LOCUS       sequence-311058-       11442 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vector for expression of PULSE-controlled EFR-GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11442)
  AUTHORS   Ochoa-Fernandez R, Abel NB, Wieland FG, Schlegel J, Koch LA, Miller 
            JB, Engesser R, Giuriani G, Brandl SM, Timmer J, Weber W, Ott T, 
            Simon R, Zurbriggen MD
  TITLE     Optogenetic control of gene expression in plants in the presence of 
            ambient white light.
  JOURNAL   Nat Methods. 2020 Jul;17(7):717-725. doi: 10.1038/s41592-020-0868-y.
            Epub 2020 Jun 29.
  PUBMED    32601426
REFERENCE   2  (bases 1 to 11442)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-020-0868-y"; journalName: "Nat Methods"; date: 
            "2020-07"; volume: "17"; issue: "7"; pages: "717-725"
COMMENT     Sequence Label: pNBA001
FEATURES             Location/Qualifiers
     source          1..11442
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     538..562
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(3870..3872,3873..4586)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(3912..3933)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4173..4192)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4520..4541
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4877..4901
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6202..6831
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7260..8333
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8399..8593
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     misc_feature    8937..9077
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     8992..9011
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9092..9109)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9263..9851)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9343..9362)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             10274..11065
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga cggattacgg aagacaatac
       61 ggcggcttcg aagtttaaac gattgaatat aaccgacgtg actgttacat ttagggattg
      121 aatataaccg acgtgactgt tacatttagg gattgaatat aaccgacgtg actgttacat
      181 ttagggattg aatataaccg acgtgactgt tacatttagg gataaacgat tgaatataac
      241 cgacgtgact gttacattta gggattgaat ataaccgacg tgactgttac atttagggat
      301 tgaatataac cgacgtgact gttacattta gggattgaat ataaccgacg tgactgttac
      361 atttagggat gctagcctcg agtaggtagc ctttagtcca tgcgttatag gtagccttta
      421 gtccatgcgt tataggtagc ctttagtcca tgcgttatag gtagccttta gtccatgcgt
      481 tataggtagc ctttagtcca tgaagcttag acactagagc tagccctata taagcagagc
      541 tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag
      601 aagacaccgg gaccgatcca gccttctctg gaagcaccat gaagctgtcc ttttcacttg
      661 ttttcaatgc tctcacgttg cttcttcaag tttgcatctt tgctcaagcc aggttttcta
      721 atgagactga tatgcaagct ttgcttgagt tcaagtctca agtttctgaa aacaacaaga
      781 gagaggtctt ggcttcatgg aatcactcct ccccattttg taattggatt ggagtcacat
      841 gtggccgcag gagagaaaga gttataagtt tgaaccttgg aggattcaag ttaaccggtg
      901 tgatctcacc ctccattggt aatctctcct ttcttagatt acttaatctt gcagacaact
      961 cttttggaag taccatccct caaaaggtgg gaaggctatt taggcttcag tacttgaaca
     1021 tgagctataa tcttctcgaa ggaaggattc cgtctagtct ttctaactgc tctagactgt
     1081 cgaccgttga tttatcgtca aaccatcttg gacatggtgt tccttcagaa ctaggttcac
     1141 tttctaagct tgccattctg gatcttagca aaaacaacct tactggaaat tttcctgcat
     1201 ctttaggaaa cttgacgtca cttcagaagc ttgactttgc atataaccag atgagaggtg
     1261 agattccaga cgaagtagct agattgactc aaatggtgtt tttccaaata gcactgaata
     1321 gtttttcagg tggttttcct cctgcattgt acaacatctc ctctcttgag tctctatctc
     1381 tagctgacaa tagcttttcg ggtaatctta gggctgattt tggttatctt ctaccaaatc
     1441 taagaagact tcttttggga acaaatcagt tcactggagc tattcccaaa acacttgcca
     1501 atatctcaag ccttgaaagg tttgatatct catctaatta cctgtctggt agtatccctt
     1561 tgagctttgg aaagttacgt aatctgtggt ggttagggat tcgtaataac tctcttggaa
     1621 ataactcgtc cagtggtctt gaatttattg gggctgtggc gaactgcact caattagagt
     1681 acttagatgt tggttacaat agacttggag gtgagcttcc tgcttctata gccaatctgt
     1741 ccactacatt gactagtctg ttccttggac aaaatcttat ctctggaacc attcctcatg
     1801 acatcgggaa tcttgtaagc ctgcaagaac tcagcttaga aacaaatatg ttgagtggag
     1861 aacttcccgt ctctttcggg aagcttttga acttgcaggt tgtggatctg tattcaaatg
     1921 caatatcggg ggaaatacca tcttattttg gcaacatgac tcggttgcag aagctccatt
     1981 tgaatagcaa tagtttccac ggaagaatcc ctcagagtct tggacgttgt cgatacttgc
     2041 tagacctgtg gatggataca aataggttga atgggactat acctcaggaa atactgcaaa
     2101 ttccatccct cgcttacata gatttgtcaa acaatttctt gacaggccat tttccagaag
     2161 aagttggaaa gttagaactt cttgttggac taggtgcttc gtacaacaaa ttatcaggaa
     2221 agatgccaca agctataggg ggttgtcttt cgatggaatt tctctttatg caaggaaatt
     2281 cgtttgacgg agccattcca gatataagcc ggttggtaag cctaaagaat gttgacttct
     2341 ccaacaacaa tctctctggc cggatacctc gatatctggc cagtcttcct tcgctgcgaa
     2401 atctgaatct ttctatgaac aagtttgagg gaagggtgcc aacaacagga gtgtttcgaa
     2461 atgctacagc agtttctgtt tttggtaaca caaatatttg cggaggcgtc cgagaaatgc
     2521 aactaaagcc atgcattgta caggcatctc caaggaagag aaagcctctg tcagttagaa
     2581 agaaagttgt cagtggtatt tgtataggta tagcttcgct tttgttaatc ataattgtgg
     2641 cttctctgtg ttggttcatg aagaggaaaa agaaaaacaa tgccagtgat ggtaacccat
     2701 ctgattctac tactttgggg atgttccatg agaaggtaag ttatgaagag cttcatagtg
     2761 caacaagtcg cttctcttca accaatttga ttggttcagg caatttcggt aatgtgttta
     2821 aaggattgct tggccctgag aataaactcg tcgcggttaa agttttgaac ctcctaaagc
     2881 atggagcgac gaaaagcttt atggcggaat gtgaaacctt caagggtata cgacatcgta
     2941 accttgtaaa actgataacg gtttgttcaa gccttgattc cgagggaaat gatttcagag
     3001 ctctggtcta tgagttcatg ccaaaaggaa gtctggatat gtggctgcag ctagaagatc
     3061 tggaaagggt aaacgatcac tcgagatctt taacacccgc agagaaactc aacatagcaa
     3121 tagatgtggc ttcagctttg gagtatctgc acgttcattg tcatgaccct gtagctcact
     3181 gtgatattaa gccaagcaac attcttctag acgatgatct gactgctcat gttagtgact
     3241 ttggtttggc tcagctcctc tataaatacg atcgagaatc ctttctaaac cagtttagtt
     3301 ctgctggtgt cagaggcacc attggctatg ccgcgccagg ttaataaaca tctatatgca
     3361 aaaagtattg tttaacaaaa gagttatcca acctcattgt gtgttgtttc tttgaaatat
     3421 gtgcagagta tggaatggga ggccaaccat caatacaagg agatgtgtac agcttcggaa
     3481 ttctactttt ggagatgttt agtggaaaga aaccaacaga tgaatcattt gcaggcgatt
     3541 ataacctcca cagctacaca aagtctatat tatcgggttg cacgagcagt ggaggcagca
     3601 acgccattga tgaggggttg agactggttt tgcaggtggg gataaagtgt tctgaagaat
     3661 atccgaggga taggatgaga acggatgaag cagtacgaga attaatctca ataagatcta
     3721 agttcttcag ttccaagacg actattacag agagtcctcg agatgctccg caaagttctc
     3781 ctcaggaatg gatgttaaat acggacatgc atactatgaa gggaggtgga ggaggttctg
     3841 gaggcggtgg aagtggtggc ggaggtagcg tgagcaaggg cgaggagctg ttcaccgggg
     3901 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     3961 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
     4021 gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt cacctacggc gtgcagtgct
     4081 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     4141 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     4201 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     4261 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
     4321 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
     4381 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     4441 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc
     4501 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     4561 acggcatgga cgagctgtac aagtaaaatc cggccatgct agagtccgca aaaatcacca
     4621 gtctctctct acaaatctat ctctctctat ttttctccag aataatgtgt gagtagttcc
     4681 cagataaggg aattagggtt cttatagggt ttcgctcatg tgttgagcat ataagaaacc
     4741 cttagtatgt atttgtattt gtaaaatact tctatcaata aaatttctaa ttcctaaaac
     4801 caaaatccag tgaccttgag tgacctaagc tagcctagtg tcgggcttgt cttctctggt
     4861 ttaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt
     4921 attagaataa tcggatattt aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat
     4981 gtgcatgcca accacagggt tcccctcggg atcaaagtac tttgatccaa cccctccgct
     5041 gctatagtgc agtcggcttc tgacgttcag tgcagccttc ttctgaaaac gacatgtcgc
     5101 acaagtccta agttacgcga caggctgccg ccctgccctt ttcctggcgt tttcttgtcg
     5161 cgtgttttag tcgcataaag tagaatactt gcgactagaa ccggagacat tacgccatga
     5221 acaagagcgc cgccgctggc ctgctgggct atgcccgcgt cagcaccgac gaccaggact
     5281 tgaccaacca acgggccgaa ctgcacgcgg ccggctgcac caagctgttt tccgagaaga
     5341 tcaccggcac caggcgcgac cgcccggagc tggccaggat gcttgaccac ctacgccctg
     5401 gcgacgttgt gacagtgacc aggctagacc gcctggcccg cagcacccgc gacctactgg
     5461 acattgccga gcgcatccag gaggccggcg cgggcctgcg tagcctggca gagccgtggg
     5521 ccgacaccac cacgccggcc ggccgcatgg tgttgaccgt gttcgccggc attgccgagt
     5581 tcgagcgttc cctaatcatc gaccgcaccc ggagcgggcg cgaggccgcc aaggcccgag
     5641 gcgtgaagtt tggcccccgc cctaccctca ccccggcaca gatcgcgcac gcccgcgagc
     5701 tgatcgacca ggaaggccgc accgtgaaag aggcggctgc actgcttggc gtgcatcgct
     5761 cgaccctgta ccgcgcactt gagcgcagcg aggaagtgac gcccaccgag gccaggcggc
     5821 gcggtgcctt ccgtgaggac gcattgaccg aggccgacgc cctggcggcc gccgagaatg
     5881 aacgccaaga ggaacaagca tgaaaccgca ccaggacggc caggacgaac cgtttttcat
     5941 taccgaagag atcgaggcgg agatgatcgc ggccgggtac gtgttcgagc cgcccgcgca
     6001 catctcaacc gtgcggctgc atgaaatcct ggccggtttg tctgatgcca agctggcggc
     6061 ctggccggcc agcttggccg ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt
     6121 atttgagtaa aacagcttgc gtcatgcggt cgctgcgtat atgatgcgat gagtaaataa
     6181 acaaatacgc aaggggaacg catgaaggtt atcgctgtac ttaaccagaa aggcgggtca
     6241 ggcaagacga ccatcgcaac ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt
     6301 ctgttagtcg attccgatcc ccagggcagt gcccgcgatt gggcggccgt gcgggaagat
     6361 caaccgctaa ccgttgtcgg catcgaccgc ccgacgattg accgcgacgt gaaggccatc
     6421 ggccggcgcg acttcgtagt gatcgacgga gcgccccagg cggcggactt ggctgtgtcc
     6481 gcgatcaagg cagccgactt cgtgctgatt ccggtgcagc caagccctta cgacatatgg
     6541 gccaccgccg acctggtgga gctggttaag cagcgcattg aggtcacgga tggaaggcta
     6601 caagcggcct ttgtcgtgtc gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc
     6661 gaggcgctgg ccgggtacga gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc
     6721 tacccaggca ctgccgccgc cggcacaacc gttcttgaat cagaacccga gggcgacgct
     6781 gcccgcgagg tccaggcgct ggccgctgaa attaaatcaa aactcatttg agttaatgag
     6841 gtaaagagaa aatgagcaaa agcacaaaca cgctaagtgc cggccgtccg agcgcacgca
     6901 gcagcaaggc tgcaacgttg gccagcctgg cagacacgcc agccatgaag cgggtcaact
     6961 ttcagttgcc ggcggaggat cacaccaagc tgaagatgta cgcggtacgc caaggcaaga
     7021 ccattaccga gctgctatct gaatacatcg cgcagctacc agagtaaatg agcaaatgaa
     7081 taaatgagta gatgaatttt agcggctaaa ggaggcggca tggaaaatca agaacaacca
     7141 ggcaccgacg ccgtggaatg ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc
     7201 ggctgggttg cctgccggcc ctgcaatggc actggaaccc ccaagcccga ggaatcggcg
     7261 tgagcggtcg caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg
     7321 tggagaagtt gaaggccgcg caggccgccc agcggcaacg catcgaggca gaagcacgcc
     7381 ccggtgaatc gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg caaccgccgg
     7441 cagccggtgc gccgtcgatt aggaagccgc ccaagggcga cgagcaacca gattttttcg
     7501 ttccgatgct ctatgacgtg ggcacccgcg atagtcgcag catcatggac gtggccgttt
     7561 tccgtctgtc gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag cttccagacg
     7621 ggcacgtaga ggtttccgca gggccggccg gcatggccag tgtgtgggat tacgacctgg
     7681 tactgatggc ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa gggaagggag
     7741 acaagcccgg ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc tgccggcgag
     7801 ccgatggcgg aaagcagaaa gacgacctgg tagaaacctg cattcggtta aacaccacgc
     7861 acgttgccat gcagcgtacg aagaaggcca agaacggccg cctggtgacg gtatccgagg
     7921 gtgaagcctt gattagccgc tacaagatcg taaagagcga aaccgggcgg ccggagtaca
     7981 tcgagatcga gctagctgat tggatgtacc gcgagatcac agaaggcaag aacccggacg
     8041 tgctgacggt tcaccccgat tactttttga tcgatcccgg catcggccgt tttctctacc
     8101 gcctggcacg ccgcgccgca ggcaaggcag aagccagatg gttgttcaag acgatctacg
     8161 aacgcagtgg cagcgccgga gagttcaaga agttctgttt caccgtgcgc aagctgatcg
     8221 ggtcaaatga cctgccggag tacgatttga aggaggaggc ggggcaggct ggcccgatcc
     8281 tagtcatgcg ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg
     8341 agcagatgct agggcaaatt gccctagcag gggaaaaagg tcgaaaagga ctctttcctg
     8401 tggatagcac gtacattggg aacccaaagc cgtacattgg gaaccggaac ccgtacattg
     8461 ggaacccaaa gccgtacatt gggaaccggt cacacatgta agtgactgat ataaaagaga
     8521 aaaaaggcga tttttccgcc taaaactctt taaaacttat taaaactctt aaaacccgcc
     8581 tggcctgtgc ataactgtct ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc
     8641 ttcggtcgct gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc
     8701 gctcaaaaat ggctggccta cggccaggca atctaccagg gcgcggacaa gccgcgccgt
     8761 cgccactcga ccgccggcgc ccacatcaag gcaccctgcc tcgcgcgttt cggtgatgac
     8821 ggtgaaaacc tctgacacat gcagctcccg gagacagtca cagcttgtct gtaagcggat
     8881 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca
     8941 gccatgaccc agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag
     9001 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga
     9061 gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     9121 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     9181 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     9241 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     9301 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     9361 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     9421 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     9481 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     9541 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     9601 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     9661 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     9721 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     9781 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     9841 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcat cggaagaacg
     9901 gcaactaagc tgccgggttt gaaacacgga tgatctcgcg gagggtagca tgttgattgt
     9961 aacgatgaca gagcgttgct gcctgtgatc aattcgggca cgaacccagt ggacataagc
    10021 ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac
    10081 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
    10141 tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg
    10201 tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac
    10261 aaagttaaac atcatggggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt
    10321 agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc
    10381 cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta cggtgaccgt
    10441 aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc
    10501 ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat
    10561 cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga
    10621 cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac
    10681 aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc
    10741 ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc
    10801 gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag
    10861 cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct
    10921 gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga
    10981 agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccactacg tgaaaggcga
    11041 gatcaccaag gtagtcggca aataatgtct agctagaaat tcgttcaagc cgacgccgct
    11101 tcgcggcgcg gcttaactca agcgttagat gcactaagca cataattgct cacagccaaa
    11161 ctatcaggtc aagtctgctt ttattatttt taagcgtgca taataagccc tacacaaatt
    11221 gggagatata tcatgcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
    11281 agaccccgta gaaaagatca aaggatcttc tgccggtaac atgagcaaag tctgccgcct
    11341 tacaacggct ctcccgctga cgccgtcccg gactgatggg ctgcctgtat cgagtggtga
    11401 ttttgtgccg agctgccggt cggggagctg ttggctggct gg
//
