LOCUS       sequence-311059-        8003 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3-color stripe.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 2234 to 2268)
  AUTHORS   Santos-Moreno J, Tasiudi E, Stelling J, Schaerli Y
  TITLE     Multistable and dynamic CRISPRi-based synthetic circuits.
  JOURNAL   Nat Commun. 2020 Jun 2;11(1):2746. doi: 10.1038/s41467-020-16574-1.
  PUBMED    32488086
REFERENCE   2  (bases 1 to 8003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-06-2"; volume: "11(1):2746. doi"; pages: " 
            10.1038/s41467-020-16574-1"
COMMENT     Sequence Label: pJ2042.2
FEATURES             Location/Qualifiers
     source          1..8003
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        48..332
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     255..274
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     protein_bind    393..412
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     protein_bind    515..534
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     protein_bind    595..614
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     protein_bind    721..740
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     protein_bind    807..826
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     RBS             831..853
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             861..1514
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
     promoter        2234..2268
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     protein_bind    2418..2437
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     RBS             2442..2464
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2472..3185
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     promoter        3557..3591
                     /label=BBa_J23105
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.24"
     promoter        3557..3591
                     /label=BBa_J23107
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.36"
     promoter        3557..3591
                     /label=BBa_J23101
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.7"
     protein_bind    3735..3754
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     protein_bind    3861..3880
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     protein_bind    3947..3966
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     RBS             3971..3993
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     terminator      5220..5247
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(5435..6250)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(5435..6250)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     6158..6177
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(6251..6342)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6360..6995)
                     /direction=LEFT
                     /label=ColA ori
                     /note="Plasmids containing the ColA origin of replication 
                     can be propagated in E. coli cells that contain additional 
                     plasmids with compatible origins."
     primer_bind     complement(6505..6524)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7146..8003,1..21))
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 atcattttgc gcttcagcca tacttttcat actcccgcca ttcagagaag aaaccaattg
       61 tccatattgc atcagacatt gccgtcactg cgtcttttac tggctcttct cgctaaccaa
      121 accggtaacc ccgcttatta aaagcattct gtaacaaagc gggaccaaag ccatgacaaa
      181 aacgcgtaac aaaagtgtct ataatcacgg cagaaaagtc cacattgatt atttgcacgg
      241 cgtcacactt tgctatgcca tagcattttt atccataaga ttagcggttc ctacctgacg
      301 ctttttatcg caactctcta ctgtttctcc ataccgaatt cataggatag attctggaaa
      361 ctttaccgtc cgagctccag cctgcggtcc gggttcactg ccgtataggc agaagctaga
      421 ctctagtggt ttcagagcta tgctggaaac agcatagcaa gttgaaataa ggctagtccg
      481 ttatcaactt gaaaaagtgg caccgagtcg gtgcgttcac tgccgtatag gcagtcgctg
      541 ggacgcccgg ggactacact tacgaaacta ttgattgctc agcctgcggt ccgggttcac
      601 tgccgtatag gcagatcagt gtgtactaag tactgtttca gagctatgct ggaaacagca
      661 tagcaagttg aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc
      721 gttcactgcc gtataggcag tcgctgggac gcccgctcga gcaataaaca gttgataggg
      781 cttctccgtt acagcctgcg gtccgggttc actgccgtat aggcagtaat tttgtttaac
      841 tttaagaagg agatatacat atggtttcgg ttatcaaacc agagatgaaa atgcgttact
      901 atatggatgg ttcagtaaat ggtcacgaat ttactattga gggcgagggt acgggacgcc
      961 catacgaggg gcaccaggaa atgactttac gcgtcacaat ggctgaaggc gggcctatgc
     1021 cgtttgcgtt cgatcttgtt agtcatgtct tttgttacgg tcaccgtgta tttactaaat
     1081 accccgagga aattccagac tatttcaaac aagccttccc ggaaggtttg tcttgggagc
     1141 gcagtttaga gtttgaagac ggtggctcgg ccagcgtgtc agctcatatt agtcttcgcg
     1201 gcaatacatt ttatcacaag tcaaagttca ccggcgtgaa cttccccgca gacggcccaa
     1261 tcatgcagaa tcaaagtgtt gattgggaac cgtccacaga gaagattaca gcttccgatg
     1321 gagtcttaaa gggcgatgta accatgtact taaaattaga agggggaggg aaccataaat
     1381 gtcagatgaa gactacctat aaggccgcaa aagagattct tgaaatgccc ggagaccact
     1441 acattgggca tcgtttggtc cgtaagacag aaggaaatat tactgaacag gtcgaagacg
     1501 ctgtggcaca cagcatgtcc cgccgtaata ctgacgccat cacaatccac agcatcctgg
     1561 attggattga agacaacttg gagtcgccgt tgagtttaga aaaagttagt gaacgtagtg
     1621 gttactcaaa gtggcacctt cagcgcatgt ttaagaagga aacgggtcat tcattgggtc
     1681 aatatattcg ttctcgcaag atgactgaaa ttgcccagaa attgaaagag tctaatgaac
     1741 ctattttgta cctggcggag cgttacggct ttgaaagtca gcaaaccctt acacgtacct
     1801 tcaagaatta ctttgacgtt ccaccacaca aatatcgtat gaccaacatg cagggtgagt
     1861 cacgtttttt gcatccgttg aatcattaca attcctaata atcgctggga cgcccgccat
     1921 ggttcagcca aaaaacttaa gaccgccggt cttgtccact accttgcagt aatgcggtgg
     1981 acaggatcgg cggttttctt ttctcttctc aattcttctg acctgtaacg aataatagat
     2041 agtaaagtag tctccgattg agttttctct gccgagtccc acccagttct gtgatttcag
     2101 taagttggta attgatacac tgttgcgaga actgctgcct ggtagtagat aggttgttat
     2161 tgagtaagaa ggtaaagtga acgaaatccc tgaaactgag actgtagaaa ataagcttca
     2221 gcctgcggtc cggttgacgg ctagctcagt cctaggtaca gtgctagctc gctgggacgc
     2281 ccgagatagc cgttacacag gtgacactta tttcagcctg cggtccggcc acactagagt
     2341 ctagcttgag atcgctggga cgcccgggat ccaagagatt tctacacgat tgagcactgt
     2401 ctcagcctgc ggtccgggtt cactgccgta taggcagtaa ttttgtttaa ctttaagaag
     2461 gagatataca tatgcgtaaa ggcgaagaac tgtttaccgg tgtggttccg attctggtgg
     2521 aactggacgg cgatgttaat ggtcataaat tcagtgttcg cggcgaaggt gaaggcgatg
     2581 cgacgaacgg caaactgacc ctgaaattta tctgcaccac gggtaaactg ccggtcccgt
     2641 ggccgacgct ggtgaccacg ctgacctatg gcgttcaatg ttttgcgcgt tacccggatc
     2701 acatgaaaca gcacgacttt ttcaaatcgg ccatgccgga aggctatgtg caggaacgta
     2761 cgattagctt taaagacgat ggtacgtata aaacccgcgc ggaagtgaaa ttcgaaggcg
     2821 ataccctggt taaccgtatc gaactgaaag gtatcgattt caaagaagac ggcaatattc
     2881 tgggtcataa actggaatat aacttcaatt cccacaacgt gtacatcacc gcggataaac
     2941 agaaaaacgg cattaaagcc aatttcaaaa tccgccataa tgtggaagat ggtagcgttc
     3001 agctggccga ccactatcag caaaacacgc cgattggtga tggcccggtc ctgctgccgg
     3061 acaatcacta cctgagtacc cagtccgtgc tgtcaaaaga tccgaacgaa aaacgtgacc
     3121 acatggtcct gctggaattt gtgacggctg cgggtatcac ccacggcatg gacgaactgt
     3181 ataaaatgtc ccgccgtaat actgacgcca tcacaatcca cagcatcctg gattggattg
     3241 aagactaata atcgctggga cgcccgcctg caggctcggt accaaattcc agaaaagagg
     3301 cctcccgaaa ggggggcctt ttttcgtttt ggtcctaata gataaaggat aggtctggta
     3361 gtgttgttcg ttctcgcagg taaatcaata atactcagca gttccgtaga cttttcagtg
     3421 ggacagggta gcgataacag atagattgta ataagacaca gtaggtgctc gtagttgcgt
     3481 gaagagaacc gctcaggaaa tccagtcaga agtattggta atcgttgaaa actcagtcga
     3541 ccagcctgcg gtccggttta cggctagctc agtcctaggt attatgctag ctcgctggga
     3601 cgcccgagtg acgactgcga agtaacctct atttatcagc ctgcggtccg gccaagtact
     3661 tagtacacac tgattcgctg ggacgcccgg tagtgcttat cagacccaat actgttgaac
     3721 agcctgcggt ccgggttcac tgccgtatag gcagtctcaa gctagactct agtggtttca
     3781 gagctatgct ggaaacagca tagcaagttg aaataaggct agtccgttat caacttgaaa
     3841 aagtggcacc gagtcggtgc gttcactgcc gtataggcag tcgctgggac gcccggacgt
     3901 cctattacac tcgtcgttgg aaactgaaga tcagcctgcg gtccgggttc actgccgtat
     3961 aggcagtaat tttgtttaac tttaagaagg agatatacat atgaatcagt cattcatctc
     4021 ggacatctta tatgccgaca tcgaatcgaa ggctaaggaa cttacagtca attccaacaa
     4081 tactgtccag ccggtcgcgc ttatgcgctt aggagttttc gttcccaaac cttccaagag
     4141 caaaggagaa agtaaggaaa ttgacgccac caaagccttc tctcaactgg agattgctaa
     4201 agcagagggc atggttagta aaggagaaga aaataacatg gcactgatta aggagaacat
     4261 gcacatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga
     4321 gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggccg tcgagggcgg
     4381 ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca gcaaaacctt
     4441 catcaaccac acccagggca tccccgactt ctttaagcag tccttccctg agggcttcac
     4501 atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag
     4561 cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact tcccatccaa
     4621 cggccctgtg atgcagaaga aaacactcgg ctgggaggcc tccaccgaga ccctgtaccc
     4681 cgctgacggc ggcctggaag gcagagccga catggccctg aagctcgtgg gcgggggcca
     4741 cctgatctgc aacttgaaga ccacatacag atccaagaaa cccgctaaga acctcaagat
     4801 gcccggcgtc tactatgtgg acagaagact ggaaagaatc aaggaggccg acaaagagac
     4861 ctacgtcgag cagcacgagg tggctgtggc cagatactgc gacctcccta gcaaactggg
     4921 gcacagagtc taataatcgc tgggacgccc ggcggccgcg gaaacacaga aaaaagcccg
     4981 cacctgacag tgcgggcttt ttttttcgac caaaggtagc gaacgacgag tcactgttga
     5041 ggataaatac tttctctact aggcgcctgt tacacaggtc ctcagcggcg cgcctttgtc
     5101 ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg gatttgaacg ttgtgaagca
     5161 acggcccgga gggtggcggg caggacgccc gccataaact gccaggcatc aaactaagca
     5221 gaaggccatc ctgacggatg gcctttttgc gtttcagatc taccggtaaa ccagcaatag
     5281 acataagcgg ctatttaacg accctgccct gaaccgacga caagctgacg accgggtctc
     5341 cgcaagtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     5401 acattcaaat atgtatccgc tcatgaatta attcttagaa aaactcatcg agcatcaaat
     5461 gaaactgcaa tttattcata tcaggattat caataccata tttttgaaaa agccgtttct
     5521 gtaatgaagg agaaaactca ccgaggcagt tccataggat ggcaagatcc tggtatcggt
     5581 ctgcgattcc gactcgtcca acatcaatac aacctattaa tttcccctcg tcaaaaataa
     5641 ggttatcaag tgagaaatca ccatgagtga cgactgaatc cggtgagaat ggcaaaagtt
     5701 tatgcatttc tttccagact tgttcaacag gccagccatt acgctcgtca tcaaaatcac
     5761 tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg agcgagacga aatacgcggt
     5821 cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca
     5881 gcgcatcaac aatattttca cctgaatcag gatattcttc taatacctgg aatgctgttt
     5941 tcccggggat cgcagtggtg agtaaccatg catcatcagg agtacggata aaatgcttga
     6001 tggtcggaag aggcataaat tccgtcagcc agtttagtct gaccatctca tctgtaacat
     6061 cattggcaac gctacctttg ccatgtttca gaaacaactc tggcgcatcg ggcttcccat
     6121 acaatcgata gattgtcgca cctgattgcc cgacattatc gcgagcccat ttatacccat
     6181 ataaatcagc atccatgttg gaatttaatc gcggcctaga gcaagacgtt tcccgttgaa
     6241 tatggctcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     6301 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggcatg ctagcgcaga
     6361 aacgtcctag aagatgccag gaggatactt agcagagaga caataaggcc ggagcgaagc
     6421 cgtttttcca taggctccgc ccccctgacg aacatcacga aatctgacgc tcaaatcagt
     6481 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgat ggctccctct
     6541 tgcgctctcc tgttcccgtc ctgcggcgtc cgtgttgtgg tggaggcttt acccaaatca
     6601 ccacgtcccg ttccgtgtag acagttcgct ccaagctggg ctgtgtgcaa gaaccccccg
     6661 ttcagcccga ctgctgcgcc ttatccggta actatcatct tgagtccaac ccggaaagac
     6721 acgacaaaac gccactggca gcagccattg gtaactgaga attagtggat ttagatatcg
     6781 agagtcttga agtggtggcc taacagaggc tacactgaaa ggacagtatt tggtatctgc
     6841 gctccactaa agccagttac caggttaagc agttccccaa ctgacttaac cttcgatcaa
     6901 accgcctccc caggcggttt tttcgtttac agagcaggag attacgacga tcgtaaaagg
     6961 atctcaagaa gatcctttac ggattcccga caccatcact ctagatttca gtgcaattta
     7021 tctcttcaaa tgtagcacct gaagtcagcc ccatacgata taagttgtaa ttctcatgtt
     7081 agtcatgccc cgcgcccacc ggaaggagct gactgggttg ctcctagggt ctgattcgtt
     7141 accaattatg acaacttgac ggctacatca ttcacttttt cttcacaacc ggcacggaac
     7201 tcgctcgggc tggccccggt gcatttttta aatacccgcg agaaatagag ttgatcgtca
     7261 aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa aagcagcttc
     7321 gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa ctgctggcgg
     7381 aaaagatgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct ggcgatatca
     7441 aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac ccgattatcc
     7501 atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa ttgctcaagc
     7561 agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt aatgatttgc
     7621 ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa ccccgtattg
     7681 gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg aaagtaaacc
     7741 cactggtgat accattcgcg agcctccgga tgacgaccgt agtgatgaat ctctcctggc
     7801 gggaacagca aaatatcacc cggtcggcaa acaaattctc gtccctgatt tttcaccacc
     7861 ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg tcggtcgata
     7921 aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag atgggcatta
     7981 aacgagtatc ccggcagcag ggg
//
