1 to 6612)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-020-0868-y"; journalName: "Nat Methods"; date: 
            "2020-07"; volume: "17"; issue: "7"; pages: "717-725"
COMMENT     Sequence Label: pMZ828
FEATURES             Location/Qualifiers
     source          1..6612
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..346
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     289..309
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             2554..2787
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             2788..2808
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      2914..3166
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        complement(3217..3235)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3256..3272)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3256..3272)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3269..3291)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3280..3296
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3304..3310,3311..3328,3329..3334))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3349..3370
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3487..3504)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3658..4246)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3738..3757)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4417..5208,5209..5277))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5040..5059
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5278..5382)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5408..5863)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5545..5566)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5757..5776
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5990..6012
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6004..6021
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6005..6021
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6028..6047
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6028..6046
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat gccatcattg
      121 cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gatatcacca ctgacgtaag ggatgacgca caatcccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag agaacacggg ggactctagc
      361 gctaccggtt ggctaggtaa gcttggtacc acctgaacga cgcatatgac aacaatggtt
      421 tccggagtcg ggggtagtgg cggtggccgt ggcggtggcc gtggcggaga agaagaaccg
      481 tcgtcaagtc acactcctaa taaccgaaga ggaggagaac aagctcaatc gtcgggaacg
      541 aaatctctca gaccaagaag caacactgaa tcaatgagca aagcaattca acagtacacc
      601 gtcgacgcaa gactccacgc cgttttcgaa caatccggcg aatcagggaa atcattcgac
      661 tactcacaat cactcaaaac gacgacgtac ggttcctctg tacctgagca acagatcaca
      721 gcttatctct ctcgaatcca gcgaggtggt tacattcagc ctttcggatg tatgatcgcc
      781 gtcgatgaat ccagtttccg gatcatcggt tacagtgaaa acgccagaga aatgttaggg
      841 attatgcctc aatctgttcc tactcttgag aaacctgaga ttctagctat gggaactgat
      901 gtgagatctt tgttcacttc ttcgagctcg attctactcg agcgtgcttt cgttgctcga
      961 gagattacct tgttaaatcc ggtttggatc cattccaaga atactggtaa accgttttac
     1021 gccattcttc ataggattga tgttggtgtt gttattgatt tagagccagc tagaactgaa
     1081 gatcctgcgc tttctattgc tggtgctgtt caatcgcaga aactcgcggt tcgtgcgatt
     1141 tctcagttac aggctcttcc tggtggagat attaagcttt tgtgtgacac tgtcgtggaa
     1201 agtgtgaggg acttgactgg ttatgatcgt gttatggttt ataagtttca tgaagatgag
     1261 catggagaag ttgtagctga gagtaaacga gacgatttag agccttatat tggactgcat
     1321 tatcctgcta ctgatattcc tcaagcgtca aggttcttgt ttaagcagaa ccgtgtccga
     1381 atgatagtag attgcaatgc cacacctgtt cttgtggtcc aggacgatag gctaactcag
     1441 tctatgtgct tggttggttc tactcttagg gctcctcatg gttgtcactc tcagtatatg
     1501 gctaacatgg gatctattgc gtctttagca atggcggtta taatcaatgg aaatgaagat
     1561 gatgggagca atgtagctag tggaagaagc tcgatgaggc tttggggttt ggttgtttgc
     1621 catcacactt cttctcgctg cataccgttt ccgctaaggt atgcttgtga gtttttgatg
     1681 caggctttcg gtttacagtt aaacatggaa ttgcagttag ctttgcaaat gtcagagaaa
     1741 cgcgttttga gaacgcagac actgttatgt gatatgcttc tgcgtgactc gcctgctgga
     1801 attgttacac agagtcccag tatcatggac ttagtgaaat gtgacggtgc agcatttctt
     1861 taccacggga agtattaccc gttgggtgtt gctcctagtg aagttcagat aaaagatgtt
     1921 gtggagtggt tgcttgcgaa tcatgcggat tcaaccggat taagcactga tagtttaggc
     1981 gatgcggggt atcccggtgc agctgcgtta ggggatgctg tgtgcggtat ggcagttgca
     2041 tatatcacaa aaagagactt tcttttttgg tttcgatctc acactgcgaa agaaatcaaa
     2101 tggggaggcg ctaagcatca tccggaggat aaagatgatg ggcaacgaat gcatcctcgt
     2161 tcgtcctttc aggcttttct tgaagttgtt aagagccgga gtcagccatg ggaaactgcg
     2221 gaaatggatg cgattcactc gctccagctt attctgagag actcttttaa agaatctgag
     2281 gcggctatga actctaaagt tgtggatggt gtggttcagc catgtaggga tatggcgggg
     2341 gaacagggga ttgatgagtt aggtgaattc gatagtgctg gtagtgctgg tagtgctggt
     2401 tccgcgtaca gccgcgcgcg tacgaaaaac aattacgggt ctaccatcga gggcctgctc
     2461 gatctcccgg acgacgacgc ccccgaagag gcggggctgg cggctccgcg cctgtccttt
     2521 ctccccgcgg gacacacgcg cagactgtcg acggcccccc cgaccgatgt cagcctgggg
     2581 gacgagctcc acttagacgg cgaggacgtg gcgatggcgc atgccgacgc gctagacgat
     2641 ttcgatctgg acatgttggg ggacggggat tccccgggtc cgggatttac cccccacgac
     2701 tccgccccct acggcgctct ggatatggcc gacttcgagt ttgagcagat gtttaccgat
     2761 gcccttggaa ttgacgagta cggtgggcca aagaagaaga ggaaggtgta ggcaacggct
     2821 agcacgacgt gatgcggcag cggccgaatt ccccgggctc gagaagcttg gatccaccgg
     2881 atctagataa ctgatctcga ggcgaatttc cccgatcgtt caaacatttg gcaataaagt
     2941 ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat
     3001 tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt
     3061 atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca
     3121 aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcggga attaattcga
     3181 taagctagag atccgtcaag gtaccagctt ttgttccctt tagtgagggt taatttcgag
     3241 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     3301 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     3361 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     3421 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     3481 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     3541 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     3601 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     3661 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     3721 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     3781 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     3841 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     3901 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     3961 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     4021 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     4081 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     4141 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     4201 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     4261 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     4321 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     4381 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     4441 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     4501 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     4561 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     4621 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     4681 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     4741 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     4801 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     4861 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     4921 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     4981 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     5041 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     5101 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     5161 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     5221 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     5281 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     5341 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     5401 gccacctaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
     5461 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
     5521 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
     5581 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
     5641 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
     5701 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
     5761 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
     5821 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc gccattcagg
     5881 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
     5941 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
     6001 cgttgtaaaa cgacggccag tgaattgtaa tacgactcac tatagggcga attggagctc
     6061 caccgcggag cggagaatta agggagtcag gccttaatta agagctcgca tgcctgcagg
     6121 tccccagatt agccttttca atttcagaaa gaatgctaac ccacagatgg ttagagaggc
     6181 ttacgcagca ggtctcatca agacgatcta cccgagcaat aatctccagg aaatcaaata
     6241 ccttcccaag aaggttaaag atgcagtcaa aagattcagg actaactgca tcaagaacac
     6301 agagaaagat atatttctca agatcagaag tactattcca gtatggacga ttcaaggctt
     6361 gcttcacaaa ccaaggcaag taatagagat tggagtctct aaaaaggtag ttcccactga
     6421 atcaaaggcc atggagtcaa agattcaaat agaggaccta acagaactcg ccgtaaagac
     6481 tggcgaacag ttcatacaga gtctcttacg actcaatgac aagaagaaaa tcttcgtcaa
     6541 catggtggag cacgacacac ttgtctactc caaaaatatc aaagatacag tctcagaaga
     6601 ccaaagggca at
//
