LOCUS       sequence-311064-       12160 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Independent expression of QuasAr and human Kvbeta 1 in pFCK vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12160)
  AUTHORS   Cho IH, Panzera LC, Chin M, Alpizar SA, Olveda GE, Hill RA, Hoppa MB
  TITLE     The potassium channel subunit K(v)beta1 serves as a major control 
            point for synaptic facilitation.
  JOURNAL   Proc Natl Acad Sci U S A. 2020 Nov 24;117(47):29937-29947. doi: 
            10.1073/pnas.2000790117. Epub 2020 Nov 9.
  PUBMED    33168717
REFERENCE   2  (bases 1 to 12160)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.2000790117"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2020-11-24- 24"; volume: "117"; issue: "47"; pages: 
            "29937-29947"
COMMENT     Sequence Label: QuasAr-P2A-hKvb1
FEATURES             Location/Qualifiers
     source          1..12160
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1443..1676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1861..1905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2203..2320
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2365..3653
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     CDS             3666..4424
                     /codon_start=1
                     /product="achaerhodopsin-3 (Arch) derivative with improved 
                     voltage sensitivity (Hochbaum et al., 2014)"
                     /label=QuasAr2
                     /translation="MVSIALQAGYDLLGDGRPETLWLGIGTLLMLIGTFYFLVRGWGVT
                     DKDAREYYAVTILVSGIASAAYLSMFFGIGLTEVSVGGEMLDIYYARYAQWLFTTPLLL
                     LHLALLAKVDRVTIGTLVGVDALMIVTGLIGALSHTAIARYSWWLFSTICMIVVLYVLA
                     TSLRSAAKERGPEVASTFNTLTALVLVLWTAYPILWIIGTEGAGVVGLGIETLLFMVLD
                     VTAKVGFGFILLRSRAILGDTEAPEPSAGAD"
     CDS             4431..5138
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEYNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     CDS             5235..5291
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6513..6542
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             6561..6584
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    6615..7203
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6668..6688)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7086..7097)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7206..7222)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7207..7223)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7728..7908
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7934..7951)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7940..8164
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8210..8638
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8297..8316)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8507..8528
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8647..8667)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8652..8981
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8832..8967
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8894..8913
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9029..9076
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9095..9469
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9599..9720
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9636..9655)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9690..9709
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9769..9785)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9769..9785)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9782..9804)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9793..9809
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9817..9823,9824..9841,9842..9847))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9862..9883
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10000..10017)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10171..10759)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10251..10270)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10930..11721,11722..11790))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11553..11572
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11791..11895)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11970..11989)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2341 cagagatcca gtttggttaa ttaacattat ggccttaggt cacttcatct ccatggggtt
     2401 cttcttctga ttttctagaa aatgagatgg gggtgcagag agcttcctca gtgacctgcc
     2461 cagggtcaca tcagaaatgt cagagctaga acttgaactc agattactaa tcttaaattc
     2521 catgccttgg gggcatgcaa gtacgatata cagaaggagt gaactcatta gggcagatga
     2581 ccaatgagtt taggaaagaa gagtccaggg cagggtacat ctacaccacc cgcccagccc
     2641 tgggtgagtc cagccacgtt cacctcatta tagttgcctc tctccagtcc taccttgacg
     2701 ggaagcacaa gcagaaactg ggacaggagc cccaggagac caaatcttca tggtccctct
     2761 gggaggatgg gtggggagag ctgtggcaga ggcctcagga ggggccctgc tgctcagtgg
     2821 tgacagatag gggtgagaaa gcagacagag tcattccgtc agcattctgg gtctgtttgg
     2881 tacttcttct cacgctaagg tggcggtgtg atatgcacaa tggctaaaaa gcagggagag
     2941 ctggaaagaa acaaggacag agacagaggc caagtcaacc agaccaattc ccagaggaag
     3001 caaagaaacc attacagaga ctacaagggg gaagggaagg agagatgaat tagcttcccc
     3061 tgtaaacctt agaacccagc tgttgccagg gcaacggggc aatacctgtc tcttcagagg
     3121 agatgaagtt gccagggtaa ctacatcctg tctttctcaa ggaccatccc agaatgtggc
     3181 acccactagc cgttaccata gcaactgcct ctttgcccca cttaatccca tcccgtctgt
     3241 taaaagggcc ctatagttgg aggtggggga ggtaggaaga gcgatgatca cttgtggact
     3301 aagtttgttc gcatcccctt ctccaacccc ctcagtacat caccctgggg gaacagggtc
     3361 cacttgctcc tgggcccaca cagtcctgca gtattgtgta tataaggcca gggcaaagag
     3421 gagcaggttt taaagtgaaa ggcaggcagg tgttggggag gcagttaccg gggcaacggg
     3481 aacagggcgt ttcggaggtg gttgccatgg ggacctggat gctgacgaag gctcgcgagg
     3541 ctgtgagcag ccacagtgcc ctgctcagaa gccccaagct cgtcagtcaa gccggttctc
     3601 cgtttgcact caggagcacg ggcaggcgag tggcccctag ttctgggggc agcgggggat
     3661 ccaccatggt aagtatcgct ctgcaggctg gttacgacct actgggtgac ggcagacctg
     3721 aaaccctgtg gctgggcatc ggcactctgc tgatgctgat tggaaccttc tactttctgg
     3781 tccgcggatg gggagtcacc gataaggatg cccgggaata ttacgctgtg actatcctgg
     3841 tgtccggaat cgcatccgcc gcatatctgt ctatgttctt tggtatcggg cttactgagg
     3901 tgtccgtcgg gggcgaaatg ttggatatct attatgccag gtacgcccag tggctgttta
     3961 ccaccccact tctgctgctg cacctggccc ttctcgctaa ggtggatcgg gtgaccatcg
     4021 gcaccctggt gggtgtggac gccctgatga tcgtcactgg cctcatcgga gccttgagcc
     4081 acacggccat agccagatac agttggtggt tgttctctac aatttgcatg atagtggtgc
     4141 tctatgttct ggctacatcc ctgcgatctg ctgcaaagga gcggggcccc gaggtggcat
     4201 ctacctttaa caccctgaca gctctggtct tggtgctgtg gaccgcttac cctatcctgt
     4261 ggatcatagg cactgagggc gctggcgtgg tgggcctggg catcgaaact ctgctgttta
     4321 tggtgttgga cgtgactgcc aaggtcggct ttggctttat cctgttgaga tcccgggcta
     4381 ttctgggcga caccgaggca ccagaaccca gtgccggtgc cgatacgttg atggtgagca
     4441 agggcgagga gtataacatg gccatcatca aggagttcat gcgcttcaag gtgcgcatgg
     4501 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
     4561 agggctttca gaccgctaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg
     4621 acatcctgtc ccctcatttc acctacggct ccaaggccta cgtgaagcac cccgccgaca
     4681 tccccgacta cttcaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
     4741 acgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca
     4801 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgtg atgcagaaga
     4861 agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggt gccctgaagg
     4921 gcaagatcaa gatgaggctg aagctgaagg acggcggcca ctacacctcc gaggtcaaga
     4981 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacatcgtc gacatcaagt
     5041 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg
     5101 gccgccactc caccggcggc atggacgagc tgtacaagag tagaatcaca agcgaaggcg
     5161 agtacatccc cctggatcaa atagacataa atgtaggtgg attttgttat gagaatgaag
     5221 aattcggaag cggagctact aacttcagcc tgctgaagca ggctggagac gtggaggaga
     5281 accctggacc tatgcaagtc tccatagcct gcacagagca caatttgaag agtcggaatg
     5341 gtgaggaccg acttctgagc aagcagagct ccaccgcccc caatgtggtg aacgcagccc
     5401 gggccaaatt ccgcacggtc gctatcatcg cgcgcagcct ggggacgttc acgcctcagc
     5461 atcacatttc tctcaaagag tccaccgcaa agcagactgg catgaaatat aggaatcttg
     5521 gaaaatcagg actcagagtt tcttgcttgg gtcttggaac atgggtgaca tttggaggtc
     5581 aaatttcaga tgaggttgct gaacggctga tgaccatcgc ctatgaaagt ggtgttaacc
     5641 tctttgatac tgccgaagtc tatgctgctg gaaaggctga agtgattctg gggagcatca
     5701 tcaagaagaa aggctggagg aggtccagtc tggtcataac aaccaaactc tactggggtg
     5761 gaaaagctga aacagaaaga gggctgtcaa gaaagcatat tattgaagga ttgaagggct
     5821 ccctccagag gctgcagctc gagtatgtgg atgtggtctt tgcaaatcga ccggacagta
     5881 acactcccat ggaagaaatt gtccgagcca tgacacatgt gataaaccaa ggcatggcga
     5941 tgtactgggg cacctcgaga tggagtgcta tggagatcat ggaagcctat tctgtagcaa
     6001 gacagttcaa tatgatccca ccggtctgtg aacaagctga gtaccatctt ttccagagag
     6061 agaaagtgga ggtccagctg ccagagctct accacaaaat aggtgttggc gcaatgacat
     6121 ggtctccact tgcctgtgga atcatctcag gaaaatacgg aaacggggtg cctgaaagtt
     6181 ccagggcttc actgaagtgc taccagtggt tgaaagaaag aattgtaagt gaagaaggga
     6241 gaaaacagca aaacaagcta aaagaccttt ccccaattgc ggagcgtctg ggatgcacac
     6301 tacctcagct agctgttgcg tggtgcctga gaaatgaagg tgtgagttct gtgctcctgg
     6361 gatcatccac tcctgaacaa ctcattgaaa accttggtgc cattcaggtt ctcccaaaga
     6421 tgacatcaca tgtggtaaat gagattgata acatactgcg caacaagccc tacagcaaga
     6481 aggactatag atcaacgcgt acgcggccgc tcgagcagaa actcatctca gaagaggatc
     6541 tggcagcaaa tgatatcctg gattacaagg atgacgacga taaggtttaa gaattcgata
     6601 tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     6661 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     6721 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     6781 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     6841 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     6901 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     6961 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     7021 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     7081 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     7141 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     7201 cgcatcgata ccgtcgacct cgagacctag aaaaacatgg agcaatcaca agtagcaata
     7261 cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt
     7321 ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct gtagatctta
     7381 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     7441 atatccttga tctgtggatc taccacacac aaggctactt ccctgattgg cagaactaca
     7501 caccagggcc agggatcaga tatccactga cctttggatg gtgctacaag ctagtaccag
     7561 ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa cacccgcttg ttacaccctg
     7621 tgagcctgca tgggatggat gacccggaga gagaagtatt agagtggagg tttgacagcc
     7681 gcctagcatt tcatcacatg gcccgagagc tgcatccgga ctgtactggg tctctctggt
     7741 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     7801 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     7861 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagg gcccgtttaa
     7921 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     7981 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     8041 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     8101 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     8161 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt
     8221 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     8281 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     8341 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     8401 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     8461 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     8521 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     8581 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa
     8641 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     8701 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     8761 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     8821 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     8881 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
     8941 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta
     9001 tatccatttt cggatctgat cagcacgtgt tgacaattaa tcatcggcat agtatatcgg
     9061 catagtataa tacgacaagg tgaggaacta aaccatggcc aagttgacca gtgccgttcc
     9121 ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt
     9181 ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt
     9241 catcagcgcg gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg
     9301 cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc
     9361 ctccgggccg gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg
     9421 cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag caggactgac acgtgctacg
     9481 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     9541 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
     9601 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     9661 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     9721 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
     9781 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     9841 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     9901 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     9961 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
    10021 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
    10081 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
    10141 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
    10201 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
    10261 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
    10321 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
    10381 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
    10441 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
    10501 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
    10561 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
    10621 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
    10681 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
    10741 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
    10801 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
    10861 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
    10921 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
    10981 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
    11041 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
    11101 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
    11161 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
    11221 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
    11281 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
    11341 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
    11401 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
    11461 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
    11521 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
    11581 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    11641 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
    11701 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    11761 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    11821 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    11881 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga
    11941 tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
    12001 cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa
    12061 gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt
    12121 tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt
//
